RESUMO
PURPOSE: Cryptosporidiosis is a zoonotic infectious disease caused by the protozoan parasite Cryptosporidium spp., frequently found in several animal species, including bats. Several Cryptosporidium genotypes have been described in bats worldwide, suggesting that bats are infected by host-specific Cryptosporidium spp. To date, there are no published reports about Cryptosporidium spp. in bats from Colombia. Therefore, this study aimed to determine the presence and molecular diversity of Cryptosporidium spp. in Colombian bats. METHODS: A total of 63 gut samples from three bat species served for molecular detection of Cryptosporidium spp. 18S rDNA gene by qPCR. The sequenced amplicons were used in subsequent phylogenetic analyses to identify them as species or genotypes. RESULTS: Cryptosporidium spp. qPCR detection occurred in 9.5% (6/63) of bat intestines, and four sequences represented two new genotypes, called Cryptosporidium bat genotypes XIX and XX, were identified. CONCLUSIONS: This study describes the detection of two novel Cryptosporidium bat genotypes, in two species of bats from a region of Colombia, requiring further studies to determine the relationhip between Cryptosporidium and bats in Colombia.
Assuntos
Quirópteros , Criptosporidiose , Cryptosporidium , Animais , Criptosporidiose/parasitologia , Cryptosporidium/genética , Quirópteros/parasitologia , Colômbia/epidemiologia , Genótipo , Filogenia , Fezes/parasitologiaRESUMO
Pig farming contributes to the economic development of nations and supplies human food demand; however, it generates a large amount of organic waste which, if not managed properly, becomes a risk to the environment and human and animal health. Considering the relevance of composting and its usefulness for the use of waste, this study aimed to determine the global trends in the management of composting manure, mortality and other organic waste produced on pig farms over the last five years (2017-2022). Systematic search involved four databases: ISI Web of Science, Scopus, Ebsco and Scielo. Of the total findings, 56 articles were included in the review, further classified into 14 categories for their respective analysis: co-substrates/additives, microbial communities, antibiotic resistance, heavy metals, polycyclic aromatic hydrocarbons, microbiological/parasitological quality, phytopathogens, nitrogen transformation, bioinoculants, comparison/combination with other waste management techniques, factors affecting composting, swine mortality and plant growth promotion/phytotoxicity. The review exemplified the importance of swine mortality composting as an alternative for organic matter management in pig farms, considering that the process also includes manure, vegetable waste and wood chips, among others. Controlled factors throughout the process are a requirement to obtain a stable product with physicochemical and microbiological quality that complies with national and international regulations and that will be useful and safe for application on crops, ensuring environmental, animal, and human health.