RESUMO
This study evaluated the biocidal activity of aqueous fruit extracts from Ilex paraguariensis progenies at different ripening stages in two ecotoxicity assays using Pomacea canaliculata juveniles and Danio rerio larvae. Mutagenicity was verified by the Salmonella/microsome assay (TA 98 and TA 100) in the presence and absence of hepatic metabolism in vitro. The results showed that the snails were more sensitive than the larvae to the P.1 progeny extracts in all three evaluated ripening stages. P.3 progeny extracts were the most toxic to Danio rerio larvae after 96 hours of exposure. The toxicity results indicate that the progenies' selection influenced the metabolic contents present in the fruits in all ripening stages over time, indicating high molluscicidal activity for the P.1 progeny. In the Salmonella/microsome assays, the semi-ripe and ripe extracts from progenies P.1 and P.3 showed mainly base pair substitution mutation in assays with metabolism, but at higher doses than the toxic concentrations detected for Danio rerio or Pomacea canaliculata. Therefore, the toxicological investigation of the progenies' extracts can be interesting, given the selection of plant materials influenced the response of the bioassays.
Assuntos
Ilex paraguariensis , Agentes de Controle Biológico , Frutas , Extratos Vegetais/toxicidadeRESUMO
Neuropathic pain, depression, and anxiety are common comorbidities in diabetic patients, whose pathophysiology involves hyperglycemia-induced increased oxidative stress. Bixin (BIX), an apocarotenoid extracted from the seeds of Bixa orellana, has been used in traditional medicine to treat diabetes and has been recognized by its antioxidant profile. We aimed to investigate the effect of the BIX over the mechanical allodynia, depressive, and anxious-like behaviors associated with experimental diabetes, along with its involved mechanisms. Streptozotocin-induced diabetic rats were treated for 17 days (starting 14 days after diabetes induction) with the corresponding vehicle, BIX (10, 30 or 90 mg/kg; p.o), or INS (6 IU; s.c.). Mechanical allodynia, depressive, and anxious-like behavior were assessed by electronic Von Frey, forced swimming, and elevated plus-maze tests, respectively. Locomotor activity was assessed by the open field test. Blood glycated hemoglobin (HbA1) and the levels of lipid peroxidation (LPO) and reduced glutathione (GSH) were evaluated on the hippocampus, pre-frontal cortex, lumbar spinal cord, and sciatic nerve. Diabetic animals developed mechanical allodynia, depressive and anxious-like behavior, increased plasma HbA1, increased LPO, and decreased GSH levels in tissues analyzed. Repeated BIX-treatment (at all tested doses) significantly attenuated mechanical allodynia, the depressive (30 and 90 mg/kg) and, anxious-like behaviors (all doses) in diabetic rats, without changing the locomotor performance. BIX (at all tested doses) restored the oxidative parameters in tissues analyzed and reduced the plasma HbA1. Thereby, bixin may represent an alternative for the treatment of comorbidities associated with diabetes, counteracting oxidative stress and plasma HbA1.
Assuntos
Carotenoides/farmacologia , Hiperalgesia/tratamento farmacológico , Animais , Antioxidantes/farmacologia , Ansiedade/tratamento farmacológico , Carotenoides/metabolismo , Depressão/tratamento farmacológico , Diabetes Mellitus Experimental/fisiopatologia , Modelos Animais de Doenças , Glutationa/farmacologia , Hemoglobinas Glicadas/efeitos dos fármacos , Hemoglobinas Glicadas/metabolismo , Hipocampo/metabolismo , Hiperalgesia/metabolismo , Hiperglicemia , Peroxidação de Lipídeos , Masculino , Neuralgia/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/fisiologia , Ratos , Ratos Wistar , Nervo Isquiático/metabolismo , Estreptozocina/farmacologiaRESUMO
Abstract Our study aimed to evaluate the chemical composition of the essential oil from the leaves of Eugenia pyriformis Cambess., belonging to the Myrtaceae family and native to the Brazilian Atlantic forest. The volatile compounds in the essential oil were extracted by hydrodistillation and analyzed using GC-MS; 36 compounds accounted for 78.80% of the total oil content. The major compounds were β-caryophyllene, bicyclogermacrene, globulol, and (δ-cadinene. We evaluated their antimicrobial potential of the essential oil and toxicity to Artemia salina. The antimicrobial activity of the essential oil was evaluated against 12 microorganisms using the broth microdilution method. Our results showed moderate inhibition of Staphylococcus aureus and methicillin-resistant S. aureus (MIC, 250 and 125 μg.mL-1, respectively) and toxicity to A. salina (LC50, 125.64 μg.mL-1). Our results establish the biological activity of the essential oil obtained from E. pyriformis.
Assuntos
Óleos Voláteis , Myrtaceae/química , Resistência Microbiana a Medicamentos , Anti-InfecciososRESUMO
Bixin is the main natural apocarotenoid extracted from the seeds of Bixa orellana, widely used as a cosmetic and textile colorant. Despite the description of several pharmacological properties of B. orellana extracts, little has been studied regarding the pharmacological properties of bixin. Then we aimed to investigate the potential anti-inflammatory and antinociceptive effect of bixin in preclinical models of inflammation and acute pain. The anti-inflammatory activity of bixin (15 or 30 mg/kg, orally) was determined using carrageenan-induced paw edema and the myeloperoxidase (MPO) activity in male Wistar rats. The antinociceptive effect of bixin was assessed in the formalin and hot plate tests in rats (at same doses) and in the acetic acid-induced writhing test in Swiss albino male mice (at doses of 27 or 53 mg/kg). General locomotor activity was evaluated in the open field test. Only the higher dose of bixin significantly decreased the carrageenan-induced paw edema and the MPO activity and increased the latency time in the hot plate. Both doses of bixin significantly reduced the number of flinches in both phases of the formalin test and the number of acetic acid-induced writhings without changing the locomotor performance in the open field test. This study validates the use of bixin as an anti-inflammatory trough mechanism related to the reduction of neutrophil migration. Furthermore, this is the first report showing the antinociceptive property of bixin, which does not appear to be related to the sedative effect. Further studies are necessary to characterize the mechanisms involved in these effects.
Assuntos
Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Bixaceae/química , Carotenoides/farmacologia , Edema/tratamento farmacológico , Ácido Acético/efeitos adversos , Analgésicos/química , Animais , Anti-Inflamatórios/química , Carotenoides/química , Carragenina/efeitos adversos , Modelos Animais de Doenças , Inflamação/tratamento farmacológico , Masculino , Camundongos , Medição da Dor , Ratos , Ratos WistarRESUMO
PURPOSE: To evaluate the core (First Contact, Longitudinality, Comprehensiveness, and Coordination of Services) and derivative (Family Orientation, Community Orientation) attributes of primary health care (PHC) from the perspective of users with and without disabilities. METHODS: Observational, cross-sectional study using the Primary Care Assessment Tool (PCAT) with users with and without disabilities of five basic health units (BHU) in a municipality where 55% of the population depends on the Brazilian Unified Health System (SUS). Scores were assigned to the responses given to each of the PHC attributes evaluated. RESULTS: Study participants were 93 (67 physically disabled and 26 without disabilities) PHC users. No statistically significant differences were observed for any attribute on the comparison between the populations. For both groups, the attributes Degree of Affiliation, First Contact - Utilization, Longitudinality, and Coordination of Services - Information System received satisfactory (above cutoff) scores, whereas the attributes First Contact - Accessibility, Coordination of Services - Care Integration, Comprehensiveness, Family Orientation, and Community Orientation received unsatisfactory (below cutoff) scores. Users reported that the health teams are able to satisfactorily identify mobility issues, but there are failures in the recognition of problems of hearing, voice/speech and vision, and in the orientation of services available and services provided. CONCLUSION: Users with and without disabilities evaluated the health care received similarly, indicating fragilities on the recognition of specific demands. Structural and work process changes should be conducted to ensure Accessibility, Comprehensiveness, and Family and Community Orientation, and thus increase the quality of PHC.
OBJETIVO: Avaliar atributos essenciais (acesso de primeiro contato, longitudinalidade, integralidade e coordenação da atenção) e derivados (orientação familiar e comunitária) da Atenção Primária em Saúde (APS) na percepção de usuários com e sem deficiência. MÉTODO: Estudo transversal observacional utilizando o questionário Primary Care Assesment Tool em usuários autodeclarados com e sem deficiência de cinco unidades básicas de saúde de um município no qual 55% da população depende do SUS. Foram atribuídos escores para as respostas dadas a cada atributo de atenção primária à saúde avaliado. RESULTADOS: Participaram 93 usuários (67 com e 26 sem deficiência). Não houve diferença estatisticamente significante para nenhum dos atributos na comparação entre pessoas com e sem deficiência. Para o grupo como um todo, obtiveram-se escores acima do critério de corte: Grau de afiliação, Acesso de primeiro contato (utilização), Longitudinalidade e Coordenação da atenção (sistema de informação). Receberam pior avaliação: Acesso de primeiro contato (acessibilidade), Coordenação da atenção (integralidade do cuidado), Integralidade (serviços disponíveis e prestados) e Orientação familiar e comunitária. Os usuários reconhecem que as equipes identificam problemas de locomoção e movimentação, mas que existem falhas no reconhecimento de problemas para ouvir, falar e enxergar; e na orientação das pessoas que necessitam de auxílio e das que prestam cuidados. CONCLUSÃO: Os usuários com e sem deficiência avaliaram de forma semelhante a atenção que recebem e indicaram fragilidades no reconhecimento de demandas específicas. Há necessidade de mudanças (estruturais e do processo de trabalho) para assegurar acessibilidade, integralidade do cuidado e orientação familiar e comunitária e, assim, melhor qualificar a APS.
Assuntos
Pessoas com Deficiência , Acessibilidade aos Serviços de Saúde , Atenção Primária à Saúde , Adolescente , Adulto , Brasil , Estudos de Casos e Controles , Estudos Transversais , Saúde da Família , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Programas Nacionais de Saúde , Satisfação do Paciente , Fatores Socioeconômicos , Inquéritos e Questionários , Adulto JovemRESUMO
RESUMO Objetivo Avaliar atributos essenciais (acesso de primeiro contato, longitudinalidade, integralidade e coordenação da atenção) e derivados (orientação familiar e comunitária) da Atenção Primária em Saúde (APS) na percepção de usuários com e sem deficiência. Método Estudo transversal observacional utilizando o questionário Primary Care Assesment Tool em usuários autodeclarados com e sem deficiência de cinco unidades básicas de saúde de um município no qual 55% da população depende do SUS. Foram atribuídos escores para as respostas dadas a cada atributo de atenção primária à saúde avaliado. Resultados Participaram 93 usuários (67 com e 26 sem deficiência). Não houve diferença estatisticamente significante para nenhum dos atributos na comparação entre pessoas com e sem deficiência. Para o grupo como um todo, obtiveram-se escores acima do critério de corte: Grau de afiliação, Acesso de primeiro contato (utilização), Longitudinalidade e Coordenação da atenção (sistema de informação). Receberam pior avaliação: Acesso de primeiro contato (acessibilidade), Coordenação da atenção (integralidade do cuidado), Integralidade (serviços disponíveis e prestados) e Orientação familiar e comunitária. Os usuários reconhecem que as equipes identificam problemas de locomoção e movimentação, mas que existem falhas no reconhecimento de problemas para ouvir, falar e enxergar; e na orientação das pessoas que necessitam de auxílio e das que prestam cuidados. Conclusão Os usuários com e sem deficiência avaliaram de forma semelhante a atenção que recebem e indicaram fragilidades no reconhecimento de demandas específicas. Há necessidade de mudanças (estruturais e do processo de trabalho) para assegurar acessibilidade, integralidade do cuidado e orientação familiar e comunitária e, assim, melhor qualificar a APS.
ABSTRACT Purpose To evaluate the core (First Contact, Longitudinality, Comprehensiveness, and Coordination of Services) and derivative (Family Orientation, Community Orientation) attributes of primary health care (PHC) from the perspective of users with and without disabilities. Methods Observational, cross-sectional study using the Primary Care Assessment Tool (PCAT) with users with and without disabilities of five basic health units (BHU) in a municipality where 55% of the population depends on the Brazilian Unified Health System (SUS). Scores were assigned to the responses given to each of the PHC attributes evaluated. Results Study participants were 93 (67 physically disabled and 26 without disabilities) PHC users. No statistically significant differences were observed for any attribute on the comparison between the populations. For both groups, the attributes Degree of Affiliation, First Contact - Utilization, Longitudinality, and Coordination of Services - Information System received satisfactory (above cutoff) scores, whereas the attributes First Contact - Accessibility, Coordination of Services - Care Integration, Comprehensiveness, Family Orientation, and Community Orientation received unsatisfactory (below cutoff) scores. Users reported that the health teams are able to satisfactorily identify mobility issues, but there are failures in the recognition of problems of hearing, voice/speech and vision, and in the orientation of services available and services provided. Conclusion Users with and without disabilities evaluated the health care received similarly, indicating fragilities on the recognition of specific demands. Structural and work process changes should be conducted to ensure Accessibility, Comprehensiveness, and Family and Community Orientation, and thus increase the quality of PHC.