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Rev. MVZ Córdoba ; 17(3): 3162-3168, set.-dic. 2012. ilus, tab
Artigo em Espanhol | LILACS, COLNAL | ID: lil-669279

RESUMO

Objetivo. Determinar los agentes infecciosos asociados causalmente a la presentación de Diarrea Neonatal Bovina (DNB) en terneros menores de 5 semanas de vida, procedentes de fincas lecheras y de producción mixta de la Sabana de Bogotá. Materiales y métodos. Se seleccionaron por conveniencia 21 fincas, se realizó seguimiento de 620 terneros desde el nacimiento hasta las 5 semanas de edad, se tomaron muestras de materia fecal de los animales que presentaron cuadro clínico de diarrea y de terneros clínicamente sanos como controles pareados. Se realizaron pruebas de ELISA para diagnóstico de E.coli F5, Rotavirus, Coronavirus, Cryptosporidium sp., y Salmonella sp., prueba de Ritchie para diagnóstico de Giardia sp., y tinción de Ziehl Neelsen modificada para Cryptosporidium sp. Se evaluó la asociación epidemiológica entre los agentes y la presentación de diarrea usando prueba de c2, seguido de un modelo de regresión logística (p<0.05). Resultados. Se encontró en la prueba de ELISA que de la totalidad de las muestras, 51 (38.3%), 26 (19.7%), 10 (7.5%) y 1 (0.75%) fueron positivas a Cryptosporidium sp., rotavirus, E coli F5 y coronavirus, respectivamente. Los animales positivos a Rotavirus por la prueba de ELISA y a Cryptosporidium sp., por la técnica de Ziehl Neelsen modificada tuvieron 2.6 y 7.0 veces mayor probabilidad que los demás animales de presentar DNB, respectivamente. Conclusiones. Los resultados presentados son los primeros que muestran el papel y la importancia del Cryptosporidium sp., y del Rotavirus en la DNB en las explotaciones ganaderas de la Sabana de Bogotá y en Colombia.


Objective. To determine the infectious agents causally associated to Bovine Neonatal Diarrhea (BND) in calves younger than five weeks of age from mixed production and dairy herds of Sabana de Bogota. Materials and methods. Twenty one herds were conveniently selected, and 620 calves were followed from birth up to 5 weeks of age, fecal samples were collected from animals with clinical signs of diarrhea and from calves without diarrhea which were taken as matched controls. ELISA tests were performed to diagnose E. coli F5, Rotavirus, Coronavirus, Salmonella sp, and Cryptosporidium sp, Ritchie test to diagnose Giardia sp., and a modified Ziehl Neelsen staining for Cryptosporidium sp. The epidemiological association between agents and the appearance of diarrhea was evaluated using c2 test, followed by a logistic regression model (p<0.05). Results. The ELISA test showed that 51 (38.3%), 26 (19.7%), 10 (7.5%) and 1 (0.75%) samples were positive for Cryptosporidium sp., rotavirus, E coli F5 and coronavirus, respectively. Animals that were positive for Rotavirus through ELISA and Cryptosporidium sp., through a modified Ziehl Neelsen technique had 2.6 and 7.0 times more probability than other animals to present BND, respectively. Conclusions. The results presented are the first to show the importance of Rotavirus and Cryptosporidium sp., in BND in the Sabana de Bogotá herds and in Colombia.


Assuntos
Diarreia , Cryptosporidium , Ensaio de Imunoadsorção Enzimática , Rotavirus
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