RESUMO
BACKGROUND: Zika virus is associated with increased cases of both microcephaly and Guillain-Barré syndrome. Community knowledge, perceptions and practices to prevent infection with the Zika virus are not well understood, particularly among high risk populations living in resource-poor and Zika-endemic areas. Our objective was to assess knowledge of symptoms, health effects and prevention practices associated with Zika virus in rural communities on the northern coast of the Dominican Republic. METHODS: Study participants were contacted while attending community events such as free medical clinics and invited to be interviewed regarding their knowledge, attitudes, and perceptions of Zika virus using the World Health Organization's Zika survey tool. RESULTS: Of the 75 Dominicans that participated, 33% did not know who could become infected with Zika. In addition, only 40% of respondents were able to identify mosquitoes or sexual transmission as the primary routes of infection though 51% of respondents thought that Zika was an important issue in their community. CONCLUSIONS: This study found that general knowledge regarding the basic risks and transmission of Zika were not well understood among a sample of rural Dominicans. Our findings highlight disparities in knowledge and perception of risk from Zika in rural areas compared to previous studies conducted in the Dominican Republic. Education about the basic risks and transmission of Zika are critically needed in these remote populations to reduce Zika transmission.
Assuntos
Conhecimentos, Atitudes e Prática em Saúde , População Rural/estatística & dados numéricos , Infecção por Zika virus , Zika virus , República Dominicana , Humanos , Infecção por Zika virus/psicologia , Infecção por Zika virus/transmissãoRESUMO
Sexual health education interventions have generally yielded modest impact, but may be more successful when integrated into programs designed to alleviate poverty and empower women. Between December 2017 and February 2018, we interviewed 304 Haitian female microfinance clients, 75 of whom had received health education training delivered within their regular meetings. Participants reported six key sexual health outcomes. We used log-binomial models to estimate the association between health education training and each outcome, and tested for interaction by age and literacy status. Women with health education training reported more condom use with unfaithful partners [PR (95% CI) 1.78 (1.04, 3.02)], more HIV testing [PR (95% CI) 1.56 (1.28, 1.90)], and fewer STI symptoms [PR (95% CI) 0.37 (0.19, 0.73)], compared to women with no training. Some of the associations were stronger among older women [e.g. HIV testing: PR (95% CI) 2.09 (1.49, 2.82)] and illiterate women [e.g. condom use: PR (95% CI) 3.46 (1.05, 11.38)]. These findings add to the growing body of evidence demonstrating the potential to use microfinance programs as platforms for health education delivery, and provide the first evidence for the association in Haiti.
Assuntos
Financiamento Pessoal , Infecções por HIV/economia , Infecções por HIV/prevenção & controle , Educação em Saúde/organização & administração , Promoção da Saúde/métodos , Autonomia Pessoal , Educação Sexual , Parceiros Sexuais , Adulto , Idoso , Preservativos/estatística & dados numéricos , Feminino , Infecções por HIV/psicologia , Haiti , Conhecimentos, Atitudes e Prática em Saúde , Promoção da Saúde/economia , Humanos , Entrevistas como Assunto , Pobreza , Comportamento de Redução do Risco , Sexo Seguro/estatística & dados numéricos , Comportamento Sexual , Fatores Socioeconômicos , Adulto JovemRESUMO
Argentine ants, Linepithema humile (Mayr) (Hymenoptera: Formicidae), form mutualisms with hemipteran pests in crop systems. In vineyards, they feed on honeydew produced by mealybugs and soft scales, which they tend and protect from natural enemies. Few options for controlling Argentine ants are available; one of the more effective approaches is to use liquid baits containing a low dose of an insecticide. Knowledge of ant foraging patterns is required to estimate how many bait stations to deploy per unit area. To measure how far ants move liquid bait in vineyards, we placed bait stations containing sugar water and a protein marker in plots for 6 d, and then collected ants along transects extending away from bait stations. The ants moved an average of 16.08 m and 12.21 m from bait stations in the first and second years of the study, respectively. Marked ants were found up to 63 m from bait stations; however, proportions of marked ants decreased exponentially as distance from the bait station increased. Results indicate that Argentine ants generally forage at distances <36 m in California vineyards, thus suggesting that insecticide bait stations must be deployed at intervals of 36 m or less to control ants. We found no effect of insecticide on distances that ants moved the liquid bait, but this may have been because bait station densities were too low to affect the high numbers of Argentine ants that were present at the study sites.
Assuntos
Formigas , Comportamento Animal , Controle de Insetos , Vitis , Animais , CaliforniaRESUMO
BACKGROUND AND PURPOSE: Short-term exposure to ambient fine particulate pollution (PM2.5) has been linked to increased stroke. Few studies, however, have examined the effects of long-term exposure. METHODS: A total of 45 625 participants were interviewed and included in this study, the participants came from the Study on Global Ageing and Adult Health, a prospective cohort in 6 low- and middle-income countries. Ambient PM2.5 levels were estimated for participants' communities using satellite data. A multilevel logistic regression model was used to examine the association between long-term PM2.5 exposure and stroke. Potential effect modification by physical activity and consumption of fruit and vegetables was assessed. RESULTS: The odds of stroke were 1.13 (95% confidence interval, 1.04-1.22) for each 10 µg/m3 increase in PM2.5. This effect remained after adjustment for confounding factors including age, sex, smoking, and indoor air pollution (adjusted odds ratio=1.12; 95% confidence interval, 1.04-1.21). Further stratified analyses suggested that participants with higher levels of physical activity had greater odds of stroke, whereas those with higher consumption of fruit and vegetables had lower odds of stroke. These effects remained robust in sensitivity analyses. We further estimated that 6.55% (95% confidence interval, 1.97%-12.01%) of the stroke cases could be attributable to ambient PM2.5 in the study population. CONCLUSIONS: This study suggests that ambient PM2.5 may increase the risk of stroke and may be responsible for the astounding stroke burden in low- and middle-income countries. In addition, greater physical activity may enhance, whereas greater consumption of fruit and vegetables may mitigate the effect.
Assuntos
Poluição do Ar/estatística & dados numéricos , Exposição Ambiental/estatística & dados numéricos , Exercício Físico/fisiologia , Frutas , Material Particulado/efeitos adversos , Acidente Vascular Cerebral/epidemiologia , Acidente Vascular Cerebral/etiologia , Verduras , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Dieta , Feminino , Gana/epidemiologia , Humanos , Índia/epidemiologia , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Estudos Prospectivos , Federação Russa/epidemiologia , África do Sul/epidemiologiaRESUMO
The transcription factor signal STAT5 is constitutively activated in a wide range of leukemias and lymphomas, and drives the expression of genes necessary for proliferation, survival, and self-renewal. Thus, targeting STAT5 is an appealing therapeutic strategy for hematologic malignancies. Given the importance of bromodomain-containing proteins in transcriptional regulation, we considered the hypothesis that a pharmacologic bromodomain inhibitor could inhibit STAT5-dependent gene expression. We found that the small-molecule bromodomain and extra-terminal (BET) bromodomain inhibitor JQ1 decreases STAT5-dependent (but not STAT3-dependent) transcription of both heterologous reporter genes and endogenous STAT5 target genes. JQ1 reduces STAT5 function in leukemia and lymphoma cells with constitutive STAT5 activation, or inducibly activated by cytokine stimulation. Among the BET bromodomain subfamily of proteins, it seems that BRD2 is the critical mediator for STAT5 activity. In experimental models of acute T-cell lymphoblastic leukemias, where activated STAT5 contributes to leukemia cell survival, Brd2 knockdown or JQ1 treatment shows strong synergy with tyrosine kinase inhibitors (TKI) in inducing apoptosis in leukemia cells. In contrast, mononuclear cells isolated form umbilical cord blood, which is enriched in normal hematopoietic precursor cells, were unaffected by these combinations. These findings indicate a unique functional association between BRD2 and STAT5, and suggest that combinations of JQ1 and TKIs may be an important rational strategy for treating leukemias and lymphomas driven by constitutive STAT5 activation.
Assuntos
Neoplasias Hematológicas/genética , Neoplasias Hematológicas/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Proteínas Serina-Treonina Quinases/antagonistas & inibidores , Fator de Transcrição STAT5/metabolismo , Azepinas/farmacologia , Benzodiazepinas/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Técnicas de Silenciamento de Genes , Humanos , Leucemia/genética , Leucemia/metabolismo , Proteínas Serina-Treonina Quinases/genética , RNA Interferente Pequeno/genética , Fatores de Transcrição , Triazóis/farmacologiaRESUMO
Liquid baits were evaluated for control of the Argentine ant, Linepithema humile (Mayr) (Hymenoptera: Formicidae), and associated mealybug and soft scale pests in California vineyards. In 2003, liquid baits with small doses ofimidacloprid, boric acid, or thiamethoxam dissolved in 25% sucrose water resulted in lower ant and mealybug densities and fruit damage, compared with an untreated control. Similar treatments in a soft scale-infested vineyard showed only a reduction of ant density and fruit infestation in only the boric acid and thiamethoxam treatments. In 2004, commercial and noncommercial formulations of liquid baits reduced ant densities in three separate trials, but they had inconsistent effects on mealybug densities and fruit infestation; granular protein bait had no effect. Using large plots and commercial application methodologies, liquid bait deployed in June resulted in lower ant density and fruit infestation, but it had no effect on mealybug density. Across all trials, liquid bait treatments resulted in lower ant density (12 of 14 trials) and fruit damage (11 of 14 sites), presenting the first report of liquid baits applied using commercial methodologies that resulted in a reduction of ants and their associated hemipteran crop damage. For commercialization of liquid baits, we showed that any of the tested insecticides can suppress Argentine ants when properly delivered in the crop system. For imidacloprid, bait dispensers must be protected from sunlight to reduce photodegradation. Results suggest that incomplete ant suppression can suppress mealybug densities. However, after ant populations are suppressed, there may be a longer period before hemipteran populations are effectively suppressed. Therefore, liquid baits should be considered part of a multiseason program rather than a direct, in-season control of hemipteran pest populations.
Assuntos
Formigas , Inseticidas , Vitis/parasitologia , Animais , Argentina , Ácidos Bóricos/farmacologia , Imidazóis , Controle de Insetos/métodos , Neonicotinoides , Nitrocompostos , Oxazinas , Densidade Demográfica , Estações do Ano , Tiametoxam , TiazóisRESUMO
Argentine ants, Linepithema humile (Mayr), have a positive effect on populations of mealybugs (Pseudococcus spp.) in California vineyards. Previous studies have shown reductions in both ant activity and mealybug numbers after liquid ant baits were deployed in vineyards at densities of 85-620 bait stations/ha. However, bait station densities may need to be <85 bait stations/ha before bait-based strategies for ant control are economically comparable to spray-based insecticide treatments-a condition that, if met, will encourage the commercial adoption of liquid baits for ant control. This research assessed the effectiveness of baits deployed at lower densities. Two field experiments were conducted in commercial vineyards. In experiment 1, baits were deployed at 54-225 bait stations/ha in 2005 and 2006. In experiment 2, baits were deployed at 34-205 bait stations/ha in 2006 only. In both experiments, ant activity and the density of mealybugs in grape fruit clusters at harvest time declined with increasing bait station density. In 2005 only, European fruit lecanium scale [Parthenolecanium corni (Bouché)] were also present in fruit clusters, and scale densities were negatively related to bait station density. The results indicate that the amount of ant and mealybug control achieved by an incremental increase in the number of bait stations per hectare is constant across a broad range of bait station densities. The results are discussed in the context of commercializing liquid ant baits to provide a more sustainable Argentine ant control strategy.