RESUMO
Mozambique is one of the four African countries which account for over half of all malaria deaths worldwide, yet little is known about the parasite genetic structure in that country. We performed P. falciparum amplicon and whole genome sequencing on 2251 malaria-infected blood samples collected in 2015 and 2018 in seven provinces of Mozambique to genotype antimalarial resistance markers and interrogate parasite population structure using genome-wide microhaplotyes. Here we show that the only resistance-associated markers observed at frequencies above 5% were pfmdr1-184F (59%), pfdhfr-51I/59 R/108 N (99%) and pfdhps-437G/540E (89%). The frequency of pfdhfr/pfdhps quintuple mutants associated with sulfadoxine-pyrimethamine resistance increased from 80% in 2015 to 89% in 2018 (p < 0.001), with a lower expected heterozygosity and higher relatedness of microhaplotypes surrounding pfdhps mutants than wild-type parasites suggestive of recent selection. pfdhfr/pfdhps quintuple mutants also increased from 72% in the north to 95% in the south (2018; p < 0.001). This resistance gradient was accompanied by a concentration of mutations at pfdhps-436 (17%) in the north, a south-to-north increase in the genetic complexity of P. falciparum infections (p = 0.001) and a microhaplotype signature of regional differentiation. The parasite population structure identified here offers insights to guide antimalarial interventions and epidemiological surveys.
Assuntos
Humanos , Malária Falciparum/prevenção & controle , Malária/patologia , Antimaláricos/farmacologia , Humanos , Resistência a Medicamentos/genética , Malária Falciparum/terapiaRESUMO
Background: Prevention of reinfection and resurgence is an integral component of the goal to eradicate malaria. However, the adverse effects of malaria resurgences are not known. Methods: We assessed the prevalence of Plasmodium falciparum infection among 1819 Mozambican women who delivered infants between 2003 and 2012. We used microscopic and histologic examination and a quantitative polymerase-chain-reaction (qPCR) assay, as well as flow-cytometric analysis of IgG antibody responses against two parasite lines. Results: Positive qPCR tests for P. falciparum decreased from 33% in 2003 to 2% in 2010 and increased to 6% in 2012, with antimalarial IgG antibody responses mirroring these trends. Parasite densities in peripheral blood on qPCR assay were higher in 2010-2012 (geometric mean [±SD], 409±1569 genomes per microliter) than in 2003-2005 (44±169 genomes per microliter, P=0.02), as were parasite densities in placental blood on histologic assessment (50±39% of infected erythrocytes vs. 4±6%, P<0.001). The malaria-associated reduction in maternal hemoglobin levels was larger in 2010-2012 (10.1±1.8 g per deciliter in infected women vs. 10.9±1.7 g per deciliter in uninfected women; mean difference, -0.82 g per deciliter; 95% confidence interval [CI], -1.39 to -0.25) than in 2003-2005 (10.5±1.1 g per deciliter vs. 10.6±1.5 g per deciliter; difference, -0.12 g per deciliter; 95% CI, -0.67 to 0.43), as was the reduction in birth weight (2863±440 g in women with past or chronic infections vs. 3070±482 g in uninfected women in 2010-2012; mean difference, -164.5 g; 95% CI, -289.7 to -39.4; and 2994±487 g vs. 3117±455 g in 2003-2005; difference, -44.8 g; 95% CI, -139.1 to 49.5). Conclusions: Antimalarial antibodies were reduced and the adverse consequences of P. falciparum infections were increased in pregnant women after 5 years of a decline in the prevalence of malaria. (Funded by Malaria Eradication Scientific Alliance and others).
Assuntos
Humanos , Feminino , Gravidez , Adulto , Adulto Jovem , Complicações Infecciosas na Gravidez/epidemiologia , Imunoglobulina G/sangue , Malária Falciparum/imunologia , Malária Falciparum/epidemiologia , Paridade , Plasmodium falciparum/isolamento & purificação , Plasmodium falciparum/imunologia , Complicações Infecciosas na Gravidez/classificação , Complicações Infecciosas na Gravidez/imunologia , Índice de Gravidade de Doença , Anticorpos Antiprotozoários/sangue , Malária Falciparum/classificação , Carga Parasitária , Moçambique/epidemiologiaRESUMO
Vaccines are an effective public health measure. Vaccination coverage has improved in Africa in the last decades but has still not reached WHO/UNICEF target of at least 90% first-dose coverage for vaccines in the Expanded Programme on Immunization (EPI) implemented in Mozambique in 1979. There are concerns about reliability of vaccination coverage official data from low-income countries, and inequities in vaccine administration. We randomly sampled 266 under-five years children from Taninga, a poor rural area in Southern Mozambique under a Demographic surveillance system and collected data directly from the individual national health cards when available (BCG, DTP/HepB/Hib, Polio, Measles). We also collected data on socio-economic variables through an interview. Overall, only 5% of the participants did not receive all the doses of the vaccines included in the EPI in a timely manner (overall vaccination coverage 95%, 95% CI: 93.5-95.5%). The socio-economic status was homogenously low and no differences were found between vaccinated and unvaccinated children. Vaccination coverage in Taninga was very high, despite the low socio-economic status of the population. The high performance of the EPI in Taninga is an encouraging experience for achieving high vaccination coverage in low-income rural settings.