RESUMO
Endometrial cancer (EC) is a significant cause of cancer-related deaths in women. MicroRNAs (miRs) play a role in cancer development, acting as oncogenes or tumor suppressors. This study evaluated the diagnostic potential of hsa-miR-185-5p and hsa-miR-191-5p in EC and their correlation with clinical and histopathological features. A cross-sectional study analyzed formalin-fixed, paraffin-embedded tissue samples from 59 patients: 18 with EC, 21 with endometrial hyperplasia (EH), 17 with normal endometrium (NE), and 3 with endometrial polyps (EPs). Quantitative reverse transcription-polymerase chain reaction and TaqMan probes were used for miR expression analysis. The Shapiro-Wilk test was used to analyze the normal distribution of the data. Subsequently, parametric or non-parametric tests were used to evaluate the associations between the expression levels of each miR and clinical parameters. Both miRs were underexpressed in some precursor and malignant lesions compared to certain NE subtypes and benign lesions. Specifically, hsa-miR-185-5p showed underexpression in grade 3 EC compared to some NE and EH subtypes (FC: -57.9 to -8.5, p < 0.05), and hsa-miR-191-5p was underexpressed in EH and EC compared to secretory endometrium and EPs (FC: -4.2 to -32.8, p < 0.05). SETD1B, TJP1, and MSI1 were common predicted target genes. In conclusion, hsa-miR-185-5p and hsa-miR-191-5p are underexpressed in EC tissues, correlating with histopathological grades, highlighting their potential as diagnostic biomarkers and their role as tumor suppressors in EC.
Assuntos
Neoplasias do Endométrio , Endométrio , Regulação Neoplásica da Expressão Gênica , MicroRNAs , Humanos , Feminino , MicroRNAs/genética , MicroRNAs/metabolismo , Neoplasias do Endométrio/genética , Neoplasias do Endométrio/patologia , Neoplasias do Endométrio/metabolismo , Endométrio/metabolismo , Endométrio/patologia , Pessoa de Meia-Idade , Estudos Transversais , Gradação de Tumores , Adulto , Idoso , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismoRESUMO
Un factor de vital importancia para el diseño mecánico de distractor osteogénico alveolar es el conocimiento de las cargas fisiológicas para la simulación computacional, existe muy poca información o trabajos previos sobre este tema. Para obtener estas cargas, ha sido desarrollada una celda de carga constituida de una lámina de acero inoxidable en voladizo instrumentada con galgas extensométricas, la cual ha sido empotrada en una férula como la usada en tratamientos de ortodoncias. Luego de calibrar la celda de cargas fisiológicas, se realizó un ensayo in vivo sobre un paciente parcialmente edéntulo del maxilar inferior, obteniéndose registros digitales de cargas para diferentes condiciones de protrusión de la lengua, retracción del labio inferior y la combinación de ambas.
A key aspect involved in the mechanical design of alveolar distractor is the accurate evaluation of physiological loading for computer simulation. Very few information and previous work are available about this subject. A stainless steel load cell having strain gauges has been developed and manufactured. This cell was attached to a splint, like those used in orthodontia. The load cell was calibrated and then an in vivo test was carried out on a partially-edentulous patient. Digital registers of load were obtained for three cases: tongue protrusion, lower lips retraction and a combination of both.
RESUMO
Introducción: En el presente estudio in vitro se evaluó la permeabilidad de los conductos subobturados con tres cementos selladores, los cuales fueron retratados de manera químico - mecánica con el objetivo de alcanzar la longitud de trabajo óptima. Material y método: Fueron utilizados 60 conductos de las raíces mesiales de molares inferiores permanentes, divididos en tres grupos: (G1) Gutapercha - Endofill, (G2) Gutapercha - Roeko seal y (G3) el sistema de obturación Resilon (conos de resina y Epiphany Seal); los cuales fueron retratados mediante el sistema Pro-taper Re tratamiento (D1, D2) y el cloroformo como disolvente. Resultados: Fueron evaluados cualitativamente empleando la prueba de chi cuadrado, encontrándose que fue estadísticamente significativa (p<0,005) entre los grupos (G1) gutapercha-endofill y (G2) gutapercha Roeko seal en comparación con el (G3) sistema Resilon Epiphany seal. Conclusiones: El estudio demostró que los cementos selladores Endofill y Roeko Seal presentaron mayor factibilidad en la permeabilización que el Epiphany en conductos subobturados.
Introduction: In this in vitro study, there was evaluated the permeability of the underfilling root canals with three sealers, which were portrayed in chemical-mechanical way in order to achieve optimal working length. Material and Methods: There were used 60 mesial root canals of permanent lower molars divided (G1) Gutta-percha - Endofill, (G2) Gutta-percha - Roek Seal and (G3) Resilon obturation system (cones and resin Epiphany Seal), which were portrayed by the Pro-taper Re treatment (D1, D2) and chloroform as solvent. Results: There were evaluated qualitatively using the Chi Square test finding that it was statistically significant (p <0.005) among the groups (G1) and gutta-endofill (G2) Roek percha seal compared to the (G3) Epiphany Resilon system seal. Conclusions: The study showed that sealers: Endofill and Roeko seal showed higher permeability than the Epiphany in under filling root canals.