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J Infect Dis ; 222(4): 590-600, 2020 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-32193549

RESUMO

BACKGROUND: The 4 antigenically distinct serotypes of dengue virus (DENV) share extensive homology with each other and with the closely related Zika flavivirus (ZIKV). The development of polyclonal memory B cells (MBCs) to the 4 DENV serotypes and ZIKV during DENV infection is not fully understood. METHODS: In this study, we analyzed polyclonal MBCs at the single-cell level from peripheral blood mononuclear cells collected ~2 weeks or 6-7 months postprimary or postsecondary DENV infection from a pediatric hospital-based study in Nicaragua using a Multi-Color FluoroSpot assay. RESULTS: Dengue virus elicits robust type-specific and cross-reactive MBC responses after primary and secondary DENV infection, with a significantly higher cross-reactive response in both. Reactivity to the infecting serotype dominated the total MBC response. Although the frequency and proportion of type-specific and cross-reactive MBCs were comparable between primary and secondary DENV infections, within the cross-reactive response, the breadth of MBC responses against different serotypes was greater after secondary DENV infection. Dengue virus infection also induced cross-reactive MBC responses recognizing ZIKV, particularly after secondary DENV infection. CONCLUSIONS: Overall, our study sheds light on the polyclonal MBC response to DENV and ZIKV in naive and DENV-preimmune subjects, with important implications for natural infections and vaccine development.


Assuntos
Linfócitos B/imunologia , Linfócitos B/metabolismo , Reações Cruzadas/imunologia , Interações Hospedeiro-Patógeno/imunologia , Memória Imunológica , Adolescente , Anticorpos Antivirais/imunologia , Criança , Pré-Escolar , Dengue/imunologia , Dengue/virologia , Vírus da Dengue/imunologia , ELISPOT , Feminino , Hospitais Pediátricos , Humanos , Lactente , Leucócitos Mononucleares , Masculino , Nicarágua , Sorogrupo , Zika virus/imunologia , Infecção por Zika virus/imunologia , Infecção por Zika virus/virologia
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