RESUMO
INTRODUCTION: Combat readiness assessments through simulated tasks (STs) have been developed for the Brazilian Air Force (BAF) to establish physical employment standards. Previous research has established BAF critical combat tasks with STs developed based on the physical demands of these tasks. Before implementing these STs, the standards required of BAF personnel must be established. The aim of this study was to determine the cut-off scores for five previously established STs. METHODS: Eighty-eight cadets attended three different testing batteries in order to complete the five STs, being: Battery 1 (foot march), Battery 2 (plane crash on water and water survival skills) and Battery 3 (plane crash on land, obstacle course) with their times recorded. Cut-off scores were set at the 85th percentile of the data distribution with these values and then analysed by four subject matter experts (SMEs) using subjective criteria through criterion analysis. RESULTS: All 88 cadets were submitted to the five assessments. After analysing the performance results on the STs, the SMEs discussed and agreed on the following cut-off scores: obstacle course (3:21 min:s), foot march (31:00 min:s), plane crash on land (1:25 min:s), plane crash on water (1:12 min:s) and water survival skills (4:03 min:s). CONCLUSION: The outcomes of this research allow for the five STs to be implemented in BAF cadets and qualified BAF personnel with the established cut-off scores used to monitor the operational capability of these personnel (be it for cadet training outcomes or unit preparedness assessments) and to guide conditioning practices if personnel are below standards.
RESUMO
PURPOSE: Despite various existing surgical techniques, treatment of facial nerve palsy remains difficult. The purpose of this report is to present the cerclage sling technique using temporalis fascia to manage paralytic lagophthalmos. METHODS: A series of six patients underwent a cerclage sling technique using temporalis muscle fascia to treat paralytic lagophthalmos. The technique is presented in detail. Symptoms, palpebral fissures, and lagophthalmos were assessed pre- and postoperatively. Data were submitted for statistical analysis. RESULTS: After surgery, all patients achieved a reduction in clinical symptoms. The upper eyelids had lowered, and the inferior eyelids had elevated, reducing ocular exposure even if mild residual lagophthalmos was present. CONCLUSION: Cerclage using the temporalis muscle fascia sling technique is a safe and effective procedure to treat facial nerve paralytic lagophthalmos. A reduction in ocular exposure and lagophthalmos provides improvement in clinical symptoms and eyelid function.
Assuntos
Doenças Palpebrais , Paralisia Facial , Lagoftalmia , Humanos , Doenças Palpebrais/etiologia , Doenças Palpebrais/cirurgia , Pálpebras/cirurgia , Paralisia Facial/complicações , Paralisia Facial/cirurgia , Fáscia/transplante , MúsculosRESUMO
Sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) and sarcolemmal Na+/Ca2+ exchanger (NCX1) structures are involved in heart cell Ca2+ homeostasis. Previous studies have shown discrepancies in their function and expression in heart failure. The goal of this study was to evaluate heart function and hypertrophied muscle Ca2+-handling protein behavior under pressure overload. Twenty male Wistar rats were divided into two groups: Aortic stenosis (AoS), induced by a clip placed at the beginning of the aorta, and Control (Sham). After 18 weeks, heart function and structure were evaluated by echocardiogram. Myocardial function was analyzed by isolated papillary muscle (IPM) at basal condition and Ca2+ protein functions were evaluated after post-pause contraction and blockage with cyclopiazonic acid in IPM. Ca2+-handling protein expression was studied by western blot (WB). Echocardiogram showed that AoS caused concentric hypertrophy with enhanced ejection fraction and diastolic dysfunction inferred by dilated left atrium and increased relative wall thickness. IPM study showed developed tension was the same in both groups. AoS showed increased stiffness revealed by enhanced resting tension, and changes in Ca2+ homeostasis shown by calcium elevation and SERCA2a blockage maneuvers. WB revealed decreased NCX1, SERCA2a, and phosphorylated phospholambam (PLB) on serine-16 in AoS. AoS had left ventricular hypertrophy and diastolic dysfunction compared to Sham; this could be related to our findings regarding calcium homeostasis behavior: deficit in NCX1, SERCA2a, and phosphorylated PLB on serine-16.
Assuntos
Cálcio , Remodelação Ventricular , Animais , Cálcio/metabolismo , Homeostase , Masculino , Ratos , Ratos Wistar , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismoRESUMO
Sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) and sarcolemmal Na+/Ca2+ exchanger (NCX1) structures are involved in heart cell Ca2+ homeostasis. Previous studies have shown discrepancies in their function and expression in heart failure. The goal of this study was to evaluate heart function and hypertrophied muscle Ca2+-handling protein behavior under pressure overload. Twenty male Wistar rats were divided into two groups: Aortic stenosis (AoS), induced by a clip placed at the beginning of the aorta, and Control (Sham). After 18 weeks, heart function and structure were evaluated by echocardiogram. Myocardial function was analyzed by isolated papillary muscle (IPM) at basal condition and Ca2+ protein functions were evaluated after post-pause contraction and blockage with cyclopiazonic acid in IPM. Ca2+-handling protein expression was studied by western blot (WB). Echocardiogram showed that AoS caused concentric hypertrophy with enhanced ejection fraction and diastolic dysfunction inferred by dilated left atrium and increased relative wall thickness. IPM study showed developed tension was the same in both groups. AoS showed increased stiffness revealed by enhanced resting tension, and changes in Ca2+ homeostasis shown by calcium elevation and SERCA2a blockage maneuvers. WB revealed decreased NCX1, SERCA2a, and phosphorylated phospholambam (PLB) on serine-16 in AoS. AoS had left ventricular hypertrophy and diastolic dysfunction compared to Sham; this could be related to our findings regarding calcium homeostasis behavior: deficit in NCX1, SERCA2a, and phosphorylated PLB on serine-16.
Assuntos
Animais , Masculino , Ratos , Cálcio/metabolismo , Remodelação Ventricular , Ratos Wistar , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo , HomeostaseRESUMO
Coturniculture has increased significantly in the last decades. There are several pathogens that can affect these birds. Among the diseases, fowl typhoid stands out as a disease with a potentially great impact to the poultry industry. The objective of this the study was to evaluate the effect of doses and administration routes of live 9R vaccine on protection of Japanese quails against experimental infection with Salmonella Gallinarum (SG). Two hundred and fifty birds were used, divided into five groups: G1, oral vaccination with one dose; G2, oral vaccination with 2 doses; G3, subcutaneous vaccination with one dose; G4, subcutaneous vaccination with two doses and G5 not vaccinated. All birds from all five groups were challenged with SG at an age of 45 days. SG was quantified in the periods of one, four, seven and twelve days after the challenge. The presence of clinical signs and macroscopic lesions of the disease were observed. The groups vaccinated by subcutaneous route had a higher egg production and lower mortality rate. Birds receiving a dose of the vaccine by subcutaneous route also showed lower amount of SG in the liver and spleen seven days after the challenge.(AU)
A coturnicultura tem aumentado significativamente nas últimas décadas. Existem vários patógenos que podem afetar essas aves. Entre as doenças, o tifo aviário se destaca como uma doença de grande impacto para a indústria avícola. O objetivo deste estudo foi avaliar o efeito de doses e vias de administração da vacina viva 9R na proteção de codornas japonesas contra infecção experimental por Salmonella Gallinarum (SG). Foram utilizadas duzentos e cinquenta aves, divididas em cinco grupos: G1, vacinação oral com uma dose; G2, vacinação oral com 2 doses; G3, vacinação subcutânea com uma dose; G4, vacinação subcutânea com duas doses e G5 não vacinado. Todas as aves dos cinco grupos foram desafiadas com SG aos 45 dias de idade. A SG foi quantificada nos períodos de um, quatro, sete e doze dias após o desafio. Foi observada a presença de sinais clínicos e lesões macroscópicas da doença. Os grupos vacinados por via subcutânea apresentaram maior produção de ovos e menor taxa de mortalidade. Aves recebendo uma dose da vacina por via subcutânea também apresentaram menor quantidade de SG no fígado e baço sete dias após o desafio.(AU)
Assuntos
Animais , Salmonella/imunologia , Vacinas/administração & dosagem , Vias de Administração de Medicamentos/veterinária , Coturnix/imunologiaRESUMO
Coturniculture has increased significantly in the last decades. There are several pathogens that can affect these birds. Among the diseases, fowl typhoid stands out as a disease with a potentially great impact to the poultry industry. The objective of this the study was to evaluate the effect of doses and administration routes of live 9R vaccine on protection of Japanese quails against experimental infection with Salmonella Gallinarum (SG). Two hundred and fifty birds were used, divided into five groups: G1, oral vaccination with one dose; G2, oral vaccination with 2 doses; G3, subcutaneous vaccination with one dose; G4, subcutaneous vaccination with two doses and G5 not vaccinated. All birds from all five groups were challenged with SG at an age of 45 days. SG was quantified in the periods of one, four, seven and twelve days after the challenge. The presence of clinical signs and macroscopic lesions of the disease were observed. The groups vaccinated by subcutaneous route had a higher egg production and lower mortality rate. Birds receiving a dose of the vaccine by subcutaneous route also showed lower amount of SG in the liver and spleen seven days after the challenge.(AU)
A coturnicultura tem aumentado significativamente nas últimas décadas. Existem vários patógenos que podem afetar essas aves. Entre as doenças, o tifo aviário se destaca como uma doença de grande impacto para a indústria avícola. O objetivo deste estudo foi avaliar o efeito de doses e vias de administração da vacina viva 9R na proteção de codornas japonesas contra infecção experimental por Salmonella Gallinarum (SG). Foram utilizadas duzentos e cinquenta aves, divididas em cinco grupos: G1, vacinação oral com uma dose; G2, vacinação oral com 2 doses; G3, vacinação subcutânea com uma dose; G4, vacinação subcutânea com duas doses e G5 não vacinado. Todas as aves dos cinco grupos foram desafiadas com SG aos 45 dias de idade. A SG foi quantificada nos períodos de um, quatro, sete e doze dias após o desafio. Foi observada a presença de sinais clínicos e lesões macroscópicas da doença. Os grupos vacinados por via subcutânea apresentaram maior produção de ovos e menor taxa de mortalidade. Aves recebendo uma dose da vacina por via subcutânea também apresentaram menor quantidade de SG no fígado e baço sete dias após o desafio.(AU)
Assuntos
Animais , Salmonella/imunologia , Vacinas/administração & dosagem , Vias de Administração de Medicamentos/veterinária , Coturnix/imunologiaRESUMO
Conhecer as características das adoções de animais de canis municipais pode contribuir para que ações educativas de guarda responsável sejam mais precisas e direcionadas. Este trabalho teve como objetivo descrever as adoções de cães e gatos do canil municipal de Botucatu/SP, a fim de investigar a possível associação entre adoção e renda, além do sexo dos adotantes, para direcionar de uma melhor maneira ações educativas de guarda responsável. As informações foram coletadas de 998 termos de adoção. A partir dos endereços das adoções, identificaram-se os bairros que mais adotaram e suas rendas médias. As informações foram armazenadas em banco de dados criado na planilha eletrônica do Excel. As distribuições frequenciais foram processadas por meio do software SPSS. Observou-se que não foi estabelecida associação entre o número de adoções e a renda. Em relação ao gênero dos adotantes, houve predominância do sexo feminino. O conhecimento destas características auxiliará na elaboração de ações educativas de guarda responsável, contribuindo para minimizar o número de animais que oferecem risco para a saúde pública e que precisam ser acolhidos pelos canis.
RESUMO
Conhecer as características das adoções de animais de canis municipais pode contribuir para que ações educativas de guarda responsável sejam mais precisas e direcionadas. Este trabalho teve como objetivo descrever as adoções de cães e gatos do canil municipal de Botucatu/SP, a fim de investigar a possível associação entre adoção e renda, além do sexo dos adotantes, para direcionar de uma melhor maneira ações educativas de guarda responsável. As informações foram coletadas de 998 termos de adoção. A partir dos endereços das adoções, identificaram-se os bairros que mais adotaram e suas rendas médias. As informações foram armazenadas em banco de dados criado na planilha eletrônica do Excel. As distribuições frequenciais foram processadas por meio do software SPSS. Observou-se que não foi estabelecida associação entre o número de adoções e a renda. Em relação ao gênero dos adotantes, houve predominância do sexo feminino. O conhecimento destas características auxiliará na elaboração de ações educativas de guarda responsável, contribuindo para minimizar o número de animais que oferecem risco para a saúde pública e que precisam ser acolhidos pelos canis.(AU)
Knowing the characteristics of the adoption of municipal shelter animals can contribute in making educational actions of responsible pet ownership more precise and directed. This study aimed to describe the adoption of dogs and cats from the municipal animal shelter of Botucatu/SP, in order to investigate the possible association between adoption and income, and also the gender of the adopters, for the purpose of better directing educational actions of responsible pet ownership. The information was collected from 998 terms of adoption. From the addresses of the adoptions, it was possible to identify the neighborhoods that had the most adoptions and their average income. The information was stored in a database created in Excel. Frequency distributions were assessed by SPSS software. It was observed that there was no association between number of adoptions and income. Regarding gender, there was a predominance of female adopters. Knowing these characteristics will help create educational actions of responsible pet ownership, contributing to minimize the number of animals that pose a risk to public health and that need to be rescued by animal shelters.(AU)
Assuntos
Animais , Gatos , Cães , Adoção , Abrigo para Animais , Animais de EstimaçãoRESUMO
Conhecer as características das adoções de animais de canis municipais pode contribuir para que ações educativas de guarda responsável sejam mais precisas e direcionadas. Este trabalho teve como objetivo descrever as adoções de cães e gatos do canil municipal de Botucatu/SP, a fim de investigar a possível associação entre adoção e renda, além do sexo dos adotantes, para direcionar de uma melhor maneira ações educativas de guarda responsável. As informações foram coletadas de 998 termos de adoção. A partir dos endereços das adoções, identificaram-se os bairros que mais adotaram e suas rendas médias. As informações foram armazenadas em banco de dados criado na planilha eletrônica do Excel. As distribuições frequenciais foram processadas por meio do software SPSS. Observou-se que não foi estabelecida associação entre o número de adoções e a renda. Em relação ao gênero dos adotantes, houve predominância do sexo feminino. O conhecimento destas características auxiliará na elaboração de ações educativas de guarda responsável, contribuindo para minimizar o número de animais que oferecem risco para a saúde pública e que precisam ser acolhidos pelos canis.
Knowing the characteristics of the adoption of municipal shelter animals can contribute in making educational actions of responsible pet ownership more precise and directed. This study aimed to describe the adoption of dogs and cats from the municipal animal shelter of Botucatu/SP, in order to investigate the possible association between adoption and income, and also the gender of the adopters, for the purpose of better directing educational actions of responsible pet ownership. The information was collected from 998 terms of adoption. From the addresses of the adoptions, it was possible to identify the neighborhoods that had the most adoptions and their average income. The information was stored in a database created in Excel. Frequency distributions were assessed by SPSS software. It was observed that there was no association between number of adoptions and income. Regarding gender, there was a predominance of female adopters. Knowing these characteristics will help create educational actions of responsible pet ownership, contributing to minimize the number of animals that pose a risk to public health and that need to be rescued by animal shelters.
Assuntos
Animais , Gatos , Cães , Abrigo para Animais , Adoção , Animais de EstimaçãoRESUMO
Obesity is often associated with changes in cardiac function; however, the mechanisms responsible for functional abnormalities have not yet been fully clarified. Considering the lack of information regarding high-saturated-fat diet-induced obesity, heart function, and the proteins involved in myocardial calcium (Ca2+) handling, the aim of this study was to test the hypothesis that this dietary model of obesity leads to cardiac dysfunction resulting from alterations in the regulatory proteins of intracellular Ca2+ homeostasis. Male Wistar rats were distributed into two groups: control (C, n=18; standard diet) and obese (Ob, n=19; high-saturated-fat diet), which were fed for 33 weeks. Cardiac structure and function were evaluated using echocardiographic and isolated papillary muscle analyses. Myocardial protein expressions of sarcoplasmic reticulum Ca2+-ATPase, phospholamban (PLB), PLB serine-16 phosphorylation, PLB threonine-17 phosphorylation, ryanodine receptor, calsequestrin, Na+/Ca2+ exchanger, and L-type Ca2+ channel were assessed by western blot. Obese rats presented 104% increase in the adiposity index (C: 4.5±1.4 vs Ob: 9.2±1.5%) and obesity-related comorbidities compared to control rats. The left atrium diameter (C: 5.0±0.4 vs Ob: 5.5±0.5 mm) and posterior wall shortening velocity (C: 36.7±3.4 vs Ob: 41.8±3.8 mm/s) were higher in the obese group than in the control. The papillary muscle function was similar between the groups at baseline and after inotropic and lusitropic maneuvers. Obesity did not lead to changes in myocardial Ca2+ handling proteins expression. In conclusion, the hypothesis was not confirmed, since the high-saturated-fat diet-induced obese rats did not present cardiac dysfunction or impaired intracellular Ca2+ handling proteins.
Assuntos
Cálcio/fisiologia , Dieta Hiperlipídica/efeitos adversos , Coração/fisiopatologia , Obesidade/fisiopatologia , Trocador de Sódio e Cálcio/fisiologia , Animais , Pressão Sanguínea/fisiologia , Modelos Animais de Doenças , Ecocardiografia , Masculino , Ratos , Ratos WistarRESUMO
Obesity is often associated with changes in cardiac function; however, the mechanisms responsible for functional abnormalities have not yet been fully clarified. Considering the lack of information regarding high-saturated-fat diet-induced obesity, heart function, and the proteins involved in myocardial calcium (Ca2+) handling, the aim of this study was to test the hypothesis that this dietary model of obesity leads to cardiac dysfunction resulting from alterations in the regulatory proteins of intracellular Ca2+ homeostasis. Male Wistar rats were distributed into two groups: control (C, n=18; standard diet) and obese (Ob, n=19; high-saturated-fat diet), which were fed for 33 weeks. Cardiac structure and function were evaluated using echocardiographic and isolated papillary muscle analyses. Myocardial protein expressions of sarcoplasmic reticulum Ca2+-ATPase, phospholamban (PLB), PLB serine-16 phosphorylation, PLB threonine-17 phosphorylation, ryanodine receptor, calsequestrin, Na+/Ca2+ exchanger, and L-type Ca2+ channel were assessed by western blot. Obese rats presented 104% increase in the adiposity index (C: 4.5±1.4 vs Ob: 9.2±1.5%) and obesity-related comorbidities compared to control rats. The left atrium diameter (C: 5.0±0.4 vs Ob: 5.5±0.5 mm) and posterior wall shortening velocity (C: 36.7±3.4 vs Ob: 41.8±3.8 mm/s) were higher in the obese group than in the control. The papillary muscle function was similar between the groups at baseline and after inotropic and lusitropic maneuvers. Obesity did not lead to changes in myocardial Ca2+ handling proteins expression. In conclusion, the hypothesis was not confirmed, since the high-saturated-fat diet-induced obese rats did not present cardiac dysfunction or impaired intracellular Ca2+ handling proteins.
Assuntos
Animais , Masculino , Ratos , Cálcio/fisiologia , Trocador de Sódio e Cálcio/fisiologia , Dieta Hiperlipídica/efeitos adversos , Coração/fisiopatologia , Obesidade/fisiopatologia , Pressão Sanguínea/fisiologia , Ecocardiografia , Ratos Wistar , Modelos Animais de DoençasRESUMO
BACKGROUND: Hepatic circulatory disturbances have been associated with obesity and fatty liver in humans. In the veterinary literature, however, there is limited information regarding the effects of different body condition scores (BCS) on liver hemodynamic indices in dogs. OBJECTIVES: To investigate the influence of BCS on liver hemodynamic indices. ANIMALS: Fifty-three client-owned dogs of various breeds were included. METHODS: Prospective observational study. Dogs were divided into 3 BCS groups using a 5-point scale: G1 - 12 ideal dogs, G2 - 21 overweight dogs, G3 - 20 obese dogs. Mean portal velocity (MPV), portal blood flow volume (PBFV), portal congestion index (PCI), hepatic artery resistivity index (HARI), and hepatic vein (HV) spectral wave were obtained by pulsed Doppler sonography. Alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and alanine aminotransferase (ALT) activities were determined. Liver enzymes activities and liver hemodynamic indices were compared among groups. RESULTS: Obese dogs had lower MPV, higher percentage of abnormal hepatic vein spectral wave and higher median ALP activity than did ideal dogs (P < 0.05). Overweight and obese dogs had lower PBFV than ideal dogs (P < 0.01). Overweight dogs had higher median GGT activity than ideal dogs (P < 0.05). No difference was observed for PCI, HARI and median ALT activity among the groups. CONCLUSIONS AND CLINICAL IMPORTANCE: Obesity was associated with changes in portal vein indices and in HV spectral wave. These changes were accompanied by significant differences in some liver enzymes activities and could be a sign of early liver disease.
Assuntos
Doenças do Cão/fisiopatologia , Fígado/irrigação sanguínea , Obesidade/veterinária , Sobrepeso/veterinária , Animais , Doenças do Cão/diagnóstico por imagem , Cães , Feminino , Hemodinâmica/fisiologia , Fígado/diagnóstico por imagem , Circulação Hepática/fisiologia , Masculino , Obesidade/diagnóstico por imagem , Obesidade/fisiopatologia , Sobrepeso/diagnóstico por imagem , Sobrepeso/fisiopatologia , Ultrassonografia Doppler/veterináriaRESUMO
Cardiac remodeling is defined as changes in shape and function of the heart in response to aggression (pressure overload). The sarcoplasmic reticulum calcium ATPase cardiac isoform 2a (SERCA2a) is a known factor that influences function. A wide spectrum of studies report a decrease in SERCA2a in heart failure, but none evaluate it's the role in early isolated diastolic dysfunction in supravalvular aortic stenosis (AoS). Our hypothesis was that SERCA2a participates in such dysfunction. Thirty-day-old male Wistar rats (60-80 g) were divided into AoS and Sham groups, which were submitted to surgery with or without aorta clipping, respectively. After 6 weeks, the animals were submitted to echocardiogram and functional analysis by isolated papillary muscle (IPM) in basal condition, hypoxia, and SERCA2a blockage with cyclopiazonic acid at calcium concentrations of 0.5, 1.5, and 2.5 mM. Western-blot analyses were used for SERCA2a and phospholamban detection. Data analysis was carried out with Student's t-test and ANOVA. AoS enhanced left atrium and E and A wave ratio, with preserved ejection fraction. Basal condition in IPM showed similar increases in developed tension (DT) and resting tension (RT) in AoS, and hypoxia was similar between groups. After cyclopiazonic acid blockage, final DT was equally decreased and RT was similar between groups, but the speed of relaxation was decreased in the AoS group. Western-blot was uniform in all evaluations. The hypothesis was confirmed, since functional parameters regarding SERCA2a were changed in the AoS group.
Assuntos
Estenose Aórtica Supravalvular/complicações , Hipertrofia Ventricular Esquerda/fisiopatologia , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/fisiologia , Disfunção Ventricular Esquerda/fisiopatologia , Animais , Estenose Aórtica Supravalvular/metabolismo , Proteínas de Ligação ao Cálcio/análise , Colágeno/análise , Diástole/fisiologia , Modelos Animais de Doenças , Ecocardiografia , Ventrículos do Coração/patologia , Ventrículos do Coração/fisiopatologia , Hipertrofia Ventricular Esquerda/etiologia , Hipertrofia Ventricular Esquerda/metabolismo , Hipóxia/metabolismo , Hipóxia/fisiopatologia , Indóis , Masculino , Contração Miocárdica/fisiologia , Ratos Wistar , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/análise , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo , Fatores de Tempo , Disfunção Ventricular Esquerda/etiologia , Disfunção Ventricular Esquerda/metabolismo , Remodelação Ventricular/fisiologiaRESUMO
Among the different materials for bladder augmentation, porcine intestinal submucosa (SIS) is the most widely investigated and stands out for its ability as a cell scaffold. In this context, the ultrasound examination allows the detection of changes from the surgical procedure, enabling the early verification of potential complications and evaluation of patient outcomes. The aim of this paper is to describe the main sonographic findings in dogs submitted to cystoplasty using acellular SIS and seeded with homologous smooth muscle cells at 30 (M30) and 60 (M60) days postoperatively. Sonographic changes included irregularities and thickening of bladder wall especially at M30. Additionally, were visualized urinary sediment and uroliths in animals submitted to acellular SIS cistoplasty. Abdominal ultrasonography was useful in the postoperative evaluation of animals undergoing cystoplasty with acellular or seeded SIS.(AU)
Entre os diferentes materiais utilizados para a reparação e o aumento da bexiga, a submucosa intestinal suína (SIS) é o mais amplamente investigado e se destaca por sua habilidade como um arcabouço celular. Nesse contexto, o exame ultrassonográfico possibilita a avaliação pós-operatória, bem como a detecção precoce de potenciais complicações. O objetivo deste artigo é descrever os principais achados ultrassonográficos em cães submetidos à cistoplastia utilizando a SIS acelular e semeada com células musculares lisas homólogas aos 30 (M30) e 60 (M60) dias de pós-operatório. As alterações ultrassonográficas pós-operatórias incluíram irregularidades e espessamento da parede da bexiga, especialmente aos M30. Além disso, foram visualizados sedimento urinário e urólitos em animais submetidos à cistoplastia utilizando a SIS acelular. A ecografia abdominal foi útil na avaliação pós-operatória dos animais submetidos à cistoplastia utilizando a SIS acelular e semeada com células musculares lisas homólogas.(AU)
Assuntos
Animais , Cães , Terapia Baseada em Transplante de Células e Tecidos/veterinária , Procedimentos de Cirurgia Plástica/veterinária , Bexiga Urinária/cirurgia , Ultrassonografia/veterináriaRESUMO
Among the different materials for bladder augmentation, porcine intestinal submucosa (SIS) is the most widely investigated and stands out for its ability as a cell scaffold. In this context, the ultrasound examination allows the detection of changes from the surgical procedure, enabling the early verification of potential complications and evaluation of patient outcomes. The aim of this paper is to describe the main sonographic findings in dogs submitted to cystoplasty using acellular SIS and seeded with homologous smooth muscle cells at 30 (M30) and 60 (M60) days postoperatively. Sonographic changes included irregularities and thickening of bladder wall especially at M30. Additionally, were visualized urinary sediment and uroliths in animals submitted to acellular SIS cistoplasty. Abdominal ultrasonography was useful in the postoperative evaluation of animals undergoing cystoplasty with acellular or seeded SIS.(AU)
Entre os diferentes materiais utilizados para a reparação e o aumento da bexiga, a submucosa intestinal suína (SIS) é o mais amplamente investigado e se destaca por sua habilidade como um arcabouço celular. Nesse contexto, o exame ultrassonográfico possibilita a avaliação pós-operatória, bem como a detecção precoce de potenciais complicações. O objetivo deste artigo é descrever os principais achados ultrassonográficos em cães submetidos à cistoplastia utilizando a SIS acelular e semeada com células musculares lisas homólogas aos 30 (M30) e 60 (M60) dias de pós-operatório. As alterações ultrassonográficas pós-operatórias incluíram irregularidades e espessamento da parede da bexiga, especialmente aos M30. Além disso, foram visualizados sedimento urinário e urólitos em animais submetidos à cistoplastia utilizando a SIS acelular. A ecografia abdominal foi útil na avaliação pós-operatória dos animais submetidos à cistoplastia utilizando a SIS acelular e semeada com células musculares lisas homólogas.(AU)
Assuntos
Animais , Cães , Cães , Bexiga Urinária/cirurgia , Procedimentos de Cirurgia Plástica/veterinária , Terapia Baseada em Transplante de Células e Tecidos/veterinária , Ultrassonografia/veterináriaRESUMO
In experimental studies, several parameters, such as body weight, body mass index, adiposity index, and dual-energy X-ray absorptiometry, have commonly been used to demonstrate increased adiposity and investigate the mechanisms underlying obesity and sedentary lifestyles. However, these investigations have not classified the degree of adiposity nor defined adiposity categories for rats, such as normal, overweight, and obese. The aim of the study was to characterize the degree of adiposity in rats fed a high-fat diet using cluster analysis and to create adiposity intervals in an experimental model of obesity. Thirty-day-old male Wistar rats were fed a normal (n=41) or a high-fat (n=43) diet for 15 weeks. Obesity was defined based on the adiposity index; and the degree of adiposity was evaluated using cluster analysis. Cluster analysis allowed the rats to be classified into two groups (overweight and obese). The obese group displayed significantly higher total body fat and a higher adiposity index compared with those of the overweight group. No differences in systolic blood pressure or nonesterified fatty acid, glucose, total cholesterol, or triglyceride levels were observed between the obese and overweight groups. The adiposity index of the obese group was positively correlated with final body weight, total body fat, and leptin levels. Despite the classification of sedentary rats into overweight and obese groups, it was not possible to identify differences in the comorbidities between the two groups.
Assuntos
Adiposidade/fisiologia , Modelos Animais de Doenças , Obesidade/classificação , Comportamento Sedentário , Animais , Glicemia/análise , Pressão Sanguínea , Peso Corporal , Colesterol/sangue , Análise por Conglomerados , Dieta Hiperlipídica , Ácidos Graxos não Esterificados/sangue , Insulina/sangue , Leptina/sangue , Masculino , Ratos Wistar , Índice de Gravidade de Doença , Fatores de Tempo , Triglicerídeos/sangueRESUMO
Objetivou-se com este trabalho determinar o poder dióptrico da lente intraocular (LIO) em miniporco e as dimensões do bulbo do olho. Foram utilizados 17 miniporcos, sadios, adultos, machos e fêmeas, com peso médio de 70kg. Em todos os olhos foram realizadas a ultrassonografia modo A, a ceratometria e a medida da distância limbo a limbo. O cálculo do poder dióptrico da LIO foi obtido utilizando-se as fórmulas Haigis, Hoffer Q, SRK/T, Holladay I e Holladay II e o software Holladay IOL Consultant(r). Na comparação entre o sexo e a lateralidade do olho, não houve diferença nas variáveis biométricas e poder da LIO. A aplicação das fórmulas (Haigis, Holladay II, Holladay I, SRK/T e Hoffer Q) possibilitou o cálculo do poder da LIO. A Holladay II, fórmula que melhor individualiza o bulbo do olho do miniporco, estima valor dióptrico ao redor de 41 D. Os miniporcos têm potencial como modelo experimental em oftalmologia, relacionado ao seu menor porte e à facilidade no manejo, especialmente em experimentos de longa duração.
The aim of this study was to determine the refractive power of intraocular lens (IOL) of mini pigs and the dimensions of the eyeball. A total of 17 (34 eyes) healthy, adult, males and female animals, with average weight of 70kg were used. For every eye, A-mode ultrasound, keratometry and the measurement of limbo-to-limbo distance were conducted, all variables for calculating the refractive power of the IOL. The value was obtained using different formulas and Holladay IOL Consultant(r) Software. Additionally, the ocular measurements were compared per sex, laterality of the eye and the different formulas used in this study (Haigis, Hoffer Q, SRK / T, Holladay I and Holladay II). In the comparison between sex and laterality of the eye, there was no difference in biometric variables and power of the IOL. The application of the employed formulas (Haigis, Holladay II, Holladay I, SRK / T and Hoffer Q) allowed the IOL power calculation for this specie, and the observed value ranged between 39.58±2.15 and 46.60±2.81 diopters. Mini pigs play an important and growing role as an experimental model for study and practice of ophthalmic procedures, specially related to their smaller size and easy management in long-term experiments.
Assuntos
Animais , Experimentação Animal , Oftalmologia , Porco Miniatura/anatomia & histologia , Biometria , Lentes Intraoculares/veterinária , Modelos AnimaisRESUMO
Objetivou-se com este trabalho determinar o poder dióptrico da lente intraocular (LIO) em miniporco e as dimensões do bulbo do olho. Foram utilizados 17 miniporcos, sadios, adultos, machos e fêmeas, com peso médio de 70kg. Em todos os olhos foram realizadas a ultrassonografia modo A, a ceratometria e a medida da distância limbo a limbo. O cálculo do poder dióptrico da LIO foi obtido utilizando-se as fórmulas Haigis, Hoffer Q, SRK/T, Holladay I e Holladay II e o software Holladay IOL Consultant(r). Na comparação entre o sexo e a lateralidade do olho, não houve diferença nas variáveis biométricas e poder da LIO. A aplicação das fórmulas (Haigis, Holladay II, Holladay I, SRK/T e Hoffer Q) possibilitou o cálculo do poder da LIO. A Holladay II, fórmula que melhor individualiza o bulbo do olho do miniporco, estima valor dióptrico ao redor de 41 D. Os miniporcos têm potencial como modelo experimental em oftalmologia, relacionado ao seu menor porte e à facilidade no manejo, especialmente em experimentos de longa duração.(AU)
The aim of this study was to determine the refractive power of intraocular lens (IOL) of mini pigs and the dimensions of the eyeball. A total of 17 (34 eyes) healthy, adult, males and female animals, with average weight of 70kg were used. For every eye, A-mode ultrasound, keratometry and the measurement of limbo-to-limbo distance were conducted, all variables for calculating the refractive power of the IOL. The value was obtained using different formulas and Holladay IOL Consultant(r) Software. Additionally, the ocular measurements were compared per sex, laterality of the eye and the different formulas used in this study (Haigis, Hoffer Q, SRK / T, Holladay I and Holladay II). In the comparison between sex and laterality of the eye, there was no difference in biometric variables and power of the IOL. The application of the employed formulas (Haigis, Holladay II, Holladay I, SRK / T and Hoffer Q) allowed the IOL power calculation for this specie, and the observed value ranged between 39.58±2.15 and 46.60±2.81 diopters. Mini pigs play an important and growing role as an experimental model for study and practice of ophthalmic procedures, specially related to their smaller size and easy management in long-term experiments.(AU)
Assuntos
Animais , Porco Miniatura/anatomia & histologia , Experimentação Animal , Oftalmologia , Biometria , Modelos Animais , Lentes Intraoculares/veterináriaRESUMO
PURPOSE: Communication barriers during the process of cancer diagnosis can adversely affect how patients understand their health, and understanding patient's perceptions and expectations can favor adherence to professional recommendations and their prognosis. This study aimed to describe the sociodemographic characteristics, perceptions, expectations, and psychological symptoms of patients during the process of oral cancer diagnosis. METHODS: Patients were assessed at two time points: pre-biopsy (T0) and post-diagnosis (T1). At T0, 49 patients answered a sociodemographic, perceived social support, lifestyle questionnaire and inventories (anxiety-State-Trait Anxiety Inventory (STAI-S/T), depression-Beck Depression Inventory (BDI), risk drinkers-Alcohol Use Disorders Identification Test (AUDIT)). They also were interviewed concerning their perceptions, feelings, and expectations. At T1, the 29 patients diagnosed with oral cancer were reassessed for anxiety (STAI-E) and depression symptoms. An interview investigated the same aspects at T0. RESULTS: Patient mean age was 59 years old (±13.7) and 73.5 % were men. At T0, depression symptoms were more frequent among cancer patients (83.3 %) than those without the disease (p < 0.05), with a prevalence of 36.7 %. Patients presented anxiety (40.8 %) and alcohol abuse behavior (32.6 %). Associations between characteristics and categories at T0 showed that patients with lower income and living with a partner reported negative feelings (p < 0.05) more frequently, together with negative expectations concerning the diagnosis among older patients (p < 0.05) and those with depressive symptoms (p < 0.05). At T1, negative feelings were more frequently reported among patients with anxiety (p < 0.01) and depression symptoms (p < 0.05). CONCLUSION: Professional support and care regarding the psychological aspects and characteristics of patients is required during the diagnosis process in order to promote patient adhesion and favorable prognosis.
Assuntos
Neoplasias Bucais/diagnóstico , Neoplasias Bucais/psicologia , Adulto , Estudos Transversais , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Percepção , Prognóstico , Apoio Social , Inquéritos e Questionários , Adulto JovemRESUMO
In experimental studies, several parameters, such as body weight, body mass index, adiposity index, and dual-energy X-ray absorptiometry, have commonly been used to demonstrate increased adiposity and investigate the mechanisms underlying obesity and sedentary lifestyles. However, these investigations have not classified the degree of adiposity nor defined adiposity categories for rats, such as normal, overweight, and obese. The aim of the study was to characterize the degree of adiposity in rats fed a high-fat diet using cluster analysis and to create adiposity intervals in an experimental model of obesity. Thirty-day-old male Wistar rats were fed a normal (n=41) or a high-fat (n=43) diet for 15 weeks. Obesity was defined based on the adiposity index; and the degree of adiposity was evaluated using cluster analysis. Cluster analysis allowed the rats to be classified into two groups (overweight and obese). The obese group displayed significantly higher total body fat and a higher adiposity index compared with those of the overweight group. No differences in systolic blood pressure or nonesterified fatty acid, glucose, total cholesterol, or triglyceride levels were observed between the obese and overweight groups. The adiposity index of the obese group was positively correlated with final body weight, total body fat, and leptin levels. Despite the classification of sedentary rats into overweight and obese groups, it was not possible to identify differences in the comorbidities between the two groups.