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BACKGROUND: To describe an oncolytic adenovirus (OAd) encoding SP-SA-E7-4-1BBL that is capable of inducing tumor regression in therapeutic assays. Herein, we tested whether the antitumor effect is given by the induction of a tumor-specific immune response, as well as the minimum dose needed to elicit antitumor protection and monitor the OAd biodistribution over time. METHODS AND RESULTS: C57BL/6 mice (n = 5) per group were immunized twice with OAds encoding SP-SA-E7-4-1BBL, SA-E7-4-1BBL, or SP-SA-4-1BBL and challenged with TC-1 cancer cells. The DNA construct SP-SA-E7-4-1BBL was employed as a control via biolistic or PBS injection. Groups without tumor development at 47 days were rechallenged with TC-1 cells, and follow-up lasted until day 90. The minimum dose of OAd to induce the antitumor effect was established by immunization using serial dilution doses. The cytometry bead assay and the ELISpot assay were used to evaluate cytokine release in response to ex vivo antigenic stimulation. The distribution profile of the OAd vaccine was evaluated in the different organs by histological, immunohistochemical and qPCR analyses. The OAd SP-SA-E7-4-1BBL-immunized mice did not develop tumors even in a rechallenge. A protective antitumor effect was observed from a dose that is one hundredth of most reports of adenoviral vaccines. Immunization with OAd increases Interferon-gamma-producing cells in response to antigen stimulation. OAd was detected in tumors over time, with significant morphological changes, contrary to nontumor tissues. CONCLUSIONS: The OAd SP-SA-E7-4-1BBL vaccine confers a prophylactic, safe, long-lasting, and antigen-dependent antitumor effect mediated by a Th1 antitumor immune response.
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Vacinas Anticâncer , Neoplasias , Animais , Camundongos , Papillomavirus Humano 16 , Ligante 4-1BB/genética , Ligante 4-1BB/farmacologia , Distribuição Tecidual , Camundongos Endogâmicos C57BL , Adenoviridae/genética , Imunidade , Neoplasias/terapiaRESUMO
BACKGROUND: Occupational accidents in the plumbing activity in the construction sector in developing countries have high rates of work absenteeism. The productivity of enterprises is heavily influenced by it. OBJECTIVE: To propose a model based on the Plan, Do, Check, and Act cycle and data mining for the prevention of occupational accidents in the plumbing activity in the construction sector. METHODS: This cross-sectional study was administered on a total of 200 male technical workers in plumbing. It considers biological, biomechanical, chemical, and, physical risk factors. Three data mining algorithms were compared: Logistic Regression, Naive Bayes, and Decision Trees, classifying the occurrences occupational accident. The model was validated considering 20% of the data collected, maintaining the same proportion between accidents and non-accidents. The model was applied to data collected from the last 17 years of occupational accidents in the plumbing activity in a Colombian construction company. RESULTS: The results showed that, in 90.5% of the cases, the decision tree classifier (J48) correctly identified the possible cases of occupational accidents with the biological, chemical, and, biomechanical, risk factors training variables applied in the model. CONCLUSION: The results of this study are promising in that the model is efficient in predicting the occurrence of an occupational accident in the plumbing activity in the construction sector. For the accidents identified and the associated causes, a plan of measures to mitigate the risk of occupational accidents is proposed.
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Acidentes de Trabalho , Indústria da Construção , Mineração de Dados , Humanos , Mineração de Dados/métodos , Estudos Transversais , Acidentes de Trabalho/prevenção & controle , Acidentes de Trabalho/estatística & dados numéricos , Masculino , Adulto , Colômbia/epidemiologia , Fatores de Risco , Teorema de Bayes , Árvores de Decisões , Modelos Logísticos , AlgoritmosRESUMO
Scedosporium apiospermum is an opportunistic pathogen that can cause pulmonary infections in both immunosuppressive and immunocompetent patients. Cytokines are molecules that mediate the immune response to promote or eliminate fungal infections. In this work, we evaluated the cytokines profile in the lung and serum of mice infected with Scedosporium apiospermum. We found early production of IL-6, IL-1ß and TNF-α cytokines in the lung of infected mice during the first 5 days of infection. We suggest that release of pro-inflammatory cytokines could play a role in the control of fungal invasion.
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Micoses , Pneumonia , Scedosporium , Animais , Antifúngicos/uso terapêutico , Citocinas , Pulmão , Camundongos , Micoses/tratamento farmacológico , Pneumonia/tratamento farmacológicoRESUMO
A substantial corpus of experimental research indicates that in many species, long-term habituation appears to depend on context-stimulus associations. Some authors have recently emphasized that this type of outcome supports Wagner's priming theory, which affirms that responding is diminished when the eliciting stimulus is predicted by the context where the animal encountered that stimulus in the past. Although we agree with both the empirical reality of the phenomenon as well as the principled adequacy of the theory, we think that the available evidence is more provocative than conclusive and that there are a few nontrivial empirical and theoretical issues that need to be worked out by researchers in the future. In this paper, we comment on these issues within the framework of a quantitative version of priming theory, the SOP model.
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This paper presents an open-source online tool for introducing psychology students to the major theoretical and empirical facts of habituation. The tool was designed in a way that combines theory and data through simulated experiments. The simulations exemplify how the priming theory of Allan R. Wagner accounts for the set of behavioral characteristics of habituation proposed by Richard F. Thompson and W. Alden Spencer in 1966. Through this interactive platform, the user can learn the basics of the theory and examine how it accounts for the empirical facts with different parameters. Instructions and commands are provided in three languages: English, Spanish, and Portuguese.
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Habituação Psicofisiológica , Laboratórios , HumanosRESUMO
RESUMEN El presente trabajo busca establecer la forma en que la inversión social responsable tiene el potencial de dinamizar el desarrollo empresarial de un país y, al mismo tiempo, asumir los objetivos de sostenibilidad, como propósitos propios de las actividades productivas en los agronegocios. Para ello, se propone una aproximación a un modelo teórico ideal, que expone la dinámica de la inversión social responsable, como impulsor del desarrollo empresarial. En lo metodológico, mediante el análisis de la literatura existente, se estudia el fenómeno desde la perspectiva teórica y, posteriormente, desde la perspectiva empírica, se hace uso de un análisis de framework sobre siete casos de estudio colombianos. De esta forma, se abordan conceptos relacionados con el desarrollo empresarial y la influencia de modelos de agronegocio de doble y triple impacto, así como en factores condicionantes para el otorgamiento de la figura de financiación respectiva. A manera de resultados, junto al modelo teórico expuesto, se suministra un planteamiento basado en cinco proposiciones de asociación, lo cual, prepara el terreno para su futura comprobación deductiva, tanto cualitativa como cuantitativamente.
ABSTRACT This work aims to determine the way in which social responsible investments has the potential of providing dynamism to the entrepreneurial development in agribusiness initiatives. At the same time, it seeks to integrate this concept with sustainable development objectives, and with conventional financial purposes within this particular industry. To do it, this work proposes an ideal theoretical model that exposes the dynamics of the social responsible investments and its positive influence on the entrepreneurial development. Methodologically, by using relevant literature, a conceptual analysis is performed from a theoretical perspective, followed by a framework analysis through the lens of seven Colombian case studies in order to perform a theory-building approach. Specifically, this work examines several concepts related with entrepreneurial development and the influence of double- and triple-bottom-line agribusiness models, together with conditioning factors for obtaining responsible financing. Together with the model at issue, this work provides a duly supported conceptual approach with five associative propositions, which ultimately fosters the prospective testing of the model trough deductive methods in both ways: qualitative and quantitative.
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El quilotórax es una enfermedad grave, más aún si es bilateral y de causa maligna, pero tiene tratamiento y el enfermo puede estabilizarse.Objetivo: presentar dos pacientes de interés médico y demostrar que puede esperarse buena respuesta al tratamiento.Casos Clínicos: se presentaron dos enfermos portadores de quilotórax bilateral y linfoma, ambos varones, atendidos en el hospital de forma simultánea; se realizaron los exámenes complementarios diagnósticos. El diagnóstico en ambos resultó linfoma no Hodgkin y Hodgkin respectivamente, fueron tratados con dieta, se restringieron las grasas; tratamiento oncoespecÍfico y toracocentésis repetidas en ambos hemitórax. El primero, mejorado del derrame bilateral y síntomas inicialmente, fallece a los 11 meses por no control de su enfermedad base. El segundo, estable de ambas afecciones a los 18 meses, en ambos casos el quilotórax se compensó y no fue necesario repetir drenaje. Se hacen análisis comparativo con otros reportes sobre todo cuando el linfoma es la causa etiológica, la conducta adoptada concuerda con otros estudios actuales.Conclusiones: los enfermos mejoraron del quilotórax bilateral, uno no estabilizó su enfermedad de base y murió, mientras el otro está asintomático. Puede obtenerse buena respuesta terapéutica...
Chylothorax is a serious disease, even more if it is bilateral and of a malignant cause; but it has treatment and the patients condition may be stable.Objective: to present two patients of medical interest and prove that a good response from the treatment may be expected.Clinical cases: two male patients with bilateral chylothorax and lymphoma were simultaneously attended at the hospital. Complementary diagnostic tests were made. The diagnosis of both patients turned out to be non-Hodgkins and Hodgkins lymphomas respectively. They were put on a diet in which fat was cut; the oncospecific treatment and repeated thoracocentesis were applied in both hemithoraxes. The first patient, after getting better from the bilateral effusion and initial symptoms, passed away 11 months later for not controlling the base disease. The second patient presented a stable condition of both complaints 18 months later. In both cases chylotorax was eased and it was not necessary to repeat the drainage. Analyses compared to other reports are made mainly when the lymphoma is the etiological cause; the adopted conduct coincides with other current studies.Conclusions: the patients got better from bilateral chylothorax; one of them did not get a stable condition of his base disease and died and the second one is asymptomatic. A good therapeutic response may be obtained...
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Masculino , Derrame Pleural , Linfoma , Quilotórax/diagnóstico , Relatos de CasosRESUMO
The solid-pseudopapillary tumors of the pancreas (TSPP) are a uncommon neoplasm (1- 2% of exocrine tumors). Are more frequent in young women and presents a low malignant potential (15% develop metastases). Its preoperative diagnosis is very difficult, because these tumors haven`t radiological features that makes distinguish from the other pancreatic cysts tumors. Usually are a histopathological finding. Surgical therapy provides good survival, even in the presence of metastases. We present 3 cases with clinical, imaging, treatment and histopathology to provide more information about this rare disease.
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Neoplasias Pancreáticas , Adulto , Feminino , Humanos , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/cirurgia , Adulto JovemRESUMO
Se evaluó el efecto de la suplementación de lisina en dietas para cerdos en iniciación sobre ganancia de peso y características de la canal y cárnicas. Se utilizaron 18 cerdos castrados (Yorkshire, Hampshire, Duroc y Landrace) con peso vivo inicial promedio de 10,13 kg y 40 días de edad. Se asignaron en tres tratamientos (T) con 6 repeticiones cada uno. El testigo (T1) fue dieta basal sin lisina adicionada; T2 = T1 + 0,285 kg de L-lisina/100 kg de alimento, y T3 = T1 + 0,569 kg de lisina/100 kg de alimento. Los cerdos se alimentaron por 33 d. La dieta basal fue de sorgo, (Sorghum vulgare) y pasta de soya, (Glicine max) (20,81% de PC y 3.460 Mcal EM/kg de MS). Al final del período de alimentación se registró el peso de los animales, la altura a la cruz (cm), circunferencia torácica (cm), espesor de grasa dorsal medida con ultrasonido. Para las características de la canal y cárnicas se sacrificaron al azar cuatro animales por tratamiento. Para muestras de costilla, jamón y paleta de cada canal se analizó para MS, PC, grasa y cenizas. No se encontró diferencias (P³0,05) entre tratamientos para ganancia de peso, altura a la cruz, circunferencia torácica, y grasa dorsal (mm). Para peso al sacrificio, rendimiento de la canal caliente y fría, área del ojo de la costilla, contenido de MS, PC, grasa y cenizas de costilla, jamón y paleta fueron similares (P³0,05) entre tratamientos. Se concluye que la lisina como promotor de crecimiento de tejido magro no se reflejó en ganancia de peso, características de la canal o composición de la carne de los cerdos en iniciación.
The effect of lysine supplementation in diets for growing pigs on weight gain, carcass and meat characteristics was evaluated. Eighteen castrated pigs (Yorkshire, Hampshire, Duroc y Landrace) of 10.13 kg initial weight and 40 d of T2 = T1 + 0.285 kg of lysine / 100 kg of feed; and T3 = T1 + 0.569100 kg of feed. Basal diet contained 20.81% CP and 3,460 Mcal/kg of ME. Pigs were fed for 33 d. At the end of the feeding trial final weight, cross height (cm) and thoracic circumference (cm), and subcutaneous fat measured with ultrasound were recorded. For carcass and meat characteristics, four pigs per treatment selected at random were slaughtered. Samples of rib, ham and shoulder from each carcass were analyzed for DM, CP, fat and ash. There were no differences (P>0.05) between treatments for weight gain, cross height, thoracic circumference, weight at sacrifice, hot and cold carcass yields, rib eye area and subcutaneous fat. The content of DM, CP, fat and ash was similar (P>0.05) among treatments. It is concluded that lysine as growth promotant of lean tissue was no reflected in weight gain, carcass characteristics or meat composition of the growing pigs.
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[reaction: see text] Aromatic O-triisopropylsilyl ketoximes were efficiently rearranged to cyclic and acyclic aniline derivatives on reduction with BF3-ethearate/borane. The bulk of the substituents on the silicon atom, the size of the aliphatic ring, and the presence of alkoxy substituents on the aryl group all play an important role in the aniline.
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Compostos de Anilina/síntese química , Boranos/química , Oximas/química , Compostos de Anilina/química , Estrutura Molecular , Oxirredução , EstereoisomerismoRESUMO
Statistical analysis of amino acid patterns in approximately 160,000 alpha-helices in experimentally determined structures revealed di-, tri-, and tetrapeptides, whose frequencies deviate most from the statistical model. Importantly, some sequences were never found in alpha- helices. This fact was detected initially with tripeptides, where nearly 1% of the possible sequences were never seen in the helical segments. For tetrapeptides, this effect is very strong and significant; almost 43% of the possible sequences never appear in alpha-helices. It is possible that there are some steric and energetic restrictions that do not allow these tetrameric amino acid sequences to form alpha-helical structure.
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Aminoácidos/químicaAssuntos
Humanos , Feminino , Adulto , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/terapia , Neoplasias da MamaAssuntos
Humanos , Feminino , Adulto , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/terapia , Neoplasias da MamaRESUMO
Objetivo. Presentar el reporte de un caso de infección congenita por Toxoplasma gondii, cuya manifestación clínica principal fue la presencia de dermatitis exfoliativa acompañada de hepatoesplenomegalia. Material y métodos. Se utiliza el diseño de reporte de caso, con una breve revisión de la literatura y discusión de los aspectos clínicos observados y su importancia en la medicina perinatal. Resultados. Se presentan las características clínicas de una infección congenita por Toxoplasma gondii que se manifesto como una dermatitis exfoliativa, asociada a hepato-esplenomegalia la dificultad diagnóstica que esta situación presento, el manejo médico establecido así como la evolución y seguimiento del paciente hasta los dos años de vida. Conclusiones. Se concluye que a pesar de que las manifestaciones cutáneas en el paciente con infección congénita por Toxoplasma gondii, son reportadas solo en forma esporádica, esta posibilidad debe ser incluída dentro del razonamiento diagnóstico en el paciente que se sospeche de infección congénita