RESUMO
Acanthamoeba griffini is known to cause amoebic keratitis (AK); its main causes are inadequate hygiene when contact lenses are handled and/or its prolonged use at night, as well as the use of contact lenses during underwater activities. The most used treatment for AK is the combination of propamidine isethionate combined with polyhexamethylene biguanide, which disrupts the cytoplasmic membrane, and damages cellular components and respiratory enzymes. We proposed an immunoconjugate treatment obtained from Acanthamoeba immunized rabbit serum combined with propamidine isethionate; the corneas of hamsters inoculated with A. griffini (MYP2004) were treated with the combined, at 1, 2, and 3 weeks. Propamidine isethionate is frequently used for AK treatment, in vivo study we are found IL-1ß and IL-10 expression and caspase 3 activity is significantly increased with respect to the group that was inoculated with the amoeba without receiving any treatment, suggesting that it may be an effect of the toxicity of this drug on the corneal tissue. Application of the immunoconjugate showed enhanced amoebicidal and anti-inflammatory activities, with comparison to propamidine isethionate only. The aim of this study is to evaluate the effect of the immunoconjugate of propamidine isethionate and polyclonal antibodies as a treatment of AK in golden hamsters (Mesocricetus auratus).
RESUMO
The aim of this study was to evaluate the inflammation process that resulted from the inoculation of Wistar Rats with Acanthamoeba griffini, a virulent T3 Acanthamoeba genotype that produces keratitis. Haematoxylin and eosin, periodic acid stain, immunohistochemistry and morphometry were used to analyse tissues from rats of an Acanthamoeba keratitis (AK) model. Two weeks after inoculating the rats with A griffini trophozoites, the thickness of the stroma had diminished, followed by an increase in thickness at 4 weeks. At the latter time, an abundance of inflammatory infiltrate cells was observed, some found to express IL-1ß, IL-10 and/or caspase 3. Intercellular adhesion molecule-1 was expressed in corneal blood vessels amid the abundant vascularization characteristic of the development of AK. Through an immunohistochemical technique, trophozoites were detected at 2 and 4 weeks post-inoculation. By 8 weeks, there were a low number of trophozoites and cysts and the corneas of infected rats were similar in thickness to those of the controls. Thus, the rats were capable of healing experimental AK in the present rat model. Diverse immunological mechanisms regulated the inflammatory process in acute AK induced by A griffini in a murine model.
Assuntos
Ceratite por Acanthamoeba/patologia , Acanthamoeba/fisiologia , Acanthamoeba/classificação , Ceratite por Acanthamoeba/imunologia , Animais , Apoptose , Caspase 3/análise , Córnea/patologia , Modelos Animais de Doenças , Feminino , Humanos , Molécula 1 de Adesão Intercelular/análise , Interleucina-10/análise , Interleucina-1beta/análise , Camundongos , Ratos , Ratos Wistar , Trofozoítos/fisiologiaRESUMO
En este trabajo se hace una arevision de los casos humanos parasitados por Bertiella mucronata y Bertiella studeri (Cestoda: Anoplocephalidae), que incluye la casuistica internacional y bibliografia actualizada. Se analizan varios aspectos de esta zoonosis como son: taxonomia, ciclo biologico, epidemiologia, patologia, diagnostico, control, prevencion y terapeutica. Se discuten aspectos relacionados con la potencialidad zoonotica creciente de esta parasitosis