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1.
Braz. j. infect. dis ; Braz. j. infect. dis;20(6): 556-563, Nov.-Dec. 2016. tab
Artigo em Inglês | LILACS | ID: biblio-828166

RESUMO

ABSTRACT Background: Carbapenem-resistant Acinetobacter baumannii (CRAb) is an important cause of nosocomial infections especially in intensive care units. This study aimed to assess clinical aspects and the genetic background of CRAb among ICU patients at a Brazilian teaching hospital. Methods: 56 critically ill patients colonized or infected by CRAb, during ICU stay, were prospectively assessed. Based on imipenem MIC ≥ 4 µg/mL, 28 CRAB strains were screened for the presence of genes encoding metallo-β-lactamases and OXA-type β-lactamases. The blaOXA-type genes were characterized by PCR using primers targeting ISAba-1 or -3. Genetic diversity of blaOXA-positive strains was determined by ERIC-PCR analysis. Results: Patient's mean age (±SD) was 61 (±15.1), and 58.9% were male. Eighty-percent of the patients presented risk factors for CRAb colonization, mainly invasive devices (87.5%) and previous antibiotic therapy (77.6%). Thirty-three patients died during hospital stay (59.0%). Resistance to carbapenems was associated with a high prevalence of blaOXA-23 (51.2%) and/or blaOXA-143 (18.6%) genes. ERIC-PCR genotyping identified 10 clusters among OXA-producing CRAb. Three CRAb strains exhibited additional resistance to polymyxin B (MIC ≥ 4 µg/mL), whereas 10 CRAb strains showed tigecycline MICs > 2 µg/mL. Conclusions: In this study, clonally unrelated OXA-123- and OXA-143-producing A. baumannii strains in ICU patients were strongly correlated to colonization with infected patients being associated with a poor outcome.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , beta-Lactamases/biossíntese , Infecções por Acinetobacter/microbiologia , Infecção Hospitalar/microbiologia , Acinetobacter baumannii/enzimologia , Antibacterianos/farmacologia , beta-Lactamases/genética , Brasil , Testes de Sensibilidade Microbiana , Estudos Prospectivos , Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/genética , Reação em Cadeia da Polimerase Multiplex , Genótipo , Hospitais de Ensino , Unidades de Terapia Intensiva
2.
Braz J Infect Dis ; 20(6): 556-563, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27620658

RESUMO

BACKGROUND: Carbapenem-resistant Acinetobacter baumannii (CRAb) is an important cause of nosocomial infections especially in intensive care units. This study aimed to assess clinical aspects and the genetic background of CRAb among ICU patients at a Brazilian teaching hospital. METHODS: 56 critically ill patients colonized or infected by CRAb, during ICU stay, were prospectively assessed. Based on imipenem MIC≥4µg/mL, 28 CRAB strains were screened for the presence of genes encoding metallo-ß-lactamases and OXA-type ß-lactamases. The blaOXA-type genes were characterized by PCR using primers targeting ISAba-1 or -3. Genetic diversity of blaOXA-positive strains was determined by ERIC-PCR analysis. RESULTS: Patient's mean age (±SD) was 61 (±15.1), and 58.9% were male. Eighty-percent of the patients presented risk factors for CRAb colonization, mainly invasive devices (87.5%) and previous antibiotic therapy (77.6%). Thirty-three patients died during hospital stay (59.0%). Resistance to carbapenems was associated with a high prevalence of blaOXA-23 (51.2%) and/or blaOXA-143 (18.6%) genes. ERIC-PCR genotyping identified 10 clusters among OXA-producing CRAb. Three CRAb strains exhibited additional resistance to polymyxin B (MIC≥4µg/mL), whereas 10 CRAb strains showed tigecycline MICs>2µg/mL. CONCLUSIONS: In this study, clonally unrelated OXA-123- and OXA-143-producing A. baumannii strains in ICU patients were strongly correlated to colonization with infected patients being associated with a poor outcome.


Assuntos
Infecções por Acinetobacter/microbiologia , Acinetobacter baumannii/enzimologia , Antibacterianos/farmacologia , Infecção Hospitalar/microbiologia , beta-Lactamases/biossíntese , Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/genética , Brasil , Feminino , Genótipo , Hospitais de Ensino , Humanos , Unidades de Terapia Intensiva , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Multiplex , Estudos Prospectivos , beta-Lactamases/genética
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