Assuntos
Infecções por Escherichia coli/microbiologia , Escherichia coli O157/isolamento & purificação , Pré-Escolar , Cuba , Infecções por Escherichia coli/epidemiologia , Escherichia coli O157/classificação , Escherichia coli O157/genética , Escherichia coli O157/patogenicidade , Humanos , Lactente , Fenótipo , Reação em Cadeia da Polimerase , Estudos RetrospectivosRESUMO
The introduction of a mollecular method to identify the Entoviruses based on the amplification sequecing and phylogenetic analysis of protein VPI was described. It was proved that this method reduces significantly the time required for the identification of the isolated Entoviruses and that it is very useful in the characterization of isolates which are difficult to typify by the routine immunoloigical reagents. As it is a very fast technqiue, its use is very important during epidemics to determine the causal agent rapidly.
Assuntos
Enterovirus/genética , Enterovirus/isolamento & purificação , Proteínas Estruturais Virais/genética , Humanos , Técnicas de Amplificação de Ácido NucleicoRESUMO
For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.