RESUMO

Aims: This study evaluated the effectiveness of different mechanical protocols using rotary brush (RB), ultrasonic tip, and oscillatory system (OS) associated with 2.5% sodium hypochlorite (NaOCl) in the removal of residues and dentin permeability in the cervical and apical segments of the post space. Settings and Design: Experimental in vitro study. Methods: Forty roots from human first molars were prepared and divided into four groups according to the cleaning protocols: Control (CO), NaOCl 2.5% conventional irrigation with a syringe; RB associated with NaOCl 2.5%; ultrasonic tip (US) associated with NaOCl 2.5%; OS associated with NaOCl 2.5%. Statistical Analysis Used: The persistence of residues was evaluated by scanning electron microscopy and data submitted to Kruskal-Wallis and Dunn tests, and dentin permeability evaluated by confocal microscopy and date submitted to ANOVA one-way and Tukey's tests (P = 0.05). Results: There were no differences in residue cleaning among the CO, RB, US, and OS groups, regardless of the analyzed root segment (P > 0.05). When comparing groups, dentin permeability in the CO group was significantly lower in the cervical segment (P < 0.05). Conclusions: The OS group promoted the highest permeability value in the apical segment. RB, US, and OS are similar in removing residues from the post space. However, OS results in higher dentin permeability in the apical segment.

RESUMO

This study compared the pH and calcium ion release of calcium silicate- (Bio-C Temp) and calcium hydroxide-based (Ultracal XS) medications. Intracanal remnants of both medications were also evaluated using SEM-EDS after the removal protocol. Thirty-five bovine teeth were prepared. Fifteen were filled with Bio-C Temp and 15 with Ultracal XS. Five remained without intracanal medication (control group). Five samples from each experimental time (i.e.. 24, 72, and 168 hours) were used to measure pH and calcium ions release using a digital pH meter and microplate reader, respectively. Afterward, the peaks of the chemical elements composing both medications were analyzed in SEM-EDS. One-way ANOVA and Tukey's post hoc test analyzed the pH and calcium ion release data. Student's t-test compared the medications in each experimental time. SEM-EDS described the percentage of chemical elements in the samples. Bio-C Temp and Ultracal XS showed a significant pH increase from 24 to 168 hours (p<0.05). Ultracal XS showed a higher pH value at 24 hours than Bio-C Temp (p<0.05) but were similar at 72 and 168h (p > 0.05). Calcium ion release did not depend on the experimental period (p > 0.05). Bio-C Temp showed lower calcium ions release than Ultracal XS at 24 hours (p<0.05). SEM-EDS analyses showed the remains of both medications, but the concentration of Si, Al, and W ions was present only in the calcium silicate-based medication. Bio-C Temp presented alkaline pH and a satisfactory calcium ion release over the time. The remaining of both medications were present after the protocols for paste removal.

Assuntos

Cálcio , Materiais Restauradores do Canal Radicular , Animais , Bovinos , Cavidade Pulpar , Hidróxido de Cálcio , Concentração de Íons de Hidrogênio , Bandagens , Análise Espectral , Materiais Restauradores do Canal Radicular/química

RESUMO

Abstract This study compared the pH and calcium ion release of calcium silicate- (Bio-C Temp) and calcium hydroxide-based (Ultracal XS) medications. Intracanal remnants of both medications were also evaluated using SEM-EDS after the removal protocol. Thirty-five bovine teeth were prepared. Fifteen were filled with Bio-C Temp and 15 with Ultracal XS. Five remained without intracanal medication (control group). Five samples from each experimental time (i.e.. 24, 72, and 168 hours) were used to measure pH and calcium ions release using a digital pH meter and microplate reader, respectively. Afterward, the peaks of the chemical elements composing both medications were analyzed in SEM-EDS. One-way ANOVA and Tukey's post hoc test analyzed the pH and calcium ion release data. Student's t-test compared the medications in each experimental time. SEM-EDS described the percentage of chemical elements in the samples. Bio-C Temp and Ultracal XS showed a significant pH increase from 24 to 168 hours (p<0.05). Ultracal XS showed a higher pH value at 24 hours than Bio-C Temp (p<0.05) but were similar at 72 and 168h (p > 0.05). Calcium ion release did not depend on the experimental period (p > 0.05). Bio-C Temp showed lower calcium ions release than Ultracal XS at 24 hours (p<0.05). SEM-EDS analyses showed the remains of both medications, but the concentration of Si, Al, and W ions was present only in the calcium silicate-based medication. Bio-C Temp presented alkaline pH and a satisfactory calcium ion release over the time. The remaining of both medications were present after the protocols for paste removal.

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Resumo Este estudo comparou o pH e a liberação de íons cálcio de medicações intracanais a à base de silicato de cálcio (Bio-C Temp) e à base de hidróxido de cálcio (Ultracal XS). Remanescentes de ambas as medicações também foram avaliados usando microscopia eletrônica de varredura e espectroscopia de dispersão de energia após o protocolo de remoção. Trinta e cinco dentes bovinos foram preparados. Quinze dentes foram preenchidos com Bio-C Temp e 15 com Ultracal XS. Cinco permaneceram sem medicação intracanal (grupo controle). Cinco amostras de cada tempo experimental (ou seja, 24, 72 e 168 horas) foram usadas para medir o pH e a liberação de íons de cálcio usando um medidor de pH digital e um leitor de microplacas, respectivamente. Em seguida, os picos dos elementos químicos que compõem os dois medicamentos foram analisados ​​em microscopia eletrônica de varredura e por espectroscopia de dispersão de energia. Os testes One-way ANOVA e post hoc de Tukey analisaram os dados de pH e liberação de íons cálcio. O teste t de Student comparou as medicações em cada tempo experimental. A microscopia eletrônica de varredura e a espectroscopia de dispersão de energia descreveu a porcentagem de elementos químicos nas amostras. O Bio-C Temp e o Ultracal XS mostraram um aumento significativo de pH de 24 a 168 horas (p<0,05). O Ultracal XS apresentou um valor de pH mais alto em 24 horas do que o Bio-C Temp (p<0,05), mas foi semelhante em 72 e 168h (p > 0,05). A liberação de íons cálcio não dependeu do período experimental (p> 0,05). O Bio-C Temp apresentou menor liberação de íons de cálcio do que Ultracal XS em 24 horas (p<0,05). As análises de microscopia eletrônica de varredura e espectroscopia de dispersão de energia mostraram remanescentes de ambas as medicações, mas a concentração de íons Si, Al e W estavam presentes apenas na medicação à base de silicato de cálcio. O Bio-C Temp apresentou pH alcalino e maior liberação de íons cálcio. Remanescentes de ambas as medicações estiveram presentes após os protocolos de remoção da pasta.

RESUMO

Recent formulations of resin-based composites have incorporated different combinations of materials. However, the mechanical and bonding behavior of these materials with intraradicular posts are unclear. This study aimed to evaluate the effect of light-cure and dual-cure resin composite posts on the fracture resistance of endodontically-treated teeth. Materials and Methods: Ninety extracted human upper canines were selected and randomly divided into nine groups (n=10): (G1) endodontically treated teeth without endodontic posts; (G2) glass-fiber post cemented with glass-ionomer cement; (G3) endodontic post by dual-cure composite resin (Rebilda DC); (G4) endodontic post by dual-cure composite resin (Cosmecore); (G5) endodontic post by dual-cure composite resin (Bis-Core); (G6) endodontic post by light-cure composite resin; (G7) glass-fiber post customized with flowable composite resin; (G8) glass-fiber post cemented with light-cure composite resin; (G9) glass-fiber post cemented with self-adhesive resin cement. After the post insertion, all specimens were subjected to mechanical (250,000 cycles) and thermocycling (6000 cycles, 5 °C/55 °C) and immediate loading at 45 degrees in a universal testing machine until fracture. The data were analyzed by one-way ANOVA and multiple comparisons using the Fisher LSD Method (p < 0 05). Results: The mean failure loads (±SD) for the groups ranged from 100.7 ± 22.6 N to 221.9 ± 48.9 N. The G1 group (without endodontic posts) had a higher fracture strength than all experimental groups (p < 0.001). Conclusions: Within the limitations, the light- and dual-cure post technique did not present lower fracture resistance values as compared to the conventional glass-fiber post.

RESUMO

Resin-based composites (RBCs) have transformed restorative dentistry and its procedures. However, the characteristics of RBCs have been modified over the years to enhance the physical and chemical properties of the materials. This context raises the need for studies that evaluate whether the properties of the RBCs that are commercially available are clinically adequate with different curing modes. This study aimed to evaluate the mechanical behavior of commercial RBCs after undergoing different curing modes. Twenty-three RBCs of different classes were evaluated. For curing the specimens, a microwave (BMS45, Brastemp) (for 3 min at 450 W) and three LED units were used: an Emitter A Fit (Schuster (second generation)) (light-curing for 15 s with an irradiance of 1250 mW/cm2), VALO (Ultradent (third generation)) (light-curing for 15 s with an irradiance of 1100 mW/cm2), and Emitter Now Duo (Schuster (second generation)) (light-curing for 15 s with an irradiance of 1100 mW/cm2). A total of 670 RBC specimens of 8 mm in diameter and 1 mm in depth were obtained. Afterward, a biaxial flexure strength test was performed until the failure of the specimens, using a universal testing machine set at a speed of 0.5 mm/min. The same specimens were subjected to infrared spectroscopy for evaluating the degree of conversion. Tukey's test was used for multiple comparisons at a significance level of 5%. The light-curing mode did not affect the flexure strength of the RBCs (p > 0.05), but the type and shade of RBCs did so (p < 0.05). In conclusion, the type of RBC directly interferes with the mechanical behavior of the material. However, the curing modes within the same RBC did not change the mechanical properties.

RESUMO

Neuropathic pain after brachial plexus injury (NPBPI) is a highly disabling clinical condition and is increasingly prevalent due to increased motorcycle accidents. Currently, no randomized controlled trials have evaluated the effectiveness of non-invasive brain stimulation techniques such as repetitive transcranial magnetic stimulation (rTMS) and transcranial direct-current stimulation (tDCS) in patients suffering from NPBPI. In this study, we directly compare the efficacy of 10-Hz rTMS and anodal 2 mA tDCS techniques applied over the motor cortex (5 daily consecutive sessions) in 20 patients with NPBPI, allocated into 2 parallel groups (active or sham). The order of the sessions was randomised for each of these treatment groups according to a crossover design and separated by a 30-day interval. Scores for "continuous" and "paroxysmal" pain (primary outcome) were tabulated after the last stimulation day and 30 days after. Secondary outcomes included the improvement in multidimensional aspects of pain, anxiety state and quality of life from a qualitative and quantitative approach. Active rTMS and tDCS were both superior to sham in reducing continuous (p < 0.001) and paroxysmal (p = 0.002; p = 0.02) pain as well as in multidimensional aspects of pain (p = 0.001; p = 0.002) and anxiety state (p = < 0.001; p = 0.005). Our results suggest rTMS and tDCS are able to treat NPBPI with little distinction in pain and anxiety state, which may promote the use of tDCS in brachial plexus injury pain management, as it constitutes an easier and more available technique.Clinical Trial Registration: http://www.ensaiosclinicos.gov.br/, RBR-5xnjbc - Sep 3, 2018.

Assuntos

Ansiedade/terapia , Plexo Braquial/lesões , Neuralgia/terapia , Estimulação Transcraniana por Corrente Contínua/métodos , Estimulação Magnética Transcraniana/métodos , Adulto , Ansiedade/etiologia , Ansiedade/psicologia , Estudos Cross-Over , Método Duplo-Cego , Feminino , Humanos , Masculino , Neuralgia/diagnóstico , Neuralgia/etiologia , Neuralgia/psicologia , Manejo da Dor/métodos , Medição da Dor/estatística & dados numéricos , Projetos Piloto , Placebos , Qualidade de Vida , Resultado do Tratamento , Adulto Jovem

RESUMO

The treatment of multibacillary cases of leprosy with multidrug therapy (MDT) comprises 12 doses of a combination of rifampicin, dapsone and clofazimine. Previous studies have described the immunological phenotypic pattern in skin lesions in multibacillary patients. Here, we evaluated the effect of MDT on skin cell phenotype and on the Mycobacterium leprae-specific immune response. An analysis of skin cell phenotype demonstrated a significant decrease in MRS1 (SR-A), CXCL10 (IP-10) and IFNG (IFN-γ) gene and protein expression after MDT release. Patients were randomized according to whether they experienced a reduction in bacillary load after MDT. A reduction in CXCL10 (IP-10) in sera was associated with the absence of a reduction in the bacillary load at release. Although IFN-γ production in response to M. leprae was not affected by MDT, CXCL10 (IP-10) levels in response to M. leprae increased in cells from patients who experienced a reduction in bacillary load after treatment. Together, our results suggest that CXCL10 (IP-10) may be a good marker for monitoring treatment efficacy in multibacillary patients.

Assuntos

Quimiocina CXCL10/sangue , Hansenostáticos/uso terapêutico , Hanseníase/tratamento farmacológico , Pele/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Carga Bacteriana/efeitos dos fármacos , Biomarcadores/sangue , Quimiocina CXCL10/imunologia , Quimioterapia Combinada , Feminino , Humanos , Hansenostáticos/administração & dosagem , Hanseníase/imunologia , Masculino , Pessoa de Meia-Idade , Mycobacterium leprae/imunologia , Pele/microbiologia , Pele/patologia , Resultado do Tratamento , Adulto Jovem

RESUMO

Transcranial Direct Current Stimulation (tDCS) has been used as an alternative treatment for pain reduction in fibromyalgia. In this study, in addition to behavioral measures, we analyzed oscillations in alpha 2 frequency band in the frontal, occipital, and parietal regions, in response to the application of two neuromodulation protocols in fibromyalgia. The study was a randomized, double-blind, placebo-controlled clinical trial with 31 women diagnosed with fibromyalgia. The participants were allocated to three groups with the anodic stimulation applied on the left motor cortex: Group 1, for five consecutive days; Group 2, for 10 consecutive days; and Group 3, sham stimulation for five consecutive days. Statistical analysis showed a reduction in pain intensity after treatment for groups in general [F (1.28) = 8.02; p = 0.008; η2 = 0.223], in addition to a reduction in alpha 2 in the frontal (p = 0.039; d = 0.384) and parietal (p = 0.021; d = 0.520) regions after the treatment on five consecutive days. We conclude that neuromodulation protocols produced similar effects on pain reduction, but differed with respect to the changes in the alpha 2 frequency band in the frontal and parietal regions.

Assuntos

Fibromialgia/terapia , Dor/prevenção & controle , Adulto , Estimulação Encefálica Profunda , Método Duplo-Cego , Estimulação Elétrica , Terapia por Estimulação Elétrica/métodos , Eletroencefalografia , Eletrofisiologia , Feminino , Humanos , Pessoa de Meia-Idade , Neurociências , Manejo da Dor/métodos

RESUMO

In HIV-infected individuals, a paradoxical clinical deterioration may occur in preexisting leprosy when highly active antiretroviral therapy (HAART)-associated reversal reaction (RR) develops. Leprosy-HIV co-infected patients during HAART may present a more severe form of the disease (RR/HIV), but the immune mechanisms related to the pathogenesis of leprosy-HIV co-infection remain unknown. Although the adaptive immune responses have been extensively studied in leprosy-HIV co-infected individuals, recent studies have described that innate immune cells may drive the overall immune responses to mycobacterial antigens. Monocytes are critical to the innate immune system and play an important role in several inflammatory conditions associated with chronic infections. In leprosy, different tissue macrophage phenotypes have been associated with the different clinical forms of the disease, but it is not clear how HIV infection modulates the phenotype of innate immune cells (monocytes or macrophages) during leprosy. In the present study, we investigated the phenotype of monocytes and macrophages in leprosy-HIV co-infected individuals, with or without RR. We did not observe differences between the monocyte profiles in the studied groups; however, analysis of gene expression within the skin lesion cells revealed that the RR/HIV group presents a higher expression of macrophage scavenger receptor 1 (MRS1), CD209 molecule (CD209), vascular endothelial growth factor (VEGF), arginase 2 (ARG2), and peroxisome proliferator-activated receptor gamma (PPARG) when compared with the RR group. Our data suggest that different phenotypes of tissue macrophages found in the skin from RR and RR/HIV patients could differentially contribute to the progression of leprosy.

Assuntos

Terapia Antirretroviral de Alta Atividade/efeitos adversos , Infecções por HIV/imunologia , HIV-1/fisiologia , Hanseníase/imunologia , Macrófagos/imunologia , Monócitos/imunologia , Mycobacterium leprae/fisiologia , Adulto , Idoso , Diferenciação Celular , Coinfecção , Progressão da Doença , Feminino , Infecções por HIV/complicações , Infecções por HIV/terapia , Humanos , Hanseníase/complicações , Hanseníase/terapia , Masculino , Pessoa de Meia-Idade , Receptores Depuradores Classe A/metabolismo

RESUMO

Objetivou-se com este trabalho avaliar o desempenho do método do tanque classe “A” (TCA) e Hargreaves-Samani (HS), comparados ao método de Penman-Monteith (PM), para determinação da evapotranspiração de referência (ET0) no município de Januária, MG. Foram utilizados os dados climáticos do período 01 de janeiro de 2014 a 31 de dezembro de 2016, pertencente ao banco de dados da estação meteorológica do INMET, localizada no município. A partir dos dados obtidos foram realizadas análises de correlação entre os métodos TCA e HS com o PM, de regressão linear e determinação dos índices de concordância de Willmott e de desempenho. De acordo com os resultados obtidos, pode-se concluir que o método do TCA superestima a ET0 na região de Januária-MG, mas teve desempenho bom quando ajustado ao método de PM. Quanto ao método de HS, os resultados obtidos mostram que este superestimou a ET0na maioria dos meses, porém apresentou coeficiente de correlação alto e desempenho satisfatório, sendo assim, ambos os métodos podem ser utilizados para estimar a ET0 na região de Januária MG, quando não se dispuser de todos os dados necessários para determinação por meio do método de PM.(AU)

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The objective of this work was to evaluate the performance of the class “A” (TCA) and Hargreaves-Samani (HS) method, compared to the Penman-Monteith (PM) method, to determine the reference evapotranspiration (ET0) in Januária, MG. We used the weather data from January 1, 2014 to December 31, 2016, belonging to the database of the INMET weather station, located in the municipality. From the obtained data, correlation analyzes between the TCA and HS methods with the PM, linear regression and determination of the Willmott agreement and performance indices were performed. According to the results obtained, it can be concluded that the ACT method overestimates the ET0 in the region of Januária-MG, but performed well when adjusted to the PM method. As for the HS method, the results show that it overestimated the ET0 in most months, but presented a high correlation coefficient and satisfactory performance, so both methods can be used to estimate the ET0 in the region of Januária MG, when not having all the data necessary for determination by the PM method.(AU)

Assuntos

Evapotranspiração , Tanques de Armazenamento , Consumo Doméstico de Água , Usos da Água , Desperdício de Água/prevenção & controle

RESUMO

Objetivou-se com este trabalho avaliar o desempenho do método do tanque classe “A” (TCA) e Hargreaves-Samani (HS), comparados ao método de Penman-Monteith (PM), para determinação da evapotranspiração de referência (ET0) no município de Januária, MG. Foram utilizados os dados climáticos do período 01 de janeiro de 2014 a 31 de dezembro de 2016, pertencente ao banco de dados da estação meteorológica do INMET, localizada no município. A partir dos dados obtidos foram realizadas análises de correlação entre os métodos TCA e HS com o PM, de regressão linear e determinação dos índices de concordância de Willmott e de desempenho. De acordo com os resultados obtidos, pode-se concluir que o método do TCA superestima a ET0 na região de Januária-MG, mas teve desempenho bom quando ajustado ao método de PM. Quanto ao método de HS, os resultados obtidos mostram que este superestimou a ET0na maioria dos meses, porém apresentou coeficiente de correlação alto e desempenho satisfatório, sendo assim, ambos os métodos podem ser utilizados para estimar a ET0 na região de Januária MG, quando não se dispuser de todos os dados necessários para determinação por meio do método de PM.

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The objective of this work was to evaluate the performance of the class “A” (TCA) and Hargreaves-Samani (HS) method, compared to the Penman-Monteith (PM) method, to determine the reference evapotranspiration (ET0) in Januária, MG. We used the weather data from January 1, 2014 to December 31, 2016, belonging to the database of the INMET weather station, located in the municipality. From the obtained data, correlation analyzes between the TCA and HS methods with the PM, linear regression and determination of the Willmott agreement and performance indices were performed. According to the results obtained, it can be concluded that the ACT method overestimates the ET0 in the region of Januária-MG, but performed well when adjusted to the PM method. As for the HS method, the results show that it overestimated the ET0 in most months, but presented a high correlation coefficient and satisfactory performance, so both methods can be used to estimate the ET0 in the region of Januária MG, when not having all the data necessary for determination by the PM method.

Assuntos

Consumo Doméstico de Água , Desperdício de Água/prevenção & controle , Evapotranspiração , Tanques de Armazenamento , Usos da Água

RESUMO

The micronucleus test has been applied for more than three decades in tadpoles, generating an early warning of environmental quality. In this study, we reviewed 48 articles on the micronucleus test in tadpoles, published between 1987 and 2018. The findings reveal that pesticides have been the main topic discussed in the induction of micronucleus and other nuclear abnormalities in anuran larvae to the detriment of the widespread use of compounds used in agriculture. In addition to pesticides, a number of other xenobiotic agents have been targeted for genotoxic damage, such as heavy metals, radiation and wastewater. An appeal is reported to environmental contaminants, which when released naturally into the environment or because of human activities may contaminate aquatic habitats, threatening populations of tadpoles that depend on these environments for their survival. Larvae can bioaccumulate these contaminants that cause progressive impacts, ranging from DNA damage to metamorphosis delays, as well as malformations. We found that Argentina is the main driving force for the application of this test in anuran larvae along with Brazil. Different erythrocyte malformations have been reported for the erythrocyte nuclear abnormalities test, binucleated cells, nuclear buds, notched, lobed, reniform, nuclear bebbled, anucleated, picnotic and apoptotic cells are the most cited. In summary, the presence of chemical or physical agents, along with other disturbances of the habitat, can have a significant impact on the life history of the species, contributing to the decline of anuran populations.

Assuntos

Anuros/genética , Ecotoxicologia/tendências , Eritrócitos/efeitos dos fármacos , Larva/efeitos dos fármacos , Larva/genética , Testes para Micronúcleos , Agricultura , Animais , Argentina , Brasil , Dano ao DNA , Ecossistema , Monitoramento Ambiental/métodos , Poluição Ambiental , Eritrócitos/patologia , Eritrócitos/fisiologia , Metais Pesados/toxicidade , Metamorfose Biológica/efeitos dos fármacos , Publicações Seriadas , Poluentes Químicos da Água/toxicidade

RESUMO

Erythraeidae Robineau-Desvoidy has 60 genera worldwide, of which Leptus Latreille has around 260 species described, with 15 of these recorded from Brazil. Here, we describe Leptus (Leptus) flechtmanni sp. n. from a cave in the state of Pará. Additional material attributed to this species is reported from other caves and also parasitizing a harvestman. In addition, SEM images and new Brazilian host records (Diplopoda and Hemiptera) are presented for Leptus (Leptus) haitlingeri from the states of São Paulo and Rio de Janeiro.

RESUMO

Objetivo: identificar as experiências culturais relacionadas às decisões maternas para a amamentação entre dois países, visando compreender seus significados. Método: pesquisa qualitativa através de estudo de caso, sendo realizadas nove entrevistas semiestruturadas, com mulheres brasileiras e francesas, no período de abril a dezembro de 2017. Aplicou-se a análise temática de Bardin aos depoimentos. O projeto da pesquisa teve anuência de Comitê de Ética em Pesquisa. Resultados: quatro categorias emergiram da análise dos depoimentos: escolha, cultura e influências; entre benefícios e prazer; idealismo da amamentação; relação com o corpo: dor, pudor e sexualidade. Conclusão: as entrevistadas brasileiras apontam para uma valorização da mulher que amamenta e uma culpabilização daquela que não o faz. E para as francesas a sociedade veicula a ideia de que amamentação significa falta de liberdade. Nos dois lados ainda falta a mulher se apropriar dos seus desejos.

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Objective: to identify the cultural experiences related to maternal breastfeeding decisions between two countries, aiming to understand their meanings. Methods: qualitative research through case study. Nine semi-structured interviews were conducted with Brazilian and French women, from April to December 2017. Bardin's thematic analysis was applied to the statements. The study was approved by ethics research committee. Results: four categories emerged: choice, culture and influences; between benefits and pleasure; breastfeeding idealism; relationship with the body: pain, shame and sexuality. Conclusion: in Brazil there is an appreciation of women who breastfeed and a blame for those who do not. In France society conveys the idea that breastfeeding means lack of freedom. In both countries the woman still needs to take ownership of her wishes.

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Objetivo: identificar las experiencias culturales relacionadas con las decisiones de lactancia materna entre dos países, con el objetivo de comprender sus significados. Método: investigación cualitativa a través del estudio de caso. Fueron nueve entrevistas semiestructuradas con mujeres brasileñas y francesas, de abril a diciembre de 2017. El análisis temático de Bardin se aplicó a los datos. La investigación tuvo anuencia del Comité de Ética en Investigación. Resultados: cuatro categorías emergieron: elección, cultura e influencias; entre beneficios y placer; idealismo de la lactancia materna; relación con el cuerpo: dolor, pudor y sexualidad. Conclusion: en Brasil, hay valorización de la mujer que amamanta y culpabilización de aquella que no lo hace. En Francia, la sociedad vehicula la idea de que la lactancia significa falta de libertad. En los dos países todavía falta la mujer apropiarse de sus deseos.

Assuntos

Humanos , Feminino , Aleitamento Materno , Aleitamento Materno/psicologia , Sexualidade , Cultura , Vergonha , Brasil , Lactação , Pesquisa Qualitativa

RESUMO

Despite advances in the treatment of Alzheimer's disease (AD), there is currently no prospect of a cure, and evidence shows that multifactorial interventions can benefit patients. A promising therapeutic alternative is the use of transcranial direct current stimulation (tDCS) simultaneously with cognitive intervention. The combination of these non-pharmacological techniques is apparently a safe and accessible approach. This study protocol aims to compare the efficacy of tDCS and cognitive intervention in a double-blind, randomized and factorial clinical trial. One hundred participants diagnosed with mild-stage AD will be randomized to receive both tDCS and cognitive intervention, tDCS, cognitive intervention, or placebo. The treatment will last 8 weeks, with a 12-month follow-up. The primary outcome will be the improvement of global cognitive functions, evaluated by the AD Assessment Scale, cognitive subscale (ADAS-Cog). The secondary outcomes will include measures of functional, affective, and behavioral components, as well as a neurophysiological marker (Brain-derived neurotrophic factor, BDNF). This study will enable us to assess, both in the short and long term, whether tDCS is more effective than the placebo and to examine the effects of combined therapy (tDCS and cognitive intervention) and isolated treatments (tDCS vs. cognitive intervention) on patients with AD. Clinical Trial Registration: www.ClinicalTrials.gov, identifier NCT02772185-May 5, 2016.

RESUMO

This study reviews global trends in the publication of papers on the micronucleus test of the exfoliated cells of the oral mucosa in mammals as an approach for environmental biomonitoring. This test has been widely used due to its precision for the detection of chromosome damage. Our temporal analysis showed a significant increase (p < 0.05) in the publication of papers on the oral mucosa over the past 33 years. Brazil was the country that published most papers (24% of the total), followed by India (16%), the USA (10%), Mexico (7%), and Turkey (6%). A further 30 countries contributed the other 37% of the papers. Overall, 99% of the micronucleus studies involved human subjects, and only 1% involved other mammals. As many wild mammals are subject to the same environmental pressures as humans, in particular chemical pollution, it seems likely that many species are equally susceptible to genotoxicogical damage. We emphasize the importance of applying this technique to the analysis of the oral mucosa of wild mammals, as well as the evaluation of its effectiveness, as observed in humans, and the expansion of the available approaches to the monitoring of environmental quality.

Assuntos

Monitoramento Ambiental , Testes para Micronúcleos , Mucosa Bucal/efeitos dos fármacos , Mutagênicos/análise , Animais , Núcleo Celular , Dano ao DNA , Monitoramento Ambiental/métodos , Humanos , Micronúcleos com Defeito Cromossômico , Testes para Micronúcleos/métodos

RESUMO

Purpose the aim of this study was to evaluate the pattern of human papillomavirus (HPV) detection in an 11.3-year post-vaccination period in a cohort of adolescent and young women vaccinated or not against HPV 16/18. Methods a subset of 91 women from a single center participating in a randomized clinical trial (2001-2010, NCT00689741/00120848/00518336) with HPV 16/18 AS04-adjuvanted vaccine was evaluated. All women received three doses of the HPV vaccine (n = 48) or a placebo (n = 43), and cervical samples were collected at 6-month intervals. Only in this center, one additional evaluation was performed in 2012. Up to 1,492 cervical samples were tested for HPV-DNA and genotyped with polymerase chain reaction (PCR). The vaccine group characteristics were compared by Chi-square or Fisher exact or Mann-Whitney test. The high-risk (HR)-HPV 6-month-persistent infection rate was calculated. The cumulative infection by HPV group was evaluated by the Kaplan-Meier method and the log-rank test. Results the cumulative infection with any type of HPV in an 11.3-year period was 67% in the HPV vaccine group and 72% in the placebo group (p = 0.408). The longitudinal analysis showed an increase of 4% per year at risk for detection of HR-HPV (non-HPV 16/18) over time (p = 0.015), unrelated to vaccination. The cumulative infection with HPV 16/18 was 4% for the HPV vaccine group and 29% for the placebo group (p = 0.003). There were 43 episodes of HR-HPV 6-month persistent infection, unrelated to vaccination. Conclusions this study showed the maintenance of viral detection rate accumulating HR-HPV (non-HPV-16-18) positive tests during a long period post-vaccination, regardless of prior vaccination. This signalizes that the high number of HPV-positive tests may be maintained after vaccination.

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Objetivos avaliar o padrão de detecção do papilomavírus humano (HPV) em um período de 11.3 anos após a vacinação em uma coorte de adolescentes e mulheres jovens vacinadas ou não contra HPV 16/18. Métodos avaliou-se um subgrupo de 91 mulheres de um único centro, participantes de ensaio clínico randomizado (2001­2010, NCT00689741/00120848/00518336) com a vacina contra HPV 16/18 com adjuvante AS04. Todas as mulheres receberam três doses de vacina contra HPV (n = 48) ou placebo (n = 43), e tiveram amostras cervicais coletadas em intervalos de 6 meses. Somente neste centro, uma avaliação adicional foi realizada em 2012. Um total de 1.492 amostras cervicais foram testadas para DNA-HPV e genotipadas com reação em cadeia da polimerase (RCP). As características dos grupos de vacina contra HPV ou placebo foram comparadas pelo teste de Qui-quadrado ou teste exato de Fisher ou teste de Mann-Whitney. A infecção persistente por 6 meses pelo HPV de alto risco (AR) foi calculada. A infecção cumulativa por grupo foi avaliada pelo método de Kaplan-Meier e pelo teste log-rank. Resultados a infecção cumulativa com qualquer tipo de HPV em 11.3 anos foi de 67% no grupo vacina contra HPV e de 72% no grupo placebo (p = 0,408). A análise longitudinal mostrou um aumento de 4% ao ano no risco de detecção de HR-HPV (não-HPV 16/18) ao longo do tempo (p = 0,015), não relacionado com a vacinação. A infecção cumulativa com HPV 16/18 foi de 4% para o grupo vacina contra HPV e 29% para o grupo placebo (p = 0,003). Houve 43 episódios de infecção persistente por 6 meses por HR-HPV, não relacionados com a vacinação. Conclusões este estudo mostrou a manutenção da taxa de detecção viral, acumulando testes positivos de HR-HPV (não HPV-16­18) durante longo período pós-vacinação, independentemente da vacinação prévia. Isto sinaliza que a alta positividade dos testes de HPV pode ser mantida após a vacinação.

Assuntos

Colo do Útero/virologia , Papillomaviridae/isolamento & purificação , Vacinas contra Papillomavirus , Feminino , Seguimentos , Papillomavirus Humano 16/imunologia , Papillomavirus Humano 18/imunologia , Humanos , Infecções por Papillomavirus/prevenção & controle , Estudos Prospectivos , Medição de Risco , Fatores de Tempo

RESUMO

Abstract Purpose the aim of this study was to evaluate the pattern of human papillomavirus (HPV) detection in an 11.3-year post-vaccination period in a cohort of adolescent and young women vaccinated or not against HPV 16/18. Methods a subset of 91 women from a single center participating in a randomized clinical trial (2001-2010, NCT00689741/00120848/00518336) with HPV 16/18 AS04- adjuvanted vaccine was evaluated. All women received three doses of the HPV vaccine (n = 48) or a placebo (n = 43), and cervical samples were collected at 6-month intervals. Only in this center, one additional evaluation was performed in 2012. Up to 1,492 cervical samples were tested for HPV-DNA and genotyped with polymerase chain reaction (PCR). The vaccine group characteristics were compared by Chi-square or Fisher exact or Mann-Whitney test. The high-risk (HR)-HPV 6-month-persistent infection rate was calculated. The cumulative infection by HPV group was evaluated by the Kaplan-Meier method and the log-rank test. Results the cumulative infection with any type of HPV in an 11.3-year period was 67% in the HPV vaccine group and 72% in the placebo group (p = 0.408). The longitudinal analysis showed an increase of 4% per year at risk for detection of HR-HPV (non-HPV 16/ 18) over time (p = 0.015), unrelated to vaccination. The cumulative infection with HPV 16/18 was 4% for the HPV vaccine group and 29% for the placebo group (p = 0.003). There were 43 episodes of HR-HPV 6-month persistent infection, unrelated to vaccination. Conclusions this study showed themaintenance of viral detection rate accumulating HR-HPV (non-HPV-16-18) positive tests during a long period post-vaccination, regardless of prior vaccination. This signalizes that the high number of HPV-positive testsmay be maintained after vaccination.

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Resumo Objetivos avaliar o padrão de detecção do papilomavírus humano (HPV) em um período de 11.3 anos após a vacinação em uma coorte de adolescentes e mulheres jovens vacinadas ou não contra HPV 16/18. Métodos avaliou-se um subgrupo de 91 mulheres de um único centro, participantes de ensaio clínico randomizado (2001-2010, NCT00689741/00120848/00518336) com a vacina contra HPV 16/18 com adjuvante AS04. Todas as mulheres receberam três doses de vacina contra HPV (n = 48) ou placebo (n = 43), e tiveram amostras cervicais coletadas em intervalos de 6 meses. Somente neste centro, uma avaliação adicional foi realizada em 2012. Um total de 1.492 amostras cervicais foram testadas para DNA-HPV e genotipadas com reação em cadeia da polimerase (RCP). As características dos grupos de vacina contra HPV ou placebo foram comparadas pelo teste de Qui-quadrado ou teste exato de Fisher ou teste de Mann-Whitney. A infecção persistente por 6meses pelo HPV de alto risco (AR) foi calculada. A infecção cumulativa por grupo foi avaliada pelo método de Kaplan-Meier e pelo teste log-rank. Resultados a infecção cumulativa com qualquer tipo de HPV em11.3 anos foi de 67% no grupo vacina contra HPV e de 72% no grupo placebo (p = 0,408). A análise longitudinal mostrou um aumento de 4% ao ano no risco de detecção de HR-HPV (não-HPV 16/18) ao longo do tempo (p = 0,015), não relacionado com a vacinação. A infecção cumulativa com HPV 16/18 foi de 4% para o grupo vacina contra HPV e 29% para o grupo placebo (p = 0,003). Houve 43 episódios de infecção persistente por 6 meses por HR-HPV, não relacionados com a vacinação. Conclusões este estudo mostrou a manutenção da taxa de detecção viral, acumulando testes positivos de HR-HPV (não HPV-16-18) durante longo período pósvacinação, independentemente da vacinação prévia. Isto sinaliza que a alta positividade dos testes de HPV pode ser mantida após a vacinação.

Assuntos

Humanos , Feminino , Papillomaviridae/isolamento & purificação , Colo do Útero/virologia , Vacinas contra Papillomavirus , Fatores de Tempo , Estudos Prospectivos , Seguimentos , Medição de Risco , Infecções por Papillomavirus/prevenção & controle , Papillomavirus Humano 16/imunologia , Papillomavirus Humano 18/imunologia

RESUMO

Objective. We compared the effects of transcranial direct current stimulation at different cortical sites (premotor and motor primary cortex) combined with constraint-induced movement therapy for treatment of stroke patients. Design. Sixty patients were randomly distributed into 3 groups: Group A, anodal stimulation on premotor cortex and constraint-induced movement therapy; Group B, anodal stimulation on primary motor cortex and constraint-induced movement therapy; Group C, sham stimulation and constraint-induced movement therapy. Evaluations involved analysis of functional independence, motor recovery, spasticity, gross motor function, and muscle strength. Results. A significant improvement in primary outcome (functional independence) after treatment in the premotor group followed by primary motor group and sham group was observed. The same pattern of improvement was highlighted among all secondary outcome measures regarding the superior performance of the premotor group over primary motor and sham groups. Conclusions. Premotor cortex can contribute to motor function in patients with severe functional disabilities in early stages of stroke. This study was registered in ClinicalTrials.gov database (NCT 02628561).

RESUMO

Este texto tem por objetivo propor a utilização do objeto intermediário e do "como se" como mediadores reflexivos necessários em uma intervenção psicossocial dirigida a autores de violência sexual contra crianças e adolescentes. Trata-se de pesquisa documental realizada em uma instituição de saúde. Os participantes foram 14 adultos ofensores sexuais, que integraram uma intervenção psicossocial grupal com oito sessões, que estão aqui descritas. A introdução do "como se" diminui a tensão na discussão dos temas e possibilita oportunidades de maior expressão na ação. A adoção do objeto intermediário constrói um ambiente lúdico que auxilia na interação entre os participantes. A orientação teórica e prática do Psicodrama permite o desvio da atenção para temas que têm grande comprometimento social e moral.

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This text aims to propose the use of an intermediate object and the "as if" technique as reflexive mediators needed in a psychosocial intervention addresses to perpetrators of sex violence against children and adolescents. It is a documentary research carried out in a health institution. Fourteen adult sex offenders participated of this study and joined a psychossocial intervention in group, developed in eight sessions, that are described herein. The introduction of the "as if" decreases the tension during the discussion of the themes and also provides opportunities for greater expression during action. The adoption of the intermediate object creates a recreational context that assists the interaction among the participants. The theoretical and practical orientation of psychodrama allows focused on themes that have great social and moral commitment

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Este texto tiene por objetivo proponer la utilización del objeto intermediario y del "como si", como mediadores necesarios en una intervención psicosocial centrada en autores de violencia sexual contra los niños y adolescentes. Se trata de una investigación documental realizada en una instituición de salud. Los participantes fueron 14 ofensores sexuales adultos, que formaron parte de una intervención psicosocial grupal desarrollada en ocho se siones, descritas en el presente trabajo. La aplicación del "como si" disminuye la tensión en la discusión de los temas, y posibilita oportunidades de mayor expresión en la acción. La adopción del objeto intermediario contribuye a crear un entorno lúdico que auxilia en el proceso interactivo entre los participantes. La orientación teórica y práctica del psicodrama permite centrar la atención en temas con gran compromiso social y moral.