RESUMO
Crohn's disease (CD) and rheumatoid arthritis (RA) are immune mediated inflammatory diseases. Several studies indicate a role for microRNAs (miRNAs) in the pathogenesis of a variety of autoimmune diseases, including CD and RA. Our study's goal was to investigate circulating miRNAs in CD and RA patients to identify potential new biomarkers for early detection and personalized therapeutic approaches for autoimmune diseases. For this study, subjects with CD (n = 7), RA (n = 8) and healthy controls (n = 7) were recruited, and plasma was collected for miRNA sequencing. Comparison of the expression patterns of miRNAs between CD and healthy patients identified 99 differentially expressed miRNAs. Out of these miRNAs, 4 were down regulated, while 95 were up regulated. Comparison of miRNAs between RA and healthy patients identified 57 differentially expressed miRNAs. Out of those, 12 were down regulated, while 45 were up regulated. For all the miRNAs down regulated in CD and RA patients, 420 GO terms for biological processes were similarly regulated between both groups. Therefore, the identification of new plasma miRNAs allows the emergence of new biomarkers that can assist in the diagnosis and treatment of CD and RA.
RESUMO
Reduced inflammation, increased insulin sensitivity, and protection against cancer are shared between humans and mice with GH/IGF1 deficiency. Beyond hormone levels, miRNAs are important regulators of metabolic changes associated with healthy aging. We hypothesized that GH deficiency in humans alters the abundance of circulating miRNAs and that a subset of those miRNAs may overlap with those found in GH-deficient mice. In this study, subjects with untreated congenital isolated GH deficiency (IGHD; n = 23) and control subjects matched by age and sex (n = 23) were recruited and serum was collected for miRNA sequencing. Serum miRNAs from young (6 month) and old (22 month) Ames dwarf (df/df) mice with GH deficiency and their WT littermates (n = 5/age/genotype group) were used for comparison. We observed 14 miRNAs regulated with a genotype by age effect and 19 miRNAs regulated with a genotype effect independent of age in serum of IGHD subjects. These regulated miRNAs are known for targeting pathways associated with longevity such as mTOR, insulin signaling, and FoxO. The aging function was overrepresented in IGHD individuals, mediated by hsa-miR-31, hsa-miR-146b, hsa-miR-30e, hsa-miR-100, hsa-miR-181b-2, hsa-miR-195, and hsa-miR-181b-1, which target the FoxO and mTOR pathways. Intriguingly, miR-181b-5p, miR-361-3p, miR-144-3p, and miR-155-5p were commonly regulated in the serum of humans and GH-deficient mice. In vitro assays confirmed target genes for the main up-regulated miRNAs, suggesting miRNAs regulated in IGHD individuals can regulate the expression of age-related genes. These findings indicate that systemic miRNAs regulated in IGHD individuals target pathways involved in aging in both humans and mice.
Assuntos
Nanismo Hipofisário/genética , MicroRNAs/genética , Adulto , Animais , Feminino , Humanos , Masculino , Camundongos , Pessoa de Meia-IdadeRESUMO
Empleando ENZYMEBA, procedimiento diagnóstico de amebiasis intestinal desarrollado en el Instituto de Medicina Tropical "Pedro Kourí", se demostró que en la provincia Cienfuegos el examen microscópico de heces se asocia con un marcado sobrediagnóstico de esta parasitosis (de 424 muestras positivas a Entamoeba histolytica, por examen microscópico, solo 61 lo fueron por ENZYMEBA). Para incursionar en los factores que podrían estar incidiendo en la calidad del trabajo de los técnicos de laboratorio de esta provincia se aplicó a la casi totalidad de ellos ( 96 de 102) un cuestionario (23 preguntas) sobre diversos aspectos relacionados con este tipo de diagnóstico. El conjunto de los resultados de esta encuesta sugiere que en la calidad del diagnóstico de amebiasis intestinal por examen microscópico podría estar incidiendo, además de deficiencias técnico-organizativas y materiales, una insuficiente preparación teórica de quienes la realizan
Assuntos
Humanos , Pré-Escolar , Criança , Recém-Nascido , Lactente , Coleta de Dados , Entamoeba histolytica , Fezes , Pessoal de LaboratórioRESUMO
Empleando ENZYMEBA, procedimiento diagnóstico de amebiasis intestinal desarrollado en el Instituto de Medicina Tropical ®Pedro Kourí¼, se demostró que en la provincia Cienfuegos el examen microscópico de heces se asocia a un marcado sobrediagnóstico de esta parasitosis (de 424 muestras positivas a Entamoeba histolytica por examen microscópico sólo 61 lo fueron por ENZYMEBA). Para incusionar en los factores que podrían estar incidiendo en la calidad del trabajo de los técnicos de aquella provincia, aplicamos una encuesta al universo de los responsables de laboratorios de ésta. El conjunto de los resultados de la aplicación de este cuestionario (11 preguntas) nos sugiere que en la calidad del diagnóstico de amebiasis intestinal por examen microscópico de heces podrían estar incidiendo deficiencias técnico-organizativas asociadas a otros factores objetivos y subjetivos
Assuntos
Humanos , Técnicas de Laboratório Clínico/normas , Disenteria Amebiana/diagnóstico , Fezes/parasitologia , Pessoal de Laboratório , Parasitologia , Controle de Qualidade , Inquéritos e QuestionáriosRESUMO
Se demostró, en un estudio previo realizado en la provincia de Cienfuegos, en el que se empleó ENZYMEBA, que en aquella provincia, el examen microscópico de heces para el diagnóstico de amebiasis intestinal se asociaba a falsos diagnósticos. Para conocer de los posibles factores asociados a este problema, se aplicó una encuesta a médicos de la provincia de Cienfuegos, relacionada con el diagnóstico y tratamiento de la amebiasis intestinal. Según el análisis de los resultados, las consecuencias adversas del sobrediagnóstico de amebiasis intestinal pudieran estar amplificadas por la ausencia de percepción del problema, y por el marcado desconocimiento que sobre importantes aspectos de esta parasitosis poseen