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1.
J Cosmet Dermatol ; 23(10): 3256-3262, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38958454

RESUMO

BACKGROUND: Given the significant increase in the quantity of cosmetic procedures utilizing hyaluronic acid fillers, including in the nasal region, the initial evaluation of patients using high frequency ultrasound becomes a crucial instrument in evaluating and handling nonsurgical rhinoplasty. AIMS: The aim of this article is to introduce an assessment methodology for nasal filling guided by high frequency ultrasound. PATIENTS/METHODS: A prospective and single-center study was conducted with 12 Latin American patients. The patients underwent nasal filling with hyaluronic acid following high power ultrasound mapping. RESULTS AND CONCLUSIONS: In the evaluation of the GAIS scale, all patients reported improvement with the treatment. No infections, nodules, ischemia, or other relevant adverse effects were noted. Real-time ultrasound-guided filler techniques have been developed to reduce the risk of vascular compromise, confirming the distribution pattern of blood vessels. It's also crucial to visualize the cannula at the same moment as the vessels, even if the previous vascular mapping was performed. Therefore, the utilization of high frequency ultrasound can act as a pivotal tool in augmenting procedure safety.


Assuntos
Preenchedores Dérmicos , Ácido Hialurônico , Ultrassonografia de Intervenção , Humanos , Ácido Hialurônico/administração & dosagem , Ácido Hialurônico/efeitos adversos , Estudos Prospectivos , Preenchedores Dérmicos/administração & dosagem , Preenchedores Dérmicos/efeitos adversos , Feminino , Ultrassonografia de Intervenção/efeitos adversos , Adulto , Pessoa de Meia-Idade , Rinoplastia/métodos , Rinoplastia/efeitos adversos , Nariz/diagnóstico por imagem , Técnicas Cosméticas/efeitos adversos , Técnicas Cosméticas/instrumentação , Masculino
2.
Toxicol In Vitro ; 98: 105832, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38653437

RESUMO

Sickle cell disease (SCD) is a hereditary hemoglobinopathy, caused by a mutation at position 6 of the ß-globin chain and patients are frequently exposed to several blood transfusions in order to maintain physiological function. Transfusion blood bags are composed of PVC and phthalates (as DEHP) are often introduced to the material in order to confer malleability. In this sense, DEHP can easily elute to the blood and cause harmful effects. This study aimed to unravel DEHP effect on SCD patient's hemoglobin function. We found that HbS polymerization using whole erythrocytes is decreased by DEHP in ex vivo experiments and this effect might be mediated by the DEHP-VAL6 interaction, evaluated in silico. Isolated HbS exhibited less polymerization at low DEHP concentrations and increased polymerization rate at higher concentration. When analyzing the propensity to aggregate, HbS is more inclined to aggregate when compared to HbA due to the residue 6 mutation. Circular dichroism showed characteristic hemoglobin peaks for oxygenated HbS that are lost when oxygen is sequestered, and DEHP at higher concentration mildly recovers a peak close to the second hemoglobin one. Finally, by transmission electron microscopy we demonstrated that high DEHP concentration increased polymer formation with a more organized structure. These findings show for the first-time the beneficial effect of low-dose DEHP on HbS polymerization.


Assuntos
Anemia Falciforme , Dietilexilftalato , Eritrócitos , Hemoglobina Falciforme , Polimerização , Humanos , Anemia Falciforme/genética , Anemia Falciforme/metabolismo , Hemoglobina Falciforme/genética , Hemoglobina Falciforme/metabolismo , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Dietilexilftalato/toxicidade , Simulação por Computador
3.
Anemia ; 2024: 7924015, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38596654

RESUMO

SCD is a hereditary disorder caused by genetic mutation in the beta-globin gene, resulting in abnormal hemoglobin, HbS that forms sickle-shaped erythrocytes under hypoxia. Patients with SCD have endocrine disorders and it was described that 7% of these patients have clinical hypothyroidism. Recent studies have shown that mature erythrocytes possess TSH receptors. Thus, we aimed to assess the effects of TSH on SCD erythrocytes. The experiments were conducted using different concentrations of TSH (1, 2, 3, and 5 mIU/L). In HbS polymerization assay, erythrocytes were exposed to TSH in hypoxia to induce polymerization, and measurements were taken for 30 minutes. The deformability assay was made using Sephacryl-S 500 columns to separate deformable from nondeformable cells. Static adhesion test utilized thrombospondin to assess erythrocyte adhesion in the presence of TSH. TSH at all contractions were able to reduce polymerization of HbS and increase deformability. The static adhesion of erythrocytes at the lowest concentrations of 1 and 2 mIU/L were increased, but at higher contractions of 3 and 5 mIU/L, static adhesion was not modulated. The results suggest that TSH has potential involvement in the pathophysiology of sickle cell disease by inhibiting HbS polymerization, positively modulating deformability and impacting static adhesion to thrombospondin.

4.
Can J Physiol Pharmacol ; 101(12): 642-651, 2023 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36821840

RESUMO

The effects of endothelin-1 (ET-1) on erythrocytes from sickle cell disease (SCD) patients have been described, but mechanisms of ET-1 regarding primary erythrocyte functions remain unknown. ET-1 is a vasoconstrictor peptide produced by endothelial cells, and the expression of ET-1 is increased in SCD. The present study used ex vivo experiments with sickle cell erythrocytes, ET-1, and bosentan, a dual antagonist of ETA and ETB receptors. We performed a hemoglobin S (HbS) polymerization assay with three concentrations of ET-1 (1, 20, and 50 pg/mL) and bosentan (100 nmol/L). ET-1 increased HbS polymerization at all concentrations, and this effect was suppressed by bosentan. For the deformability assay, red blood cells (RBCs) were incubated on a Sephacryl column with the same concentrations of ET-1 and bosentan. ET-1 decreased deformability, and this effect was reversed by bosentan. To observe erythrocyte adhesion, ET-1 and bosentan were incubated with RBCs in thrombospondin-coated 96-well plate, which demonstrated that ET-1 decreased adhesion but that bosentan enhanced adhesion. We also assessed erythrocyte apoptosis and observed decreased eryptosis induced by ET-1, and these effects were inhibited bosentan. Thus, these findings demonstrated that ET-1 modulates HbS polymerization, erythrocyte deformability, adhesion to thrombospondin, and eryptosis, and these effects were suppressed or enhanced by bosentan.


Assuntos
Anemia Falciforme , Endotelina-1 , Humanos , Bosentana/farmacologia , Endotelina-1/metabolismo , Células Endoteliais/metabolismo , Polimerização , Sulfonamidas/farmacologia , Eritrócitos/metabolismo , Anemia Falciforme/tratamento farmacológico , Deformação Eritrocítica , Trombospondinas , Antagonistas dos Receptores de Endotelina/farmacologia , Receptores de Endotelina/metabolismo , Endotelinas
5.
Cell Biochem Biophys ; 80(4): 711-721, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36175813

RESUMO

Angiotensin II (Ang II) regulates blood volume and stimulates erythropoiesis through AT1 (ATR1) and AT2 (ATR2) receptors, found in multiple tissues, including erythrocytes. Sickle cell disease (SCD) patients present altered Ang II levels. Hemoglobin S polymerization, deformability and phosphatidylserine translocation are important features of mature erythrocytes, therefore, our hypothesis is Ang II affects these parameters and, if it does, what would be the influence of AT1R and AT2R on these effects. A polymerization assay (PA), deformability, and annexin V binding were performed in SCD erythrocytes samples adding Ang II, ATR1 antagonist (losartan or eprosartan), and ATR2 antagonist (PD123319). Through the PA test, we observed a dose-dependent polymerization inhibition effect when comparing Ang II to control. Losartan did not affect the level or the rate of Ang II inhibition, while PD123319 showed an increased level of protection against polymerization, and eprosartan brought levels back to control. Ang II was able to reduce the translocation of phosphatidylserine from the inner to the outer leaflet, a marker of eryptosis, in the presence of PD123319. Also, ATR1 showed a positive effect increasing deformability. Our data shows that ATR1 is important for maintenance of erythrocyte physiological function in SCD and for prolonging its life.


Assuntos
Anemia Falciforme , Losartan , Acrilatos , Angiotensina II/metabolismo , Angiotensina II/farmacologia , Anexina A5 , Eritrócitos/metabolismo , Hemoglobina Falciforme/metabolismo , Humanos , Imidazóis , Losartan/farmacologia , Fosfatidilserinas , Polimerização , Receptor Tipo 1 de Angiotensina/metabolismo , Tiofenos
6.
Ciênc. cuid. saúde ; 21: e58689, 2022.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1421222

RESUMO

RESUMO Objetivo: conhecer o itinerário percorrido pela família para a obtenção do diagnóstico da criança com necessidades especiais de saúde. Método: realizou-se estudo qualitativo em uma unidade de internação pediátrica do sul do Brasil. Utilizou-se como referencial metodológico a Grounded Theory. Participaram 16 familiares cuidadores. Os dados foram coletados em 2019 por entrevistas e submetidos à codificação aberta e axial. Resultados: as famílias percorreram um longo itinerário até o recebimento do diagnóstico da criança: detectaram alterações no seu estado geral, realizaram diversos exames, desconfiaram o diagnóstico por já ter outros com o mesmo na família, receberam informações dos profissionais da saúde acerca dos cuidados necessários e dos serviços especializados para o seu tratamento, apresentaram medo, pânico, negação e valorizaram a fé em Deus na esperança da melhora da criança. Considerações finais: o enfermeiro necessita desenvolver um processo educativo junto à família para que ela se sinta preparada e capaz de cuidar dessas crianças.


RESUMEN Objetivo: conocer el itinerario recorrido por la familia para la obtención del diagnóstico del niño con necesidades especiales de salud. Método: se realizó estudio cualitativo en una unidad de hospitalización pediátrica del sur de Brasil. Se utilizó como referencial metodológico la GroundedTheory. Participaron 16 familiares cuidadores. Los datos fueron recolectados en 2019 por entrevistas y sometidos a la codificación abierta y axial. Resultados: las familias recorrieron un largo itinerario hasta la recepción del diagnóstico del niño: detectaron alteraciones en su estado general; realizaron diversos exámenes; desconfiaron del diagnóstico por tener ya otros con el mismo en la familia; recibieron informaciones de los profesionales de la salud acerca de los cuidados necesarios y de los servicios especializados para su tratamiento; presentaron miedo, pánico y negación; y valoraron la fe en Dios en la esperanza de la mejora del niño. Consideraciones finales: el enfermero necesita desarrollar un proceso educativo con la familia para que ella se sienta preparada y capaz de cuidar a esos niños.


ABSTRACT Objective: to know the itinerary taken by the family to obtain the diagnosis of the children with special health needs. Method: a qualitative study was carried out in a pediatric inpatient unit in southern Brazil. Grounded Theory was used as a methodological reference. Sixteen (16) family caregivers participated. Data were collected in 2019 through interviews and submitted to open and axial coding. Results: the families traveled a long route until receiving the children's diagnosis: they detected changes in their general condition; performed several exams; they were suspicious of the diagnosis because other members of the family had already been diagnosed with the same conditions; received information from health professionals about the necessary care and specialized services for the treatment; showed fear, panic and denial; and they valued faith in God in the hope of improving of the children. Final considerations: nurses need to develop an educational process with the family so that they feel prepared and capable of taking care of these children.


Assuntos
Humanos , Masculino , Feminino , Criança , Exames Médicos , Doença Crônica , Enfermagem , Diagnóstico
7.
RGO (Porto Alegre) ; 70: e20220012, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1376104

RESUMO

ABSTRACT Objective: The purpose of this study was to clinically evaluate the effect of one-stage full-mouth ultrasonic debridement in the treatment of diabetic subjects with severe chronic periodontitis. Methods: Sixteen patients with uncontrolled diabetes (glycated hemoglobin ≥ 7%) and severe chronic periodontitis (at least 8 teeth with a probing depth and bleeding on probing were selected and randomly assigned to 2 treatment groups: control group (n=7) - quadrant-wise scaling and root planning; test group (n=9) - full-mouth ultrasonic debridement in one session of 45 minutes. The following outcomes were assessed: plaque index, gingival index, bleeding on probing, probing depth, clinical attachment level and gingival margin position. All parameters were assessed at baseline, 1 and 3 months after treatment. Probing depth, clinical attachment level and gingival margin position were analyzed using ANOVA and Tukey test, and the other clinical parameters analysed with the Friedman and Mann-Whitney tests. Results: An improvement in probing depth, clinical attachment level was observed after treatment in both groups, but without statistically significant differences between them. After 3 months, in initially deep pockets, the PD decreased 1.6 mm and 1.7 mm in test and control group, respectively. Clinical attachment level gain ≥ 2 mm was statistically significant at sites in control (10.1%) and test (13,4%) groups in 3 months. Conclusions: One-stage full-mouth ultrasonic debridement promoted similar clinical outcomes to those obtained with the scaling and root planning in the treatment of diabetic subjects with severe chronic periodontitis.


RESUMO Objetivo: Avaliar clinicamente o efeito do debridamento ultrassônico de boca toda no tratamento da periodontite crônica severa em pacientes diabéticos. Métodos: Dezesseis pacientes diabéticos descompensados (Hemoglobina Glicada ≥ 7%) com periodontite crônica severa (mínimo de 8 dentes com profundidade de sondagem ≥ 5 mm e sangramento à sondagem) separados aleatoriamente em 2 grupos: Controle (7): raspagem e alisamento radicular por quadrante e Teste (9): debridamento ultrassônico sessão única de 45 minutos. Os parâmetros clínicos avaliados foram: Índice de placa, Índice gengival, Sangramento à Sondagem, Profundidade de Sondagem, Nível de inserção clínico e Posição da Margem Gengival. As variáveis foram avaliadas no início, 1 e 3 meses após o tratamento. Para análise das variáveis quantitativas (Profundidade de sondagem, Nível de inserção e Posição da margem gengival de bolsas moderadas) foram realizados ANOVA e teste de Tukey e para as demais foram utilizados os testes de Friedman e Mann-Whitney. Resultados: Observou-se melhora na profundidade de sondagem e nível de inserção clínica após o tratamento em ambos os grupos, sem diferenças estatisticamente significativas entre eles. Após 3 meses, nas bolsas inicialmente profundas, a profundidade de sondagem reduziu 1,6 e 1,7 mm nos grupos teste e controle, respectivamente. Houve um ganho estatisticamente significante no nível de inserção ≥ 2 mm nos grupos controle (10,1%) e teste (13,4%), aos 3 meses. Conclusão: O debridamento ultrassônico de boca toda promoveu resultados clínicos semelhantes aos obtidos com a raspagem e alisamento radicular no tratamento da periodontite crônica severa em indivíduos diabéticos.

8.
Rep Biochem Mol Biol ; 10(2): 314-326, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34604421

RESUMO

BACKGROUND: Angiotensin II regulates blood volume via AT1 (AT1R) and AT2 (AT2R) receptors. As cell integrity is an important feature of mature erythrocyte, we sought to evaluate, in vitro, whether angiotensin II modulates resistance to hemolysis and the signaling pathway involved. METHODS: Human blood samples were collected and hemolysis assay and angiotensin II signaling pathway profiling in erythrocytes were done. RESULTS: Hemolysis assay created a hemolysis curve in presence of Ang II in several concentrations (10-6 M, 10-8 M, 10-10 M, 10-12 M). Angiotensin II demonstrated protective effect, both in osmotic stressed and physiological situations, by reducing hemolysis in NaCl 0.4% and 0.9%. By adding receptors antagonists (losartan, AT1R antagonist and PD 123319, AT2R antagonist) and/or signaling modulators for AMPK, Akt/PI3K, p38 and PKC we showed the protective effect was enhanced with losartan and abolished with PD 123319. Also, we showed activation of p38 as well as PI3K/Akt pathways in this system. CONCLUSION: Ang II protects human erythrocytes from hypo-osmotic conditions-induced hemolysis by activating AT2 receptors and triggering intracellular pathways.

9.
Psychiatry Res ; 288: 112977, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32302818

RESUMO

BACKGROUND: According to UNESCO's monitoring, more than 160 countries implemented nationwide closures, which impacted over 87% of the world's student population. Several other countries implemented localized school closures; should these closures become nationwide, millions of additional learners will experience education disruption. Universities from around the world have been uncertain about how long the coronavirus crisis will last and how it might affect the mental health of students and faculty. The psychological impact has been a critical disruptor, creating anxiety and uncertainty. METHOD: The data were cross-checked with information from the main international newspapers. RESULTS: By discussing online and distance education, the coronavirus opens an important and urgent issue that affects mental health - these are virtually unexplored topics, and their results have not been validated yet. Online education is not limited to distance education, as it regards a grouping of learning/teaching procedures completed in cyberspace. Blended learning was, thus, introduced as a tool in personalized learning to adjust to new realities. These are unprecedented circumstances, and we understand they create stress, favoring anguish and a fierce search for new knowledge acquisition. CONCLUSIONS: Current research highlights that anxiety and depression, exacerbated by uncertainties and intensification of the information flow, will grow extensively. Negative physiological consequences of stress will manifest. For instance, loneliness, which will increase under these circumstances, seems to have a negative impact on education and, therefore, on psychological pain and suffering.


Assuntos
Infecções por Coronavirus/psicologia , Educação a Distância , Saúde Mental , Pneumonia Viral/psicologia , Estudantes/psicologia , Adolescente , Ansiedade , Betacoronavirus , COVID-19 , Coronavirus , Infecções por Coronavirus/epidemiologia , Depressão , Humanos , Pandemias , Pneumonia Viral/epidemiologia , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave , SARS-CoV-2 , Incerteza , Universidades , Adulto Jovem
10.
Rev. Enferm. UERJ (Online) ; 27: e41031, jan.-dez. 2019.
Artigo em Português | BDENF - Enfermagem, LILACS | ID: biblio-1024417

RESUMO

Objetivo: conhecer as repercussões do diagnóstico da Sífilis Congênita no recém-nascido para a mãe. Método: pesquisa qualitativa desenvolvida com 15 mães de recém-nascidos com Sífilis Congênita e internados em um hospital público do sul do Brasil. Os dados foram coletados por entrevistas semiestruturadas, submetidos à análise de conteúdo e aprovados pelo Comitê de Ética em Pesquisa. Resultados: as mães manifestaram sentimentos de culpa, desespero, tristeza e horror. Havia esperança de não passar a infecção para o recém-nascido. Verificou-se a reincidência da doença em mais de uma gestação. Referiram medo do estigma social e buscaram informações acerca da doença na internet, como também com médicos e enfermeiros. Conclusão: há desinformação das mães quanto à infecção da sífilis, principalmente sobre como evitar a transmissão vertical e a reinfecção. Cabe ao enfermeiro, instrumentalizar a mãe para o cuidado ao recém-nascido com Sífilis Congênita, por meio de um processo educativo que qualifique o cuidado da criança e da mãe


Objective: to learn how a diagnosis of congenital syphilis in newborns affects the mothers. Method: in this qualitative study of 15 mothers of newborns with congenital syphilis admitted to a public hospital in southern Brazil, data were collected by semistructured interview, and treated by content analysis. The study was approved by the research ethics committee. Results: the mothers expressed feelings of guilt, despair, sadness and horror. They hoped not to transmit the infection to the newborn. The disease was found to have recurred in more than one pregnancy. They reported fear of social stigma, and looked for information about the disease on the Internet, as well as from doctors and nurses. Conclusion: mothers are misinformed regarding infection by syphilis, especially on how to prevent vertical transmission and reinfection. It is up to nurses to instruct mothers on how to care for newborns with congenital syphilis, through an educational process to improve care for the child and the mother.


Objetivo: conocer las repercusiones, para la madre, del diagnóstico de sífilis congénita en el neonato. Método: investigación cualitativa desarrollada junto a 15 madres de neonatos con sífilis congénita e ingresados en un hospital público en el sur de Brasil. Se recolectaron los datos a través de entrevistas semiestructuradas y se sometieron al análisis de contenido; después fueron aprobados por el Comité de Ética en Investigación. Resultados: las madres expresaron sentimientos de culpa, desesperación, tristeza y horror. Había esperanza de no transmitir la infección al neonato. Se verificó la reincidencia de la enfermedad en más de un embarazo. Declararon el temor al estigma social y buscaron información sobre la enfermedad en internet, así como junto a médicos y enfermeros. Conclusión: las madres están desinformadas en cuanto a la infección por Sífilis, especialmente sobre cómo prevenir la transmisión vertical y la reinfección. Le toca al enfermero darle a la madre herramientas para que cuide bien al neonato con Sífilis Congénita, a través de un proceso educativo que califique el cuidado del niño y la madre


Assuntos
Humanos , Feminino , Recém-Nascido , Adulto , Pessoa de Meia-Idade , Sífilis Congênita , Sífilis Congênita/diagnóstico , Sífilis Congênita/psicologia , Recém-Nascido , Transmissão Vertical de Doenças Infecciosas , Cuidados de Enfermagem , Brasil , Família , Pesquisa Qualitativa , Relações Mãe-Filho
11.
Rev. Enferm. UERJ (Online) ; 27: e42264, jan.-dez. 2019.
Artigo em Português | BDENF - Enfermagem, LILACS | ID: biblio-1046225

RESUMO

Objetivo: conhecer as dificuldades encontradas pela família para o cuidado à criança/adolescente com HIV. Metodologia: pesquisa qualitativa realizada em um Hospital Dia no sul do Brasil em 2018. Participaram 20 familiares cuidadores de crianças/adolescentes soropositivas. Os dados foram coletados por entrevistas e submetidos à Análise de Conteúdo. Respeitaram-se os princípios éticos. Resultados: as famílias afirmaram não ter facilidades no cuidado. Referiram dificuldades em manter o sigilo do diagnóstico para a criança/adolescente; na adesão desta à medicação e minimização dos seus efeitos colaterais, em seguir os horários de administração; na assiduidade às consultas; em sofrer preconceito e na aceitação da sociedade diante da doença, incluindo a preocupação com o futuro da criança/adolescente. Conclusão: verificou-se como importante a atuação dos profissionais da saúde/ enfermeiros investindo em uma assistência voltada para a promoção de saúde de crianças e adolescentes com HIV, promovendo educação em saúde e o manejo das dificuldades enfrentadas com o cuidado.


Objective: to learn the difficulties encountered by families in caring for children or adolescents with HIV. Methodology: this qualitative study was conducted at a day hospital in southern Brazil in 2018. The participants were 20 family caregivers of seropositive children or adolescents. Data were collected through interviews and subjected to Content Analysis. Ethical principles were respected. Results: the families claimed to have no care facilities. They reported difficulties in keeping the children's or adolescents' diagnosis confidential; securing adherence to medication and administration schedules; minimizing side effects; attending appointments; suffering prejudice and gaining social acceptance of the disease, which included concern for the children's or adolescents' futures. Conclusion: it was found to be important that health professionals' or nurses' invest in care focused on promoting the health of children and adolescents with HIV, furthering health education and managing the difficulties encountered in care.


Objetivo: conocer las dificultades encontradas por la familia para el cuidado al niño/adolescente con VIH. Método: investigación cualitativa realizada en un Hospital Día en el sur de Brasil en 2018. Participaron 20 familiares cuidadores de niños/adolescentes seropositivos. Los datos fueron recolectados por entrevistas y sometidos al Análisis de Contenido. Se respetaron los principios éticos. Resultados: las familias afirmaron no tener facilidades en el cuidado. Dijeron que tienen dificultades para mantener el secreto del diagnóstico junto al niño/adolescente, también para su adherencia a la medicación y minimización de sus efectos colaterales, para seguir los horarios correctos de administración; la asiduidad de las consultas; por sufrir con los prejuicios y la dificultad de aceptación social de la enfermedad y la preocupación por el futuro del niño/adolescente. Conclusión: se verificó que es muy importante la actuación de los profesionales de salud/enfermeros que invierten en una atención volcada a la promoción de salud de niños y adolescentes con VIH, promoviendo la educación sanitaria y la gestión de las dificultades enfrentadas con el cuidado.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Família , Criança , Saúde da Família , Adolescente , HIV , Enfermagem , Síndrome da Imunodeficiência Adquirida/psicologia , Pesquisa Qualitativa
12.
Rev. Enferm. UERJ (Online) ; 27: e40274, jan.-dez. 2019.
Artigo em Português | BDENF - Enfermagem, LILACS | ID: biblio-1049701

RESUMO

Objetivo: analisar a rede de apoio social utilizada pela família para cuidar a criança com paralisia cerebral. Método: pesquisa qualitativa, descritiva, realizada com 12 mães de crianças com paralisia cerebral atendidas em unidade de pediatria do Hospital Universitário de Rio Grande. Os dados foram coletados nos meses de agosto e setembro de 2018, por meio de entrevista semiestruturada, e submetidos à análise temática. Resultados: identificou-se a rede de apoio social formada pelos familiares, amigos, vizinhos, profissionais de saúde e ancorada pela fé em Deus. As mães foram protagonistas do cuidado e algumas não receberam nenhum tipo de apoio para os cuidados dos filhos. Conclusão: a rede de apoio social contribuiu para a superação de dificuldades da família e para o cuidado da criança, sendo fonte de apoio emocional, financeiro, ajuda com o transporte, remédios e orientação dos profissionais de saúde/enfermagem.


Objective: to know the social support network used by the family to care for children with cerebral palsy. Method: this qualitative and descriptive research was conducted with twelve mothers of children with cerebral palsy assisted at a pediatric unit of a University Hospital in Rio Grande. Data were collected in August and September 2018 through semi-structured interviews and submitted to thematic analysis. Results: we identified the social support network consisting of family, friends, neighbors, health professionals, and faith in God. Mothers were protagonists of care and some received no support for childcare. Conclusion: the social support network contributed to overcome family difficulties and childcare, being a source of emotional, financial support, help with transportation, medicine, and guidance from health/nursing professionals.


Objetivo: conocer la red de apoyo social utilizada por la familia para cuidar a niños con parálisis cerebral. Método: investigación cualitativa, descriptiva, realizada con doce madres de niños con parálisis cerebral atendidas en una unidad de pediatría de un Hospital Universitario de Río Grande. Los datos se recopilaron en agosto y septiembre de 2018 a través de entrevistas semiestructuradas y se sometieron a análisis temáticos. Resultados: identificamos que la red de apoyo social estaba formada por familiares, amigos, vecinos, profesionales de la salud y fe en Dios. Las madres fueron protagonistas de la atención y algunas no recibieron apoyo para el cuidado de los niños. Conclusión: la red de apoyo social contribuyó a superar las dificultades familiares y el cuidado de los niños, siendo una fuente de apoyo emocional y financiero, ayuda con el transporte, medicamentos y orientación de profesionales de la salud/enfermeros.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Apoio Social , Família , Paralisia Cerebral/prevenção & controle , Paralisia Cerebral/psicologia , Criança , Cuidado da Criança , Epidemiologia Descritiva , Pesquisa Qualitativa
13.
Acta sci. vet. (Online) ; 47(suppl.1): Pub. 430, Oct. 17, 2019. ilus
Artigo em Inglês | VETINDEX | ID: vti-25733

RESUMO

Background: Botulism is a non-febrile intoxication resulting from the ingestion of Clostridium botulinum neurotoxinsmanifested by partial or complete flaccid paralysis of the musculature of locomotion, swallowing and respiration. Theobjective of this study was to report the first case of botulinum intoxication associated with osteopathy in the state of Acre,as well as to alert breeders and veterinarians to the incidence of this disease in cattle farming.Case: The present report is an outbreak of botulism in the municipality of Acrelândia, in the state of Acre, which resultedin the death of 16 Nelore beef cattle in approximately 30 days. The affected animals were females in reproductive phasemaintained under extensive breeding system. The main clinical signs presented were weakness in the pelvic limbs, prostration, recumbency and death in less than 48 h. Only one animal, with similar symptomatology, was found alive and submitted to emergency therapeutic measures, but without success. During the necropsy of this bovine, no significant changeswere found, only related to the decubitus and agony time, except for fragments of long bones visualized in the reticulum.Samples of bone particles, ruminal contents, reticulum, rumen and intestine fragments were collected for the detection ofbotulinum toxins by the mouse bioassay method, as well as brain and brain stem for differential diagnosis of rabies andbovine spongiform encephalopathy by direct immunofluorescence and immunohistochemistry, respectively. The sampleswere sent to the Laboratory of General Bacteriology of the Biological Institute, São Paulo, Brazil, and all the analyzespresented negative results.Discussion: One of the main risk factors for the occurrence of botulinum...(AU)


Assuntos
Animais , Bovinos , Botulismo/patologia , Botulismo/veterinária , Doenças Ósseas/veterinária , Clostridium botulinum , Deficiência de Minerais , Toxina Tetânica
14.
J. nurs. health ; 9(2): 199206, abr.2019.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1047264

RESUMO

Objetivo: conhecer os sentimentos gerados na família pela internação hospitalar da criança. Métodos: estudo qualitativo, realizado no município do Rio Grande, com 21 familiares acompanhantes de crianças internadas, por meio de entrevista semiestruturada e dados tratados por análise temática. O estudo foi aprovado pelo Comitê de Ética em Pesquisa na Área de Saúde com o parecer favorável de número 92/2016. Resultados: verificaram-se que os familiares referiram medo do diagnóstico, desespero, preocupação, tristeza, ansiedade, desconforto e abatimento. Sentem-se mal e impotentes com a permanência da criança no hospital. Entretanto, entendem que nesse contexto serão fornecidos os recursos e o atendimento necessários. Considerações finais: o enfermeiro deve dar apoio e se fazer presente, desde o primeiro dia de internação da criança, a fim de auxiliar as famílias no cuidado mais qualificado à criança hospitalizada de forma harmônica.(AU)


Objective: to know the feelings generated in the families by hospitalization of the child. Methods: qualitative study carried out in the city of Rio Grande, with 21 family members of hospitalized children, through a semi structured interview and data treated by thematic analysis. The study was approved by the Research Ethics Committee in the Health Area with favorable opinion of number 92/2016. Results: family members reported fear of diagnosis, despair, worry, sadness, anxiety, discomfort and depression. They feel bad and helpless with the child's stay in the hospital. However, they understand that in this context, the necessary resources and assistance will be provided. Final considerations: nurses should provide support and be present, since the first day of hospitalization of the child, in order to help families with the most qualified care to the hospitalized child in a harmonic way.(AU)


Assuntos
Família , Criança Hospitalizada , Enfermagem
15.
Antibiotics (Basel) ; 8(1)2019 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-30884871

RESUMO

Orbignya speciosa (babassu) is an important palm tree in Brazil whose fixed almond oil is used in popular medicine and especially in food, in addition to being a research target for the manufacture of biofuels. The aim of this study was to evaluate the fixed almond oil physicochemical characterization and its antibacterial activity in isolation and in association with aminoglycosides against standard and multidrug-resistant bacteria. Analyses such as water content, pH, acidity, peroxide index, relative density, and refractive index indicate the stability and chemical quality of the oil. In the oil's GC/MS chemical composition analysis, a high saturated fatty acid (76.90%) content was observed. Lauric acid (56.28%) and oleic acid (23.10%) were the major oil components. In the antibacterial test, a more significant oil activity was observed against K. pneumoniae KP-ATCC 10031 (minimal inhibitory concentration (MIC) = 406.37 µg/mL) and Staphylococcus aureus ATCC 6538 (MIC = 812.75 µg/mL), but for the other strains-including standard and multi-resistant strains-the oil presented an MIC ≥ 1024 µg/mL. Furthermore, a synergistic effect was observed when the oil was associated with amikacin and gentamicin against S. aureus (SA-10) and an antagonistic effect was observed with amikacin against Escherichia coli. Data indicate the O. speciosa oil as a valuable nutritional source of lauric, oleic, and myristic fatty acids with an ability to modulate aminoglycoside activity.

16.
Diagnostics (Basel) ; 9(1)2019 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-30641881

RESUMO

The cell-free genomic DNA (gDNA) concentration in serum ranges from 1500 to 7500 copies/mL within 2 h after phlebotomy (6⁻24 times the concentration observed in plasma). Here, we aimed to evaluate the gDNA size distribution in serum with time after coagulation and to test if crude serum can be directly used as a source of gDNA for qPCR. Next, we investigated if single nucleotide polymorphisms (SNPs) could be genotyped directly from the crude serum isolated from capillary blood using a hand-powered paper centrifuge. All tested PCR targets (65, 100, 202 and 688 base pairs) could be successfully amplified from DNA extracted from serum, irrespective of their amplicon size. The observed qPCR quantitation cycles suggested that the genomic DNA yield increased in serum with incubation at room temperature. Additionally, only 65 and 101 base pair qPCR targets could be amplified from crude serum soon after the coagulation. Incubation for 4 days at room temperature was necessary for the amplification of PCR targets of 202 base pairs. The 688 base pair qPCR target could not be amplified from serum directly. Lastly, serum was successfully separated from capillary blood using the proposed paper centrifuge and the genotypes were assigned by testing the crude serum using allele-specific qPCR, producing small amplicon sizes in complete agreement with the genotypes assigned by testing the DNA extracted from whole blood. The serum can be used directly as the template in qPCR for SNP genotyping, especially if small amplicon sizes are applied. This shortcut in the SNP genotyping process could further molecular point-of-care diagnostics due to elimination of the DNA extraction step.

17.
J. nurs. health ; 9(1): 199108, jan. 8, 2019.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1029213

RESUMO

Objetivo: identificar as vivências do familiar frente ao diagnóstico de Diabetes Mellitus na criança/adolescente. Métodos: pesquisa qualitativa, realizada com 15 familiares de crianças/adolescentes do Centro Integrado de Diabetes de um Hospital Universitário do sul do Brasil. Coletou-se os dados no primeiro semestre de 2014 por entrevistas semiestruturadas, tratadas pela análise de Conteúdo. Resultados: os familiares identificam os sinais/sintomas, mas não associados com diabetes ou confundidos com outras patologias. O diagnóstico ocorre em consultas de rotina ou quando ocorre o agravamento da situação. Os sentimentos comuns são culpa e medo, por não saber como agir. Primeiro ocorre a negação e depois a busca por conhecimento acerca da doença. Considerações Finais: ao conhecer esse processo, os profissionais de enfermagem podem contribuir para diminuir o impacto do diagnóstico, apoiando a família e criança/adolescente no enfrentamento de mudanças.


Objective: to identify the experiences of the relative in the diagnosis of Diabetes Mellitus in the child/adolescent. Methods: qualitative research, carried out with 15 family members of children/adolescents from the Integrated Diabetes Center of a University Hospital in southern Brazil. Data was collected in the first half of 2014 through semi-structured interviews, handled by Content analysis. Results: family members identify the signs/symptoms, but they do not associate it with diabetes, or they are confused with other pathologies. The diagnosis occurs in routine consultations or when the situation worsens. Common feelings are guilt and fear, for not knowing how to act. First there is denial and then the search for knowledge about the disease. Final considerations: by knowing this process, nursing professionals can contribute to decrease the impact of diagnosis, supporting the family and child/adolescent in coping with changes.


Assuntos
Humanos , Adolescente , Criança , Diabetes Mellitus , Enfermagem , Família
18.
Acta sci. vet. (Impr.) ; 47(suppl.1): Pub.430-2019. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1458194

RESUMO

Background: Botulism is a non-febrile intoxication resulting from the ingestion of Clostridium botulinum neurotoxinsmanifested by partial or complete flaccid paralysis of the musculature of locomotion, swallowing and respiration. Theobjective of this study was to report the first case of botulinum intoxication associated with osteopathy in the state of Acre,as well as to alert breeders and veterinarians to the incidence of this disease in cattle farming.Case: The present report is an outbreak of botulism in the municipality of Acrelândia, in the state of Acre, which resultedin the death of 16 Nelore beef cattle in approximately 30 days. The affected animals were females in reproductive phasemaintained under extensive breeding system. The main clinical signs presented were weakness in the pelvic limbs, prostration, recumbency and death in less than 48 h. Only one animal, with similar symptomatology, was found alive and submitted to emergency therapeutic measures, but without success. During the necropsy of this bovine, no significant changeswere found, only related to the decubitus and agony time, except for fragments of long bones visualized in the reticulum.Samples of bone particles, ruminal contents, reticulum, rumen and intestine fragments were collected for the detection ofbotulinum toxins by the mouse bioassay method, as well as brain and brain stem for differential diagnosis of rabies andbovine spongiform encephalopathy by direct immunofluorescence and immunohistochemistry, respectively. The sampleswere sent to the Laboratory of General Bacteriology of the Biological Institute, São Paulo, Brazil, and all the analyzespresented negative results.Discussion: One of the main risk factors for the occurrence of botulinum...


Assuntos
Animais , Bovinos , Botulismo/patologia , Botulismo/veterinária , Clostridium botulinum , Doenças Ósseas/veterinária , Deficiência de Minerais , Toxina Tetânica
19.
Acta amaz. ; 48(4): 321-329, Oct.-Dec. 2018. mapas, tab, graf
Artigo em Inglês | VETINDEX | ID: vti-736292

RESUMO

Babassu, Attalea speciosa (Arecaceae) is a ruderal palm native to Amazonia, which turned dominant in frequently burned lands throughout the arc of deforestation and other degraded lands, in extreme cases attaining complete dominance. This study investigated arbuscular mycorrhizal fungi (AMF) as one possible explanation for the outstanding ecological success of this exceptional palm. We explored the relationships between the babassu palm and native arbuscular mycorrhizal fungi and babassu effects on the AMF richness and mycorrhizal inoculum potential (MIP) in the eastern periphery of Amazonia. For this purpose, we sampled topsoil (0-20 cm) at the onset of the rainy season from a 5-year-old secondary forest regrowth (SEC) area with three levels of babassu dominance (sites with 10, 50 and 70% babassu biomass shares), and at three distances (0, 2.5 and 4 m) from isolated babassu patches within a degraded pasture (PAS), both with five replications per treatment. Glomerospore density varied from 100 to 302 per gram of soil, 56% higher in SEC than PAS. We identified a total of 16 AMF species, with dominance of Acaulospora (six species) followed by Glomus (three species). AMF richness increased with babassu dominance in SEC sites, and reduced with distance from babassu patches within the PAS. The colonization rate of babassu roots was higher in SEC than in PAS, whereas MIP was similar in both areas and without treatment differences. Our study points to strong mycorrhizal association of the babassu palm as a potential mechanism for its outstanding ecological success in degraded lands.(AU)


Babaçu, Attalea speciosa (Arecaceae) é uma palmeira ruderal nativa da Amazônia, dominante em terras frequentemente queimadas ao longo do arco de desmatamento e outras áreas degradadas, em casos extremos atingindo domínio completo. Este estudo investigou os fungos micorrízicos arbusculares (FMA) como possível explicação do sucesso ecológico desta palmeira. Nós exploramos as relações entre o babaçu e glomerosporos, efeitos do babaçu na riqueza destes fungos e o potencial do inóculo micorrízico (PIM) na periferia oriental da Amazônia. Amostras de solo (0-20 cm) foram coletadas no início da estação chuvosa em uma área de floresta secundária (SEC) de cinco anos de idade e três níveis de dominância do babaçu (10, 50 e 70% de biomassa de babaçu) e a três distâncias (0; 2,5 e 4 m) de ilhas de babaçu isoladas em uma pastagem degradada (PAS), ambas com cinco repetições por tratamento. A densidade de esporos de FMA variou de 100 a 302 por grama de solo, sendo 56% maior em SEC do que em PAS. Dezesseis espécies de FMA foram identificadas, com predominância de Acaulospora (seis espécies) seguidos do gênero Glomus (três espécies). A riqueza destes fungos aumentou com o domínio da palmeira em SEC e reduziu com a distância das ilhas de babaçu em PAS. A taxa de colonização das raízes de babaçu foi superior nas áreas de SEC enquanto o PIM não apresentou diferenças entre os tratamentos. Nosso estudo aponta a uma forte associação micorrhízica da palmeira babaçu, um possível mecanismo central no seu sucesso ecológico em áreas degradadas.(AU)


Assuntos
Micorrizas/crescimento & desenvolvimento , Arecaceae/crescimento & desenvolvimento , Biodiversidade , Interações Microbianas , Ecossistema Amazônico , Brasil
20.
Acta amaz ; Acta amaz;48(4): 321-329, Oct.-Dec. 2018. map, tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1455376

RESUMO

Babassu, Attalea speciosa (Arecaceae) is a ruderal palm native to Amazonia, which turned dominant in frequently burned lands throughout the arc of deforestation and other degraded lands, in extreme cases attaining complete dominance. This study investigated arbuscular mycorrhizal fungi (AMF) as one possible explanation for the outstanding ecological success of this exceptional palm. We explored the relationships between the babassu palm and native arbuscular mycorrhizal fungi and babassu effects on the AMF richness and mycorrhizal inoculum potential (MIP) in the eastern periphery of Amazonia. For this purpose, we sampled topsoil (0-20 cm) at the onset of the rainy season from a 5-year-old secondary forest regrowth (SEC) area with three levels of babassu dominance (sites with 10, 50 and 70% babassu biomass shares), and at three distances (0, 2.5 and 4 m) from isolated babassu patches within a degraded pasture (PAS), both with five replications per treatment. Glomerospore density varied from 100 to 302 per gram of soil, 56% higher in SEC than PAS. We identified a total of 16 AMF species, with dominance of Acaulospora (six species) followed by Glomus (three species). AMF richness increased with babassu dominance in SEC sites, and reduced with distance from babassu patches within the PAS. The colonization rate of babassu roots was higher in SEC than in PAS, whereas MIP was similar in both areas and without treatment differences. Our study points to strong mycorrhizal association of the babassu palm as a potential mechanism for its outstanding ecological success in degraded lands.


Babaçu, Attalea speciosa (Arecaceae) é uma palmeira ruderal nativa da Amazônia, dominante em terras frequentemente queimadas ao longo do arco de desmatamento e outras áreas degradadas, em casos extremos atingindo domínio completo. Este estudo investigou os fungos micorrízicos arbusculares (FMA) como possível explicação do sucesso ecológico desta palmeira. Nós exploramos as relações entre o babaçu e glomerosporos, efeitos do babaçu na riqueza destes fungos e o potencial do inóculo micorrízico (PIM) na periferia oriental da Amazônia. Amostras de solo (0-20 cm) foram coletadas no início da estação chuvosa em uma área de floresta secundária (SEC) de cinco anos de idade e três níveis de dominância do babaçu (10, 50 e 70% de biomassa de babaçu) e a três distâncias (0; 2,5 e 4 m) de ilhas de babaçu isoladas em uma pastagem degradada (PAS), ambas com cinco repetições por tratamento. A densidade de esporos de FMA variou de 100 a 302 por grama de solo, sendo 56% maior em SEC do que em PAS. Dezesseis espécies de FMA foram identificadas, com predominância de Acaulospora (seis espécies) seguidos do gênero Glomus (três espécies). A riqueza destes fungos aumentou com o domínio da palmeira em SEC e reduziu com a distância das ilhas de babaçu em PAS. A taxa de colonização das raízes de babaçu foi superior nas áreas de SEC enquanto o PIM não apresentou diferenças entre os tratamentos. Nosso estudo aponta a uma forte associação micorrhízica da palmeira babaçu, um possível mecanismo central no seu sucesso ecológico em áreas degradadas.


Assuntos
Arecaceae/crescimento & desenvolvimento , Biodiversidade , Interações Microbianas , Micorrizas/crescimento & desenvolvimento , Brasil , Ecossistema Amazônico
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