Assuntos
Anticorpos Monoclonais Murinos/administração & dosagem , Antineoplásicos/administração & dosagem , Inibidores dos Fatores de Coagulação Sanguínea/sangue , Fator V , Hemorragia/sangue , Hemorragia/tratamento farmacológico , Idoso , Animais , Bovinos , Feminino , Seguimentos , Hemorragia/induzido quimicamente , Hemostáticos/administração & dosagem , Hemostáticos/efeitos adversos , Humanos , Rituximab , Trombina/administração & dosagem , Trombina/efeitos adversosRESUMO
OBJECTIVES: Fibroadenoma is the most common benign mammary condition among women aged 35 or younger. Expression of Ki-67 antigen has been used to compare proliferative activity of mammary fibroadenoma epithelium in the follicular and luteal phases of the menstrual cycle. MATERIALS AND METHODS: Ninety eumenorrheic women were selected for tumour excision; they were assigned to either of the two groups, according to their phase of menstrual cycle. At the end of the study, 75 patients with 87 masses were evaluated by epithelial cell Ki-67 expression, blind (no information given concerning group to which any lesion belonged). RESULTS: Both groups were found to be homogeneous relative to age, menarche, body mass index, previous gestation, parity, breastfeeding, number of fibroadenomas, family history of breast cancer and tabagism. Median tumour size was 2.0 cm and no relationship between proliferative activity and nodule diameter was observed. No typical pattern was observed in the expression of Ki-67 in distinct nodules of the same patient. Average values for expression of Ki-67 (per 1000 epithelial cells) in follicular and luteal phases were 27.88 and 37.88, respectively (P = 0.116). CONCLUSION: Our findings revealed that proliferative activities in the mammary fibroadenoma epithelium did not present a statistically significant difference in the follicular and luteal phases. The present study contributes to clarifying that fibroadenoma is a neoplasm and does not undergo any change in the proliferative activity during the menstrual cycle.
Assuntos
Neoplasias da Mama/patologia , Proliferação de Células , Fibroadenoma/patologia , Fase Folicular/metabolismo , Antígeno Ki-67/metabolismo , Fase Luteal/metabolismo , Adolescente , Adulto , Fatores Etários , Aleitamento Materno/estatística & dados numéricos , Neoplasias da Mama/complicações , Neoplasias da Mama/metabolismo , Feminino , Fibroadenoma/complicações , Fibroadenoma/metabolismo , Fase Folicular/sangue , Humanos , Fase Luteal/sangue , Obesidade/complicações , Progesterona/sangue , Tabagismo/complicações , Adulto JovemRESUMO
We determined the nuclear volume of fibroblasts of the normal mammary lobular stroma during the follicular and luteal phases of the menstrual cycle. Twenty patients aged 15 to 35 years and eumenorrheic for at least 6 months were randomly assigned to 2 groups, i.e., 10 women in the follicular phase and 10 in the luteal phase. The nuclear volume was 34.4 micron 3 and 98.8 micron 3 for the follicular and luteal phases, respectively, with the difference being statistically significant (p < 0.05). These data suggest a higher metabolic activity in the mammary intralobular stroma during the luteal phase of the menstrual cycle, probably due to a synergistic action of estradiol and progesterone.
Assuntos
Mama/citologia , Fibroblastos/citologia , Fase Folicular , Fase Luteal , Adolescente , Adulto , Feminino , Humanos , Distribuição AleatóriaRESUMO
The immune response of sheep to somatic components and excretory/secretory products of adult Fasciola hepatica, was studied during an experimental infection. Antibodies against adult fluke somatic and excretory/secretory antigens were detected by thin layer immunoassay from the second week post infection. Similarly, the results of Western blot analysis showed specific recognition of several components as early as two weeks after infection. However, an increase in the number and intensity of bands with time of infection was observed in the patterns of antigenic recognition. Most notorious was the strong reactivity of all infected sheep sera towards components of 20-23 kDa in the somatic preparation and components of 23-27 kDa in the excretory/secretory products of adult F. hepatica, specially noticeable after the sixth week post-infection. Since these polypeptides were recognized by all infected animals, they could play an important role in the diagnosis of sheep fascioliasis.
Assuntos
Antígenos de Helmintos/isolamento & purificação , Fasciola hepatica/imunologia , Fasciolíase/veterinária , Doenças dos Ovinos/imunologia , Animais , Anticorpos Anti-Helmínticos/imunologia , Antígenos de Helmintos/classificação , Antígenos de Helmintos/imunologia , Western Blotting , Eletroforese em Gel de Poliacrilamida , Fasciolíase/imunologia , Ovinos/imunologiaRESUMO
The histopathology of the skin basal cell carcinomas (BCCs) with areas of intermediate differentiation (ID) has been investigated. In a series of 127 BCCs, areas of ID were found in 28 tumors (22%), and also in an additional 10 cases of other series. These areas consisted of compact masses of cells without peripheral palisading, and with intermediate differentiation between basal and squamous cells. In comparison with the common undifferentiated BCCs, the BCCs with the ID areas may behave in a more aggressive fashion, since they displayed more precocious ulceration in small tumors (p less than 0.001), greater infiltrative features (p less than 0.001), more atypical cells (p less than 0.001) with increased nuclear-cytoplasmic ratio and more mitoses (p less than 0.001). The relation of such basal cell carcinomas to the metatypical carcinoma of the skin was discussed. Metatypical carcinoma, however, has been poorly defined and thus has no general acceptance in the literature. The new definition of the basosquamous cell carcinoma and the presence of intermediate areas of differentiation in this tumor were emphasized, and it was suggested that metastatic basal cell carcinoma and metatypical carcinoma may be the same tumor.