RESUMO
A Multiple Myeloma (MM), IgG-lambda stage III-A was diagnosed in a 41-year-old-man. After VAD cycles IgG decreased from 7.5 to 2.4 g/dL. were mobilized with cyclophosphamide and 10 micrograms/Kg G-CSF. Three days after the collection of peripheral stem cell, the patient had fever, nausea, vomiting, liquid stools, shoulder and knee arthralgia and dehydration. Upper GI endoscopy showed esophageal candidiasis and ulcerative necrotic lesions both in stomach and duodenum; the biopsy confirmed necrosis. Simultaneously, the appearance of purpura with maculopapular lesions of diverse sizes appeared in the feet progressing to the limbs and trunk. Hematuria and proteinuria were also observed. Skin biopsy showed leukocytoclastic vasculitis. Renal biopsy showed focal and segmental glomerulonephritis. Serum ANCA, cryoglobulins, anti-HCV and RF were negative, and serum monoclonal IgG was 1290 mg/dL. Daily treatment with i.v. methylprednisolone pulses for 3 days improved skin lesions and digestive involvement. Macroscopic hematuria and proteinuria improved after two months of steroid treatment.
Assuntos
Vasculite por IgA/etiologia , Mieloma Múltiplo/complicações , Adulto , Humanos , Vasculite por IgA/patologia , Masculino , Mieloma Múltiplo/patologia , Paraproteinemias/diagnóstico , Paraproteínas/análiseAssuntos
Glutationa/análogos & derivados , Glutationa/metabolismo , Nifurtimox/farmacologia , Nitroimidazóis/farmacologia , Espermidina/análogos & derivados , Tripanossomicidas/farmacologia , Trypanosoma cruzi/fisiologia , Animais , Espermidina/metabolismo , Compostos de Sulfidrila/metabolismo , Trypanosoma cruzi/efeitos dos fármacosRESUMO
Two Herpes simplex viral serotypes, HSV type 1 and HSV type 2 may cause genital herpes. The aim of this study was to perform a genetical analysis and characterization of virus isolated from four patients with double genital herpetic infections. In eleven viral isolates, the cytopathic effect in Vero cells was studied, the antigenic type was determined using monoclonal antibodies and genomic analysis was performed with Eco RI, Hind III and Bgl II enzymes. Five viral isolates generated a diffuse and 6 a localized cytopathic effect. Monoclonal antibodies identified four HSV-1 and seven HSV-2. Genomic analysis had concordant results. Four HSV-1 were obtained, with different genomic patterns within them, three were different to the standard North American strain. The seven HSV-2 obtained had three different types of electrophoretic profiles, that were different to the standard North American strain. It is concluded that the genomic and antigenic analysis allowed the detection of herpetic genital infections caused by herpes virus type 1 and 2 in the same individual and the identification of herpes virus strains with distinct regional characteristics.
Assuntos
Antígenos Virais/imunologia , Genoma Viral , Herpes Genital/virologia , Simplexvirus/genética , Simplexvirus/imunologia , Feminino , Humanos , Imunofenotipagem , Simplexvirus/isolamento & purificaçãoRESUMO
The Frutillar 82, F nasal 90, F vaginal 90, Puente Alto 77, VMLB 79, and Bio Bio 92 isolates of bovine herpesvirus 1 (BHV-1) from Chile were analysed by Eco RI, Bam HI and Hind III restriction enzymes (RE). The Los Angeles (LA) strain was used as reference. The results indicated that the fingerprints of F nasal 90 and F vaginal 90 were identical to the three RE used, but they were different from the other isolates. Major differences were found for Puente Alto 77 and VMLB 79 isolates with Eco RI, and for Puente Alto 77 with Hind III compared with the other Chilean isolates. It is concluded that Chilean BHV-1 isolates obtained from diverse lesions and geographic locations are genomically different from each other and from the other strains described in the literature.
Assuntos
DNA Viral/análise , Herpesvirus Bovino 1/genética , Animais , Bovinos , Chile , Impressões Digitais de DNA , Feminino , Herpesvirus Bovino 1/classificação , Mucosa Nasal/virologia , Mapeamento por Restrição , Vagina/virologiaRESUMO
The paperwork objective is to analyze if the HPV infections are associated to Ca in situ (CIS). The cytologic, colposcopic and histologic examinations of women affected by CIS were revised. It was determined that a group of them were also infected by HPV. On them, it was determined the viral genotype by nucleic acids hybridization. The Toki score was also utilized.
Assuntos
Carcinoma in Situ/microbiologia , Infecções Tumorais por Vírus/diagnóstico , Neoplasias do Colo do Útero/microbiologia , Carcinoma in Situ/patologia , Feminino , Humanos , Papillomaviridae/isolamento & purificação , Infecções Tumorais por Vírus/patologia , Neoplasias do Colo do Útero/patologiaRESUMO
The objective of this study, is to demonstrate the presence of human virus papilloma proteins (HPV) in cervix biopsies, diagnosed histologically as virus infected. In 52.9% of the cases (20 biopsies) the relation between HPV and histological study was found.
Assuntos
DNA Viral/análise , Infecções Tumorais por Vírus/microbiologia , Doenças do Colo do Útero/microbiologia , Capsídeo/genética , Capsídeo/imunologia , Feminino , Humanos , Papillomaviridae/genética , Infecções Tumorais por Vírus/patologia , Doenças do Colo do Útero/patologiaAssuntos
Colo do Útero/microbiologia , Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/isolamento & purificação , Doenças dos Genitais Femininos/diagnóstico , Infecções Sexualmente Transmissíveis/diagnóstico , Adolescente , Adulto , Idoso , Criança , Infecções por Chlamydia/microbiologia , Feminino , Doenças dos Genitais Femininos/microbiologia , Humanos , Pessoa de Meia-Idade , Infecções Sexualmente Transmissíveis/microbiologiaRESUMO
Se investiga la presencia, nivel y tipo de anticuerpos anti-virus herpes simples 1 y 2 (HSV-1 y HSV-2) en 112 mujeres portadoras de neoplasia cervical y 48 portadores de otros tipos de neoplasias.En ambos grupos se detecto una distribucion semejante de anticuerpos anti HSV-1 (98% de seropositivas). En cambio, frente al HSV-2 la distribucion de anticuerpos es claramente diferente: un 82,1% de las pacientes con cancer cervico-uterino presentan anticuerpos anti HSV-2 frente a 54,1% de seropositivas en el grupo de pacientes control. Al analizar el tipo serologico predominante en las pacientes estudiadas se detecta un predominio de anticuerpos anti HSV-2 en 47,3% de las portadoras de cancer cervical, y en 31,2% de enfermas con otras neoplasias
Assuntos
Humanos , Feminino , Anticorpos Antivirais , Herpes Simples , Neoplasias do Colo do ÚteroAssuntos
Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Herpes Genital , Testes SorológicosAssuntos
Linhagem Celular , Receptores Imunológicos/análise , Receptores Virais/análise , Neoplasias do Colo do Útero , Adulto , Efeito Citopatogênico Viral , Vírus de DNA , Feminino , Humanos , Vírus de RNA , Neoplasias do Colo do Útero/análise , Neoplasias do Colo do Útero/imunologia , Cultura de VírusRESUMO
Se estudia el rango de sensibilidad viral de una nueva linea celular (AdGo) establecida a partir de material de biopsia de un cancer invasor de cuello uterino humano.Se ensayan tanto virus DNA (adeno tipos 1 y 2, herpes simplex tipos 1 y 2) como RNA (Echo 11, Coxsackie B5, Polio 1, Respiratorio Sincicial y Sarampion). En las infecciones virales se detectan efectos citopaticos caracteristicos como: policariocitosis, agregacion celular y lisis. Tanto el rango de sensibilidad viral como la naturaleza de los efectos citopaticos observados son semejantes a aquellos detectados al utilizar otras lineas celulares epiteliales. Mediante ensayo de receptores para inmunoglobulinas en la superficie de las celulas AdGo, propiedad que no se observa en lineas celulares investigadas (HeLa y HEp-2). Se discute un posible significado de esta observacion