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Tissue engineering is an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function. The aims of this work were to compare chemically and physically processed human Amniotic Membranes (hAM) and analyze the cytocompatibility and proliferation rate (PR) of two primary human mesenchymal stromal cell lines, from different sources and donor conditions seeded over these scaffolds. The evaluated hAM processes were: cold shock to obtain a frozen amniotic membrane (FEAM) with remaining dead epithelial cells, denudation of hAM with trypsin for 20/10 min (DEAM20/10) or treatment with sodium dodecyl sulfate to decellularized hAM (DAM). All samples were sterilized with gamma radiation. The selection of the treated hAM to then generate composites was performed by scanning and transmission electron microscopy and characterization by X-ray diffraction, selecting DEAM10 and FEAM as scaffolds for cell seeding. Two sources of primary human stromal cells were used, both developed by our researchers, human Dental Pulp Stem Cells (hDPSC) from living donors and human Mesenchymal Stromal Cells (hMSC) from bone marrow isolated from brain dead donors. This last line of cells conveys a novel source of human cells that, to our knowledge, have not been tested as part of this type of construct. We developed four in vitro constructs without cytotoxicity signs and with different PR depending on the scaffolds and cells. hDPSC and hMSC grew over both FEAM and DEAM10, but DEAM10 allowed higher PR.
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Tissue engineering (TE) and regenerative medicine offer strategies to improve damaged tissues by using scaffolds and cells. The use of collagen-based biomaterials in the field of TE has been intensively growing over the past decades. Mesenchymal stromal cells (MSCs) and dental pulp stem cells (DPSCs) are promising cell candidates for development of clinical composites. In this study, we proposed the development of a bovine collagen type I: chondroitin-6-sulphate (CG) scaffold, obtained from Uruguayan raw material (certified as free bovine spongiform encephalopathy), with CG crosslinking enhancement using different gamma radiation doses. Structural, biomechanical and chemical characteristics of the scaffolds were assessed by Scanning Electron Microscopy, axial tensile tests, FT-IR and Raman Spectroscopy, respectively. Once we selected the most appropriate scaffold for future use as a TE product, we studied the behavior of MSCs and DPSCs cultured on the scaffold by cytotoxicity, proliferation and differentiation assays. Among the diverse porous scaffolds obtained, the one with the most adequate properties was the one exposed to 15 kGy of gamma radiation. This radiation dose contributed to the crosslinking of molecules, to the formation of new bonds and/or to the reorganization of the collagen fibers. The selected scaffold was non-cytotoxic for the tested cells and a suitable substrate for cell proliferation. Furthermore, the scaffold allowed MSCs differentiation to osteogenic, chondrogenic, and adipogenic lineages. Thus, this work shows a promising approach to the synthesis of a collagen-scaffold suitable for TE.
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We report on unusual magnetic properties observed for nanofluid room temperature ferromagnetic graphite (with an average particle size of [Formula: see text] nm). More precisely, the measured magnetization exhibits a low temperature anomaly (attributed to the manifestation of finite size effects below the quantum temperature [Formula: see text]) as well as pronounced temperature oscillations above T = 50 K (attributed to manifestation of the hard-sphere type of pair correlations between ferromagnetic particles in the nanofluid).
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Antecedentes: A pesar del gran número de autores que se refieren a esta complicación, no hay aún hoy acuerdo sobre causas. Objetivo: Reconocer los factores que favorecen su desarrollo. Lugar de aplicación: Hospital público de atención terciaria de pacientes oncológicos. Diseño: Prospectivo no randomizado. Población: 100 enfermos, 93 por ciento masculinos, edad media 59,13 años, 98 por ciento portadores de carcinoma epidermoide sometidos a laringectomía total. Método: Análisis uni y multivariado de 21 variables potencialmente favorecedoras de fístulas postoperatorias. Resultados: Ninguna de las vriables resultó estadísticamente significativa. La radioterapia previa se comportó como el factor de mayor probabilidad del evento. La incidencia de fístulas alcanzó el 55 por ciento de los operados. Su momento de aparición tuvo una media de 9 días. El 76,3 por ciento cerró espontáneamente. Las peores consecuencias del evento resultaron la prolongación en el uso 3,6 veces mayor a la media de la sonda de alimentación, la necesidad de nuevos tiempos quirúrgicos en 13 pacientes y la rehabilitación 2 meses después que el resto. Conclusiones: La dimensión de la muestra resultó insuficiente para desarrollar el modelo predictivo del fenómeno
Assuntos
Adulto , Masculino , Humanos , Feminino , Pessoa de Meia-Idade , Fístula , Laringectomia , Complicações Pós-Operatórias , Análise Multivariada , Causalidade , Neoplasias Laríngeas , Faringe , Estudos Retrospectivos , Fatores de RiscoRESUMO
Antecedentes: A pesar del gran número de autores que se refieren a esta complicación, no hay aún hoy acuerdo sobre causas. Objetivo: Reconocer los factores que favorecen su desarrollo. Lugar de aplicación: Hospital público de atención terciaria de pacientes oncológicos. Diseño: Prospectivo no randomizado. Población: 100 enfermos, 93 por ciento masculinos, edad media 59,13 años, 98 por ciento portadores de carcinoma epidermoide sometidos a laringectomía total. Método: Análisis uni y multivariado de 21 variables potencialmente favorecedoras de fístulas postoperatorias. Resultados: Ninguna de las vriables resultó estadísticamente significativa. La radioterapia previa se comportó como el factor de mayor probabilidad del evento. La incidencia de fístulas alcanzó el 55 por ciento de los operados. Su momento de aparición tuvo una media de 9 días. El 76,3 por ciento cerró espontáneamente. Las peores consecuencias del evento resultaron la prolongación en el uso 3,6 veces mayor a la media de la sonda de alimentación, la necesidad de nuevos tiempos quirúrgicos en 13 pacientes y la rehabilitación 2 meses después que el resto. Conclusiones: La dimensión de la muestra resultó insuficiente para desarrollar el modelo predictivo del fenómeno (AU)
Assuntos
Adulto , Masculino , Humanos , Feminino , Pessoa de Meia-Idade , Idoso , Laringectomia/efeitos adversos , Fístula/etiologia , Complicações Pós-Operatórias , Estudos Retrospectivos , Causalidade , Neoplasias Laríngeas/cirurgia , Análise Multivariada , Fatores de Risco , FaringeRESUMO
The serious neuropsychological repercussions of hepatic encephalopathy have led to the creation of several experimental models in order to better understand the pathogenesis of the disease. In the present investigation, two possible causes of hepatic encephalopathy, cholestasis and portal hypertension, were chosen to study the behavioral impairments caused by the disease using an object recognition task. This working memory test is based on a paradigm of spontaneous delayed non-matching to sample and was performed 60 days after surgery. Male Wistar rats (225-250 g) were divided into three groups: two experimental groups, microsurgical cholestasis (N = 20) and extrahepatic portal hypertension (N = 20), and a control group (N = 20). A mild alteration of the recognition memory occurred in rats with cholestasis compared to control rats and portal hypertensive rats. The latter group showed the poorest performance on the basis of the behavioral indexes tested. In particular, only the control group spent significantly more time exploring novel objects compared to familiar ones (P < 0.001). In addition, the portal hypertension group spent the shortest time exploring both the novel and familiar objects (P < 0.001). These results suggest that the existence of portosystemic collateral circulation per se may be responsible for subclinical encephalopathy.
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Colestase/complicações , Modelos Animais de Doenças , Encefalopatia Hepática/etiologia , Hipertensão Portal/complicações , Memória/fisiologia , Reconhecimento Visual de Modelos/fisiologia , Reconhecimento Psicológico/fisiologia , Animais , Comportamento Exploratório/fisiologia , Encefalopatia Hepática/fisiopatologia , Encefalopatia Hepática/psicologia , Masculino , Ratos , Ratos Wistar , Análise e Desempenho de Tarefas , Fatores de TempoRESUMO
The serious neuropsychological repercussions of hepatic encephalopathy have led to the creation of several experimental models in order to better understand the pathogenesis of the disease. In the present investigation, two possible causes of hepatic encephalopathy, cholestasis and portal hypertension, were chosen to study the behavioral impairments caused by the disease using an object recognition task. This working memory test is based on a paradigm of spontaneous delayed non-matching to sample and was performed 60 days after surgery. Male Wistar rats (225-250 g) were divided into three groups: two experimental groups, microsurgical cholestasis (N = 20) and extrahepatic portal hypertension (N = 20), and a control group (N = 20). A mild alteration of the recognition memory occurred in rats with cholestasis compared to control rats and portal hypertensive rats. The latter group showed the poorest performance on the basis of the behavioral indexes tested. In particular, only the control group spent significantly more time exploring novel objects compared to familiar ones (P < 0.001). In addition, the portal hypertension group spent the shortest time exploring both the novel and familiar objects (P < 0.001). These results suggest that the existence of portosystemic collateral circulation per se may be responsible for subclinical encephalopathy.
Assuntos
Animais , Masculino , Ratos , Colestase/complicações , Modelos Animais de Doenças , Encefalopatia Hepática/etiologia , Hipertensão Portal/complicações , Memória/fisiologia , Reconhecimento Visual de Modelos/fisiologia , Reconhecimento Psicológico/fisiologia , Comportamento Exploratório/fisiologia , Encefalopatia Hepática/fisiopatologia , Encefalopatia Hepática/psicologia , Ratos Wistar , Análise e Desempenho de Tarefas , Fatores de TempoRESUMO
Postoperative infection has influence on costs, quality of life, and outcome of the disease. It is suspected that post-total laryngectomy infections have increased in frequency and seriousness, because of the failure of the preservation protocol or the previous radiotherapy, making rescue surgery necessary. The objective of this study was to develop a predictive model of infection based on the pre- and intrasurgical variables considered risky. One hundred fifty five patients with E III-IV laryngeal cancer, with 24.8:1 male to female ratio (mean age, 58 years) who underwent total laryngectomy were evaluated for uni- and multivariate analysis of age, sex, histological grade, primary or recurrent disease, tobacco, alcohol, diabetes, tuberculosis/chronic emphysema, red and white cell counts, erythrosedimentation rate (ESR), albumin, chemotherapy, neck radiotherapy and/or previous surgery, confinement days, type and time of surgery, which were factors in the infection event. A predictive model of infection was developed and included albuminemia (<3.5 g%), >1 liter of alcohol daily, and exclusive surgery of the primary. The sensitivity was 90.5% and the specificity 68%. The variance reached 29.6%. The causes of infection were multiple, having analyzed only 30% of them. However, the resulting model was classified correctly in 83.2% of cases. A careful preoperative assessment, an adjusted planning of the surgery, an appropriate use of antibiotics, and a meticulous operative technique are needed to prevent infection.
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Neoplasias Laríngeas/cirurgia , Laringectomia , Infecção da Ferida Cirúrgica/etiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Distribuição de Qui-Quadrado , Feminino , Humanos , Período Intraoperatório , Tempo de Internação/estatística & dados numéricos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Curva ROC , Fatores de Risco , Sensibilidade e Especificidade , Infecção da Ferida Cirúrgica/mortalidade , Infecção da Ferida Cirúrgica/prevenção & controleRESUMO
A new solitary extramedullary plasmacytoma of nasal localization meeting the conditions for definition is reported. The patient was treated with surgery and irradiation, which controlled the disease and produced excellent cosmetic results. The bibliography is reviewed and the pathologic, clinical, and evolutive characteristics are discussed.
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Neoplasias Nasais/diagnóstico , Plasmocitoma/diagnóstico , Anticorpos Monoclonais/imunologia , Terapia Combinada , Humanos , Masculino , Pessoa de Meia-Idade , Nariz/diagnóstico por imagem , Nariz/patologia , Nariz/cirurgia , Neoplasias Nasais/imunologia , Neoplasias Nasais/terapia , Plasmocitoma/imunologia , Plasmocitoma/terapia , Tomografia Computadorizada por Raios XRESUMO
Trichlorooxo[1,3-propanediylbis(diphenylphosphine)-P,P ']rhenium(V), [ReCl(3)O(C(27)H(26)P(2))], crystallizes with four formula units per unit cell. The crystal structure consists of neutral complexes of [ReOCl(3)(dppp)] [dppp is 1,3-bis(diphenylphosphino)propane] packed by H.pi-ring interactions. The Re atom is octahedrally coordinated to the oxo anion, three Cl atoms and two P atoms from the dppp ligand. The six-membered ring formed by the bidentate dppp ligand and the rhenium metal centre is in a chair conformation. The title compound is an intermediate in the synthesis of bis(dppp) complexes of rhenium.
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Compostos Organometálicos/química , Cristalografia por Raios X , Modelos Moleculares , Conformação MolecularRESUMO
The structures of two absorption furosemide prodrugs, hexanoyloxymethyl 4-chloro-N-furfuryl-5-sulfamoyl-anthranilate (C19H23CIN2O7S), (I), and benzoyloxymethyl 4-chloro-N-furfuryl-5-sulfamoylanthranilate (C20H17CIN2O7S), (II), are described in this paper and compared with furosemide and four other prodrugs. The molecular conformations of both compounds are similar to those of the other prodrugs; the packing and the crystal system are the primary differences. Compound (I) crystallizes in the trigonal space group R3 and compound (II) in the monoclinic space group P2(1)/n. The packing of both structures is stabilized by a three-dimensional hydrogen-bond network.
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Cristalografia por Raios X , Furosemida/farmacocinética , Absorção Intestinal , Pró-Fármacos/farmacocinética , Ligação de Hidrogênio , Modelos MolecularesRESUMO
Surgery in 227 cases of urinary stress incontinence (USI) associated to other abdominal and/or vaginal pathologies, by means Burch colposuspension, has given a success rate of 90.3% recovery of USI 6.6% improved urinary incontinence grade, an 98.3% recovery rate of the concomitant genital prolapse, in a follow-up period mean of 42 moths. Postoperative complication of the surgical procedure are analized.