RESUMO
Background: Pasteurellosis is a common disease of cattle, pigs, and poultry, which rarely affects humans. In rabbits, therespiratory presentation of the disease is frequently reported. Clinical signs related to bronchopneumonia include sneezing, lung stertors, oculonasal discharge, dyspnea and cyanosis. Infection may lead to otitis, conjunctivitis, abscesses andsepsis. Furthermore, Pasteurella multocida infection may lead to sudden death without clinical manifestations. Reports ofpasteurellosis in rabbits are scarce in Brazil. Therefore, the objective of this article is to describe an outbreak of pasteurellosis with high mortality in a rabbity in the State of Santa Catarina, Brazil.Cases: Two adult rabbits were submitted for necropsy at the Veterinary Pathology Laboratory of the Instituto Federal Catarinense- Campus Concórdia, within an interval of twenty days. Herd was represented by 40 animals, of which six fattening rabbits andthree breeders died. Animals were kept in suspended cages with slatted floor. Clinical signs were represented by prostration,sneezing, and mucopurulent nasal discharge. In addition, wounds were observed in the distal portion of the limbs. Death occurredup to two days after the onset of clinical signs. Necropsies were performed and tissue samples were collected for histopathologic,immunohistochemical and microbiologic (bacterial culture and antibiogram) exams. At the necropsy, severe diffuse fibrinousexudate covering the pericardium sac, visceral and parietal pleural surfaces was noted, as well as multiple diaphragm adhesions.In addition, the lungs presented diffuse red coloration and showed multiple abscesses ranging from 0.3 to 1cm in diameter. Thenasal sinus and the tracheal mucosa showed diffuse reddening (rabbits 1 and 2). Abscesses up to 2 cm in diameter were observedin the mammary glands (rabbit 1), heart and kidneys (rabbit 2). The urinary bladder...(AU)
Assuntos
Animais , Coelhos , Infecções por Pasteurella/epidemiologia , Infecções por Pasteurella/patologia , Infecções por Pasteurella/veterinária , Pasteurella multocida/isolamento & purificação , Lagomorpha/virologia , Broncopneumonia/veterinária , Doenças Respiratórias/veterináriaRESUMO
Background: Pasteurellosis is a common disease of cattle, pigs, and poultry, which rarely affects humans. In rabbits, therespiratory presentation of the disease is frequently reported. Clinical signs related to bronchopneumonia include sneezing, lung stertors, oculonasal discharge, dyspnea and cyanosis. Infection may lead to otitis, conjunctivitis, abscesses andsepsis. Furthermore, Pasteurella multocida infection may lead to sudden death without clinical manifestations. Reports ofpasteurellosis in rabbits are scarce in Brazil. Therefore, the objective of this article is to describe an outbreak of pasteurellosis with high mortality in a rabbity in the State of Santa Catarina, Brazil.Cases: Two adult rabbits were submitted for necropsy at the Veterinary Pathology Laboratory of the Instituto Federal Catarinense- Campus Concórdia, within an interval of twenty days. Herd was represented by 40 animals, of which six fattening rabbits andthree breeders died. Animals were kept in suspended cages with slatted floor. Clinical signs were represented by prostration,sneezing, and mucopurulent nasal discharge. In addition, wounds were observed in the distal portion of the limbs. Death occurredup to two days after the onset of clinical signs. Necropsies were performed and tissue samples were collected for histopathologic,immunohistochemical and microbiologic (bacterial culture and antibiogram) exams. At the necropsy, severe diffuse fibrinousexudate covering the pericardium sac, visceral and parietal pleural surfaces was noted, as well as multiple diaphragm adhesions.In addition, the lungs presented diffuse red coloration and showed multiple abscesses ranging from 0.3 to 1cm in diameter. Thenasal sinus and the tracheal mucosa showed diffuse reddening (rabbits 1 and 2). Abscesses up to 2 cm in diameter were observedin the mammary glands (rabbit 1), heart and kidneys (rabbit 2). The urinary bladder...
Assuntos
Animais , Coelhos , Infecções por Pasteurella/epidemiologia , Infecções por Pasteurella/patologia , Infecções por Pasteurella/veterinária , Lagomorpha/virologia , Pasteurella multocida/isolamento & purificação , Broncopneumonia/veterinária , Doenças Respiratórias/veterináriaRESUMO
BACKGROUND: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). RESULTS: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. CONCLUSIONS: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2.
Assuntos
Infecções por Pasteurella/veterinária , Pasteurella multocida/genética , Pasteurella multocida/patogenicidade , Doenças dos Suínos/microbiologia , Animais , Brasil , Broncopneumonia/microbiologia , Broncopneumonia/veterinária , Genes Bacterianos , Tipagem de Sequências Multilocus/veterinária , Infecções por Pasteurella/genética , Pasteurella multocida/isolamento & purificação , Pericardite/microbiologia , Pericardite/veterinária , Pleurisia/microbiologia , Pleurisia/veterinária , Reação em Cadeia da Polimerase/veterinária , Suínos , Virulência/genéticaRESUMO
This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8 percent, considering titers >64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.
No presente trabalho, objetivou-se avaliar a soroprevalência da infecção por Toxoplasma gondii, em 708 matrizes suínas dos municípios de Nova Mutum e Diamantino do Estado de Mato Grosso, Brasil. As amostras de soro foram examinadas por meio da reação de imunofluorescência indireta (RIFI). Foi encontrada a frequência de 12,8 por cento de soros positivos, com diluições iguais ou superiores a 64. Portanto, os dados obtidos reforçam a necessidade de um manejo de criação adequado, visando à minimização do risco de infecção de suínos por T. gondii.
Assuntos
Animais , Doenças dos Suínos/sangue , Doenças dos Suínos/epidemiologia , Toxoplasma/imunologia , Toxoplasmose Animal/sangue , Toxoplasmose Animal/epidemiologia , Brasil/epidemiologia , Estudos Soroepidemiológicos , Suínos , Doenças dos Suínos/parasitologiaRESUMO
Zygomycosis is an important granulomatous disease that affects humans and animals, particularly sheep in tropical regions. Rhinofacial and nasopharyngeal zygomycosis were described in sheep in association with Conidiobolus spp. The present study characterized 5 samples of Conidiobolus isolated from 3 herds with clinical disease in Mato Grosso State, Brazil. The clinical and pathological findings were similar to nasopharyngeal zygomycosis. Based on morphological features, isolates were classified as Conidiobolus spp., and molecular phylogenetic analyses based on 18S ribosomal DNA grouped all isolates in a Conidiobolus lamprauges cluster. The current report describes the molecular characterization of ovine nasopharyngeal zygomycosis associated with C. lamprauges.
Assuntos
Doenças dos Ovinos/microbiologia , Zigomicose/veterinária , Animais , Brasil/epidemiologia , Conidiobolus/genética , Conidiobolus/isolamento & purificação , Filogenia , RNA Fúngico/genética , RNA Ribossômico 18S/genética , Ovinos , Doenças dos Ovinos/epidemiologia , Zigomicose/epidemiologia , Zigomicose/microbiologiaRESUMO
Streptococcus suis é um patógeno que a afeta a produção industrial de suínos em todo o mundo. É de extrema importância, pois está associado a doenças em suínos e humanos. O objetivo deste estudo foi determinar a prevalência do Streptococcus suis tipo 2 em 201 amostras de tonsilas de animais clinicamente sadios a partir da técnica de PCR. As amostras positivas foram submetidas à pesquisa do gene codificador do fator extracelular (ef). Os resultados demonstraram que a prevalência (23,38 por cento) foi maior que em outro estudo recentemente realizado no mesmo Estado, indicando que a PCR é um método mais sensível em relação ao isolamento bacteriano. Houve baixa ocorrência do gene ef* (1,49 por cento), o que mostra uma grande importância para população analisada, pois cepas negativas são potencialmente menos virulentas que cepas positivas.
Streptococcus suis is a pathogen that affects the industrial production of swine worldwide. It is extremely important, because it is associated with pigs and humans diseases. The aim of this study was to determine the prevalence of Streptococcus suis type 2 in 201 samples of tonsils from clinically healthy animals by the PCR technique. The samples positive for S. suis type 2 were tested for the gene encoding extracellular factors (ef). The results showed that the prevalence (23.38 percent) was higher than other recent survey in the State, demonstrating that the PCR is a more sensitive method in relation to the bacterial isolation. There was a low occurrence of ef* gene in samples (1.49 percent) showing great importance to local swine population, because negative strains are potentially less virulent that positive strains.