1.
Biotechnol Lett
; 26(16): 1269-72, 2004 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-15483385
RESUMO
Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 degrees C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l(-1) in the culture supernatant after 160 h of growth.