RESUMO
Sepsis is a life-threatening condition and usually be treated with antibiotics, which however often has severe side effects. This work proposed a novel Chinese traditional medicine JINHONG (JH) decoction for therapy of sepsis. We first identified the chemical constituents of JH decoction by using high-performance liquid chromatography and mass spectrometry (HPLC-MS). Then, we constructed a model mouse for sepsis by using cecal ligation and puncture (CLP). Metagenomic sequencing method was used to compare the diversity and abundance of the gut microbiota between normal, disease model, JH decoction-treatment and antibiotic-treatment mice. Many indices including the number of platelets, CD62p and CD63 content, AQP2 and AQP8 level, as well as the expression level of protein C confirmed that the sepsis resulted in serious pathological damage, while all of these indices could be reversed by JH decoction and antibiotics. The diversity and abundance of intestinal flora decreased in CLP mice, and the decrements aggravated after antibiotic treatment while can be recovered by JH decoction treatment. The abundance of anti-inflammatory Ruminococcaceae increased after JH decoction treatment, indicating that JH decoction could ameliorate pathology associated with sepsis in CLP model via modulating the intestinal flora. This study demonstrates that JH decoction could treat sepsis clinically without obvious adverse effects on gut microbiota.
Assuntos
Modelos Animais de Doenças , Medicamentos de Ervas Chinesas , Microbioma Gastrointestinal , Sepse , Animais , Microbioma Gastrointestinal/efeitos dos fármacos , Sepse/tratamento farmacológico , Sepse/microbiologia , Medicamentos de Ervas Chinesas/farmacologia , Camundongos , Masculino , Antibacterianos/farmacologia , Cromatografia Líquida de Alta Pressão/métodos , Ceco/microbiologia , Camundongos Endogâmicos C57BLRESUMO
In order to analyze the scattering properties in multi-radiator problems, the active radar cross section (ARCS) concept is proposed under complex electromagnetic (EM) environments. The corresponding calculation methods and formulation are proposed by incorporating the monostatic radar cross section (RCS) concept with external disturbances. By introducing the phase characteristics into the ARCS concept, the coherent problems can be accurately solved. Through analyzing the external disturbance and the radar waves by employing the finite element method, the coherent and the incoherent characteristics of the external disturbance can be simulated in complex structures. Numerical examples and an experiment are carried out to further demonstrate the effectiveness of the proposed ARCS concept. The results demonstrate that the proposed ARCS concept obtains better universality compared with the existing incoherent multi-radiator formulation. Meanwhile, the ARCS can be identical with the solution which is obtained by the single radar wave. Compared with the existing incoherent methods for external disturbances calculations, the proposed ARCS concept is more rational. Through the experiment, the effectiveness of the calculation method and formulation is further demonstrated and validated.