RESUMO
Staphylococcus aureus es un patógeno responsable de diversos cuadros clínicos. Los marcadores moleculares son útiles para el estudio de la epidemiología microbiana. Se estudiaron 22 aislamientos de S. aureus resistentes a meticilina (SARM) y 23 sensibles a meticilina (SASM) mediante mecA, cassette SCCmec, leucocidina de Panton Valentine (LPV) y polimorfismo spa; se analizaron datos de los pacientes. SASM predominó en muestras distintas de piel y partes blandas de internados, mientras SARM en partes blandas. Predominó el SCCmec tipo IV seguido del I. Se encontró baja presencia de LPV. En SARM hubo 11 tipos de spa diferentes, t019 fue el más frecuente y en pacientes ambulatorios. En SASM se hallaron 17 tipos con prevalencia del t189. El spa t002 estuvo presente en SASM y SARM. Se hallaron 11 tipos de spa no reportados en nuestro país.
Staphylococcus aureus is a pathogen associated a different kind of infection. Molecular markers are useful tools to study microbial epidemiology. Twenty two methicillin-resistant S. aureus (MRSA) and 23 methicillin-susceptible S. aureus (MSSA) were studied by mecA gene, SCCmec cassette, Panton Valentine leucocidin (PVL) and spa polymorphism. The clinical data patients were analyzed. MSSA was prevalent in samples different from skin and soft tissue (SST) and in hospitalized patients, whereas MRSA in SST. SCCmec type IV was predominant, followed spa; by type I. Low presence of PVL was found. In MRSA 11 different types of spa were detected, SCCmec; t019 was the most frequent and associated with outpatient, 17 types were found in MSSA and Panton Valentine t189 was prevalent. spa t002 was present in MSSA and MRSA. We found 11 types of spa not leucocidin reported in our country.
Assuntos
Adulto , Humanos , Infecções Estafilocócicas , Staphylococcus aureus , Staphylococcus aureus Resistente à Meticilina , Hospitais , Argentina , Proteínas de Bactérias , Toxinas Bacterianas , Testes de Sensibilidade Microbiana , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Staphylococcus aureus Resistente à Meticilina/genética , AntibacterianosRESUMO
Staphylococcus aureus is a pathogen associated a different kind of infection. Molecular markers are useful tools to study microbial epidemiology. Twenty two methicillin-resistant S. aureus (MRSA) and 23 methicillin-susceptible S. aureus (MSSA) were studied by mecA gene, SCCmec cassette, Panton Valentine leucocidin (PVL) and spa polymorphism. The clinical data patients were analyzed. MSSA was prevalent in samples different from skin and soft tissue (SST) and in hospitalized patients, whereas MRSA in SST. SCCmec type IV was predominant, followed by type I. Low presence of PVL was found. In MRSA 11 different types of spa were detected, t019 was the most frequent and associated with outpatient, 17 types were found in MSSA and t189 was prevalent. spa t002 was present in MSSA and MRSA. We found 11 types of spa not reported in our country.
Assuntos
Hospitais , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Staphylococcus aureus , Adulto , Antibacterianos , Argentina , Proteínas de Bactérias , Toxinas Bacterianas , Humanos , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Testes de Sensibilidade MicrobianaRESUMO
We herein present the case of an adult male patient who consulted for lower extremity edema, a 2- month history of fever and oppressive chest pain radiating to the left arm. He referred neither contact with breeding animals nor consumption of unpasteurized dairy products. A diagnosis of endocarditis was confirmed by cardiac studies. Since the empirical treatment with cephalotin, ampicillin and gentamicin failed, the patient underwent aortic valve replacement. A total of four blood cultures were positive with a gram-negative rod. Bacterial identification was performed using the API 20 NE technique (bioMèrieux), the Phoenix automated method (BD) and conventional biochemical tests which were unable to classify the isolate as to genus and species. The strain was sent to the INEI-ANLIS "Dr. Carlos G. Malbrán" where it was identified as Brucella canis. The antimicrobial treatment was switched to doxycycline, rifampicin and trimethoprim-sulfamethoxazole with good evolution of the patient. The clinical significance of this case report lies in the possible failure of the empiric antibiotic therapy administered for endocarditis, since B. canis did not respond to the conventional antimicrobial treatment for this pathology.
Assuntos
Brucella canis/isolamento & purificação , Brucelose/microbiologia , Endocardite Bacteriana/microbiologia , Adulto , Valva Aórtica/microbiologia , Valva Aórtica/cirurgia , Argentina/epidemiologia , Bacteriemia/microbiologia , Técnicas de Tipagem Bacteriana , Brucella canis/efeitos dos fármacos , Brucelose/tratamento farmacológico , Brucelose/epidemiologia , Brucelose/cirurgia , Dor no Peito/etiologia , Terapia Combinada , Doxiciclina/uso terapêutico , Farmacorresistência Bacteriana Múltipla , Edema/etiologia , Endocardite Bacteriana/tratamento farmacológico , Endocardite Bacteriana/epidemiologia , Endocardite Bacteriana/cirurgia , Febre/etiologia , Implante de Prótese de Valva Cardíaca , Humanos , Masculino , Rifampina/uso terapêutico , Combinação Trimetoprima e Sulfametoxazol/uso terapêuticoRESUMO
We herein present the case of an adult male patient who consulted for lower extremity edema, a 2- month history of fever and oppressive chest pain radiating to the left arm. He referred neither contact with breeding animals nor consumption of unpasteurized dairy products. A diagnosis of endocarditis was confirmed by cardiac studies. Since the empirical treatment with cephalotin, ampicillin and gentamicin failed, the patient underwent aortic valve replacement. A total of four blood cultures were positive with a gram-negative rod. Bacterial identification was performed using the API 20 NE technique (bioMÞrieux), the Phoenix automated method (BD) and conventional biochemical tests which were unable to classify the isolate as to genus and species. The strain was sent to the INEI-ANLIS "Dr. Carlos G. Malbrán" where it was identified as Brucella canis. The antimicrobial treatment was switched to doxycycline, rifampicin and trimethoprim-sulfamethoxazole with good evolution of the patient. The clinical significance of this case report lies in the possible failure of the empiric antibiotic therapy administered for endocarditis, since B. canis did not respond to the conventional antimicrobial treatment for this pathology.
Assuntos
Brucella canis/isolamento & purificação , Brucelose/microbiologia , Endocardite Bacteriana/microbiologia , Adulto , Argentina/epidemiologia , Bacteriemia/microbiologia , Brucella canis/efeitos dos fármacos , Brucelose/cirurgia , Brucelose/epidemiologia , Brucelose/tratamento farmacológico , Combinação Trimetoprima e Sulfametoxazol/uso terapêutico , Dor no Peito/etiologia , Doxiciclina/uso terapêutico , Edema/etiologia , Endocardite Bacteriana/cirurgia , Endocardite Bacteriana/epidemiologia , Endocardite Bacteriana/tratamento farmacológico , Farmacorresistência Bacteriana Múltipla , Febre/etiologia , Humanos , Implante de Prótese de Valva Cardíaca , Masculino , Rifampina/uso terapêutico , Terapia Combinada , Técnicas de Tipagem Bacteriana , Valva Aórtica/cirurgia , Valva Aórtica/microbiologiaRESUMO
We herein present the case of an adult male patient who consulted for lower extremity edema, a 2- month history of fever and oppressive chest pain radiating to the left arm. He referred neither contact with breeding animals nor consumption of unpasteurized dairy products. A diagnosis of endocarditis was confirmed by cardiac studies. Since the empirical treatment with cephalotin, ampicillin and gentamicin failed, the patient underwent aortic valve replacement. A total of four blood cultures were positive with a gram-negative rod. Bacterial identification was performed using the API 20 NE technique (bioMÞrieux), the Phoenix automated method (BD) and conventional biochemical tests which were unable to classify the isolate as to genus and species. The strain was sent to the INEI-ANLIS "Dr. Carlos G. Malbrán" where it was identified as Brucella canis. The antimicrobial treatment was switched to doxycycline, rifampicin and trimethoprim-sulfamethoxazole with good evolution of the patient. The clinical significance of this case report lies in the possible failure of the empiric antibiotic therapy administered for endocarditis, since B. canis did not respond to the conventional antimicrobial treatment for this pathology.
Assuntos
Brucella canis/isolamento & purificação , Brucelose/microbiologia , Endocardite Bacteriana/microbiologia , Adulto , Valva Aórtica/microbiologia , Valva Aórtica/cirurgia , Argentina/epidemiologia , Bacteriemia/microbiologia , Técnicas de Tipagem Bacteriana , Brucella canis/efeitos dos fármacos , Brucelose/tratamento farmacológico , Brucelose/epidemiologia , Brucelose/cirurgia , Dor no Peito/etiologia , Terapia Combinada , Doxiciclina/uso terapêutico , Farmacorresistência Bacteriana Múltipla , Edema/etiologia , Endocardite Bacteriana/tratamento farmacológico , Endocardite Bacteriana/epidemiologia , Endocardite Bacteriana/cirurgia , Febre/etiologia , Implante de Prótese de Valva Cardíaca , Humanos , Masculino , Rifampina/uso terapêutico , Combinação Trimetoprima e Sulfametoxazol/uso terapêuticoRESUMO
The early detection of anti-CagA antibodies in young adults would be of a great clinical impact in the prevention of gastric cancer. The aim of this study was to assess IgG and anti-CagA antibodies seroprevalence against Helicobacter pylori in our region using a non invasive technique, easy to perform an evaluating its relationship with different epidemiological risk factors. Four hundred and thirty-five volunteers with a mean age of forty years old from different health centers were included in this study. Demographic socieconomical and other data of interest were recorded. IgG and CagA antibodies against Helicobacter pylori were determined using an enzyme immunoassay technique. The prevalence of IgG antibodies was 52.2% being 152 in women (53.7%) and 75 in men (49%). These antibodies were showed in 65% of affected patients and 43.1% in asymptomatic carriers. The prevalence anti-CagA antibodies was 63.4% among seropositives (IgG) individuals corresponding to 33.1% of the total studied population. Ninety-six (33.9%) persons of this group were women an 48 (31.6%) were men. The prevalence was 45.4% and 25.7% in symptomatic an asymptomatic individuals, respectively. It was demonstrated that IgG antibodies were associated with age, area of residence, educational level reached and number of bedrooms in the house. The anti-CagA antibodies depend on area of residence and symptoms. The relation between the symptoms and the presence of anti-CagA antibodies shows the selective importance of clinical data in the gastric diseases associated with Helicobacter pylori.
Assuntos
Anticorpos Anti-Idiotípicos/sangue , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/sangue , Proteínas de Bactérias/sangue , Infecções por Helicobacter/epidemiologia , Helicobacter pylori/imunologia , Adolescente , Adulto , Idoso , Anticorpos Anti-Idiotípicos/isolamento & purificação , Argentina/epidemiologia , Feminino , Infecções por Helicobacter/sangue , Humanos , Imunoglobulina G/sangue , Imunoglobulina G/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Fatores de Risco , Estudos SoroepidemiológicosRESUMO
The early detection of anti-CagA antibodies in young adults would be of a great clinical impact in the prevention of gastric cancer. The aim of this study was to assess IgG and anti-CagA antibodies seroprevalence against Helicobacter pylori in our region using a non invasive technique, easy to perform an evaluating its relationship with different epidemiological risk factors. Four hundred and thirty-five volunteers with a mean age of forty years old from different health centers were included in this study. Demographic socieconomical and other data of interest were recorded. IgG and CagA antibodies against Helicobacter pylori were determined using an enzyme immunoassay technique. The prevalence of IgG antibodies was 52.2
being 152 in women (53.7
) and 75 in men (49
). These antibodies were showed in 65
of affected patients and 43.1
in asymptomatic carriers. The prevalence anti-CagA antibodies was 63.4
among seropositives (IgG) individuals corresponding to 33.1
of the total studied population. Ninety-six (33.9
) persons of this group were women an 48 (31.6
) were men. The prevalence was 45.4
and 25.7
in symptomatic an asymptomatic individuals, respectively. It was demonstrated that IgG antibodies were associated with age, area of residence, educational level reached and number of bedrooms in the house. The anti-CagA antibodies depend on area of residence and symptoms. The relation between the symptoms and the presence of anti-CagA antibodies shows the selective importance of clinical data in the gastric diseases associated with Helicobacter pylori.