RESUMO
Invertebrates can provide a valuable alternative to traditional vertebrate animal models for studying bacterial and fungal infections. This study aimed to establish the larvae of the coleoptera Tenebrio molitor (mealworm) as an in vivo model for evaluating virulence and horizontal gene transfer between Staphylococcus spp. After identifying the best conditions for rearing T. molitor, larvae were infected with different Staphylococcus species, resulting in dose-dependent killing curves. All species tested killed the insects at higher doses, with S. nepalensis and S. aureus being the most and least virulent, respectively. However, only S. nepalensis was able to kill more than 50% of larvae 72 h post-infection at a low amount of 105 CFU. Staphylococcus infection also stimulated an increase in the concentration of hemocytes present in the hemolymph, which was proportional to the virulence. To investigate T. molitor's suitability as an in vivo model for plasmid transfer studies, we used S. aureus strains as donor and recipient of a plasmid containing the gentamicin resistance gene aac(6')-aph(2â³). By inoculating larvae with non-lethal doses of each, we observed conjugation, and obtained transconjugant colonies with a frequency of 1.6 × 10-5 per donor cell. This study demonstrates the potential of T. molitor larvae as a reliable and cost-effective model for analyzing the virulence of Staphylococcus and, for the first time, an optimal environment for the plasmid transfer between S. aureus carrying antimicrobial resistance genes.
Assuntos
Tenebrio , Animais , Virulência/genética , Tenebrio/microbiologia , Staphylococcus/genética , Staphylococcus aureus/genética , Transferência Genética Horizontal , Larva/microbiologiaRESUMO
Aim: The present in vitro study aimed to evaluate the bond strength of a bulk fill composite on dentin surfaces prepared with the Er: YAG laser. Methods: Twenty-four permanent third molars were selected and divided into 2 groups: CP - Conventional preparation with high-speed handpiece (control) and LA (laser) - Preparation with Er: YAG laser. The occlusal surface was removed to expose coronal dentin, which was subsequently prepared with a high-speed handpiece or Er: YAG laser (350mJ, 4Hz, 1.5 ml/min water flow). Both groups were restored with Filtek One Bulk Fill (3M ESPE) composite resin. After 24 hours, the samples were evaluated for microtensile bond strength (µTBS), fracture pattern, and scanning electron microscopy (SEM). Results: The data obtained in the µTBS test were submitted to t-test (α=0.05). The results showed no difference in µTBS when the different types of cavity preparation were compared (ρ=0.091). Fracture patterns revealed the prevalence of cohesive fracture in composite resin in CP (83.3%) and adhesive fracture in LA (92.1%). In the SEM analysis, the LA group demonstrated the presence of gaps between the composite resin and the irradiated dentin surface. The hybrid layer exhibited more regularity with the presence of longer and uniform resin tags in the CP group. Conclusion: The type of cavity preparation did not influence the values of bulk fill composite resin µTBS to dentin. Fracture patterns and scanning electron microscopy analyses suggested less interference at the adhesive interface in preparations performed using CP
Assuntos
Adesividade , Resinas Compostas , Materiais Dentários , Lasers de Estado SólidoRESUMO
Introdução: Crianças menores de cinco anos são as principais vítimas de acidentes por ingestão de cáusticos, assim considerando a epidemiologia brasileira e dos riscos à exposição desses agentes ressalta-se a importância deste estudo que objetiva realizar uma análise descritiva dos casos de acidentes cáusticos em pacientes pediátricos atendidos em um hospital universitário de Minas Gerais. Método: Este é um estudo transversal descritivo, de caráter quantitativo, a partir dos dados obtidos de prontuários de pacientes pediátricos (0-13 anos) atendidos por ingestão de substâncias cáusticas, no Hospital de Clínicas da Universidade Federal de Uberlândia (Minas Gerais, Brasil). Resultados: Foram levantados 132 prontuários de crianças atendidas por ingestão de substâncias cáusticas de janeiro de 2011 a abril de 2018. Os acidentes acometeram crianças na faixa etária de 08 meses a 12 anos, sendo que 82,60% dos casos ocorreram em ambiente domiciliar. Entre as principais substâncias ingeridas estão os produtos de limpeza, quanto à sua composição química predominaram soda cáustica, hipoclorito de sódio e amoníaco. A endoscopia digestiva alta (EDA) foi realizada em 104 pacientes. Quase 13% das crianças apresentaram estenose esofágica e necessitaram de dilatação esofágica. No período estudado, foram realizados 296 procedimentos de dilatações, com média de 17,4 procedimentos por paciente. Discussão: Considerando que os acidentes cáusticos são prevalentes em crianças menores de cinco anos e em ambiente domiciliar, as principais substâncias ingeridas são aquelas de caráter alcalino, que causam lesão no trato respiratório e gastrointestinal, sendo a principal consequência a estenose esofágica. Ademais, não há protocolos bem definidos para o manejo e a condução de pacientes que fizeram a ingestão dessas substâncias. As principais limitações do estudo foram o preenchimento incompleto dos prontuários analisados e os trâmites burocráticos para o acesso aos mesmos. Conclusão: Predominaram os acidentes cáusticos domiciliares e em crianças menores de 2 anos, o que implica a necessidade de ações educativas e preventivas. [au]
Introduction: Children under five years old are the principal victims of accidents caused by the ingestion of caustics, so considering Brazilian epidemiology and the risks of exposure to these agents the importance of this study is emphasized. This study aims to carry out a descriptive analysis of cases of caustic accidents in pediatric patients treated in a university hospital in Minas Gerais. Method: This is a descriptive cross-sectional study, with a quantitative character, based on data obtained from medical records of pediatric patients (0-13 years old) treated for ingestion of caustic agents at the Hospital of the Federal University of Uberlândia (Minas Gerais, Brazil). Results: We collected 132 medical records of children treated for ingestion of caustic substances from January 2011 to April 2018. The accidents affected children aged between 8 months and 12 years, with 82.60% of the cases occurring in the home environment. Among the main substances ingested are cleaning products, as for their chemical composition, caustic soda, sodium hypochlorite, and ammonia predominated. Upper digestive endoscopy (UGE) was performed in 104 patients. Almost 13% of the children had esophageal strictures and required esophageal dilation. During the study period, 296 dilatation procedures were performed, with an average of 17.4 procedures per patient. Discussion: Considering that caustic accidents are prevalent in children under five years of age and the home environment, the main substances ingested are those of an alkaline nature, which cause injury to the respiratory and gastrointestinal tract, the main consequence being esophageal stenosis. Furthermore, there are no well-defined protocols for the management of patients who have ingested these substances. The main limitations of the study were the incomplete filling of the medical records analyzed and the bureaucratic procedures for accessing them. Conclusion: Caustic accidents predominated in children under two years old in the home environment, which implies the need for educational and preventive actions. [au]
RESUMO
As preconized by the One Health concept, the intimate relationship between pets and owners is a common source for the trade of microorganisms with zoonotic potential, and with them, antimicrobial resistance genes. In this work, we evaluated the presence of antimicrobial resistance genes, that are usually within mobile genetic elements, in a laboratory collection of 79 canine Staphylococcus strains, mostly Staphylococcus pseudintermedius and Staphylococcus coagulans. Resistance to tetracycline was observed in 34% of the strains, followed by resistance to erythromycin (21%) and gentamicin (19%). These phenotypes were partially correlated with the presence of the tetracycline resistance genes tet(M) and tet(K) in 64% and 44% of all strains, respectively; erythromycin resistance genes erm(A) and erm(C) in 53% and 23%; and gentamicin resistance gene aac(6')-aph(2â³) in 26% of the strains. At least 45% of the strains harbored high- and/or low-molecular weight plasmids, whose transfer may be facilitated by their widespread biofilm-forming capacity, and absence of restrictive CRISPR systems. We selected eight plasmid-bearing and multidrug resistant strains, which were submitted to plasmid curing by stress with SDS. No strain lost resistance during stressing cultivation but, by conjugation experiments, the S. pseudintermedius strain 27 transferred its plasmid-borne resistance to gentamicin, conferred by the aac(6')-aph(2â³) gene, to Staphylococcus aureus. The frequent empirical use of gentamicin to treat skin and ear infections in domestic dogs is likely to select resistant strains. Also, as demonstrated by our study, these strains can serve as gene reservoirs for human pathogens, such as S. aureus.
Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla , Gentamicinas/farmacologia , Plasmídeos/efeitos da radiação , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus/efeitos dos fármacos , Animais , CãesRESUMO
Nek4 is a serine/threonine kinase which has been implicated in primary cilia stabilization, DNA damage response, autophagy and epithelial-to-mesenchymal transition. The role of Nek4 in cancer cell survival and chemotherapy resistance has also been shown. However, the precise mechanisms by which Nek4 operates remain to be elucidated. Here, we show that Nek4 overexpression activates mitochondrial respiration coupled to ATP production, which is paralleled by increased mitochondrial membrane potential, and resistance to mitochondrial DNA damage. Congruently, Nek4 depletion reduced mitochondrial respiration and mtDNA integrity. Nek4 deficiency caused mitochondrial elongation, probably via reduced activity of the fission protein DRP1. In Nek4 overexpressing cells, the increase in mitochondrial fission was concomitant to enhanced phosphorylation of DRP1 and Erk1/2 proteins, and the effects on mitochondrial respiration were abolished in the presence of a DRP1 inhibitor. This study shows Nek4 as a novel regulator of mitochondrial function that may explain the joint appearance of high mitochondrial respiration and mitochondrial fragmentation.
Assuntos
Dinaminas , Dinâmica Mitocondrial , DNA Mitocondrial/metabolismo , Dinaminas/genética , Dinaminas/metabolismo , Mitocôndrias/metabolismo , Proteínas Mitocondriais/genética , Fosforilação , RespiraçãoRESUMO
Food allergy prevalence is increasing worldwide, therefore there is a high demand for reliable tests to correctly diagnose this disease. Knowledge of proteins allergenicity and how they react both in the body and in diagnostic tests is necessary to adequately assess the potential immunogenicity of both natural foods and those produced through biotechnological processes. Thus, our aim was to analyze the factors that influence the protein extraction of foods in terms of, immunogenicity and immunoassays sensitivity. Peanut proteins were extracted using four distinct extraction buffers with different pH values (physiological saline, tris buffer, borate buffer with and without ß-mercaptoethanol), the protein concentration was determined by the Lowry method and polyacrylamide electrophoresis (SDS-PAGE) was used to compare the protein profile of each extract. The immunogenicity of each extract was verified by sensitizing two mouse strains (Balb/c and C57Bl/6) with a solution containing 100 µg of the extracted proteins and was determined by ELISA. Results show that extraction with the distinct buffers resulted in protein solutions with different yields and profiles. The immunogenicity of the different extracts also demonstrated distinct patterns that varied depending on the extraction methods, mouse strain and in vitro test. Immunoreactivity varied in accordance with the protein extract used to coat the microtitration plates. In conclusion, the protein profile in the extracts is critically influenced by the salt composition and pH of the extraction buffers, this in turn influences both in vivo immunogenicity and in vitro immunoreactivity.
Assuntos
Alérgenos/imunologia , Alérgenos/isolamento & purificação , Arachis/imunologia , Ensaio de Imunoadsorção Enzimática , Imunoglobulina G/sangue , Hipersensibilidade a Amendoim/diagnóstico , Proteínas de Plantas/imunologia , Proteínas de Plantas/isolamento & purificação , Alérgenos/administração & dosagem , Animais , Biomarcadores/sangue , Soluções Tampão , Fracionamento Químico , Eletroforese em Gel de Poliacrilamida , Feminino , Humanos , Concentração de Íons de Hidrogênio , Injeções Subcutâneas , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Hipersensibilidade a Amendoim/imunologia , Proteínas de Plantas/administração & dosagem , Valor Preditivo dos Testes , Reprodutibilidade dos TestesRESUMO
NIMA-related kinases, or NEKs, are a family of Ser/Thr protein kinases involved in cell cycle and mitosis, centrosome disjunction, primary cilia functions, and DNA damage responses among other biological functional contexts in vertebrate cells. In human cells, there are 11 members, termed NEK1 to 11, and the research has mainly focused on exploring the more predominant roles of NEKs in mitosis regulation and cell cycle. A possible important role of NEKs in DNA damage response (DDR) first emerged for NEK1, but recent studies for most NEKs showed participation in DDR. A detailed analysis of the protein interactions, phosphorylation events, and studies of functional aspects of NEKs from the literature led us to propose a more general role of NEKs in DDR. In this review, we express that NEK1 is an activator of ataxia telangiectasia and Rad3-related (ATR), and its activation results in cell cycle arrest, guaranteeing DNA repair while activating specific repair pathways such as homology repair (HR) and DNA double-strand break (DSB) repair. For NEK2, 6, 8, 9, and 11, we found a role downstream of ATR and ataxia telangiectasia mutated (ATM) that results in cell cycle arrest, but details of possible activated repair pathways are still being investigated. NEK4 shows a connection to the regulation of the nonhomologous end-joining (NHEJ) repair of DNA DSBs, through recruitment of DNA-PK to DNA damage foci. NEK5 interacts with topoisomerase IIß, and its knockdown results in the accumulation of damaged DNA. NEK7 has a regulatory role in the detection of oxidative damage to telomeric DNA. Finally, NEK10 has recently been shown to phosphorylate p53 at Y327, promoting cell cycle arrest after exposure to DNA damaging agents. In summary, this review highlights important discoveries of the ever-growing involvement of NEK kinases in the DDR pathways. A better understanding of these roles may open new diagnostic possibilities or pharmaceutical interventions regarding the chemo-sensitizing inhibition of NEKs in various forms of cancer and other diseases.
Assuntos
Dano ao DNA/genética , Reparo do DNA/genética , HumanosRESUMO
Little is known about Nima-related kinase (NEKs), a widely conserved family of kinases that have key roles in cell-cycle progression. Nevertheless, it is now clear that multiple NEK family members act in networks, not only to regulate specific events of mitosis, but also to regulate metabolic events independently of the cell cycle. NEK5 was shown to act in centrosome disjunction, caspase-3 regulation, myogenesis, and mitochondrial respiration. Here, we demonstrate that NEK5 interacts with LonP1, an AAA+ mitochondrial protease implicated in protein quality control and mtDNA remodeling, within the mitochondria and it might be involved in the LonP1-TFAM signaling module. Moreover, we demonstrate that NEK5 kinase activity is required for maintaining mitochondrial mass and functionality and mtDNA integrity after oxidative damage. Taken together, these results show a new role of NEK5 in the regulation of mitochondrial homeostasis and mtDNA maintenance, possibly due to its interaction with key mitochondrial proteins, such as LonP1.
Assuntos
Proteases Dependentes de ATP/metabolismo , DNA Mitocondrial/genética , Mitocôndrias/metabolismo , Proteínas Mitocondriais/metabolismo , Quinases Relacionadas a NIMA/metabolismo , Linhagem Celular , Variações do Número de Cópias de DNA , Regulação da Expressão Gênica , Células HEK293 , Humanos , Mitocôndrias/genética , Quinases Relacionadas a NIMA/genética , Estresse Oxidativo , Mapas de Interação de ProteínasRESUMO
Staphylococcus nepalensis is a commensal bacterium from the oral microbiota of domestic cats, with a still obscure clinical importance. In this work, we analysed the ability of feline strains of S. nepalensis to transfer antimicrobial resistance genes to Staphylococcus aureus isolated from humans through plasmids. To this end, we first analysed all publicly available genomes from cat staphylococci using computational methods to build a pan-resistome. Genes that encode resistance to erythromycin, gentamicin, mupirocin and tetracycline, common to human and cat staphylococci and previously described to be located in mobile genetic elements, were chosen for the next analyses. We studied 15 strains of S. nepalensis, which were shown to be genetically different by GTG5-PCR. As observed by disc diffusion, resistance to tetracycline was widespread (80â%), followed by resistance to erythromycin (40â%), gentamicin (27â%) and mupirocin (7â%). The strains were positive for several antimicrobial resistance genes and more than half of them harboured plasmids. The loss of plasmids and resistance genes in some strains were induced by stress with SDS. Through conjugation experiments, we observed that these plasmids can be transferred to S. aureus, thus increasing its potential to resist drug therapy. Our findings show that S. nepalensis, an underestimated inhabitant of the cat microbiota, can be a reservoir of antimicrobial resistance genes for S. aureus and, like many other staphylococci, be an overlooked and silent threat to their animal hosts and humans living with them.
Assuntos
Reservatórios de Doenças/veterinária , Farmacorresistência Bacteriana/genética , Transferência Genética Horizontal , Staphylococcus/fisiologia , Animais , Animais Domésticos , Antibacterianos/farmacologia , Gatos , Reservatórios de Doenças/microbiologia , Farmacorresistência Bacteriana/efeitos dos fármacos , Genes Bacterianos , Variação Genética , Testes de Sensibilidade Microbiana , Plasmídeos/efeitos dos fármacos , Plasmídeos/genética , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/veterinária , Staphylococcus/efeitos dos fármacos , Staphylococcus/genética , Staphylococcus/isolamento & purificação , Staphylococcus aureus/genéticaRESUMO
Aim: The present study aimed to assess in vitro the effect ofviolet LED in tooth bleaching techniques associated or not withlow-concentration hydrogen peroxide gel on enamel surfaceroughness. Methods: Fifty-two enamel fragments of bovineteeth were flattened and polished (4x4x3 mm) and dividedinto four groups according to bleaching treatment: VL- VioletLED; HP- 7.5% hydrogen peroxide; HP+VL- 7.5% hydrogenperoxide + violet LED; C- No bleaching (control). Before thetreatments, all specimens were immersed in 20 mL of blacktea for six days, changing solutions every 24 h to simulatethe staining of specimens. Forty fragments were used toanalyze surface roughness (n=10) and 12 fragments wereused for the morphological analysis (SEM) (n=3). Results:The data were submitted to one-way ANOVA and a post-hocTukey test. The lower roughness values was observed for thegroup that did not receive bleaching treatment (C), differingsignificantly only from the group bleached with 7.5% hydrogenperoxide + violet LED (HP+VL) (p=0.0077). The remaininggroups did not show significant differences in roughnessvalues (p>0.05). The scanning electron microscopy analysisshowed irregularities on the enamel surface regardless ofthe treatment received. Conclusion: The results showedthat bleaching treatments with violet LED associated withlow-concentration hydrogen peroxide gels (7.5%) increasethe surface roughness of tooth enamel
Assuntos
Clareamento Dental , Esmalte Dentário , Peróxido de HidrogênioRESUMO
Introdução: o tratamento endodôntico em dentes com canais calcificados é extremamente desafiador. Durante o procedimento de localização do canal residual, uma quantidade excessiva de dentina pode ser removida, o que pode implicar em maior risco de fratura do elemento dentário. Somado a isso, a calcificação pulpar é o motivo mais comum de perfuração radicular durante o tratamento endodôntico. Métodos: tendo em vista a importância do desenvolvimento de técnicas seguras e precisas para o tratamento endodôntico de canais parcial ou totalmente calcificados, serão apresentados dois relatos de casos clínicos conduzidos segundo uma estratégia terapêutica recentemente descrita na literatura endodôntica. Resultados: o Endoguide tornou mais segura e eficaz a realização do tratamento endodôntico nos dois casos relatados. Conclusão: o guia endodôntico auxiliou na localização do canal radicular calcificado e ajudou a evitar iatrogenias durante sua localização (AU).
Introduction: Endodontic treatment in teeth with calcified root canals is extremely challenging. During the procedure of locating the root canal, an excessive amount of residual dentin can be removed, which may lead to a greater risk of tooth fracture. In addition, pulp calcification is the most common reason for root perforation during endodontic treatment. Methods: Considering the importance of developing safe and efficient techniques for endodontic treatment of partially or completely calcified root canals, these two clinical cases were conducted in accordance with the therapeutic strategy recently described in the endodontic literature. Results: Endoguide made endodontic treatment safer and more effective in the two reported cases Conclusion: The endodontic guide used in the present cases have helped to locate the calcified root canal and to prevent iatrogenies during location (AU).
Assuntos
Humanos , Masculino , Feminino , Adulto , Calcificação de Dente , Calcificações da Polpa Dentária , Tomografia Computadorizada de Feixe Cônico , Diagnóstico por Imagem , Cavidade PulparRESUMO
The close contact between pets and their owners is a potential source for microorganisms and genetic material exchange. Staphylococcus species considered as harmless inhabitants of animals' and humans' microbiota can act as reservoirs of antimicrobial resistance genes to more virulent species, thereby increasing their potential to resist drug therapy. This process could be inhibited by the antiplasmid immunity conferred by CRISPR systems. On the other hand, CRISPR spacer sequences can be explored as molecular clocks to track the history of genetic invasion suffered by a bacterial strain. To understand better the role of domestic dogs in human health as an antimicrobial resistance genes source, we analyzed 129 genomes of Staphylococcus strains of canine origin for the presence of CRISPR systems. Only 8% of the strains were positive for CRISPR, which is consistent with Staphylococcus role as gene reservoirs. The plasmidial origin or some spacers confirms the unsuccessful attempt of plasmid exchange in strains carrying CRISPRs. Some of these systems are within a staphylococcal cassette chromosome mec (SCCmec), sharing 98% of identity between their harboring strains. These CRISPRs' spacers reveal that this SCCmec was transferred between canine S. pseudintermedius strains, then to S. schleiferi and to Staphylococcus strains isolated from human beings. Our findings shows genetic evidence for the global spreading of pathogenic bacteria and the antimicrobial resistance genes carried by them and reinforce that, in the age of antimicrobial resistance, it is imperative that drug therapies consider the integrated nature of the relationship between pets and humans.
Assuntos
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Farmacorresistência Bacteriana/genética , Infecções Estafilocócicas/veterinária , Staphylococcus/genética , Animais , Reservatórios de Doenças/microbiologia , Cães/microbiologia , Transferência Genética Horizontal , Genoma Bacteriano , Humanos , Resistência a Meticilina/genética , Testes de Sensibilidade Microbiana , Animais de Estimação/microbiologia , Plasmídeos , Infecções Estafilocócicas/transmissão , Staphylococcus/isolamento & purificaçãoRESUMO
This study presented a biosafety device for the hygienic storage of composite resin during restorative procedures in teaching clinics, RESTAURASAFE, and to compare the color stability, surface hardness, and degree of conversion of the composite resin made with the proposed device to those of conventionally made resins. Esthet-X HD composite was manipulated directly from the syringe, fractionated doses, dappen dish or RESTAURASAFE. Disc-shaped specimens were immersed in artificial saliva and coffee for 60 days and the color stability and the Vickers surface hardness were evaluated during 60 days. Degree of conversion was assessed after immersing specimens in saliva (baseline). The surface morphology analysis of composite resin has taken by scanning electron microscopy (SEM). The data were evaluated statistically by repeated measures ANOVA (α=.05). No difference of color stability was observed for saliva and coffee regardless of storing devices. The resin manipulated directly from the syringe presented lower hardness, and fractionated doses presented lower degree of conversion than the RESTAURASAFE. SEM analysis showed that after artificial aging in coffee for 60 days, the specimens prepared with fractionated doses of resin displayed the fewest changes in surface morphology, followed by those prepared with the RESTAURASAFE. We conclude that RESTAURASAFE allows to obtain similar color stability, higher hardness, and degree of conversion of the composite in comparison to the other storing devices.
Assuntos
Resinas Compostas/química , Restauração Dentária Permanente/métodos , Controle de Infecções/métodos , Teste de Materiais/métodos , Resinas Vegetais/química , Café/química , Dureza/efeitos dos fármacos , Humanos , Saliva/química , Propriedades de SuperfícieRESUMO
Objective: The aim of this study was to evaluate the effect of different concentrations of a natural antioxidant (grape seed extract) on the bond strength of the restorative material to the bleached enamel. Methods: Forty fragments of healthy bovine incisors were randomly divided into four groups (n = 10): Group I: no bleaching; Group II: Bleaching with 35% hydrogen peroxide (HP) and without post-treatment; Group III: Bleaching with 35% HP + 5% grape seed extract; and Group IV: Bleaching with 35% HP + 10% grape seed extract. The bond strength at the adhesive interface was evaluated using the shear test (MPa). The data were analyzed by the analysis of variance (ANOVA) and Tukey test ( =0.05%). The fracture types were also analyzed and classified into: adhesive, cohesive or mixed. Results: Only Group III (bleached + 5% grape seed extract) had a significant increase (p<0.001) in bond strength values when compared to Group II bleached, without post- reatment). All groups showed a predominance of the adhesive type of fracture. Conclusion: It could be concluded that tooth bleaching decreases the bond strength to bleached enamel and 5% grape seed extract applied after dental bleaching improves the bond strength between the restorative material and the bleached enamel.
Objetivo: Este trabalho teve por objetivo avaliar in vitro o efeito de um antioxidante natural (extrato de semente de uva) em diferentes concentrações, na resistência de união do material restaurador ao esmalte clareado. Métodos: Quarenta fragmentos de incisivos bovinos hígidos, foram divididos aleatoriamente em quatro grupos (n=10): Grupo I: sem clareamento; Grupo II: clareado com peróxido de hidrogênio 35% (PH) e sem pós-tratamento; GIII: clareado PH 35% + extrato de semente de uva 5%; e Grupo IV: clareado com PH 35% + extrato de semente de uva 10%. A resistência de união da interface esmalte/material restaurador foi avaliada por meio do teste de cisalhamento (MPa). Os dados foram analisados pela análise de variância (ANOVA) e testes de Tukey ( =0,05%). Os tipos de fratura também foram analisados e classificados em: adesiva, coesiva ou mista. Resultados: Apenas o Grupo III (clareado + extrato de semente de uva 5%) apresentou aumento estatisticamente significante (p<0,001). dos valores de resistência de união comparado ao Grupo II (clareado e sem pós-tratamento). Todos os grupos mostraram um predomínio do tipo de fratura adesiva. Conclusão: O clareamento dental diminui significativamente a força de adesão ao esmalte dental clareado, e o extrato de semente de uva 5% aplicado após o clareamento dental melhora a resistência de união entre o material restaurador e o esmalte clareado.
Assuntos
Bovinos , Clareamento Dental , Técnicas In Vitro , Esmalte Dentário , Extrato de Sementes de Uva , AntioxidantesRESUMO
Aim: This study aims to compare masticatory performance between total prosthesis users and individuals with partial or total natural dentition to evaluate masticatory capacity and efficiency in individuals in Araraquara/SP. Material and Methods: 31 individuals between 55 and 99 years old were evaluated, both genders, frequent users of Dentistry Clinic of the University of Araraquara (Uniara) and residents at Recanto Feliz, in the same town. A cognitive evaluation was carried out through a mini mental state examination in order to define inclusion criteria to individuals of sample. Next, a questionnaire was applied, with pre-coded questions on the masticatory capacity and an efficiency test, through the grinding test, where patients received 5 almonds to be grinded and not swallowed. The fragments were poured in granulometric sieves (2mm, 4mm and 4,75mm) to analyze the food fragmentation under tap water. Participants were divided into 3 groups: G1 (n=13) superior and inferior total prosthesis user, or total or partial edentulous; G2 (n=4) Total prosthesis user and/or partial removal prosthesis user (PRP) or partial dentition; and G3 (n=14) complete or partial dentition or PRP user. Results: Efficiency test results showed the G1 patients presented good efficiency and 8 were considered poor; G2 patients were all classified as poor, and G3 patients were 9 poor, 2 regular and 3 good. Regarding to the questionnaires, most patients from the 3 groups felt impossibility to masticate some food, and need special preparation to chew. On take more time to masticate when compared to other people, all patients from G2 answered affirmatively, while only 46.1% G1 and 50% G3 gave the same answer.Just 15.4% G1 and 28.6% G2 are not satisfied with the tame they take.About avoid in front of other people, most patients from G2 and G3 use to, however, 69.2% G1 answered negatively. Regarding to the masticatory capacity self-evaluation, they answered regular. Conclusions: Masticatory efficiency of people using removable prosthesis did not achieve the ideal of a complete dentition. However, it is still better than in individuals with partial dentition or not rehabilitated.
RESUMO
Abstract Visual-motor maturity and executive functions are closely related in the child development process. This study aimed to investigate the relation between visual-motor abilities and executive functions in 83 healthy children between 7 and 10 years old. The tools used were the Bender Gestalt Visual-Motor Test - Gradual Scoring System (B-GSS), Wisconsin Card Sorting Test (WCST), Raven's Progressive Matrices (RPM), and Rey-Osterrieth Complex Figure (ROCF). The correlation between the B-GSS and WCST scores was significantly negative (r = -.23, p < .033), while ROCF variables, such as Total Memory and Total Copy, had a moderate, significant correlation with total B-GSS score (r = -.55, p < .001; r = -.44, p < .001, respectively). The results empirically show the relation between executive functions and visual-motor maturity and are discussed in face of developmental neuropsychology.(AU)
Resumo A maturidade visomotora e as funções executivas estão intimamente relacionadas no processo de desenvolvimento infantil. O objetivo deste estudo foi investigar a relação entre habilidades visomotoras e funções executivas em 83 crianças hígidas, de 7 a 10 anos. Os instrumentos utilizados foram Teste Gestáltico Visomotor de Bender - Sistema de Pontuação Gradual (B-GSS), Teste Wisconsin de Classificação de Cartas (WCST), Matrizes Progressivas de Raven (RPM) e Rey- Osterrieth Figura Complexa Test (ROCF). A correlação entre os escores de B-GSS e WCST foi negativa e significativa (r = 0,23, p < 0,033) e variáveis do ROCF, como Cópia Total e Memória Total apresentaram correlação moderada e significativa com o escore total do B-GSS (r = -0,55, p < 0,001; r = -0,44, p < 0,001, respectivamente). Os resultados evidenciam empiricamente a relação entre funções executivas e maturidade visomotora e são discutidos à luz da neuropsicologia do desenvolvimento.(AU)
Resumen La madurez visual-motora y las funciones ejecutivas están estrechamente relacionados en el proceso de desarrollo del niño. El objetivo de este estudio fue investigar la relación entre habilidades visomotoras y funciones ejecutivas en 83 niños sanos, de 7-10 años. Los instrumentos utilizados fueron Prueba Gestáltica Visomotora de Bender - Sistema de Puntuación Gradual (B-GSS), Prueba Wisconsin de Clasificación de Cartas (WCST), Matrices Progresivas de Raven (RPM) y Prueba Rey- Osterrieth Figura Compleja (ROCF). La correlación entre las puntuaciones B-GSS y WCST fue negativa y significativa (r = 0,23, p < 0,033) y ROCF variables, como Copiar y Memoria Total mostraron correlación moderada y significativa con la puntuación total de la B-GSS (r = -0,55, p < 0,001; r = -0,44, p < 0,001, respectivamente). Los resultados demuestran empíricamente la relación entre las funciones ejecutivas y la madurez visomotora y se discuten a la luz de la neuropsicología del desarrollo.(AU)
Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Terapia Cognitivo-Comportamental , Neuropsicologia , Teste de Bender-GestaltRESUMO
Visual-motor maturity and executive functions are closely related in the child development process. This study aimed to investigate the relation between visual-motor abilities and executive functions in 83 healthy children between 7 and 10 years old. The tools used were the Bender Gestalt Visual-Motor Test - Gradual Scoring System (B-GSS), Wisconsin Card Sorting Test (WCST), Raven's Progressive Matrices (RPM), and Rey-Osterrieth Complex Figure (ROCF). The correlation between the B-GSS and WCST scores was significantly negative (r = -.23, p < .033), while ROCF variables, such as Total Memory and Total Copy, had a moderate, significant correlation with total B-GSS score (r = -.55, p < .001; r = -.44, p < .001, respectively). The results empirically show the relation between executive functions and visual-motor maturity and are discussed in face of developmental neuropsychology.
A maturidade visomotora e as funções executivas estão intimamente relacionadas no processo de desenvolvimento infantil. O objetivo deste estudo foi investigar a relação entre habilidades visomotoras e funções executivas em 83 crianças hígidas, de 7 a 10 anos. Os instrumentos utilizados foram Teste Gestáltico Visomotor de Bender - Sistema de Pontuação Gradual (B-GSS), Teste Wisconsin de Classificação de Cartas (WCST), Matrizes Progressivas de Raven (RPM) e Rey- Osterrieth Figura Complexa Test (ROCF). A correlação entre os escores de B-GSS e WCST foi negativa e significativa (r = 0,23, p < 0,033) e variáveis do ROCF, como Cópia Total e Memória Total apresentaram correlação moderada e significativa com o escore total do B-GSS (r = -0,55, p < 0,001; r = -0,44, p < 0,001, respectivamente). Os resultados evidenciam empiricamente a relação entre funções executivas e maturidade visomotora e são discutidos à luz da neuropsicologia do desenvolvimento.
La madurez visual-motora y las funciones ejecutivas están estrechamente relacionados en el proceso de desarrollo del niño. El objetivo de este estudio fue investigar la relación entre habilidades visomotoras y funciones ejecutivas en 83 niños sanos, de 7-10 años. Los instrumentos utilizados fueron Prueba Gestáltica Visomotora de Bender - Sistema de Puntuación Gradual (B-GSS), Prueba Wisconsin de Clasificación de Cartas (WCST), Matrices Progresivas de Raven (RPM) y Prueba Rey- Osterrieth Figura Compleja (ROCF). La correlación entre las puntuaciones B-GSS y WCST fue negativa y significativa (r = 0,23, p < 0,033) y ROCF variables, como Copiar y Memoria Total mostraron correlación moderada y significativa con la puntuación total de la B-GSS (r = -0,55, p < 0,001; r = -0,44, p < 0,001, respectivamente). Los resultados demuestran empíricamente la relación entre las funciones ejecutivas y la madurez visomotora y se discuten a la luz de la neuropsicología del desarrollo.
Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Teste de Bender-Gestalt , Terapia Cognitivo-Comportamental , NeuropsicologiaRESUMO
AIM: This study aimed to report a case in which the technique used was the atraumatic Restorative Treatment (ART) in patients with fear of conventional treatment. MATERIAL AND METHODS: This procedure consisted in the treatment of carious lesions in the patient associated with the program of educational and preventive measures which the patient has undergone. The technique described here was performed on tooth 85, which presented atypical carious lesion cavity, through the removal of softened carious tissue with the use of hand instruments, without anesthesia and use of direct restorative material with fluoride release (glass ionomer cement). RESULTS: As a result of this technique, there was greater patient cooperation and decrease of anxiety initially revealed. CONCLUSION: Thus, it might be concluded that the Atraumatic Restorative Treatment is a technique already established that showed clinical effectiveness in controlling the evolution of the carious process and that being well indicated, was able to induce more cooperation from the patient, leading to the success of therapy restorer. The conventional glass ionomer cement showed excessive surface wear and therefore should not be used to restore atypical cavities.
RESUMO
O presente estudo teve por objetivo investigar a epidemiologia do câncer bucal no Brasil, por meio da revisão de literatura publicada nos últimos anos. Com isso, pretendeu-se descrever o perfil dos pacientes diagnosticados e susceptíveis ao câncer bucal, e ressaltar a importância da prevenção e diagnóstico precoce das lesões suspeitas. Observou-se que o perfil epidemiológico dos indivíduos mais acometidos pelo câncer de boca é caracterizado por homens, brancos, na faixa etária entre 50 e 70 anos de idade, trabalhadores expostos ao sol, usuários crônicos de tabaco, álcool ou a associação destes; os indivíduos de maior risco podem ainda apresentar trauma crônico devido ao uso de prótese; a localização da lesão é, principalmente, no dorso da língua e no lábio inferior. Considerando o câncer bucal como um problema de saúde pública, maiores esforços devem ser despendidos para a prevenção e combate dessa doença na população
The aim of this study was to investigate the epidemiology of oral cancer in Brazil, through a review of literature published in recent years. Thus, it was described the profile of diagnosed and susceptible to oral cancer patients, and it was emphasized the importance of prevention and early diagnosis of suspicious lesions. It was observed that the epidemiological profile of individuals most affected by mouth cancer is characterized by white men, between 50 to 70 years old, workers exposed to the sunlight, chronic users of tobacco, alcohol or a combination of them. The high risk individuals may still have chronic trauma due to the use of prosthesis. The lesion is mainly on the back of the tongue and lower lip. Whereas oral cancer as a public health problem, greater effort should be made to prevent and combat this disease in the population
Assuntos
Neoplasias Bucais , Carcinoma de Células Escamosas , EpidemiologiaRESUMO
O presente trabalho é uma revisão da literatura com o objetivo de observar a transmissão de doenças infecciosas orais através do contato entre mãe e filho e a influência de medidas preventivas para evitar possíveis doenças. Por meio da literatura revisada pode-se observar uma "relação vertical" de transmissibilidade da cárie caracterizada pela transferência materna de microorganismos cariogênicos para os seus filhos; medidas preventivas, as quais reduzem o nível salivar de Streptococus mutans em mulheres, retardam a colonização em suas crianças por esses organismos; alertar para a necessidade do desenvolvimento de programas educativos voltados para os pais e preventivos voltados para os bebês, procurando estabelecer hábitos saudáveis para evitar a contaminação da cavidade bucal da criança e oferecer a verdadeira promoção de saúde. Concluiu-se que a transmissibilidade da cárie apresenta relação-vertical, tendo a mãe papel de destaque na infecção oral primária de seus filhos. Medidas educativas e preventivas junto às mães, desde o período gestacional, são alternativas plausíveis e viáveis para a manutenção de um equilíbrio satisfatório das condições orais na criança
The aim of this review of the literature is to observe the transmission of oral infectious disease between mother and son and the influence of prevention methods to prevent possible disease. It was observed a vertical transmission of caries characterized by the transference of microorganisms of the mother to her son. Prevention methods, which reduce the level of Streptococus mutans in women, delay the settling in children for these organisms. Educative and preventive programs for parents and their babies are necessary to establish healthful habits and to prevent the contamination of the child. It was concluded that the mother is responsible by primary oral infection of children. Educative and preventive methods direct to the mothers, since the period of pregnancy, are reasonable for the maintenance of a satisfactory balance of the oral conditions in the child