RESUMO
O uso da criobiologia tem sido importante ferramenta para a preservação dos gametas. Atualmente, um dos desafios da área consiste na preservação do tecido ovariano e testicular por tempo prolongado e a criobiologia tem se mostrado como parte inerente no processo. Embora existam relatos sobre a criopreservação de tecido ovariano e obtenção de nascimentos em diferentes espécies, a criopreservação de tecido testicular ainda encontra-se limitada ao nível da experimentação. O desafio é promover um protocolo de criopreservação capaz de manter o potencial do tecido de completar a espermatogênese. A criopreservação de tecido testicular pode vir a ser um método utilizado quando técnicas como a congelação do ejaculado não é possível.
The use of cryobiology has been extremely important for gametes preservation. Nowadays, the challenge is the study of long-term preservation of ovarian and testicular tissue and the use of cryobiology is an important tool in this process. Although ovarian cryopreservation has successfully produced live births in different species, cryopreservation of testicular tissue is still restricted to a limited experimental level. The challenge is to provide a successful cryopreservation protocol for testis tissue capable of maintaining the potential of the tissue for completion spermatogenesis. Cryopreservation of testicular tissues theoretically offers a practical method when other techniques such as cryopreservation of ejaculated sperm are not available or applicable.
Assuntos
Animais , Criopreservação , Criopreservação/métodos , Criopreservação/veterinária , Espermatogênese , Soluções para Preservação de ÓrgãosRESUMO
O uso da criobiologia tem sido importante ferramenta para a preservação dos gametas. Atualmente, um dos desafios da área consiste na preservação do tecido ovariano e testicular por tempo prolongado e a criobiologia tem se mostrado como parte inerente no processo. Embora existam relatos sobre a criopreservação de tecido ovariano e obtenção de nascimentos em diferentes espécies, a criopreservação de tecido testicular ainda encontra-se limitada ao nível da experimentação. O desafio é promover um protocolo de criopreservação capaz de manter o potencial do tecido de completar a espermatogênese. A criopreservação de tecido testicular pode vir a ser um método utilizado quando técnicas como a congelação do ejaculado não é possível.(AU)
The use of cryobiology has been extremely important for gametes preservation. Nowadays, the challenge is the study of long-term preservation of ovarian and testicular tissue and the use of cryobiology is an important tool in this process. Although ovarian cryopreservation has successfully produced live births in different species, cryopreservation of testicular tissue is still restricted to a limited experimental level. The challenge is to provide a successful cryopreservation protocol for testis tissue capable of maintaining the potential of the tissue for completion spermatogenesis. Cryopreservation of testicular tissues theoretically offers a practical method when other techniques such as cryopreservation of ejaculated sperm are not available or applicable.(AU)
Assuntos
Animais , Criopreservação/métodos , Criopreservação , Criopreservação/veterinária , Soluções para Preservação de Órgãos , EspermatogêneseRESUMO
Background: Fertility control of animals by nonsurgical methods has been pursued to prevent unwanted pregnancies and suppress testosterone-dependent traits in farm and companion animals. In the latter, injection of sclerosing agents into the testis had considerable potential as a candidate for chemical sterilization of dogs. This article aims to review the potential of this new non-surgical technology for sterilization of male dogs. Review: Investigations of contraceptive agents for male dogs where initiated only in the last decade. Chemical sterilization is a solution for pet overpopulation and for non-surgical methods of male contraception. A variety of compounds have been tested, some of which were either safe but not effective or vice versa. The ideal chemical sterilization needs to meet three key criteria to be regarded as a good alternative to surgical sterilization. First, it has to be effective in a high percentage of treated animals. Secondly, it should have a high margin of safety for treated animals and the environment. Third, it has to be permanent and irreversible following a single treatment. The first product obviously fulfilling these criteria was zinc gluconate. Conclusion: Intratesticular injection of a zinc gluconate-based solution has great potential as a permanent contraceptive for dogs.