RESUMO
BACKGROUND: Adequate thyroid function is essential for normal growth and development of the fetus. Sonographic recognition of alterations in fetal thyroid dimensions may be the first sign of thyroid dysfunction, permitting early diagnosis and intervention. The main goal of this study was to build curves with reference values for ultrasound measurements of the fetal thyroid from 14 to 40 weeks of gestation. METHODS: This is a prospective longitudinal study of 90 Brazilian pregnant women, complementary to a cohort multicentre study named "WHO multicentre study for the development of growth standards from fetal life to childhood: the fetal component." Pregnant women without any pre-existing conditions that might affect fetal growth received antenatal care from the first trimester until childbirth, undergoing serial ultrasound evaluations of the fetus, including the thyroid. Longitudinal, anteroposterior, and transverse diameters of both thyroid lobes were measured in the fetus. Fetal thyroid lobe volume was also estimated. By quantile regression analysis, reference curves of measurements were fitted according to the gestational age. RESULTS: A reference standard of thyroid growth was defined during pregnancy by fitting curves of its measurements. Reference values for the 10th, 50th, and 90th centiles of fetal thyroid measurements (longitudinal, anteroposterior, transverse diameters, and lobe volume) were defined, from 14 to 40 weeks of gestation. CONCLUSION: We provided a reference curve of optimal thyroid development in a low-risk population that can be used as a standard of comparison to diagnose deviations from the norm. In addition, we demonstrated an alternative and simplified method for early recognition of thyroid morphological alterations by an individualized technique to evaluate the thyroid lobes.
Assuntos
Feto/diagnóstico por imagem , Glândula Tireoide/diagnóstico por imagem , Adolescente , Adulto , Brasil , Estudos de Coortes , Feminino , Desenvolvimento Fetal/fisiologia , Idade Gestacional , Humanos , Estudos Longitudinais , Gravidez , Cuidado Pré-Natal/métodos , Estudos Prospectivos , Valores de Referência , Doenças da Glândula Tireoide/diagnóstico , Doenças da Glândula Tireoide/diagnóstico por imagem , Ultrassonografia Pré-Natal/métodos , Adulto JovemRESUMO
BACKGROUND: Diabetes related distress is common in type 1 diabetes patients (T1D). High levels of diabetes distress are related to poor metabolic control. An instrument to evaluate diabetes distress in T1D patients is "type 1 diabetes scale-T1DDS". The aim of this study was to translate and culturally adapt the T1DDS into Brazilian culture. METHODS: T1DDS scale was translated into Portuguese. Back translation was performed and evaluated by a specialists committee. Pre-test was performed with 40 T1D outpatients at State University of Campinas hospital. Internal consistency, external consistency and re-test were performed. RESULTS: 72% women, mean age: 32, 1 ± 9, 7 years, mean diabetes duration: 15, 8 ± 9, 1 years, mean scholarity: 11, 5 ± 3, 6, glycosylated hemoglobin mean: 9 ± 2%. Internal consistency: Cronbach alpha of T1DDS Brazilian version was 0.93. External consistency: Spearman's coefficient between T1DDS and PAID, Brazilian version, was 0.7781; (p < 0.0001). CONCLUSIONS: The T1DDS Brazilian version is a reliable tool to evaluate diabetes distress in T1D patients in the Brazilian Population. This tool can be useful in clinical care and to identify patiens at risk and in need for psychosocial intervention.
RESUMO
BACKGROUND: Studies suggest that the ingestion of fish oil (FO), a source of the omega-3 polyunsaturated fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), can reduce the deleterious side-effects of chemotherapy. The aim of this randomised clinical trial was to evaluate the effect of supplementation with oral FO for 9 weeks on nutritional parameters and inflammatory nutritional risk in patients with haematological malignancies during the beginning of chemotherapy. METHODS: Twenty-two patients with leukaemia or lymphoma were randomised to the unsupplemented group (UG) (n = 13) or supplemented group (SG) (n = 9). SG received 2 g/day of fish oil for 9 weeks. Nutritional status, serum acute-phase proteins and plasma fatty acids were evaluated before (T0) and after (T1) the intervention period. Data were analysed using two models; model 1, comprising data from all patients included in the study, and model 2, comprising data from UG patients with no increase in the proportions of EPA and DHA in plasma and data from SG patients showing an at least 100% increase in plasma EPA and DHA. RESULTS: SG showed an increased plasma proportion of EPA and DHA in both models. In model 2, C-reactive protein (CRP) and CRP/albumin ratio showed larger reductions in the SG. Overall long-term survival in both models (465 days after the start of the chemotherapy) was higher in the group ingesting fish oil (P < 0.05). CONCLUSIONS: These findings indicate an improved nutritional-inflammatory risk and potential effects on long-term survival in patients with haematological malignancies supplemented with FO during the beginning of chemotherapy.
Assuntos
Suplementos Nutricionais , Óleos de Peixe/administração & dosagem , Neoplasias Hematológicas/tratamento farmacológico , Adolescente , Adulto , Idoso , Antropometria , Proteína C-Reativa/metabolismo , Ácidos Docosa-Hexaenoicos/administração & dosagem , Ácidos Docosa-Hexaenoicos/sangue , Ácido Eicosapentaenoico/administração & dosagem , Ácido Eicosapentaenoico/sangue , Feminino , Neoplasias Hematológicas/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Estado Nutricional , Albumina Sérica/metabolismo , Adulto JovemRESUMO
O presente trabalho visou avaliar a influência de diferentes tempos de extração no teor e composição química do óleo essencial da espécie Pothomorphe umbellata. Folhas de pariparoba foram coletadas em Cajamar - SP, no mês de junho de 2006. O delineamento experimental adotado foi inteiramente casualizado, com seis tempos de extração (90, 120, 150, 180, 210 e 240 minutos) e 4 repetições. A extração do óleo essencial foi realizada por hidrodestilação e a análise química em CG-EM. O tempo de 180 minutos proporcionou maior rendimento do óleo essencial (0,42%), enquanto a composição química não foi influenciada pelos diferentes tempos de destilação. As principais substâncias identificadas foram: D-germacreno (65,5%), alfa-selineno (9,7%), trans-cariofileno (7,5%), e espatulenol (6,58%). Conclui-se que o tempo de extração afeta diretamente o rendimento e a proporção das substâncias químicas, recomendando-se a extração em até 180 minutos. .
The present work aimed to evaluate the influence of different times of extraction in the yield and chemical composition of the essential oil of "pariparoba" (Pothomorphe umbellate). Leaves of "pariparoba" were collected in the municipal district of Cajamar - state of São Paulo, Brazil, in June 2006. The adopted experimental design was completely randomized, with six times of extraction (90, 120, 150, 180, 210, and 240 minutes) and 4 repetitions. The extraction of the essential oil was accomplished by hydrodistillation, and the chemical analysis was performed in GC-MS. The time of extraction of 180 minutes provided greater yield of the essential oil (0.42%), and its chemical composition was not influenced by the different times of hydrodistillation. The main substances showed are D-germacrene (65.5%), alpha-selinene (9.7%), trans-caryophyllene (7.5%) and spathulenol (6.58%). The results of this work showed that the time of extraction affects the yield and the proportion of the chemical substances and it is recommended the extraction in up to 180 minutes.
Assuntos
Óleos Voláteis/síntese química , Química , Piperaceae/anatomia & histologia , /análise , Destilação/instrumentaçãoRESUMO
No início dos anos 2000, o Brasil se consolidou como maior exportador mundial de carne de frango, mesmo período em que se observou a emergência global de focos de HPAI. Para assegurar a qualidade sanitária do produto avícola nacional, o Mapa organizou um programa oficial de vigilância para o vírus de IA. Na primeira fase, foram coletados 106.226 soros e 7.017 pools de suabes traqueais e cloacais, provenientes de granjas avícolas de produção comercial intensiva de frangos de corte, no período entre janeiro de 2004 e março de 2005. Não se obteve isolamento viral para IA, porém foi identificado um conglomerado epidemiológico de 24 municípios, delineado pela interpolação de dados relacionados às localizações geográficas, e os resultados sorológicos das amostras dessas origens, ELISA reagente para IA, no estado de Rondônia. Na segunda fase da vigilância, três distintas ações para pesquisa do vírus de IA foram executadas entre 2006 e 2007, em: 1) aves comerciais de corte de criação intensiva; 2) aves de reprodução; e 3) aves migratórias e de subsistência. Não houve identificação de resposta sorológica ou isolamento de vírus de IA em aves dos grupos 1 e 2. Foram isolados vírus de IA do subtipo H3 em aves migratórias, capturadas nos estados de Pará e Pernambuco. Também foram identificados vírus de IA dos subtipos H2, H3 e H4 em aves de subsistência, de propriedades localizadas no Amazonas, Pará, Pernambuco, Rio Grande do Sul e Santa Catarina. Observou-se risco sanitário para LPAI associado às populações de aves silvestres e de subsistência localizadas em áreas próximas ao sistema comercial. Estudos adicionais serão necessários para se avaliar o risco associado à introdução de IA no sistema comercial avícola brasileiro.
In the early 2000s Brazil had established itself as the world's largest exporter of poultry meat, the same period in which the emergence of HPAI global outbreaks was observed. To ensure the national health quality of Brazilian poultry product, MAPA organized an official AI surveillance program. In the first stage, during the period between January 2004 and March 2005, 106.226 sera were collected and 7.017 tracheal and cloacae pools of swabs were obtained from intensive commercial broiler farms. No AI virus isolation was obtained, however, an epidemiological cluster was identified in the state of Rondonia, outlined by the interpolation of data related to municipalities' geographic location and serological response to AI in ELISA tests. Between 2006 and 2007, during the second stage, three AI surveillance actions were executed in: 1) intensive commercial broiler farms, 2) breeding farms and 3) migratory and backyard birds. There was neither serological response identification nor IA virus isolation in birds belonging to groups 1 and 2. H3 LPAI subtype viruses were isolated from migratory birds captured in the states of Pará and Pernambuco. H2, H3 and H4 LPAI subtypes were also identified in backyard birds from samples collected in the states of Amazonas, Pará, Pernambuco, Rio Grande do Sul and Santa Catarina. There are health risks to LPAI associated to wild and backyard bird populations located in areas close to commercial farms. Additional studies are needed for risk assessment regarding the possibility of AI introduction in the Brazilian commercial poultry system.
Assuntos
Animais , Aves Domésticas/análise , Aves Domésticas/métodos , Ensaio de Imunoadsorção Enzimática , Ensaio de Imunoadsorção Enzimática/veterinária , Influenza Aviária/epidemiologiaRESUMO
No início dos anos 2000, o Brasil se consolidou como maior exportador mundial de carne de frango, mesmo período em que se observou a emergência global de focos de HPAI. Para assegurar a qualidade sanitária do produto avícola nacional, o Mapa organizou um programa oficial de vigilância para o vírus de IA. Na primeira fase, foram coletados 106.226 soros e 7.017 pools de suabes traqueais e cloacais, provenientes de granjas avícolas de produção comercial intensiva de frangos de corte, no período entre janeiro de 2004 e março de 2005. Não se obteve isolamento viral para IA, porém foi identificado um conglomerado epidemiológico de 24 municípios, delineado pela interpolação de dados relacionados às localizações geográficas, e os resultados sorológicos das amostras dessas origens, ELISA reagente para IA, no estado de Rondônia. Na segunda fase da vigilância, três distintas ações para pesquisa do vírus de IA foram executadas entre 2006 e 2007, em: 1) aves comerciais de corte de criação intensiva; 2) aves de reprodução; e 3) aves migratórias e de subsistência. Não houve identificação de resposta sorológica ou isolamento de vírus de IA em aves dos grupos 1 e 2. Foram isolados vírus de IA do subtipo H3 em aves migratórias, capturadas nos estados de Pará e Pernambuco. Também foram identificados vírus de IA dos subtipos H2, H3 e H4 em aves de subsistência, de propriedades localizadas no Amazonas, Pará, Pernambuco, Rio Grande do Sul e Santa Catarina. Observou-se risco sanitário para LPAI associado às populações de aves silvestres e de subsistência localizadas em áreas próximas ao sistema comercial. Estudos adicionais serão necessários para se avaliar o risco associado à introdução de IA no sistema comercial avícola brasileiro.(AU)
In the early 2000s Brazil had established itself as the world's largest exporter of poultry meat, the same period in which the emergence of HPAI global outbreaks was observed. To ensure the national health quality of Brazilian poultry product, MAPA organized an official AI surveillance program. In the first stage, during the period between January 2004 and March 2005, 106.226 sera were collected and 7.017 tracheal and cloacae pools of swabs were obtained from intensive commercial broiler farms. No AI virus isolation was obtained, however, an epidemiological cluster was identified in the state of Rondonia, outlined by the interpolation of data related to municipalities' geographic location and serological response to AI in ELISA tests. Between 2006 and 2007, during the second stage, three AI surveillance actions were executed in: 1) intensive commercial broiler farms, 2) breeding farms and 3) migratory and backyard birds. There was neither serological response identification nor IA virus isolation in birds belonging to groups 1 and 2. H3 LPAI subtype viruses were isolated from migratory birds captured in the states of Pará and Pernambuco. H2, H3 and H4 LPAI subtypes were also identified in backyard birds from samples collected in the states of Amazonas, Pará, Pernambuco, Rio Grande do Sul and Santa Catarina. There are health risks to LPAI associated to wild and backyard bird populations located in areas close to commercial farms. Additional studies are needed for risk assessment regarding the possibility of AI introduction in the Brazilian commercial poultry system.(AU)
Assuntos
Animais , Ensaio de Imunoadsorção Enzimática , Ensaio de Imunoadsorção Enzimática/veterinária , Aves Domésticas/análise , Aves Domésticas/métodos , Influenza Aviária/epidemiologiaRESUMO
Spiders are exceptionally diverse and abundant organisms in terrestrial ecosystems and their evolutionary success is certainly related to their capacity to produce different types of silks during their life cycle, making a specialized use on each of them. Presenting particularly tandemly arranged amino acid repeats, silk proteins (spidroins) have mechanical properties superior to most synthetic or natural high-performance fibers, which makes them very promising for biotechnology industry, with putative applications in the production of new biomaterials. During the evolution of spider species, complex behaviors of web production and usage have been coupled with anatomical specialization of spinning glands. Spiders retaining ancestral characters, such as the ones belonging to the Mygalomorph group, present simpler sorts of webs used mainly to build burrows and egg sacs, and their silks are produced by globular undifferentiated spinning glands. In contrast, Araneomorphae spiders have a complex spinning apparatus, presenting up to seven morphologically distinct glands, capable to produce a more complex set of silk polymers with different degrees of rigidness and elasticity associated with distinct behaviors. Aiming to provide a discussion involving a number of spider silks' biological aspects, in this review we present descriptions of members from each family of spidroin identified from five spider species of the Brazilian biodiversity, and an evolutionary study of them in correlation with the anatomical specialization of glands and spider's spinning behaviors. Due to the biotechnological importance of spider silks for the production of new biomaterials, we also discuss about the new possible technical and biomedical applications of spider silks and the current status of it.
Assuntos
Biotecnologia , Fibroínas/química , Fibroínas/metabolismo , Família Multigênica , Sequência de Aminoácidos , Animais , Fenômenos Biomecânicos , Evolução Molecular , Fibroínas/ultraestrutura , Dados de Sequência MolecularRESUMO
This paper aims to present an ergonomic device to assist in the maintenance of the units of Tucuruí Hydropower Plant. The development of this ergonomic device made possible to reduce maintenance time, reduce losses caused by billing, improve performance and reduce the physical strain for labors during the execution of services.
Assuntos
Ergonomia/instrumentação , Centrais Elétricas , Gestão de Riscos , Análise e Desempenho de Tarefas , Gestão da Qualidade Total , Brasil , Monitoramento Ambiental , Ergonomia/economia , Humanos , Doenças Profissionais/prevenção & controle , Saúde Ocupacional/normas , Esforço Físico , Fatores de TempoRESUMO
A Pothomorphe umbellata (Piperaceae), é uma espécie medicinal nativa do Brasil, utilizada na indústria de cosméticos e protetores de pele contra raios UVA e UVB. Com o intuito de gerar informações aplicadas à propagação da espécie, o presente trabalho relacionou a coloração e a massa de sementes de P. umbellata a seu comportamento germinativo. A coloração e a massa de sementes de P. umbellata foram características adequadas para avaliar a homogeneidade fisiológica, o vigor, o potencial e o comportamento germinativo. Assim, conclui-se que, embora de germinação lenta, as sementes de coloração preta e mais densa devem ser as escolhidas quando de coleta ou de processo seletivo.
Pothomorphe umbellata (Piperaceae) is a medicinal species native to Brazil, which has been used in the cosmetic industry and in products that protect the skin against UVA and UVB rays. To generate information applied to the species propagation, the present work related the coloration and the mass of P. umbellata seeds to their germinative behavior. The coloration and the mass of seeds of P. umbellata were characteristic appropriate to evaluate physiologic homogeneity, vigor, potential and germinative behavior. Thus, although of slow germination, seeds of black and denser coloration should be chosen during collection or selective process.
Assuntos
Pigmentação/genética , Piperaceae/embriologia , Sementes/anatomia & histologia , Plantas Medicinais/embriologiaRESUMO
Therapy with bone marrow-derived cells has been used in ischemic patients with reported success. The aim of this study was to determine the therapeutic efficacy of fresh and frozen human umbilical cord blood cells (hUCB) in Wistar rats submitted to permanent occlusion of the left coronary artery. Three hours after myocardial infarction, 2 x 10(7) hUCB cells or vehicle were administered by intramyocardial injection. The animals were divided into five groups: control (N = 10), sham operated (N = 10), infarcted that received vehicle (N = 9), infarcted treated with cryopreserved hUCB (N = 7), and infarcted treated with fresh hUCB (N = 5). Cardiac function was evaluated by electrocardiogram (ECG) and echocardiogram (ECHO) before cell therapy, and by ECG, ECHO, cardiopulmonary test, and left ventricular pressure measurements 3 weeks later. After 3 weeks, both groups treated with hUCB still had Q wave present in L1, âQRS >90 degrees and reduced shortening fraction (less than 50%). In addition, cardiac indexes of left ventricular contractility and relaxation were 5484 +/- 875 and -4032 +/- 643 mmHg (cryopreserved hUCB) and 4585 +/- 955 and -2862 +/- 590 mmHg (fresh hUCB), respectively. These values were not statistically different from those of saline-treated animals. Cardiopulmonary exercise test profile was typical of infarcted hearts; exercise time was about 14 min and maximal VO2 was 24.77 +/- 5.00 mL.kg-1.min-1. These data show that hUCB therapy did not improve the cardiac function of infarcted animals or prevent cardiac remodeling.
Assuntos
Transplante de Células-Tronco de Sangue do Cordão Umbilical , Infarto do Miocárdio/cirurgia , Animais , Ecocardiografia , Eletrocardiografia , Humanos , Infarto do Miocárdio/fisiopatologia , Ratos , Ratos Wistar , Função Ventricular Esquerda/fisiologiaRESUMO
Therapy with bone marrow-derived cells has been used in ischemic patients with reported success. The aim of this study was to determine the therapeutic efficacy of fresh and frozen human umbilical cord blood cells (hUCB) in Wistar rats submitted to permanent occlusion of the left coronary artery. Three hours after myocardial infarction, 2 x 10(7) hUCB cells or vehicle were administered by intramyocardial injection. The animals were divided into five groups: control (N = 10), sham operated (N = 10), infarcted that received vehicle (N = 9), infarcted treated with cryopreserved hUCB (N = 7), and infarcted treated with fresh hUCB (N = 5). Cardiac function was evaluated by electrocardiogram (ECG) and echocardiogram (ECHO) before cell therapy, and by ECG, ECHO, cardiopulmonary test, and left ventricular pressure measurements 3 weeks later. After 3 weeks, both groups treated with hUCB still had Q wave present in L1, âQRS >90° and reduced shortening fraction (less than 50 percent). In addition, cardiac indexes of left ventricular contractility and relaxation were 5484 ± 875 and -4032 ± 643 mmHg (cryopreserved hUCB) and 4585 ± 955 and -2862 ± 590 mmHg (fresh hUCB), respectively. These values were not statistically different from those of saline-treated animals. Cardiopulmonary exercise test profile was typical of infarcted hearts; exercise time was about 14 min and maximal VO2 was 24.77 ± 5.00 mL·kg-1·min-1. These data show that hUCB therapy did not improve the cardiac function of infarcted animals or prevent cardiac remodeling.
Assuntos
Animais , Humanos , Ratos , Transplante de Células-Tronco de Sangue do Cordão Umbilical , Infarto do Miocárdio/cirurgia , Ecocardiografia , Eletrocardiografia , Infarto do Miocárdio/fisiopatologia , Ratos Wistar , Função Ventricular Esquerda/fisiologiaRESUMO
Oxytocin is well known for its role in reproduction. However, evidence has emerged suggesting a role in cardiovascular and hydroelectrolytic homeostasis. Although its renal effects have been characterized, the cardiac ones have not been much studied. Therefore, we aimed to investigate the cardiac effects of oxytocin both in vivo and in vitro. In unanesthetized rats (n=6) intravenous oxytocin (1 mug) decreased dP/dt(max) by 15% (P<0.05) and heart rate by 20% (P<0.001), at the first minute after injection. dP/dt(max) was still lower in OT-treated rats than in controls (n=8) after 15 min (P<0.05), while heart rate returned to control values after 5 min. In isolated hearts, oxytocin was able to promote negative inotropic and chronotropic effects. Perfusion with 10(-5), 10(-6) and 10(-7)M oxytocin resulted in approximately 60% (P<0.01), 25% (P<0.01) and 10% (P<0.05) reduction of left ventricle developed pressure, without effect in lower concentrations (10(-10) to 10(-8) M). Also, dP/dt(max) was reduced by 45 and 20% (10(-5) e 10(-6) M; P<0.01), while diastolic pressure raised and heart rate fell only with 10(-5)M oxytocin (P<0.05). Intravenous oxytocin (1 mug; n=6) increased arterial pressure by 22% at the first minute (+23+/-3 mm Hg; P<0.001), returning to control value thereafter. Thus, oxytocin is able to promote directly negative inotropic and chronotropic effects, but its in vivo effect also involves a reflex mechanism, originated from its pressor effect.
Assuntos
Coração/efeitos dos fármacos , Contração Miocárdica/efeitos dos fármacos , Ocitocina/farmacologia , Animais , Pressão Sanguínea/efeitos dos fármacos , Depressão Química , Coração/fisiologia , Frequência Cardíaca/efeitos dos fármacos , Homeostase/efeitos dos fármacos , Masculino , Ratos , Ratos WistarRESUMO
The aim of the present study was to evaluate the effect of amiodarone on mean arterial pressure (MAP), heart rate (HR), baroreflex, Bezold-Jarisch, and peripheral chemoreflex in normotensive and chronic one-kidney, one-clip (1K1C) hypertensive rats (N = 9 to 11 rats in each group). Amiodarone (50 mg/kg, iv) elicited hypotension and bradycardia in normotensive (-10 +/- 1 mmHg, -57 +/- 6 bpm) and hypertensive rats (-37 +/- 7 mmHg, -39 +/- 19 bpm). The baroreflex index (deltaHR/deltaMAP) was significantly attenuated by amiodarone in both normotensive (-0.61 +/- 0.12 vs -1.47 +/- 0.14 bpm/mmHg for reflex bradycardia and -1.15 +/- 0.19 vs -2.63 +/- 0.26 bpm/mmHg for reflex tachycardia) and hypertensive rats (-0.26 +/- 0.05 vs -0.72 +/- 0.16 bpm/mmHg for reflex bradycardia and -0.92 +/- 0.19 vs -1.51 +/- 0.19 bpm/mmHg for reflex tachycardia). The slope of linear regression from delta pulse interval/deltaMAP was attenuated for both reflex bradycardia and tachycardia in normotensive rats (-0.47 +/- 0.13 vs -0.94 +/- 0.19 ms/mmHg and -0.80 +/- 0.13 vs -1.11 +/- 0.13 ms/mmHg), but only for reflex bradycardia in hypertensive rats (-0.15 +/- 0.02 vs -0.23 +/- 0.3 ms/mmHg). In addition, the MAP and HR responses to the Bezold-Jarisch reflex were 20-30% smaller in amiodarone-treated normotensive or hypertensive rats. The bradycardic response to peripheral chemoreflex activation with intravenous potassium cyanide was also attenuated by amiodarone in both normotensive (-30 +/- 6 vs -49 +/- 8 bpm) and hypertensive rats (-34 +/- 13 vs -42 +/- 10 bpm). On the basis of the well-known electrophysiological effects of amiodarone, the sinus node might be the responsible for the attenuation of the cardiovascular reflexes found in the present study.
Assuntos
Amiodarona/farmacologia , Antiarrítmicos/farmacologia , Células Quimiorreceptoras/efeitos dos fármacos , Hemodinâmica/efeitos dos fármacos , Hipertensão Renal/fisiopatologia , Animais , Barorreflexo/efeitos dos fármacos , Pressão Sanguínea/efeitos dos fármacos , Doença Crônica , Eletrofisiologia , Frequência Cardíaca/efeitos dos fármacos , Masculino , Ratos , Ratos WistarRESUMO
The aim of the present study was to evaluate the effect of amiodarone on mean arterial pressure (MAP), heart rate (HR), baroreflex, Bezold-Jarisch, and peripheral chemoreflex in normotensive and chronic one-kidney, one-clip (1K1C) hypertensive rats (N = 9 to 11 rats in each group). Amiodarone (50 mg/kg, iv) elicited hypotension and bradycardia in normotensive (-10 ± 1 mmHg, -57 ± 6 bpm) and hypertensive rats (-37 ± 7 mmHg, -39 ± 19 bpm). The baroreflex index (deltaHR/deltaMAP) was significantly attenuated by amiodarone in both normotensive (-0.61 ± 0.12 vs -1.47 ± 0.14 bpm/mmHg for reflex bradycardia and -1.15 ± 0.19 vs -2.63 ± 0.26 bpm/mmHg for reflex tachycardia) and hypertensive rats (-0.26 ± 0.05 vs -0.72 ± 0.16 bpm/mmHg for reflex bradycardia and -0.92 ± 0.19 vs -1.51 ± 0.19 bpm/mmHg for reflex tachycardia). The slope of linear regression from deltapulse interval/deltaMAP was attenuated for both reflex bradycardia and tachycardia in normotensive rats (-0.47 ± 0.13 vs -0.94 ± 0.19 ms/mmHg and -0.80 ± 0.13 vs -1.11 ± 0.13 ms/mmHg), but only for reflex bradycardia in hypertensive rats (-0.15 ± 0.02 vs -0.23 ± 0.3 ms/mmHg). In addition, the MAP and HR responses to the Bezold-Jarisch reflex were 20-30 percent smaller in amiodarone-treated normotensive or hypertensive rats. The bradycardic response to peripheral chemoreflex activation with intravenous potassium cyanide was also attenuated by amiodarone in both normotensive (-30 ± 6 vs -49 ± 8 bpm) and hypertensive rats (-34 ± 13 vs -42 ± 10 bpm). On the basis of the well-known electrophysiological effects of amiodarone, the sinus node might be the responsible for the attenuation of the cardiovascular reflexes found in the present study.
Assuntos
Animais , Masculino , Ratos , Amiodarona/farmacologia , Antiarrítmicos/farmacologia , Células Quimiorreceptoras , Hemodinâmica/efeitos dos fármacos , Hipertensão Renal/fisiopatologia , Barorreflexo/efeitos dos fármacos , Pressão Sanguínea/efeitos dos fármacos , Doença Crônica , Eletrofisiologia , Frequência Cardíaca/efeitos dos fármacos , Ratos WistarRESUMO
To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related individuals until the second generation, derived from kennels located in the states of Minas Gerais and Säo Paulo. The CMR S primer was used for the Polimerase Chain Reaction (PCR). The results showed the inefficiency of the technique, even when analyzed through the automated gel analysis system. Also showed the impossibility of its commercial use due to the fact of does not permit the storage of data for subsequent use
Assuntos
Desoxirribonucleases , CãesRESUMO
Foram utilizados 46 animais da raça Gir, registrados na Associaçäo Brasileira de Criadores de Zebu, provenientes de cinco fazendas situadas no Estado de Minas Gerais, com o objetivo de avaliar a eficiência dos microssatélites BM2113, ILSTS005, ILSTS008, ETH131 e RM88 em testes de verificaçäo de parentesco. Os locos BM2113, ILSTS005, ETH131 e RM88 mostraram-se eficientes, apresentando valores de PE2 (probabilidade de exclusäo quando os dois progenitores säo genotipados) entre 0,62 e 0,69 e PIC2 (conteúdo de informaçäo polimórfica quando os dois progenitores säo genotipados) entre 0,78 e 0,83. O mesmo näo ocorreu para o loco ILSTS008, o qual apresentou baixos valores de PE2 (0,24) e PIC2 (0,41)
Assuntos
Bovinos , DNARESUMO
Foram utilizados 46 animais da raça Gir, registrados na Associação Brasileira de Criadores de Zebu, provenientes de cinco fazendas situadas no Estado de Minas Gerais, com o objetivo de avaliar a eficiência dos microssatélites BM2113, ILSTS005, ILSTS008, ETH131 e RM88 em testes de verificação de parentesco. Os locos BM2113, ILSTS005, ETH131 e RM88 mostraram-se eficientes, apresentando valores de PE2 (probabilidade de exclusão quando os dois progenitores são genotipados) entre 0,62 e 0,69 e PIC2 (conteúdo de informação polimórfica quando os dois progenitores são genotipados) entre 0,78 e 0,83. O mesmo não ocorreu para o loco ILSTS008, o qual apresentou baixos valores de PE2 (0,24) e PIC2 (0,41).(AU)
Forty six animals of the Gir breed, registered at the Brazilian Association of Zebu Breeders, coming from five farms located in Minas Gerais State, were used to analyze the efficiency of the microsatellites BM2113, ILSTS005, ILSTS008, ETH131 and RM88 in parentage tests. The loci BM2113, ILSTS005, ETH131 and RM88 showed to be efficient, presenting values of PE2 (exclusion probability when both parents are genotiped) between 0.62 and 0.69 and PIC2 (polymorphic information contents when both parents are genotiped) between 0.78 and 0.83. The same was not observed for the locus ILSTS008 that showed low values of PE2 (0.24) and PIC2 (0.41).(AU)
Assuntos
Animais , Bovinos , DNARESUMO
To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related individuals until the second generation, derived from kennels located in the states of Minas Gerais and São Paulo. The CMR S primer was used for the Polimerase Chain Reaction (PCR). The results showed the inefficiency of the technique, even when analyzed through the automated gel analysis system. Also showed the impossibility of its commercial use due to the fact of does not permit the storage of data for subsequent use.
RESUMO
OBJECTIVE: To analyze the effects of in-hospital reocclusion of reperfused AMI culprit coronary arteries in mortality and to identify the predictors. METHODS: The present study comprises a sample of 155 patients with AMI who underwent successful mechanical reperfusion by direct coronary angioplasty and angiographic control during hospitalization or before discharge. Patients were classified into group A: reoccluded patients (n=30) and group B: non-reoccluded patients (n=125). RESULTS: We identified in-hospital reocclusion predictors and found a greater significance in mortality among reoccluded patients (23,3% x 1.6%; p=0.00004). Silent reocclusion or typical angina at reocclusion had a good prognosis. The independent predictors of in-hospital mortality were hypertension, multiarterial lesions, totally occluded AMI culprit lesions, failed redilatation, failed redilatation in comparison with no intention to redilate, no redilatation in comparison with no attempt to redilate, and reocclusion within the first 48 to 72 hours. The decision to redilate, independently of the result, led to a 50.0% reduction in hospital mortality (p=0.0366). CONCLUSION: In-hospital AMI culprit coronary artery reocclusion had an adverse effect similar to that reported in clinical studies with high mortality rates (23.3% x 1.6%; p=0.00004). The major contribution of this study is to recommend the reopening of reoccluded AMI culprit coronary arteries as a means for the management of coronary artery reocclusion.