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BACKGROUND: Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome. OBJECTIVES: In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. METHODS: DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture. RESULTS: In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations. CONCLUSION: Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection.
Assuntos
Leishmania braziliensis , Leishmania , Leishmaniose Cutânea , Humanos , Leishmania braziliensis/metabolismo , Leishmaniose Cutânea/parasitologia , Moléculas de Adesão Celular/metabolismoRESUMO
BACKGROUND Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome. OBJECTIVES In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. METHODS DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture. RESULTS In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations. CONCLUSION Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection.
RESUMO
To investigate whether low-level laser therapy (LLLT), at different times of application (immediate and late) in the region of the parotid glands, has a distance effect on the microarchitecture of the trabecular bone in mandible of rats irradiated by volumetric modular arc therapy (VMAT). Thirty adult Wistar rats were randomly divided into placebo control groups (CG, n = 2), only radiotherapy (RG, n = 2), only LPLT (LG, n = 2), and two other groups using LLLT in the immediate time (24 h) (ILG, n = 12) and late (120 h) (LLG, n = 12) to radiotherapy by VMAT in a single dose of 12 Gy. LLLT with AsGaAl laser (660 nm, 100 mW), a spot size of 0.0028 cm2, was applied in three points in the region of the right parotid gland, with energy of 2 J/cm2, 20 s per point, for 10 consecutive days. After euthanasia, the right hemimandibles of each animal were dissected, prepared, and analyzed by computerized microtomography (micro-CT) and histomorphometry. The different groups were analyzed by the Tukey and Bonferroni multiple comparison tests. The micro-CT analysis found statistically significant differences between the groups, especially in the LLG, which had the highest average bone volume compared to the CG (p = 0.001) and ILG (p = 0.002) and a greater number of trabeculae than the CG (p = 0.000) and ILG (p = 0.031). The ILG also had a higher number of trabeculae than the CG (p = 0.005). Trabecula separation (Tb.Sp) was lower in the LLG (p = 0.000) and ILG (p = 0.002) when compared to the CG. In the histomorphometry, there was no statistical difference between the groups in relation to all the analyzed variables. Micro-CT analysis showed that the LLLT, even applied at a distance, both in the immediate and late VMAT times, has an effect on the mandibular bone microarchitecture by increasing the volume and number of trabeculae and decreasing the spaces between them.
Assuntos
Terapia com Luz de Baixa Intensidade , Animais , Osso Esponjoso , Terapia com Luz de Baixa Intensidade/métodos , Mandíbula/diagnóstico por imagem , Ratos , Ratos Wistar , Microtomografia por Raio-XRESUMO
A Lei nº 13.168, de 6 de outubro de 2015, determina entre outros aspectos, que as Instituições de Ensino Superior (IES) brasileiras tragam em seus sítios eletrônicosoficiaisinformações relativas àmatrizcurricular e corpo docente do curso ofertado. O objetivo deste estudo foi avaliar a prevalência de cumprimento dessa lei em IES que ofertam o curso de Odontologia, tendo como amostra IES das regiões Sudeste e Sul do país. Para isso, as IES foram catalogadasa partir doportal e-MEC do Ministério da Educação, e posteriormentetiveram seus sítios eletrônicos oficiais acessados. Observou-seentão o cumprimento das determinações do artigo, parágrafo, incisos, e alíneas da referida lei. Os dados foram descritos por meio defrequências absolutas e relativas e comparados pelo Teste Exato de Fisher (α=5%). Aplicados os critérios de elegibilidade, 256 IES foram incluídas, sendo 220 IES privadas e 36 públicas. De acordo com os critérios de análise adotados, apenas 3 (1,17%) das IES cumpriram todas as exigências da lei. A disponibilização da lista das disciplinas que compõem a matriz curricular de cada curso foi de 78,1%. Por outro lado, informações relativas ao corpo docente foram disponibilizadas por uma baixa porcentagem de IES, tais como identificação do corpo docente (40,6%), as disciplinas que efetivamente cada docente leciona (13,7%), titulação (30,1%) e tempo de trabalho na IES (8,6%). Do mesmo modo, uma baixa porcentagem foi observada quanto àatualização (11,7%) e data completa da última atualização (14,8%). Em conclusão, a maior parte das IES analisadas não está de acordo com Lei nº 13.168/15, sendo necessário que os coordenadores de curso e gestores estejam atentosà necessidade de adequação dos sítios eletrônicos institucionais (AU).
Law No. 13,168, of October 6, 2015, determines, among other things, that Brazilian Higher Education Institutions (HEIs) bring on their official websites information regarding the curriculum and teaching staff of the course offered. The aim of this study was to assess the prevalence of compliance with this law in HEIs that offer the Dentistry course, using HEIs from the Southeast and South regions of the country as a sample. For this, the HEIs were cataloged on the Ministry of Education's e-MEC portal, and later had their official websites accessed. It was observed whether the HEI portals complied with the provisions of the article, paragraph, items, and paragraphs of the aforementioned law. Data were described using absolute and relative frequencies and compared using Fisher's exact test at a significance level of 5%. After applying the eligibility criteria, 256 HEIs were included, being 220 private HEIs and 36 public. According to the analysis criteria adopted, only 3 (1.17%) of the HEIs complied with all the requirements of the law. The availability of the list of subjects that make up the curriculum of each course was 78.1%. On the other hand, information related to the teaching staff was made available by a low percentage of HEIs, such as identification ofthe teaching staff (40.6%), the subjects that each professor actually teaches (13.7%), titles (30.1 %), and working time at the HEI (8.6%). Likewise, a low percentage was observed regarding the updating of the curriculum (11.7%) and the presence of the complete date of the last update (14.8%). In conclusion, most Brazilian Dentistry HEIs do not comply with Law No. 13.168/15, making it necessary for course coordinators and education managers to pay attention to the need to adapt institutional websites (AU).
Assuntos
Universidades , Aplicação da Lei/ética , Educação em Odontologia , Brasil , Estudos Transversais/métodos , Interpretação Estatística de Dados , Currículo , Mídias Sociais/éticaRESUMO
Introduction: This study aimed to investigate the effect of low-level laser therapy (LLLT) on the blood cell count when applied to parotid glands of rats irradiated by volumetric modular arc therapy (VMAT). Methods: Thirty-two adult male Wistar rats were used in this study. Samples were randomly assigned to three groups: control group (CG, n = 8), immediate laser group (24 hours) (ILG, n=12), and late laser group (120 hours) (LLG, n=12). The two laser groups were previously subjected to VMAT radiotherapy in a single dose of 12 Gy. LLLT with an AsGaAl laser (660 nm, 100 mW) was applied at three points in the region of the parotid glands, right side, with the energy of 2 J per point (20s, 70 J/cm2) and a spot size of 0.0028 cm2 for 10 consecutive days. In the euthanasia, blood samples were obtained by cardiac puncture. The samples from each group were processed by an automatic method and analyzed for erythrogram, leukogram and platelet count values. The data were analyzed by ANOVA and each LLLT time point was analyzed in relation to the control group, with a significance level less than 0.05. Results: Groups using LLLT had higher red blood cell counts, being higher in the LLG (P = 0.000). The hematimetric indices MCV (P = 0.002) and MCH (P = 0.009) were lower than the control group, especially when compared to the group using LLLT 120h after radiotherapy (LLG). White blood cell counts were lower in the groups with radiotherapy and immediate use of LLLT (ILG) (P = 0.011), mainly at the expense of lymphocytes (P = 0.002). Conclusion: The results suggest a potential systemic effect of LLLT, especially on circulating red blood cell counts, regardless of their time of immediate or late use of radiotherapy.
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The aim of this study was to investigate the occurrence, type and severity of anemia at the time of diagnosis of oral cancer, and its potential association with the degree of tumor cell differentiation. This case-control study used 366 medical records of patients treated at two referral centers for oral cancer diagnosis, specifically: cases (n=70) with a histopathological diagnosis of oral squamous cell carcinoma (OSCC) of the oral cavity, and controls (n=296) with benign oral lesions. Sociodemographic, behavioral, and clinical variables of both groups, as well as complete blood count values, were analyzed by descriptive statistics and crude/adjusted logistic regression. Anemia was detected in 15.7% of the cases and 11.8% of the controls. The presence of anemia had an OR=1.64 (odds ratio) (95%CI 0.54-5.00) for OSCC, with no significantly statistical association. Normocytic anemia was the most prevalent form of anemia when oral cancer was diagnosed (91.4% of the controls and 72.7% of the cases), and moderate to severely low hemoglobin levels were associated with OSCC diagnosis (OR 6.49; 95%CI 1.18-35.24), albeit data on hematological examinations were missing.
Assuntos
Anemia , Carcinoma de Células Escamosas , Neoplasias de Cabeça e Pescoço , Neoplasias Bucais , Anemia/epidemiologia , Anemia/etiologia , Carcinoma de Células Escamosas/epidemiologia , Estudos de Casos e Controles , Humanos , Neoplasias Bucais/epidemiologiaRESUMO
Tropical forests are experiencing unprecedented high-temperature conditions due to climate change that could limit their photosynthetic functions. We studied the high-temperature sensitivity of photosynthesis in a rainforest site in southern Amazonia, where some of the highest temperatures and most rapid warming in the Tropics have been recorded. The quantum yield (Fv /Fm ) of photosystem II was measured in seven dominant tree species using leaf discs exposed to varying levels of heat stress. T50 was calculated as the temperature at which Fv /Fm was half the maximum value. T5 is defined as the breakpoint temperature, at which Fv /Fm decline was initiated. Leaf thermotolerance in the rapidly warming southern Amazonia was the highest recorded for forest tree species globally. T50 and T5 varied between species, with one mid-storey species, Amaioua guianensis, exhibiting particularly high T50 and T5 values. While the T50 values of the species sampled were several degrees above the maximum air temperatures experienced in southern Amazonia, the T5 values of several species are now exceeded under present-day maximum air temperatures.
Assuntos
Mudança Climática , Fotossíntese/fisiologia , Folhas de Planta/fisiologia , Termotolerância/fisiologia , Árvores/fisiologia , Brasil , Complexo de Proteína do Fotossistema II/metabolismo , Floresta ÚmidaRESUMO
Abstract The aim of this study was to investigate the occurrence, type and severity of anemia at the time of diagnosis of oral cancer, and its potential association with the degree of tumor cell differentiation. This case-control study used 366 medical records of patients treated at two referral centers for oral cancer diagnosis, specifically: cases (n=70) with a histopathological diagnosis of oral squamous cell carcinoma (OSCC) of the oral cavity, and controls (n=296) with benign oral lesions. Sociodemographic, behavioral, and clinical variables of both groups, as well as complete blood count values, were analyzed by descriptive statistics and crude/adjusted logistic regression. Anemia was detected in 15.7% of the cases and 11.8% of the controls. The presence of anemia had an OR=1.64 (odds ratio) (95%CI 0.54-5.00) for OSCC, with no significantly statistical association. Normocytic anemia was the most prevalent form of anemia when oral cancer was diagnosed (91.4% of the controls and 72.7% of the cases), and moderate to severely low hemoglobin levels were associated with OSCC diagnosis (OR 6.49; 95%CI 1.18-35.24), albeit data on hematological examinations were missing.
Assuntos
Humanos , Neoplasias Bucais/epidemiologia , Carcinoma de Células Escamosas/epidemiologia , Neoplasias de Cabeça e Pescoço , Anemia/etiologia , Anemia/epidemiologia , Estudos de Casos e ControlesRESUMO
Introduction: This experimental study investigated the mRNA expression of aquaporins (AQPs) 1 and 5 in the parotid glands of rats irradiated with volumetric modulated arc therapy (VMAT) and subjected to low-level laser therapy (LLLT) at different time points. Methods: The sample consisted of 30 Wistar rats (Rattus norvegicus) divided into the following groups: control, LLLT alone (LG), radiotherapy alone (RG), and experimental groups that received LLLT at 24 hours (early experimental group [EEG], n=12) and 120 hours (late experimental group [LEG], n=12) after radiotherapy. VMAT was delivered at a single dose (12 Gy) and LLLT was performed with an aluminium-gallium-arsenide diode laser (660 nm, 100 mW), spot area of 0.0028 cm2, energy of 2 J/cm2 applied to 3 spots in the region corresponding to the right parotid gland, for 10 consecutive days. The right parotid gland was resected and prepared for RNA extraction. The gene expression of AQPs was evaluated by quantitative polymerase chain reaction (qPCR) using specific TaqMan probes, with the HPRT gene as an internal control. Results: The lowest AQP1 gene expression was 0.83 (0.27) with the use of LLLT 24 hours after radiotherapy (EEG), and the highest was 1.56 (0.80) with the use of LLLT alone (LG). Likewise, the lowest AQP5 gene expression was found in the EEG (mean = 0.88; SD = 0.49) and the highest in the LG (mean = 1.29; SD = 0.33). Conclusion: The use of LLLT after radiotherapy may contribute to the maintenance and an increase of these proteins, even when used at a later time point after radiotherapy.
RESUMO
Salmonella Infantis is frequently associated with human infections worldwide and is transmitted by consumption of contaminated foods, particularly those of animal origin, especially the chicken meat. We aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated from samples of poultry origin. The strains were isolated from 2009 to 2010 in a company with full cycle of broiler's production in the state of São Paulo, Brazil. The antimicrobial susceptibility test was performed and, by PCR, we evaluated the presence of the genes lpfA (hem-adhesion), agfA (hem-biofilm) and sefA (hem-adhesion) and resistance genes to beta-lactams (blaTEM, blaSHV, bla CTX-M and blaAmpC ). The phylogenetic relationship was determined by RAPD-PCR method. Among the drugs tested, the highest percentages of resistance were to amoxicillin (35.3%) and to sulfonamide (15.7%). Eleven antimicrobial resistance patterns were identified (A1 to A11), none of them presented a multiresistance profile (> 3 antimicrobials classes). There was 100% of positivity for the agfA gene, 92.2% for the lpfA gene, and no strain presented the sefA gene. Most of the isolates showed similarities in virulence potential, since they were simultaneously positive for two studied genes, agfA and lpfA (92.2%, 47/51). Of the 18 (35.3%) strains resistant to antimicrobials of the β-lactam class, 10 (55.5%) were positive to blaAmpC gene, five (27.8%) for blaCTX-M , two (11.1%) to blaSHV and no strain presented the blaTEM gene. The phylogenetic evaluation has shown the presence of five clusters (A, B, C, D and E) with similarity greatSalmonella Infantis is frequently associated with human infections worldwide and is transmitted by consumption of contaminated foods, particularly those of animal origin, especially the chicken meat. We aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated from samples of poultry origin. The strains were isolated from 2009 to 2010 in a company with full cycle of broiler's production in the state of São Paulo, Brazil. The antimicrobial susceptibility test was performed and, by PCR, we evaluated the presence of the genes lpfA (hem-adhesion), agfA (hem-biofilm) and sefA (hem-adhesion) and resistance genes to beta-lactams (blaTEM, blaSHV, bla CTX-M and blaAmpC ). The phylogenetic relationship was determined by RAPD-PCR method. Among the drugs tested, the highest percentages of resistance were to amoxicillin (35.3%) and to sulfonamide (15.7%). Eleven antimicrobial resistance patterns were identified (A1 to A11), none of them presented a multiresistance profile (> 3 antimicrobials classes). There was 100% of positivity for the agfA gene, 92.2% for the lpfA gene, and no strain presented the sefA gene. Most of the isolates showed similarities in virulence potential, since they were simultaneously positive for two studied genes, agfA and lpfA (92.2%, 47/51). Of the 18 (35.3%) strains resistant to antimicrobials of the ß-lactam class, 10 (55.5%) were positive to blaAmpC gene, five (27.8%) for blaCTX-M , two (11.1%) to blaSHV and no strain presented the blaTEM gene. The phylogenetic evaluation has shown the presence of five clusters (A, B, C, D and E) with similarity greater than 80%, and three distinct strains which were not grouped in any cluster. Cluster B grouped 33 strains, all positive for lpfA and agfA genes, from both, the broiler farming facility and the slaughterhouse, persistent throughout all the study period. This cluster also grouped 18 strains clones with genetic similarity greater than 99%, all isolated in the slaughterhouse. The presence of virulence genes associated with persistent strains clones for a long period, warns to the possibility of S. Infantis to form biofilm, and should be constantly monitored in broilers' production chain, in order to know the profile of the strains that may contaminate the final product and evaluate the hazards that represents to public health.er than 80%, and three distinct strains which were not grouped in any cluster. Cluster B grouped 33 strains, all positive for lpfA and agfA genes, from both, the broiler farming facility and the slaughterhouse, persistent throughout all the study period. This cluster also grouped 18 strains clones with genetic similarity greater than 99%, all isolated in the slaughterhouse. The presence of virulence genes associated with persistent strains clones for a long period, warns to the possibility of S. Infantis to form biofilm, and should be constantly monitored in broilers' production chain, in order to know the profile of the strains that may contaminate the final product and evaluate the hazards that represents to public health.(AU)
Salmonella Infantis é frequentemente associada a infecções humanas no mundo todo sendo transmitida pelo consumo de alimentos contaminados, principalmente aqueles de origem animal, com destaque para a carne de frango. Objetivou-se avaliar características de virulência, resistência antimicrobiana e a similaridade genética de 51 estirpes de S. Infantis isoladas em amostras de origem avícola. As estirpes foram isoladas no período de 2009 a 2010 em uma empresa com ciclo completo de produção de frango de corte, localizada no estado de São Paulo, Brasil. Foi realizado o teste de susceptibilidade antimicrobiana e pela técnica de PCR, foi avaliada a presença dos genes lpfA (fímbria-adesão), agfA (fímbria-biofilme) e sefA (fímbria-adesão) e os genes de resistência aos beta-lactâmicos (bla TEM, blaSHV, blaCTX-M e blaAmpC ). A relação filogenética foi determinada pelo método de RAPD-PCR. Dentre as drogas testadas, os maiores percentuais de resistência foram para amoxacilina com 35,3% e sulfonamida com 15,7%. Onze perfis de resistência aos antimicrobianos foram identificados (A1 a A11), sendo que nenhum deles apresentou perfil de multirresistência (>3 classes de antimicrobianos). Houve 100% de positividade para o gene agfA, 92,2% para o gene lpfA e nenhuma estirpe apresentou o gene sefA. A maioria dos isolados apresentaram semelhanças no potencial de virulência, pois foram positivos simultaneamente para dois genes estudados, agfA e lpfA (92,2% - 47/51). Das 18 (35,3%) estirpes resistentes aos antimicrobianos da classe dos ß-lactâmicos, 10 (55,5%) foram positivas para o gene blaAmpC , cinco (27,8%) para blaCTX-M , duas (11,1%) para blaSHV e nenhuma estirpe apresentou o gene bla TEM . A avaliação filogenética demonstrou a presença de cinco clusters (A, B, C, D e E) com similaridade superior a 80%, e três estirpes distintas que não foram agrupadas em nenhum dos clusters. O cluster B agrupou 33 estirpes, todas positivas para os genes lpfA e agfA, provenientes tanto do aviário quanto do matadouro frigorífico, persistentes durante todo o período do estudo. Este cluster ainda agrupou 18 estirpes clones com similaridade genética superior a 99%, todas isoladas no matadouro frigorífico. A presença dos genes de virulência, associada à persistência das estirpes clones durante um longo período do estudo, alertam para a possibilidade de S. Infantis em formar biofilme, devendo ser constantemente monitorada na cadeia de produção avícola, especialmente no ambiente de abate, de forma a conhecer o perfil das estirpes que podem contaminar o produto final e assim avaliar os perigos que representam para a saúde pública.(AU)
Assuntos
Animais , Salmonella/isolamento & purificação , Salmonella/genética , Salmonella/patogenicidade , Salmonelose Animal , Resistência Microbiana a Medicamentos/genética , Galinhas/microbiologia , beta-Lactamas , Amoxicilina , Infecções por SalmonellaRESUMO
Salmonella Infantis is frequently associated with human infections worldwide and is transmitted by consumption of contaminated foods, particularly those of animal origin, especially the chicken meat. We aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated from samples of poultry origin. The strains were isolated from 2009 to 2010 in a company with full cycle of broiler's production in the state of São Paulo, Brazil. The antimicrobial susceptibility test was performed and, by PCR, we evaluated the presence of the genes lpfA (hem-adhesion), agfA (hem-biofilm) and sefA (hem-adhesion) and resistance genes to beta-lactams (blaTEM, blaSHV, bla CTX-M and blaAmpC ). The phylogenetic relationship was determined by RAPD-PCR method. Among the drugs tested, the highest percentages of resistance were to amoxicillin (35.3%) and to sulfonamide (15.7%). Eleven antimicrobial resistance patterns were identified (A1 to A11), none of them presented a multiresistance profile (> 3 antimicrobials classes). There was 100% of positivity for the agfA gene, 92.2% for the lpfA gene, and no strain presented the sefA gene. Most of the isolates showed similarities in virulence potential, since they were simultaneously positive for two studied genes, agfA and lpfA (92.2%, 47/51). Of the 18 (35.3%) strains resistant to antimicrobials of the β-lactam class, 10 (55.5%) were positive to blaAmpC gene, five (27.8%) for blaCTX-M , two (11.1%) to blaSHV and no strain presented the blaTEM gene. The phylogenetic evaluation has shown the presence of five clusters (A, B, C, D and E) with similarity greatSalmonella Infantis is frequently associated with human infections worldwide and is transmitted by consumption of contaminated foods, particularly those of animal origin, especially the chicken meat...(AU)
Salmonella Infantis é frequentemente associada a infecções humanas no mundo todo sendo transmitida pelo consumo de alimentos contaminados, principalmente aqueles de origem animal, com destaque para a carne de frango. Objetivou-se avaliar características de virulência, resistência antimicrobiana e a similaridade genética de 51 estirpes de S. Infantis isoladas em amostras de origem avícola. As estirpes foram isoladas no período de 2009 a 2010 em uma empresa com ciclo completo de produção de frango de corte, localizada no estado de São Paulo, Brasil. Foi realizado o teste de susceptibilidade antimicrobiana e pela técnica de PCR, foi avaliada a presença dos genes lpfA (fímbria-adesão), agfA (fímbria-biofilme) e sefA (fímbria-adesão) e os genes de resistência aos beta-lactâmicos (bla TEM, blaSHV, blaCTX-M e blaAmpC ). A relação filogenética foi determinada pelo método de RAPD-PCR. Dentre as drogas testadas, os maiores percentuais de resistência foram para amoxacilina com 35,3% e sulfonamida com 15,7%. Onze perfis de resistência aos antimicrobianos foram identificados (A1 a A11), sendo que nenhum deles apresentou perfil de multirresistência (>3 classes de antimicrobianos). Houve 100% de positividade para o gene agfA, 92,2% para o gene lpfA e nenhuma estirpe apresentou o gene sefA. A maioria dos isolados apresentaram semelhanças no potencial de virulência, pois foram positivos simultaneamente para dois genes estudados, agfA e lpfA (92,2% - 47/51). Das 18 (35,3%) estirpes resistentes aos antimicrobianos da classe dos ß-lactâmicos, 10 (55,5%) foram positivas para o gene blaAmpC , cinco (27,8%) para blaCTX-M , duas (11,1%) para blaSHV e nenhuma estirpe apresentou o gene bla TEM . A avaliação filogenética demonstrou a presença de cinco clusters (A, B, C, D e E) com similaridade superior a 80%, e três estirpes distintas que não foram agrupadas em nenhum dos clusters...(AU)
Assuntos
Animais , Salmonella/isolamento & purificação , Salmonella/genética , Salmonella/patogenicidade , Salmonelose Animal , Resistência Microbiana a Medicamentos/genética , Galinhas/microbiologia , beta-Lactamas , Amoxicilina , Infecções por SalmonellaRESUMO
ABSTRACT: Salmonella Infantis is frequently associated with human infections worldwide and is transmitted by consumption of contaminated foods, particularly those of animal origin, especially the chicken meat. We aimed to evaluate virulence characteristics, antimicrobial resistance and the genetic similarity of 51 strains of S. Infantis isolated from samples of poultry origin. The strains were isolated from 2009 to 2010 in a company with full cycle of broilers production in the state of São Paulo, Brazil. The antimicrobial susceptibility test was performed and, by PCR, we evaluated the presence of the genes lpfA (hem-adhesion), agfA (hem-biofilm) and sefA (hem-adhesion) and resistance genes to beta-lactams (blaTEM, blaSHV, bla CTX-M and blaAmpC ). The phylogenetic relationship was determined by RAPD-PCR method. Among the drugs tested, the highest percentages of resistance were to amoxicillin (35.3%) and to sulfonamide (15.7%). Eleven antimicrobial resistance patterns were identified (A1 to A11), none of them presented a multiresistance profile (> 3 antimicrobials classes). There was 100% of positivity for the agfA gene, 92.2% for the lpfA gene, and no strain presented the sefA gene. Most of the isolates showed similarities in virulence potential, since they were simultaneously positive for two studied genes, agfA and lpfA (92.2%, 47/51). Of the 18 (35.3%) strains resistant to antimicrobials of the -lactam class, 10 (55.5%) were positive to blaAmpC gene, five (27.8%) for blaCTX-M , two (11.1%) to blaSHV and no strain presented the blaTEM gene. The phylogenetic evaluation has shown the presence of five clusters (A, B, C, D and E) with similarity greater than 80%, and three distinct strains which were not grouped in any cluster. Cluster B grouped 33 strains, all positive for lpfA and agfA genes, from both, the broiler farming facility and the slaughterhouse, persistent throughout all the study period. This cluster also grouped 18 strains clones with genetic similarity greater than 99%, all isolated in the slaughterhouse. The presence of virulence genes associated with persistent strains clones for a long period, warns to the possibility of S. Infantis to form biofilm, and should be constantly monitored in broilers production chain, in order to know the profile of the strains that may contaminate the final product and evaluate the hazards that represents to public health.
RESUMO: Salmonella Infantis é frequentemente associada a infecções humanas no mundo todo sendo transmitida pelo consumo de alimentos contaminados, principalmente aqueles de origem animal, com destaque para a carne de frango. Objetivou-se avaliar características de virulência, resistência antimicrobiana e a similaridade genética de 51 estirpes de S. Infantis isoladas em amostras de origem avícola. As estirpes foram isoladas no período de 2009 a 2010 em uma empresa com ciclo completo de produção de frango de corte, localizada no estado de São Paulo, Brasil. Foi realizado o teste de susceptibilidade antimicrobiana e pela técnica de PCR, foi avaliada a presença dos genes lpfA (fímbria-adesão), agfA (fímbria-biofilme) e sefA (fímbria-adesão) e os genes de resistência aos beta-lactâmicos (bla TEM, blaSHV, blaCTX-M e blaAmpC ). A relação filogenética foi determinada pelo método de RAPD-PCR. Dentre as drogas testadas, os maiores percentuais de resistência foram para amoxacilina com 35,3% e sulfonamida com 15,7%. Onze perfis de resistência aos antimicrobianos foram identificados (A1 a A11), sendo que nenhum deles apresentou perfil de multirresistência (>3 classes de antimicrobianos). Houve 100% de positividade para o gene agfA, 92,2% para o gene lpfA e nenhuma estirpe apresentou o gene sefA. A maioria dos isolados apresentaram semelhanças no potencial de virulência, pois foram positivos simultaneamente para dois genes estudados, agfA e lpfA (92,2% - 47/51). Das 18 (35,3%) estirpes resistentes aos antimicrobianos da classe dos -lactâmicos, 10 (55,5%) foram positivas para o gene blaAmpC , cinco (27,8%) para blaCTX-M , duas (11,1%) para blaSHV e nenhuma estirpe apresentou o gene bla TEM . A avaliação filogenética demonstrou a presença de cinco clusters (A, B, C, D e E) com similaridade superior a 80%, e três estirpes distintas que não foram agrupadas em nenhum dos clusters. O cluster B agrupou 33 estirpes, todas positivas para os genes lpfA e agfA, provenientes tanto do aviário quanto do matadouro frigorífico, persistentes durante todo o período do estudo. Este cluster ainda agrupou 18 estirpes clones com similaridade genética superior a 99%, todas isoladas no matadouro frigorífico. A presença dos genes de virulência, associada à persistência das estirpes clones durante um longo período do estudo, alertam para a possibilidade de S. Infantis em formar biofilme, devendo ser constantemente monitorada na cadeia de produção avícola, especialmente no ambiente de abate, de forma a conhecer o perfil das estirpes que podem contaminar o produto final e assim avaliar os perigos que representam para a saúde pública.
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ABSTRACT Objective to analyze the surface roughness and color stability of the composite resin after surface treatment with the aluminum oxide discs and subsequent exposure to the Advanced Teeth Whitening Strips. Methods 20 specimens of the Filtek Z350XT restorer (3M®) were prepared and daily subjected to home bleaching for 30 minutes during 14 days. A precision rugosimeter was used in order to verify the roughness by means of 3 random readings in the same direction in each sample. For color stability the CIELAB system (L*, a*, b* values) was used through the spectrophotometer (MINOLTA CR -321, Japan). The means of the test specimens as well as the mean of each group were calculated using the random readings. Data statistical analysis were performed by ANOVA- analysis of variance. The level of significance was set at 5% (p ≤ 0.05). Results Roughness did not present great numerical variations. No statistically significant difference between the means obtained concerning the surface roughness of the composite resin with p = 0.44 was observed. However, it was found that there was a statistically significant difference between the means obtained in relation to the color stability of the composite resin, p=0. 007. Conclusion It was concluded that pre-contoured strips containing 6% hydrogen peroxide do not have a significant adverse effect on the roughness of Z350XT (3M®) resin. However, it was also concluded that according to the color stability analysis performed, there may be an indication of the restoration replacement after the bleaching treatment, due to their color change.
RESUMO Objetivo Analisar a rugosidade superficial e a estabilidade da cor da resina composta após tratamento de superfície com os discos de óxido de alumínio e posterior exposição à fita clareadora Advanced Teeth Whitening Strips. Métodos Foram confeccionados 20 corpos de prova do restaurador universal Filtek Z350XT (3M®), estes, foram submetidos ao clareamento caseiro por 30 minutos diários durante 14 dias. Utilizou-se um rugosímetro de precisão para verificação da rugosidade através de 3 leituras aleatórias no mesmo sentido em cada amostra, e para estabilidade de cor foi utilizado o sistema CIELAB (valores L*, a*, b*), através do espectofotômetro (MINOLTA CR-321, Japão). As leituras coletadas possibilitaram o cálculo das médias dos corpos de prova e a média de cada grupo. Em seguida, os dados foram submetidos a tratamento estatístico pela análise de variância ANOVA. O nível de significância estabelecido foi de 5% (p≤0,05). Resultados a rugosidade não sofreu grandes variações numéricas e também não houve diferença estatisticamente significantes entre as médias obtidas em relação a rugosidade superficial da resina composta (p=0,44). Contudo, verificou-se que houve diferenças estatisticamente significantes entre as medias obtidas em relação à estabilidade de cor da resina composta, com p= 0,007. Conclusão As fitas pré-contornadas contendo peróxido de hidrogênio a 6% não provocam efeito adverso significantes sobre a rugosidade da resina Z350XT (3M®). Contudo, também concluímos que, de acordo com a análise de estabilidade de cor realizada, poderá haver indicação da substituição das restaurações após o tratamento clareador devido à alteração na coloração das mesmas.
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Schistosomiasis, caused by helminth flatworms of the genus Schistosoma, is one of the most important parasitic diseases in the world, affecting over 200 million people in developing countries. Riparins are natural alkamides found in Aniba riparia (Lauraceae) fruits that possess several pharmacological properties. In this study, we reported the synthesis, characterization and structural analysis of six riparin derivatives (A-F), as well as their schistosomicidal activity against S. mansoni worms together with a biological, pharmacokinetic and toxicological in silico evaluation. Firstly, these compounds were synthesized, purified and characterized by elemental analysis, FT-IR spectroscopy, X-ray diffraction and theoretical calculations to evaluate their stability and conformation. Next, the schistosomicidal activity of the riparins was tested against S. mansoni worms. Bioassays revealed that Riparins E and F were the most active compounds, showing half-maximum inhibitory concentration at low micromolar ranges (IC50 values ~10⯵M). Also, confocal laser scanning microscopy studies revealed tegumental damage in parasites after exposition with Riparins B, E and F. Additionally, based on MTT assay, all tested riparins showed no cytotoxic potential toward mammalian cells. Finally, in silico analyses were used to predict the absorption, distribution, metabolism, elimination and toxicity (ADMET) of the compounds. Taken together, the results revealed a promising ADMET profile and suggested that riparins could be starting points for lead optimization programs for natural products with antischistosomal properties.
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Benzamidas , Fenetilaminas , Esquistossomicidas , Animais , Benzamidas/química , Benzamidas/farmacocinética , Benzamidas/farmacologia , Benzamidas/toxicidade , Células CACO-2 , Sobrevivência Celular/efeitos dos fármacos , Chlorocebus aethiops , Simulação por Computador , Humanos , Absorção Intestinal , Modelos Biológicos , Estrutura Molecular , Fenetilaminas/química , Fenetilaminas/farmacocinética , Fenetilaminas/farmacologia , Fenetilaminas/toxicidade , Difração de Pó , Schistosoma mansoni/efeitos dos fármacos , Esquistossomicidas/química , Esquistossomicidas/farmacocinética , Esquistossomicidas/farmacologia , Esquistossomicidas/toxicidade , Absorção Cutânea , Espectroscopia de Infravermelho com Transformada de Fourier , Células Vero , Difração de Raios XRESUMO
A importância da helmintose em diferentes espécies de açougue se dá pelos prejuízos econômicos gerados pelas condenações de suas carcaças e vísceras. O presente estudo avaliou as condenações por migração larvar em fígados e pulmões em um frigorífico de equídeos sob inspeção federal localizado em Minas Gerais no período de janeiro a dezembro de 2016. Os 13.636 animais pesquisados eram provenientes dos Estados de Goiás, Pernambuco, Bahia e Piauí, sendo os dados obtidos por meio dos registros das condenações realizadas na inspeção post mortem de acordo com os critérios do serviço de inspeção federal. Dos fígados e pulmões condenados, 12% e 8% respectivamente corresponderam à condenação por migração larval, podendo concluir que o prejuízo devido à parasitose se toma evidente no abate de equinos, principalmente com o acometimento em maior porcentagem de fígados e com representatividade à ocorrência de parasitas também nos pulmões.(AU)
The importance of helminthoses in different species of butchers is due to the economic damages generated by the condemnation of their carcasses and viscera. The present study evaluated the condemnations for larval migration in livers and lungs in a equidae slaughterhouse under federal inspection located in Minas Gerais from January to December 2016. The 13,636 animals studied were from the States of Goiás, Pernambuco, Bahia and Piaui, the data being obtained through the records of the convictions carried out in the post-mortem inspection according to the criteria of the federal inspection service. Of the condemned livers and lungs, 12% and 8%, respectively, corresponded to the larval migration conviction, and it can be concluded that the parasitic injury is evident in the slaughter of horses, mainly with involvement in a higher percentage of livers, and with the occurrence of parasites also in the lungs.(AU)
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Animais , Helmintíase , Doenças dos Cavalos , Matadouros , Pulmão/parasitologia , Fígado/parasitologia , Cavalos , VíscerasRESUMO
A importância da helmintose em diferentes espécies de açougue se dá pelos prejuízos econômicos gerados pelas condenações de suas carcaças e vísceras. O presente estudo avaliou as condenações por migração larvar em fígados e pulmões em um frigorífico de equídeos sob inspeção federal localizado em Minas Gerais no período de janeiro a dezembro de 2016. Os 13.636 animais pesquisados eram provenientes dos Estados de Goiás, Pernambuco, Bahia e Piauí, sendo os dados obtidos por meio dos registros das condenações realizadas na inspeção post mortem de acordo com os critérios do serviço de inspeção federal. Dos fígados e pulmões condenados, 12% e 8% respectivamente corresponderam à condenação por migração larval, podendo concluir que o prejuízo devido à parasitose se torna evidente no abate de equinos, principalmente com o acometimento em maior porcentagem de fígados e com representatividade à ocorrência de parasitas também nos pulmões.(AU)
The importance of helminthoses in different species of butchers is due to the economic damages generated by the condemnation of their carcasses and viscera. The present study evaluated the condemnations for larval migration in livers and lungs in a equidae slaughterhouse under federal inspection located in Minas Gerais from January to December 2016. The 13,636 animals studied were from the States of Goiás, Pernambuco, Bahia and Piauí , the data being obtained through the records of the convictions carried out in the post-mortem inspection according to the criteria of the federal inspection service. Of the condemned livers and lungs, 12% and 8%, respectively, corresponded to the larval migration conviction, and it can be concluded that the parasitic injury is evident in the slaughter of horses, mainly with involvement in a higher percentage of livers, and with the occurrence of parasites also in the lungs.
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Animais , Contaminação de Alimentos/análise , Inspeção de Alimentos , Parasitologia de Alimentos , Equidae , Matadouros/normas , Carne/parasitologia , Helmintos/isolamento & purificação , Helmintos/parasitologia , CavalosRESUMO
Oral and maxillofacial (OMF) injuries in children may lead to developmental and functional disorders. In this cross-sectional study, we analyzed the pattern of OMF injuries in preschool children admitted to the emergency department of a hospital in southern Brazil. Subjects and method: We retrospectively reviewed the medical records of 1,533 patients admitted from January to December 2011 to identify children aged 0 to 5 years with a diagnosis of OMF trauma. Data were collected on demographic, seasonal and clinical variables. Results: The sample consisted of 288 preschool children (aged 0-5 years) with OMF injuries. Of these, 61.1% were male and 53.2% were <3 years of age. Most children (84.8%) received first medical care from an dentist. Isolated facial injuries were the most common type of injury (85.1%) in this age group. Soft tissue injuries were the most common injury outcome (77.9%), occurring mainly in the nose and lips. Random accidents were the most common cause of OMF trauma (87.7%), and injuries due to falls occurred in 85.9% of these cases. The most common dental trauma was tooth displacement (69.6%). Conclusion: In conclusion, our findings indicate a higher prevalence of OMF injuries in soft tissues, especially in boys in the first three years of life. Falls were the leading cause of injury. Information related to OMF injuries in children are essential for the control of accidents and development of public health policies.
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Cross-sectional study of 36 children with acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML). Intraoral examination and oral hygiene assessment were performed in all patients. Information was also obtained from interviews of patients' mothers and a review of medical records. Results: The sample was largely composed of boys (58.3%), ages 59 years. The most commonly used chemotherapy regimen was the BFM-95 protocol. Most children (83.3%) had a record of some oral manifestation during treatment. On intraoral examination, 17 children (50%) were found to have at least one oral manifestation. The most frequent manifestation was also mucositis (26.5%), followed by gingival bleeding (23.4%). Lower maternal educational level was associated with increased frequency of mucositis, as were induction chemotherapy and maintenance chemotherapy after relapse. Conclusions: Oral abnormalities were common in children receiving chemotherapy and mucositis was the most prevalent manifestation. Oral lesions were associated with the induction phase of chemotherapy. Mucositis was not associated with oral health status.
Estudo transversal de 36 crianças com leucemia linfoblástica aguda (LLA) ou leucemia mieloide aguda (LMA). Exame intraoral e avaliação de higiene bucal foram realizados em todos os pacientes. A informação também foi obtida a partir de entrevistas com as mães dos pacientes e uma revisão dos registros médicos. Resultados: A amostra foi composta em grande parte dos meninos (58,3%), com idades entre 5-9 anos. O regime de quimioterapia mais comumente utilizado foi o protocolo BFM-95. A maioria das crianças (83,3%) teve um registro de alguma manifestação oral durante o tratamento. Ao exame intraoral, 17 crianças (50%) apresentaram ter pelo menos uma manifestação oral. A manifestação mais frequente foi mucosite (26,5%), seguido por sangramento gengival (23,4%). Baixa escolaridade materna foi associada com aumento da frequência de mucosite, assim como a quimioterapia de indução e quimioterapia de manutenção após recaída. Conclusões: Anormalidades orais são comuns em crianças que receberam quimioterapia e mucosite foi a manifestação mais prevalente. As lesões foram associados com a fase de indução da quimioterapia. Mucosite não foi associado com o estado de saúde oral.
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During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins.
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Dano ao DNA/genética , Reparo do DNA/genética , DNA Liase (Sítios Apurínicos ou Apirimidínicos)/genética , Escherichia coli/genética , Leishmania braziliensis/genética , Mutação/genética , Sequência de Aminoácidos , Escherichia coli/enzimologia , Proteínas de Escherichia coli/genética , Dados de Sequência MolecularRESUMO
During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins.