RESUMO
Se realizó un estudio descriptivo prospectivo con el propósito de determinar los efectos cardiorrespiratorios en el niño durante su primera visita odontológica en la Clínica Estomatológica Pediátrica de la Universidad Alas Peruanas, sin la presencia de alguna condición pre-existente, como dolor, las visitas dentales traumáticas previas, uso de medicamentos y enfermedades sistémicas. Material y métodos: Se registró la frecuencia cardiaca, saturación de oxígeno, presión arterial, temperatura corporal y frecuencia respiratoria de 70 niños antes de la primera visita odontológica (sala de espera) y después de la atención (sala clínica) con un oximetro de pulso dactilar, tensiómetro digital, termómetro frontal y un reloj de pulsera. Resultados: mostro una temperatura corporal basal y post operatoria de 36°C y 36,l°C; respectivamente (p= 0,057). La presión arterial sistólica basal fue de 96 mm Hg y post operatoria de 93 mm Hg (p= 0,156), mientras que la diastólica basal fue de 63 mm Hg y post operatoria de 63 mm Hg (p= 0,624). La saturación de oxigeno basal de 96% y post operatoria 93% (p= 0,001). La frecuencia cardiaca basal fue de 88 ppm y post operatoria de 85 ppm (p= 0,229). La frecuencia respiratoria basal fue de 20 rpm y post operatoria de 20 rpm (p= 0,006). Se concluyó que existen cambios cardiorrespiratorios significativos en la primera visita odontológica, al presentar diferencias significativas en los valores de la saturación de oxígeno y frecuencia respiratoria, registradas antes y después de la atención odontológica...
A descriptive, cross-sectional study was performed to determine the cardiorespiratory effects in child's first dental visit to the Pediatric Dental Clinic at Alas Peruanas University, without the presence of any pre-existing condition, such as pain, previous traumatic dental visits, use of medications and systemic diseases. Material and methodos: To accomplish this, heart rate, oxygen saturation, blood pressure, body temperature and respiratory rate were recorded on 70 children before (waiting room) and after first dental visit (operating room) using a finger pulse oximeter, digital sphygmomanometer, thermometer and a wristwatch. Results: showed basal and postoperative body temperature registered 36° C and 36.1° C, respectively. Basal systolic blood pressure registered 96 mm Hg and postoperative 93 mm Hg, and basal diastolic blood pressure registered 63 mm Hg and postoperative 63 mm Hg. Basal oxygen saturation registered 96% and postoperative 93%. Basal heart rate registered 88 bpm and postoperative 85 bpm. Basal respiratory rate registered 20 bpm and postoperative 20 bpm. The author concluded that significant cardiorespiratory changes exist during first dental visit, existing significant differences in oxygen saturation and respiratory rate recorded before and after dental care...
Assuntos
Humanos , Masculino , Feminino , Criança , Ansiedade ao Tratamento Odontológico , Assistência Odontológica para Crianças , Visita a Consultório Médico , Estudos de Avaliação como Assunto , Estudos de Avaliação como Assunto , Epidemiologia DescritivaRESUMO
Rabbits were immunized using intact larvae or homogenates from Ancylostoma duodenale. Antisera were tested by immunodiffusion. The homogenates promote the formation of antibodies but the intact worms were not able to induce them. The antisera were partially purified by precipitation with amonium sulphate 40% saturation and filtration through Sephadex G-200. The purified material was attached to Sepharose 6B and used as immunoabsorbent for the isolation of the antigens from the soluble extracts of parasites. The isolated antigens were used in order to obtain new antisera. These antisera were used for the preparation of more efficient immunoabsorbent which allow to isolate new antigens that gave three precipitation lines by immunodiffusion. The polyacrylamide gel electrophoresis of crude homogenate discriminate 12 components, and the electrophoresis of the isolated antigens gave only 3 bands.
Assuntos
Ancylostoma/imunologia , Formação de Anticorpos , Antígenos/isolamento & purificação , Adjuvantes Imunológicos/administração & dosagem , Animais , Cromatografia de Afinidade , Cromatografia em Gel , Eletroforese em Gel de Poliacrilamida , Soros Imunes/isolamento & purificação , Imunodifusão , CoelhosRESUMO
Rabbits were immunized using intact larvae or homogenates from Ancylostoma duodenale. Antisera were tested by immunodiffusion. The homogenates promote the formation of antibodies but the intact worms were not able to induce them. The antisera were partially purified by precipitation with amonium sulphate 40
saturation and filtration through Sephadex G-200. The purified material was attached to Sepharose 6B and used as immunoabsorbent for the isolation of the antigens from the soluble extracts of parasites. The isolated antigens were used in order to obtain new antisera. These antisera were used for the preparation of more efficient immunoabsorbent which allow to isolate new antigens that gave three precipitation lines by immunodiffusion. The polyacrylamide gel electrophoresis of crude homogenate discriminate 12 components, and the electrophoresis of the isolated antigens gave only 3 bands.
RESUMO
Rabbits were immunized using intact larvae or homogenates from Ancylostoma duodenale. Antisera were tested by immunodiffusion. The homogenates promote the formation of antibodies but the intact worms were not able to induce them. The antisera were partially purified by precipitation with amonium sulphate 40
saturation and filtration through Sephadex G-200. The purified material was attached to Sepharose 6B and used as immunoabsorbent for the isolation of the antigens from the soluble extracts of parasites. The isolated antigens were used in order to obtain new antisera. These antisera were used for the preparation of more efficient immunoabsorbent which allow to isolate new antigens that gave three precipitation lines by immunodiffusion. The polyacrylamide gel electrophoresis of crude homogenate discriminate 12 components, and the electrophoresis of the isolated antigens gave only 3 bands.