RESUMO
64 Haemophilus influenzae strains circulating in Havana City during a year were characterized by the carbohydrate fermentation method for the first time in Cuba. The fermentative pattern D was the most frequently found. Patterns D and G together were 72% of the total of strains studied. The combination of the carbohydrate fermentation with serotyping and biotyping allowed a greater differentiation of strains (14 groups). Patterns A, B, C and F appeared in children over 6 months of age, and pattern G in the group from 6 to 18. Patterns D and G predominated in the bacterial meningoencephalitis. A higher heterogeneity was observed among the strains isolated from acute respiratory infections. Some of the advantages of the Haemophilus influenzae strains subtyping method are stressed, such as: simplicity, easiness to be applied and interpreted, and the fact that it is not necessary a qualified personnel or a specialized laboratory for its implementation.
Assuntos
Técnicas de Tipagem Bacteriana , Metabolismo dos Carboidratos , Haemophilus influenzae/classificação , Proteínas de Bactérias/análise , Pré-Escolar , Fermentação , Infecções por Haemophilus/microbiologia , Haemophilus influenzae/isolamento & purificação , Haemophilus influenzae/metabolismo , Humanos , Lactente , Recém-Nascido , Testes de Fixação do Látex , Estudos Prospectivos , Especificidade da EspécieRESUMO
A study is made of non-fermenting gram-negative bacterial strains isolated at the Microbiology Lab, "Centro Habana" Pediatric Teaching Hospital. The strains studied come from various types of samples with prevalence of exudates and smears. A definite biotyping of 100% of strains is made. 90.0% are classified within the genus Pseudomonas. In addition, organisms of the genera Acinetobacter, Alcaligenes, Achromobacter, and Moraxella are identified. The most frequent species turned out to be Pseudomonas aeruginosa, which accounted for 68.4% of the total.
Assuntos
Bactérias Aeróbias Gram-Negativas/classificação , Técnicas de Tipagem Bacteriana , Cuba , Fermentação , Bactérias Aeróbias Gram-Negativas/metabolismo , Hospitais Pediátricos , HumanosRESUMO
The use of King A and King B media for the faster diagnosis of Pseudomonas aeruginosa is introduced in the Microbiology Laboratory of Centro Habana Pediatric Teaching Hospital. The appropriate use of these media allows to classify as Pseudomonas aeruginosa 77.2% of the Pseudomonas sp. strains studied, which represent 95.2% of the total of strains identified as Pseudomonas aeruginosa, according to Hugh and Gilardi conventional technique. There are no significant differences in the implementation of the two procedures (X2: 0.5; p less than 0.05). The demonstrations of pyocines with King A medium is significantly superior to the method for extraction and solubility of the pigment in chloroform (X2: 15.05; p less than 0.01).