RESUMO
Concentrations of total serum IgE were measured by an immunoenzymatic assay (Phadezym-PRIST) in 60 mexican onchocerciasis patients. In order to detect IgE antibodies against adult Onchocerca volvulus antigens, separately six onchocerciasis sera were depleted of IgE antibodies by using a mixture of Onchocerca gutturosa, Ascaris suum and Fasciola hepatica antigenic extracts coupled with sepharose 4B. Additionally, the sera were incubated with an adult O. volvulus antigenic extract coupled with sepharose 4B. The differences found between the IgE levels before and after incubation with heterologous antigens show that the median IgE value against O. volvulus adults antigens varies between 20 and 65 percent of the total serum IgE.
Assuntos
Anticorpos Anti-Helmínticos/análise , Imunoglobulina E/análise , Oncocercose/imunologia , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Técnicas Imunoenzimáticas , Masculino , MéxicoRESUMO
A group of 180 male Wistar rats was utilized to study the synthesis of antibodies against sheep red blood cells (SRBC) after the surgical extirpation of seminal vesicles at 8-10 days of age. Rats were randomly divided into three groups of 60 animals. Group 1 had the rats which seminal vesicles were removed surgically. The second group had the sham-operated controls which glands were exposed only and the incision was closed forthwith. Animals of group 3 were normal and nonoperated. Between 1-6 months of age the rats were immunized intraperitoneally with a SRBC suspension. Four days later the animals were sacrificed, the spleens were removed from the abdominal cavity and the lymphocyte suspensions were obtained after homogenization of the tissues. Adjusted splenic viable lymphocyte suspensions were mixed with SRBC and guinea pig serum to count the anti-erythrocyte hemolytic plaque forming cells, according to the modification of Cunningham of the previously described cell lytic assay of Jerne. The amounts of splenic lymphocytes synthesizing antibodies against SRBC in the rats without seminal vesicles were significantly lower (p less than 0.001) than that of the sham-operated animals, except at 30 days of age. Proportions of rats with a number of plaque forming cells (PFC) above the median value were significantly lower (p less than 0.001) in the group of rats without seminal vesicles. A X test was used to explore differences between the proportions observed in the three groups.(ABSTRACT TRUNCATED AT 250 WORDS)