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MicroRNAs (miRNAs) are small endogenous non-coding RNA molecules capable of regulating gene expression at the post-transcriptional level either by translational inhibition or mRNA degradation and have recently been importantly related to the diagnosis and prognosis of the most relevant endocrine disorders. The endocrine system comprises various highly vascularized ductless organs regulating metabolism, growth and development, and sexual function. Endocrine disorders constitute the fifth principal cause of death worldwide, and they are considered a significant public health problem due to their long-term effects and negative impact on the patient's quality of life. Over the last few years, miRNAs have been discovered to regulate various biological processes associated with endocrine disorders, which could be advantageous in developing new diagnostic and therapeutic tools. The present review aims to provide an overview of the most recent and significant information regarding the regulatory mechanism of miRNAs during the development of the most relevant endocrine disorders, including diabetes mellitus, thyroid diseases, osteoporosis, pituitary tumors, Cushing's syndrome, adrenal insufficiency and multiple endocrine neoplasia, and their potential implications as disease biomarkers.
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The breeding of alpaca (Vicugna pacos) is one of the most important economic activities in the high Andean areas of Peru. The commercialization of products derived from alpaca represents more than 80% of the income of high-Andean families. However, the infestation of parasites such as Sarcocystis lamacanis in the alpacas causes economic losses that deteriorate the already diminished quality of life of the alpaca breeder. The search for biomarkers that allow the early detection of these parasites is one of the most critical challenges in Peru, a country with the largest population of alpacas worldwide. This work aimed to analyze and quantify the microcysts formed by the parasite and relate them to the troponin cTnI level in the blood serum. Troponins are proteins secreted when there is damage to the cardiac muscle. 60 blood and cardiac tissue samples were collected from Tisco and La Raya slaughterhouses, localities of Caylloma Province in Arequipa, and Chucuito District in Puno, both regions in southern Peru. The cardiac muscle samples were processed with the routine histology technique and stained with hematoxylin and eosin. In addition, serum samples were processed with the ELISA and immunochromatography methods for troponin cTnI. Results were 100% positive for the presence of Sarcocystis lamacanis microcysts in all cardiac muscle samples. The average microcyst quantification per field of 100x were 3.5 and 5.7 for the Tisco and La Raya samples. In addition, several microscopic lesions were observed in the cardiac muscles: microcyst infiltration between muscle fibers, basophilic microcysts with a thick outer membrane and bradyzoites inside, and tissue displacement. On the other hand, all serum blood samples were negative for troponin cTnI, with both methods, ELISA and immunochromatography. For results, we infer troponin cTnI do not can be used as a biomarker for heart damage caused by Sarcocystis lamacanis parasite in alpacas.
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MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) are two main categories of noncoding RNAs (ncRNAs) that can influence essential biological functions in various ways, as well as their expression and function are tightly regulated in physiological homeostasis. Additionally, the dysregulation of these ncRNAs seems to be crucial to the pathogenesis of human diseases. The latest findings indicate that ncRNAs execute vital roles in cancer initiation and progression, and the cancer phenotype can be reversed by modulating their expression. Available scientific discoveries suggest that phytochemicals such as polyphenols, alkaloids, terpenoids, and organosulfur compounds can significantly modulate multiple cancer-associated miRNAs and lncRNAs, thereby inhibiting cancer initiation and development. However, despite promising outcomes of experimental research, only a few clinical trials are currently being conducted to evaluate the therapeutic effectiveness of these compounds. Nevertheless, understanding phytochemical-mediated ncRNA regulation in cancer and the underlying molecular mechanisms on tumor pathophysiology can aid in the development of novel therapeutic strategies to combat this deadly disease.
Assuntos
MicroRNAs , Neoplasias , RNA Longo não Codificante , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Neoplasias/tratamento farmacológico , Neoplasias/genética , Neoplasias/prevenção & controle , Compostos Fitoquímicos/farmacologia , RNA Longo não Codificante/genéticaRESUMO
In Latin America, hematophagous bats are the main reservoirs of rabies virus (RABV) to livestock, to other mammals and, occasionally, to human. Nonetheless, reports of exposure of human and pets to RABV upon aggression by non-hematophagous bats are increasing, possibly facilitated by the synanthropic habits of these bats. We, herein, report the detection and genetic identification of a RABV recovered from an insectivorous bat found sick in a student housing building at the Federal University of Santa Maria, Southern Brazil. Taxonomic characterization identified the captured bat as a member of the genus Nyctinomops, family Molossidae, the group of insectivorous bats. Brain fragments of the bat were positive for RABV antigens by fluorescent antibody test (FAT) and for sequences of the nucleoprotein (N) gene by RT-PCR. The N amplicon was submitted to nucleotide sequencing and analysis, showing that the consensus sequences (SV 33/19) had high identity with RABV sequences of insectivorous bats deposited in GenBank. At phylogenetic tree, the N gene sequences of SV 33/19 clustered with RABV recovered from Nyctinomops laticaudatus, Molossus molossus, and Tadarida lauticaudata bats, and a part of RABV variant 3, 4, and 6, that correspond to Desmodus rotundus, Tadarida brasiliensis, and Lasiurus cinereus, respectively. Although no direct human or domestic animal exposure has been reported, this case strengthens the need for a continuous rabies vaccination in pets in the surrounding areas, since non-hematophagous bats may serve as source of infection for these animals. These findings also call attention for continuous monitoring of populations of synanthropic bats to avoid/prevent human exposure.
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Quirópteros , Vírus da Raiva , Raiva , Animais , Brasil , Quirópteros/virologia , Filogenia , Raiva/veterinária , Vírus da Raiva/genéticaRESUMO
Resumen Objetivos: El melanoma cutáneo presenta un alto potencial metastásico y constituye la fuente extraabdominal más frecuente de lesión del intestino delgado. El diagnóstico de metástasis gastrointestinales es a menudo una expresión de enfermedad avanzada, con una supervivencia media de entre 6 y 9 meses. Materiales y Método: Presentamos el caso de un paciente varón de 63 años diagnosticado de melanoma cutáneo que acudió a urgencias por dolor abdominal y estreñimiento. Se realizó una TC abdominal donde se informó de la existencia de 2 lesiones metastásicas a nivel de intestino delgado que condicionaban oclusión intestinal. Resultados: El paciente fue intervenido quirúrgicamente bajo abordaje laparoscópico con resección de los dos segmentos intestinales afectos y anastomosis intracorpórea. El informe histopatológico confirmó que se trataban de metástasis de melanoma. Discusión: La oclusión intestinal por metástasis de melanoma maligno es muy infrecuente. La cirugía es el tratamiento de elección en pacientes con metástasis intestinales de melanoma. El tratamiento quirúrgico puede mejorar el pronóstico y estaría indicado casos de metástasis únicas o pacientes sintomáticos con intención paliativa. El abordaje mínimamente invasivo ofrece resultados oncológicos similares a la laparotomía.
Aim: Cutaneous melanoma has a high metastatic potential, being the most frequent extra-abdominal source of small bowel metastasis. The diagnosis of gastrointestinal metastases is often an expression of advanced disease, with an average survival of 6-9 months. Materials and Method: We herein present the case of a 63-year-old male patient diagnosed with cutaneous melanoma who arrived to the emergency department complaining of abdominal pain and constipation. An abdominal CT scan was performed, it revealed two metastatic lesions in the small bowel which marked the mechanical obstruction. Results: Patient underwent a laparoscopy and both involved segments were removed. Pathology exam confirmed the diagnosis of melanoma metastases. Discussion: Surgery excision is the treatment of choice in patients with small bowel metastases from melanoma. Surgical management can improve the prognosis and it would be indicated in cases of single metastases or symptomatic patients with a palliative intention. Minimally invasive approach provides similar oncological results as conventional laparotomy. Small bowel obstruction due to metastases of malignant melanoma is extremely rare.
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Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Cutâneas/diagnóstico , Melanoma/diagnóstico , Neoplasias Cutâneas/patologia , Biópsia , Tomografia Computadorizada por Raios X , Laparoscopia , Neoplasias Gastrointestinais/secundário , Obstrução Intestinal/cirurgia , Melanoma/patologiaRESUMO
Resumen Objetivo: Determinar la frecuencia de reingreso hospitalario y sus factores asociados en pacientes sometidos a resecciones hepáticas o pancreáticas en nuestro centro. Metodología: Se revisaron registros de pacientes sometidos a resecciones hepáticas o pancreáticas entre 2012 y 2014. Se registraron variables biodemográficas, quirúrgicas y reingresos hasta 30 días posalta. Se excluyó la cirugía de urgencia, pacientes fallecidos durante la hospitalización, pacientes sometidos a resecciones hepáticas menores a dos segmentos, cirugías no anatómicas o cirugía no resectiva. El análisis estadístico univariable se realizó con prueba χ2 para variables categóricas y T Student/Mann Whitney para variables continuas. El análisis multivariable se realizó con regresión logística. Resultados: Se incluyeron 116 pacientes, 50,9% mujeres. La estadía promedio fue de 14 días. El adenocarcinoma pancreático fue el diagnóstico más frecuente (25,9%) y 40,5% de los procedimientos quirúrgicos fueron pancreatoduodenectomías. La tasa de reingreso global fue 18,1%, mayor en pancreatectomías respecto a hepatectomías (23,7 vs 12,2% respectivamente p < 0,05). Los factores asociados a reingreso fueron: resección de páncreas, leucocitos preoperatorios, complicaciones posoperatorias y tiempo de estadía hospitalaria. Tras el análisis multivariable, sólo el tiempo hospitalario se asocia de forma independiente al reingreso precoz [OR 1,2 IC 95% 1,1-1,5 (p = 0,001)]. Conclusión: La estadía hospitalaria prolongada es un factor de riesgo consistente en la literatura para la rehospitalización posterior a resecciones hepáticas o pancreáticas. La tasa de reingreso posterior a resecciones hepáticas o pancreáticas es elevada, incluso en centros de alto volumen. Recomendamos el uso de este parámetro como un nuevo instrumento de medición de calidad en los resultados quirúrgicos en nuestro país.
Aim: To determine readmission rates and its associated factors in patients undergoing pancreatic and hepatic resections at our center. Matherial and Methods: Perioperative variables of patients undergoing pancreatic and hepatic resections between 2012-2014 were reviewed. Demographic and perioepartive data, as well as up —to postoperative day 30— readmisson rates were analyzed. Emergency cases, postoperative mortality and/or patients undergoing less extensive surgery (less than 2 Couinaud's segments, non-anatomical resections and non resective cases such as bilioenteric anastomoses) were excluded. Readmission associated factors were identified using both univariate (χ2 for categorical and t-student's/Mann-Whitney for continuous variables) and multivariate (logistic regression) analysis. Results: 116 cases were included, 50.9 % female. Mean postoperative stay was 14 days. Pancreatic adenocarcinoma was the most frequent diagnosis (25.9%), and the 40.5% of surgical procedures were pancreaticoduodenectomy. Overall 30-day readmission rate was 18.1%, with a 23.7% for pancreatic resections and 12.2% for hepatic resections. According to univariate analysis; readmission associated factors were: pancreatic resection, preoperative White cell count, the development of postoperative complications and postoperative length of stay. On Multivariate analysis only postoperative stay was the only significant associate factor [OR 1,2 CI 95% 1.1- 1.5 (p = 0.001)]. Conclussion: Readmission rates after pancreatic and hepatic resections are elevated, even in high-volume centers. Postoperative length of stay is a consistent risk factor for readmission after these type of surgeries. We highly recommend including this parameter as a quality marker of our surgical results in our country.
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Humanos , Masculino , Feminino , Pancreatectomia/estatística & dados numéricos , Readmissão do Paciente/estatística & dados numéricos , Hepatectomia/estatística & dados numéricos , Pancreatectomia/efeitos adversos , Análise Multivariada , Fatores de Risco , Hepatectomia/efeitos adversosRESUMO
Candida kefyr has been considered both a food-spoiling agent and a type of yeast with fermentation properties. In this study, the authors have evaluated the antimicrobial activity of a coconut oil-in-water emulsion associated to the presence of C. kefyr. Fresh coconut kernels were used to obtain the coconut oil-in-water emulsion, the sterile coconut oil-in-water emulsion by decantation, and the coconut oil by means of a heating process. Commercial virgin coconut oil was also used. Agar diffusion, minimal inhibitory concentration and minimal bactericidal concentration (MIC/MBC) techniques were employed to evaluate antimicrobial activity against E. coli and S. epidermidis. The C. kefyr isolate was identified and confirmed. Coconut milk-derived fatty acids were characterized by acid index and thin layer chromatography. Scanning electronic microscopy was performed to evaluate the morphology of the microorganisms. Lipase activity of C. kefyr isolate was also detected. Coconut oil-in-water emulsion associated to C. kefyr was active against both bacteria. Thin layer chromatography confirmed the presence of triglycerides and free fatty acids. The acid index showed higher acidity potential for the coconut oil-in-water emulsion. The microscopic images showed antibacterial action through the formation of membrane holes' and demonstrated yeast shape. All the above show new potentials for C. kefyr and coconut oil-in-water emulsion in food technology.
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Humanos , Feminino , Idoso , Pneumatose Cistoide Intestinal/cirurgia , Pneumatose Cistoide Intestinal/etiologia , Traumatismos Abdominais/complicações , Vesícula Biliar/cirurgia , Vesícula Biliar/lesões , Colecistectomia , Pneumatose Cistoide Intestinal , Ruptura , Tomografia Computadorizada por Raios X , Veia Porta/lesõesRESUMO
Molecular techniques have revealed a high prevalence of Pneumocystis colonization in wild mammals. Accurate quantification of Pneumocystis sp. is essential for the correct interpretation of many research experiments investigating this organism. The objectives of this study were to detect the presence of Pneumocystis sp. in bats by qPCR, and to distinguish colonization from infection. Probes and primers for real time PCR (qPCR) were designed based on the gene of major surface glycoprotein (MSG) of Pneumocystis sp., in order to analyze 195 lung tissue samples from bats captured (2007-2009). All samples were also analyzed by nested PCR, using oligonucleotide primers designed for the gene encoding the mitochondrial small subunit rRNA (mtSSU rRNA) to confirm the results. The qPCR assay was standardized using a standard curve made with the DNA extracted from bronchoalveolar lavage positive for Pneumocystis jirovecii. The average Ct was found to be between 13 and 14 (calibration curve) for the detection of infection with Pneumocystis sp. and above these values for colonization. It was considered as negative samples the ones that had Ct values equal to 50. Out of the total 195 samples, 47 (24.1%) bat lung DNA samples were positive for Pneumocystis sp. by qPCR. The most common bat species found were: Tadarida brasiliensis (23.4%), Histiotus velatus (17.0%), Desmodus rotundus (14.9%) and Molossus molossus (8.5%). The average cycle threshold of the positive samples (bats) was 25.8 and standard deviation was 1.7. The DNA samples with Ct values greater than 14 suggest that these animals might be colonized by Pneumocystis sp. Results obtained in this study demonstrated the usefulness of the qPCR procedure for identification of Pneumocystis sp. and for distinction between its colonizing or infectious status in bats.
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Portador Sadio/veterinária , Quirópteros/microbiologia , Reservatórios de Doenças/microbiologia , Infecções por Pneumocystis/transmissão , Pneumocystis/isolamento & purificação , Animais , Brasil , Líquido da Lavagem Broncoalveolar/microbiologia , Portador Sadio/epidemiologia , Portador Sadio/microbiologia , Quirópteros/classificação , DNA Fúngico/análise , DNA Fúngico/genética , Proteínas Fúngicas/genética , Especificidade de Hospedeiro , Pulmão/microbiologia , Glicoproteínas de Membrana/genética , Pneumocystis/genética , Pneumocystis carinii/genética , Pneumocystis carinii/isolamento & purificação , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase em Tempo Real , Ribotipagem , Especificidade da EspécieAssuntos
Humanos , Feminino , Pessoa de Meia-Idade , Falso Aneurisma/cirurgia , Falso Aneurisma/etiologia , Fístula Arteriovenosa/cirurgia , Fístula Arteriovenosa/etiologia , Procedimentos Neurocirúrgicos/efeitos adversos , Angiografia , Falso Aneurisma , Disco Intervertebral/cirurgia , Dor Abdominal/etiologia , Dor Pós-Operatória/etiologia , Fístula Arteriovenosa , Tomografia Computadorizada por Raios XRESUMO
The biodegradation of effluent from an industrial paper mill was monitored in relation to the parameters total phenols, low molar mass phenols and color during its incubation with laccase (Novozymes), laccase-HBT and peroxidase (Merck). The results revealed that the laccase reduced the effluent color by 37% and the peroxidase by 43%. Also, the enzymes had a great efficiency in the reduction of color, total phenols and high molar mass compounds contained in the effluent, indicating that depolymerization occurred.
A biodegradação dos efluentes de uma indústria de papel e celulose foi monitorada em relação aos parâmetros de fenóis totais, fenóis de baixa massa molar e cor durante a sua incubação com lacase (Novozymes), lacase-HBT e peroxidase (Merck). Os resultados revelaram que a lacase reduziu a cor do efluente em 37% e a peroxidase em 43%. Também foi possível observar que as enzimas possuiram grande eficiência na redução de cor, compostos fenólicos totais e compostos fenólicos de alta massa molar contidos no efluente, indicando, desta forma, que a despolimerização ocorreu.
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Biotransformação , Indústria de Papel e Celulose , Efluentes Industriais , Peroxidase/administração & dosagem , Lacase/administração & dosagemRESUMO
ABSTRACT The biodegradation of effluent from an industrial paper mill was monitored in relation to the parameters total phenols, low molar mass phenols and color during its incubation with laccase (Novozymes), laccase-HBT and peroxidase (Merck). The results revealed that the laccase reduced the effluent color by 37% and the peroxidase by 43%. Also, the enzymes had a great efficiency in the reduction of color, total phenols and high molar mass compounds contained in the effluent, indicating that depolymerization occurred.
RESUMO A biodegradação dos efluentes de uma indústria de papel e celulose foi monitorada em relação aos parâmetros de fenóis totais, fenóis de baixa massa molar e cor durante a sua incubação com lacase (Novozymes), lacase-HBT e peroxidase (Merck). Os resultados revelaram que a lacase reduziu a cor do efluente em 37% e a peroxidase em 43%. Também foi possível observar que as enzimas possuiram grande eficiência na redução de cor, compostos fenólicos totais e compostos fenólicos de alta massa molar contidos no efluente, indicando, desta forma, que a despolimerização ocorreu.
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A sandwich ELISA (S-ELISA) was developed to detect antibodies to rabies virus in seraof different species. The test was performed as follows: ELISA plates were coated withpolyclonal mouse/anti-rabies antibodies for 2 hours at 37ºC. After adsorption, plateswere washed and non-specific binding blocked with 2% powdered milk. In a separateplate, serial threefold dilutions of test sera were incubated with inactivated rabies virusantigen. The mixtures were then placed on the rabies antibody-coated plates andincubated. These were then washed and incubated with polyclonal rabbit/anti-rabiesantibodies. Subsequently, a rabbit/IgG-peroxidase conjugate was added and platesincubated. After washing, the chromogen (ABTS with 0.15% H2O2) was added to platesand after incubation for 30 min were read in a spectrophotometer (OD405). To validatethe S-ELISA, 128 serum samples including humans, cattle, hematophagous and nonhaematophagous bats, mice, marmosets, ocelots - Leopardus pardalis, raccoons -Procyon lotor, jaguarondi - Herpailurus yaguarondi, fox - Cerdocyon thous and coati -Nasua nasua, were tested and compared to a standard fluorescent antibody virusneutralization test (FAVN). In comparison to FAVN, the S-ELISA showed highsensitivity (82.98%) and specificity (100%), with an accuracy of 87.5%. Subsequently,738 serum samples from different species were tested in the S-ELISA. Antibodies torabies were detected by S-ELISA in all species tested, with the exception of the threeserum samples from raccoons. The S-ELISA was shown to be a serological test of lowcost that can be easily implemented in diagnostic laboratories. In addition, no liveanimals, infectious virus, cell culture or fluorescence microscopy are required forperformance of the test. This is an additional advantage of the S-ELISA over othermethods of rabies antibody detection.
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Anticorpos Antivirais , Estudos Soroepidemiológicos , Raiva , Vírus da RaivaRESUMO
The present study corresponds to a quali-quantitative research, which inquired into user's perception of the Recovery Program of the Child in Nutritional Risk Nutrir Más, in Santa Fe city urban area in the last trimester of 2007. Specifically, it aimed at identifying the perceptions related to the program's components: training workshops, health controls and alimentary assistance through tickets; program's positive and negative aspects; usefulness of knowledge gained in the upbringing daily tasks; and the relationship between beneficiaries and program's leaders in the workplace and the health staff. About 50 percent of the beneficiaries who had been in the program for more than a year, participated in a survey through a semi-structured interview. In general, the users reported a high level of satisfaction towards the activities proposed by the program and highlighted its importance for educational actions, the improvement of their knowledge and alimentary practices towards their underweight children, the diversification of daily feeding and the reinforcement of the emotional bond between mother and child. This research recognizes the importance of perception studies because they allow to improve the program's management and to be aware of the user's satisfaction, as well.
El presente estudio corresponde a una investigación cuali-cuantitativa que indagó la percepción de los usuarios del Programa de Recuperación del Niño en Riesgo Nutricional "NUTRIR MAS" del área urbana de la ciudad de Santa Fe en el último trimestre del 2007. Se buscó identificar las percepciones vinculadas a los componentes del programa: actividades educativas, controles de salud y prestación alimentaria a través de tickets; con el propósito de identificar aspectos positivos y negativos del mismo, la utilidad de los conocimientos impartidos y la relación entre los usuarios y los referentes del programa en terreno y el equipo de salud. Se realizó una encuesta semiestructurada al 50 por ciento de los usuarios de la ciudad de Santa Fe que tenían una permanencia en el programa mayor a un año. El 99 por ciento de los encuestados reportó un alto nivel de satisfacción hacia las actividades propuestas por el programa y destacaron su valor por las acciones educativas, el mejoramiento de sus conocimientos y prácticas alimentarias, la diversificación de la alimentación cotidiana y el fortalecimiento del vínculo madre-hijo. Esta investigación reconoce la importancia de los estudios de percepción porque permite mejorar la gestión del programa adaptando las estrategias de intervención a la población destinataria.
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Humanos , Masculino , Feminino , Criança , Nutrição da Criança , Programas de Nutrição , Nutrição dos Grupos Vulneráveis , Percepção , Pais/psicologia , Argentina , Estudos Transversais , Educação Alimentar e Nutricional , Preferências Alimentares , Inquéritos Nutricionais , Risco , Grupos de Risco , Comportamento do Consumidor , Área UrbanaRESUMO
When Avibacterium paragallinarum reference strain 0083 (serovar A) was grown in an iron-restricted culture medium, the expression of the 60, 68 and 93 kDa outer membrane proteins increased as compared with normal media. Sera of chickens experimentally infected with Av. paragallinarum recognized these iron-restriction induced proteins, suggesting their expression in vivo. The three outer membrane proteins were identified as transferrin receptor and iron transport proteins by mass spectroscopy and a search in sequence databases. As these proteins have been reported to be regulated by the Fur protein in many bacteria, we investigated, through molecular methods, the presence of the fur gene in Av. paragallinarum. A candidate fur gene of Av. paragallinarum was amplified by polymerase chain reaction using complementary primers to conserved regions of fur gene sequences from members of the Pasteurellaceae family. The nucleotide sequence of the cloned gene, from ATG to TAA stop codon, was 453 base pairs in length and the deduced amino acid sequence showed 94% identity with Fur sequences of Actinobacillus pleuropneumoniae and Haemophilus ducreyi. The Av. paragallinarum deduced Fur protein (17.8 kDa) amino acid sequence contains the N-terminal helix-turn-helix DNA-binding domain and the two iron-binding sites in the C-terminal end, typical of other described Fur proteins. The study of iron-restriction-induced proteins and the mechanism regulating their expression could lead to an understanding of the responses of Av. paragallinarum to survive in an iron-restricted environment on host mucosal surfaces.
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Proteínas de Bactérias/genética , Pasteurellaceae/genética , Receptores da Transferrina/metabolismo , Proteínas Repressoras/genética , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sequência de Bases , Western Blotting , Clonagem Molecular , Biologia Computacional , Primers do DNA/genética , Eletroforese em Gel de Poliacrilamida , Ferro/metabolismo , Espectrometria de Massas , Dados de Sequência Molecular , Receptores da Transferrina/genética , Proteínas Repressoras/metabolismo , Análise de Sequência de DNA , Homologia de SequênciaRESUMO
A Brucelose é uma antropozoonose de importância tanto para saúde pública quanto para a economia das regiões onde ocorre, devido à alta taxa de aborto e infertilidade em rebanhos infectados. O animal infectado é a mais importante fonte de transmissão ao homem, que pode ocorrer de forma ocupacional ou pela ingestão de produtos contaminados. A brucelose caprina é a que desenvolve maior virulência para o homem. No atual projeto foi possível investigou-se a soroprevalência para brucelose em diferentes rebanhos da Bahia, onde, assim como em todo o nordeste do Brasil, a caprinocultura representa uma das principais fontes de renda e de alimento para a população. Amostras de soro de 400 caprinos provenientes de rebanhos das regiões de Amélia Rodrigues, Feira de Santana e Jaguarari, na Bahia, foram testadas pelo método de soro aglutinação direta com antígeno brucélico rosa-bengala em prova rápida para triagem, Todas as amostras positivas por este método foram submetidas a prova lenta em tubo, confirmando-se 03 casos positivos numa região que possui poucos relatos bibliográficos de endemia para brucelose.
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A Brucelose é uma antropozoonose de importância tanto para saúde pública quanto para a economia das regiões onde ocorre, devido à alta taxa de aborto e infertilidade em rebanhos infectados. O animal infectado é a mais importante fonte de transmissão ao homem, que pode ocorrer de forma ocupacional ou pela ingestão de produtos contaminados. A brucelose caprina é a que desenvolve maior virulência para o homem. No atual projeto foi possível investigou-se a soroprevalência para brucelose em diferentes rebanhos da Bahia, onde, assim como em todo o nordeste do Brasil, a caprinocultura representa uma das principais fontes de renda e de alimento para a população. Amostras de soro de 400 caprinos provenientes de rebanhos das regiões de Amélia Rodrigues, Feira de Santana e Jaguarari, na Bahia, foram testadas pelo método de soro aglutinação direta com antígeno brucélico rosa-bengala em prova rápida para triagem, Todas as amostras positivas por este método foram submetidas a prova lenta em tubo, confirmando-se 03 casos positivos numa região que possui poucos relatos bibliográficos de endemia para brucelose.
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The objective of the present study is to investigate the perspective of mothers regarding the care given by academics of nursing to their newborn in a neonatal intensive therapy unit. This is a qualitative research based on a phenomenological approach which has as its philosophical framework the thought of Martin Heidegger expressed in his book Being and Time. The data used in the investigation were interviews given by ten mothers who had their newborns in a neonatal intensive therapy unit of a university hospital in the city of Rio de Janeiro. The interpretation of the data collected revealed that mothers viewed the nursing academics as solicitous beings regarding the care given to their newborns. They also acknowledged that these students were engaged in the assistance given and concerned with what was being done and to whom it was being done.
Assuntos
Terapia Intensiva Neonatal/normas , Mães , Cuidados de Enfermagem/normas , Filosofia em Enfermagem , Estudantes de Enfermagem , Feminino , Humanos , Recém-Nascido , Unidades de Terapia Intensiva NeonatalRESUMO
The present study was performed to investigate the effect of chloroform-methanol (1:1) extracts derived from Baccharis heterophylla H. B. K (Asteraceae), Desmodium grahami Gray (Leguminosae), Dyssodia pinnata var. pinnata Rob. (Asteraceae), Gentiana spathacea H. B. K. (Gentianaceae), Loeselia mexicana Brand. (Polemoniaceae), Selaginella pallescens Spring. (Selaginellaceae) and Zornia diphylla Pers. (Fabaceae) on the spontaneous contractions of isolated rat ileum. The concentration-response curves and IC50 values were obtained and the respective relaxant potencies were compared with that of the CHCl3-MeOH (1:1) extract of Datura lanosa. The data showed that all the extracts produce a concentration-dependent inhibition of spontaneous ileum contractions. In addition, the antimicrobial activity of the extracts against pathogenic enterobacteria was tested. The pharmacological actions shown by the extracts of the selected species tend to support ethnomedical use of the plants for the treatment of gastrointestinal disorders.