RESUMO

Abstract Reports from popular medicine usually act as a basis for the development of new drugs from natural compounds with therapeutic actions for serious diseases and prevalence such as cancer. Bromelia antiacantha Bertol. is a species of the Bromeliaceae family, considered an unconventional food plant, found in the south and midwest regions of Brazil. Despite the high nutritional content and pharmacological potential of its fruits, few scientific studies report its biological actions. Thus, this study evaluates the phytochemical profile of aqueous and ethanol extracts obtained from B. antiacantha fruits, as well as their possible antioxidant, antitumor, and cytotoxic activities. The aqueous extract exhibited phenolic compounds and flavonoids, while ethanol extracts indicated the presence of flavonoids and coumarin in their composition, regardless of the region of collection. The ethanolic extract demonstrated a more promising antioxidant effect than the aqueous extract and also induced a significant inhibition in the viability of human cervical cancer cells of the SiHa strain. In addition, treatment with both extracts did not alter the viability of non-tumor cells of the immortalized human keratinocyte lineage (HaCaT). These results bring new data about extracts obtained from a native plant, edible and traditionally used in popular medicine, opening new perspectives for its possible therapeutic application.

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Resumo Relatos da medicina popular costumam atuar como referencial para o desenvolvimento de novos fármacos a partir de moléculas naturais com ações terapêuticas para doenças de alta gravidade e prevalência como o câncer. Bromelia antiacantha Bertol. é uma espécie da família Bromeliaceae, considerada uma planta alimentícia não convencional (PANC), encontrada nas regiões sul e centro-oeste do Brasil. Apesar do alto teor nutritivo e potencial farmacológico de seus frutos, poucos estudos científicos relatam suas ações biológicas. Desta forma, este estudo avalia o perfil fitoquímico de extratos aquoso e etanólico obtidos de frutos de B. antiacantha, bem como a sua possível ação antioxidante, antitumoral e citotóxica. O extrato aquoso apresentou compostos fenólicos e flavonoides, enquanto os extratos etanólicos apontam a presença de flavonóides e cumarina em sua composição, independente da região de coleta. O extrato etanólico demonstrou efeito antioxidante mais promissor do que o extrato aquoso e também induziu uma inibição significativa na viabilidade de células humanas de câncer cervical da linhagem SiHa. Além disso, o tratamento com ambos extratos não alterou a viabilidade de células não tumorais da linhagem de queratinócitos humanos imortalizados (HaCaT). Estes dados trazem novas informações sobre extratos obtidos de uma espécie vegetal nativa, comestível e já utilizada tradicionalmente, mas abrindo novas perspectivas quanto a possíveis aplicações terapêuticas.

RESUMO

Reports from popular medicine usually act as a basis for the development of new drugs from natural compounds with therapeutic actions for serious diseases and prevalence such as cancer. Bromelia antiacantha Bertol. is a species of the Bromeliaceae family, considered an unconventional food plant, found in the south and midwest regions of Brazil. Despite the high nutritional content and pharmacological potential of its fruits, few scientific studies report its biological actions. Thus, this study evaluates the phytochemical profile of aqueous and ethanol extracts obtained from B. antiacantha fruits, as well as their possible antioxidant, antitumor, and cytotoxic activities. The aqueous extract exhibited phenolic compounds and flavonoids, while ethanol extracts indicated the presence of flavonoids and coumarin in their composition, regardless of the region of collection. The ethanolic extract demonstrated a more promising antioxidant effect than the aqueous extract and also induced a significant inhibition in the viability of human cervical cancer cells of the SiHa strain. In addition, treatment with both extracts did not alter the viability of non-tumor cells of the immortalized human keratinocyte lineage (HaCaT). These results bring new data about extracts obtained from a native plant, edible and traditionally used in popular medicine, opening new perspectives for its possible therapeutic application.

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Relatos da medicina popular costumam atuar como referencial para o desenvolvimento de novos fármacos a partir de moléculas naturais com ações terapêuticas para doenças de alta gravidade e prevalência como o câncer. Bromelia antiacantha Bertol. é uma espécie da família Bromeliaceae, considerada uma planta alimentícia não convencional (PANC), encontrada nas regiões sul e centro-oeste do Brasil. Apesar do alto teor nutritivo e potencial farmacológico de seus frutos, poucos estudos científicos relatam suas ações biológicas. Desta forma, este estudo avalia o perfil fitoquímico de extratos aquoso e etanólico obtidos de frutos de B. antiacantha, bem como a sua possível ação antioxidante, antitumoral e citotóxica. O extrato aquoso apresentou compostos fenólicos e flavonoides, enquanto os extratos etanólicos apontam a presença de flavonóides e cumarina em sua composição, independente da região de coleta. O extrato etanólico demonstrou efeito antioxidante mais promissor do que o extrato aquoso e também induziu uma inibição significativa na viabilidade de células humanas de câncer cervical da linhagem SiHa. Além disso, o tratamento com ambos extratos não alterou a viabilidade de células não tumorais da linhagem de queratinócitos humanos imortalizados (HaCaT). Estes dados trazem novas informações sobre extratos obtidos de uma espécie vegetal nativa, comestível e já utilizada tradicionalmente, mas abrindo novas perspectivas quanto a possíveis aplicações terapêuticas.

Assuntos

Flavonoides , Neoplasias do Colo do Útero , Bromeliaceae , Bromelia , Usos Terapêuticos , Compostos Fitoquímicos , Fitoterapia

RESUMO

Using DFT computational methods, single-walled carbon nanotubes (CNT) are explored in different geometric configurations (armchair, chiral and zigzag) doped with Fe. Geometry, electronic structure and magnetic properties are investigated for all systems, in order to evaluate a potential application of these structures as electrocatalysts in efficient and low-cost fuel cells. In search for a better electrode material, we turn our attention on nature for help. Oxygen molecules are well-known to reveal a remarkable affinity to the heme group. Therefore, we model the adsorption/dissociative behavior of oxygen molecules on carbon nanotubes doped with Fe atoms. We analyze in detail the effect of the chiral nature of carbon nanotubes that governs their electric, magnetic and chemical behavior. Our results indicate that the dissociation phenomenon involving the armchair (5,5) Fe@CNT is more favored than other chiralities and other doped CNT systems, leading to the lowest activation barrier.

RESUMO

Reports from popular medicine usually act as a basis for the development of new drugs from natural compounds with therapeutic actions for serious diseases and prevalence such as cancer. Bromelia antiacantha Bertol. is a species of the Bromeliaceae family, considered an unconventional food plant, found in the south and midwest regions of Brazil. Despite the high nutritional content and pharmacological potential of its fruits, few scientific studies report its biological actions. Thus, this study evaluates the phytochemical profile of aqueous and ethanol extracts obtained from B. antiacantha fruits, as well as their possible antioxidant, antitumor, and cytotoxic activities. The aqueous extract exhibited phenolic compounds and flavonoids, while ethanol extracts indicated the presence of flavonoids and coumarin in their composition, regardless of the region of collection. The ethanolic extract demonstrated a more promising antioxidant effect than the aqueous extract and also induced a significant inhibition in the viability of human cervical cancer cells of the SiHa strain. In addition, treatment with both extracts did not alter the viability of non-tumor cells of the immortalized human keratinocyte lineage (HaCaT). These results bring new data about extracts obtained from a native plant, edible and traditionally used in popular medicine, opening new perspectives for its possible therapeutic application.

Assuntos

Bromelia , Antioxidantes/química , Antioxidantes/farmacologia , Bromelia/química , Flavonoides , Humanos , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia

RESUMO

Mechanical properties of nanomaterials, such as nanowires and nanotubes, are an important feature for the design of novel electromechanical nano-architectures. Since grain boundary structures and surface modifications can be used as a route to modify nanostructured materials, it is of interest to understand how they affect material strength and plasticity. We report large-scale atomistic simulations to determine the mechanical response of nickel nanowires and nanotubes subject to uniaxial compression. Our results suggest that the incorporation of nanocrystalline structure allows completely flexible deformation, in sharp contrast with single crystals. While crystalline structures at high compression are dominated by dislocation pinning and the multiplication of highly localized shear regions, in nanocrystalline systems the dislocation distribution is significantly more homogeneous. Therefore, for large compressions (large strains) coiling instead of bulging is the dominant deformation mode. Additionally, it is observed that nanotubes with only 70% of the nanowire mass but of the same diameter, exhibit similar mechanical behavior up to 0.3 strain. Our results are useful for the design of new flexible and light-weight metamaterials, when highly deformable struts are required.

RESUMO

The use of chitosan to harvest microalgae is a strategic step that seeks to reach an economically competitive price to recover lipids, proteins, and pigments. The aim of the present work was to design low-molecular-weight chitosan from shrimp shells and its physicochemical characterization, to be used for the harvesting of wild microalgae consortia. The chitosan was obtained by chemical deacetylation of shrimp shells, and physicochemical characterization was made using the instrumental methods DSC, TGA, X-ray, FTIR, and SEM. The harvesting of wild microalgae consortia was performed by the jar test method. The obtained chitosan had a low molecular weight (169 KDa), a deacetylation degree of 83 %, a decomposition temperature (TD) of 280 °C, and a crystallinity of 38.2 %. The microalgae genera found in the consortium were Scenedesmus sp., Chlorella sp., Schroderia sp., and Chlamydomonas sp. The microalgae removal efficiency of the chitosan was 99.2 % with 20 mg L-1.

RESUMO

Resumen: Se realizó una simulación in situ del proceso de atención de paciente con diagnóstico de COVID-19 programado para una cirugía de urgencia. Se observó desde la llegada al Departamento de Urgencias y hasta su salida del quirófano. Se llevaron a cabo los protocolos previamente establecidos y se observó por áreas de oportunidad.

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Abstract: An in situ simulation was performed of a patient with COVID-19 diagnosis that is schedule for emergency surgery. From the arrival to the Emergency Department to the exit from the operating theatre. Previously stablished protocols were performed, and areas of improvement were looked for.

RESUMO

nuclear permiten visualizar la estructura y función de un órgano, tejido, hueso o sistema dentro del cuerpo, entre ellos la glándula tiroidea, la cual puede presentar un nódulo tiroideo y este es importante diagnosticar por su potencial malignidad. Objetivo: Determinar las características funcionales de los nódulos tiroideos en 183 pacientes que asistieron al Servicio de Medicina Nuclear del Instituto de Investigaciones en Ciencias de la Salud - UNA durante el período de noviembre del 2016 - noviembre del 2019. Metodología: Fue utilizada la gammacámara SPECT doble cabezal, siguiendo protocolo estandarizado, con administración de pertecnetato de sodio. Resultados: Se observó que la gran mayoría de los pacientes corresponden al sexo femenino 89%, así como que el 64% provienen de la capital y del departamento central; las patologías detectadas corresponden a bocio en 82%, 15% a nódulos (hipercaptantes, hipocaptantes y autónomos) y casos particulares de tiroiditis, tiroides ectópica y adenoma tóxico. La medicina nuclear está directamente involucrada tanto en el diagnóstico como en el tratamiento de la enfermedad tiroidea, por lo que se requiere una comprensión de la fisiopatología y el manejo de los trastornos de la tiroides, de manera a que las políticas de salud pública sean implementadas para el fortalecimiento de la lucha contra estas enfermedades. Conclusión: El trabajo de investigación realizado comprobó que un mayoritario porcentaje de participantes de sexo femenino (89%), con enfermedades tiroideas asistieron al IICS/UNA, y que la frecuencia de bocio entre la totalidad de pacientes que participaron de este estudio fué de 151/183 (82%), los pacientes provenían en su mayoría de hospitales de referencia del departamento Central y coinciden con la localización geográfica de los domicilios de los pacientes, quienes provenían principalmente del mismo departamento Central 118/183 (64%), no obstante también participaron del trabajo de investigación pacientes provenientes de otros departamentos del país. Palabras claves: Diagnóstico, medicina nuclear, tiroides, tecnología nuclear en salud, SPECT

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Introduction: The images obtained by nuclear medicine allow to visualize the structure and function of an organ, tissue, bone or system inside the body, including the thyroid gland, which can present a thyroid nodule and this is important to diagnose for its potential malignancy. Objective: To determine the functional characteristics of the thyroid nodules were determined in 183 patients, who attended the Nuclear Medicine Service at the Instituto de Investigaciones en Ciencias de la Salud during the period of November 2016 - November 2019. Methodology: It was used the dual head SPECT gamma camera following a standardized protocol with administration of sodium pertechnetate. Result: It was observed that most of the patients corresponded to the female sex 89 %, as well as that 64% came from the capital and the central department. The pathologies detected corresponded to goiter in 82%, 15% to nodules (hypercaptant, hypocaptant and autonoms) and particular cases of thyroiditis, ectopic thyroid and toxic adenoma. Nuclear medicine is directly involved both in the diagnosis and in the treatment of thyroid disease, therefore it requires an understanding of the pathophysiology and management of thyroid disorders in order that public health policies are implemented for strengthening the fight against these diseases. Conclusion: The research carried out showed that a majority percentage of female participants (89%), with thyroid diseases attended IICS / UNA, and the frequency of goiter among all patients who participated in this study was 151/183 ( 82%), the patients mostly they came the reference hospitals in the Central department and coincide with the geographic location of the patients' homes, who tested mainly from the same Central department 118/183 (64%), also participate in the research work patients from other departments of the country. Keywords: Diagnosis, nuclear medicine, thyroid, health technology, SPECT.

Assuntos

Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Nódulo da Glândula Tireoide/diagnóstico por imagem , Paraguai , Doenças da Glândula Tireoide/diagnóstico por imagem , Estudos Transversais , Nódulo da Glândula Tireoide/fisiopatologia , Nódulo da Glândula Tireoide/epidemiologia , Distribuição por Sexo , Distribuição por Idade

RESUMO

INTRODUCTION: In Mexico, physicians have become part of public service prehospital care. Head injured patients are a sensitive group that can benefit from early advanced measures to protect the airway, with the objective to reduce hypoxia and maintain normocapnia. PROBLEM: The occurrence of endotracheal intubation to patients with severe head injuries by prehospital physicians working at Mexico City's Service of Emergency Medical Care (SAMU) is unknown. METHODS: A retrospective analysis of five-year data (2012-2016) from Mexico City's Medical Emergencies Regulation Center was performed. Only SAMU ambulance services were analyzed. Adult patients with a prehospital diagnosis of head injury based on mechanism of injury and physical examination with a Glasgow Coma Scale (GCS)

Assuntos

Traumatismos Craniocerebrais/epidemiologia , Serviços Médicos de Emergência/estatística & dados numéricos , Intubação Intratraqueal/estatística & dados numéricos , Padrões de Prática Médica/estatística & dados numéricos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Traumatismos Craniocerebrais/patologia , Traumatismos Craniocerebrais/terapia , Feminino , Escala de Coma de Glasgow , Humanos , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem

RESUMO

The southern coastline of South America is a remarkable area to evaluate how Quaternary glacial processes impacted the demography of the near-shore marine biota. Here we present new phylogeographic analyses in the pulmonate Siphonaria lessonii across its distribution, from northern Chile in the Pacific to Uruguay in the Atlantic. Contrary to our expectations, populations from the southwestern Atlantic, an area that was less impacted by ice during glacial maxima, showed low genetic diversity and evidence of recent expansion, similar to the patterns recorded in this study across heavily ice-impacted areas in the Pacific Magellan margin. We propose that Atlantic and Pacific shallow marine hard-substrate benthic species were both affected during the Quaternary in South America, but by different processes. At higher latitudes of the southeast Pacific, ice-scouring drastically affected S. lessonii populations compared to non-glaciated areas along the Chile-Peru province where the species was resilient. In the southwest Atlantic, S. lessonii populations would have been dramatically impacted by the reduction of near-shore rocky habitat availability as a consequence of glacio-eustatic movements. The increase of gravelly and rocky shore substrates in the southwest Atlantic supports a hypothesis of glacial refugia from where the species recolonized lower latitudes across the Atlantic and Pacific margins. Our results suggest that current patterns of genetic diversity and structure in near-shore marine benthic species do not solely depend on the impact of Quaternary glacial ice expansions but also on the availability of suitable habitats and life-history traits, including developmental mode, bathymetry and the likelihood of dispersal by rafting.

Assuntos

Ecossistema , Gastrópodes/genética , Variação Genética , Camada de Gelo , Elevação do Nível do Mar , Animais , Oceano Atlântico , Chile , Genética Populacional , Peru , Filogenia , Filogeografia , Uruguai

RESUMO

Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes (bla KPC, bla NDM, bla VIM and bla OXA-48), extended spectrum ß-lactamases (bla SHV-2, bla CTX-M) and the virulence genes (magA, k2A, rmpA, wabG, uge, allS, entB, ycfM, kpn, wcaG, fimH, mrkD, iutA, iroN, hly and cnf-1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The bla KPC-2 gene was present in all the isolates, coexisting with bla CTX-M-2 (45.7%), bla SHV-2 (28.6%), and bla CTX-M-2/bla SHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance (wabG 48.5%, uge 80% and ycfM 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.

RESUMO

The possibility of using electrocoagulation for efficient removal of pollutants in the industrial liquid waste of a textile industry was studied. The performance of the process was evaluated through the analysis of color, turbidity, and chemical oxygen demand (COD). The analysis was first done with the wastewater coming from the process of dyeing linen, which is the most polluting of all effluents that reach the residual effluent pool (REP). For the analysis, the MODDE 7.0 software was used to construct a statistical model. With the results obtained from this model and the experimental measurements, response surfaces were obtained. These response surfaces predicted the behavior of electrocoagulation for different values of the studied variables (pH, current density, and treatment time). Based on the obtained results, the wastewater coming from the REP was treated using the optimum values for the operational variables. After the treatment it was possible to remove 86% color, 82% turbidity, and 59% COD. It was demonstrated that reusing the treated water in the process of wool dyeing does not have a negative effect on the quality of the dyed fabric. Thus, it is possible to implement the process in the textile industry to reduce the consumption of water.

RESUMO

Hookworms of the genus Uncinaria parasitize pinniped pups in various locations worldwide. Four species have been described, two of which parasitize pinniped pups in the southern hemisphere: Uncinaria hamiltoni parasitizes Otaria flavescens and Arctocephalus australis from the South American coast, and Uncinaria sanguinis parasitizes Neophoca cinerea from the Australian coast. However, their geographical ranges and host specificity are unknown. Uncinaria spp. are morphologically similar, but molecular analyses have allowed the recognition of new species in the genus Uncinaria. We used nuclear genetic markers (internal transcribed spacer (ITS) and large subunit (LSU) rDNA) and a mitochondrial genetic marker (cytochrome c oxidase subunit I (COI)) to evaluate the phylogenetic relationships of Uncinaria spp. parasitizing A. australis and O. flavescens from South American coasts (Atlantic and Pacific coasts). We compared our sequences with published Uncinaria sequences. A Generalized Mixed Yule Coalescent (GMYC) analysis was also used to delimit species, and principal component analysis was used to compare morphometry among Uncinaria specimens. Parasites were sampled from A. australis from Peru (12°S), southern Chile (42°S), and the Uruguayan coast, and from O. flavescens from northern Chile (24°S) and the Uruguayan coast. Morphometric differences were observed between Uncinaria specimens from both South American coasts and between Uncinaria specimens from A. australis in Peru and southern Chile. Phylogenetic and GMYC analyses suggest that south-eastern Pacific otariid species harbour U. hamiltoni and an undescribed putative species of Uncinaria. However, more samples from A. australis and O. flavescens are necessary to understand the phylogenetic patterns of Uncinaria spp. across the South Pacific.

Assuntos

Ancylostomatoidea/crescimento & desenvolvimento , Ancylostomatoidea/isolamento & purificação , Caniformia/parasitologia , Infecções por Uncinaria/veterinária , Ancylostomatoidea/classificação , Ancylostomatoidea/genética , Animais , Chile , DNA de Helmintos/genética , DNA Espaçador Ribossômico/genética , Otárias/parasitologia , Infecções por Uncinaria/parasitologia , Peru , Filogenia

RESUMO

Porcine circovirus 3 (PCV-3) is an emerging circovirus species that has recently been reported in different countries around the world, suggesting a widespread circulation. In this study, sera samples originating from 654 pigs of different production phases and clinical/pathological conditions, submitted for diagnostic purposes between 1996 and 2017, were randomly selected. Detection of PCV-3 genome in such samples was attempted with a previously described PCR method, and the partial genome sequence was obtained from selected PCV-3-positive samples from different years. Compiled data confirmed that PCV-3 has been circulating in the Spanish pig population since 1996. The overall frequency of PCV-3 PCR-positive samples in the study period was 11.47% (75 of 654). Phylogenetic analysis of twelve PCV-3 partial sequences obtained showed a high nucleotide identity with the already known PCV-3 sequences, with minor variations among years. No significant correlation was found between the detection of PCV-3 and any production phase nor clinical/pathological condition. These results confirm PCV-3 circulation at least since 1996 in the Spanish pig population with a low/moderate frequency. Although the information obtained was limited, PCV-3 did not appear to be linked to any specific pathological condition or age group.

Assuntos

Infecções por Circoviridae/veterinária , Circovirus/isolamento & purificação , Doenças dos Suínos/epidemiologia , Animais , Sequência de Bases , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/virologia , Circovirus/genética , DNA Viral/genética , Filogenia , Reação em Cadeia da Polimerase/veterinária , Estudos Retrospectivos , Espanha/epidemiologia , Suínos , Doenças dos Suínos/virologia

RESUMO

The presence of the telescope fish Mendosoma lineatum in Patagonian waters of Argentina is reported. Mendosoma lineatum is the second species of the Latridae recorded in the south-west Atlantic Ocean and its presence in Patagonia is an addition to both the ichthyological fauna of the Argentinean Sea and the south-west Atlantic Ocean.

Assuntos

Peixes/classificação , Animais , Argentina , Oceano Atlântico , Peixes/fisiologia , Movimentos da Água

RESUMO

4-nitroquinoline-1-oxide (4-NQO) is a pro-oxidant carcinogen bioactivated by xenobiotic metabolism (XM). We investigated if antioxidants lycopene [0.45, 0.9, 1.8 µM], resveratrol [11, 43, 172 µM], and vitamin C [5.6 mM] added or not with FeSO4 [0.06 mM], modulate the genotoxicity of 4-NQO [2 mM] with the Drosophila wing spot test standard (ST) and high bioactivation (HB) crosses, with inducible and high levels of cytochromes P450, respectively. The genotoxicity of 4-NQO was higher when dissolved in an ethanol - acetone mixture. The antioxidants did not protect against 4-NQO in any of both crosses. In the ST cross, resveratrol [11 µM], vitamin C and FeSO4 resulted in genotoxicity; the three antioxidants and FeSO4 increased the damage of 4-NQO. In the HB cross, none of the antioxidants, neither FeSO4, were genotoxic. Only resveratrol [172 µM] + 4-NQO increased the genotoxic activity in both crosses. We concluded that the effects of the antioxidants, FeSO4 and the modulation of 4-NQO were the result of the difference of Cyp450s levels, between the ST and HB crosses. We propose that the basal levels of the XM's enzymes in the ST cross interacted with a putative pro-oxidant activity of the compounds added to the pro-oxidant effects of 4-NQO.

Assuntos

4-Nitroquinolina-1-Óxido/toxicidade , Ácido Ascórbico/farmacologia , Carotenoides/farmacologia , Compostos Ferrosos/farmacologia , Estilbenos/farmacologia , Animais , Antioxidantes/farmacologia , Ácido Ascórbico/efeitos adversos , Carcinógenos/toxicidade , Carotenoides/efeitos adversos , Sistema Enzimático do Citocromo P-450/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Drosophila melanogaster/efeitos dos fármacos , Drosophila melanogaster/genética , Feminino , Compostos Ferrosos/efeitos adversos , Larva/efeitos dos fármacos , Licopeno , Masculino , Resveratrol , Estilbenos/efeitos adversos , Testes de Toxicidade/métodos , Asas de Animais/efeitos dos fármacos , Xenobióticos/toxicidade

RESUMO

Haematopoietic cell transplant activity in the 28 countries comprising Latin America is poorly defined. We conducted a voluntary survey of members of the Latin American Bone Marrow Transplantation Group regarding transplant activity 2009-2012. Collated responses were compared with data of transplant rates from the Worldwide Network for Blood and Marrow Transplantation for other geographic regions. Several socio-economic variables were analysed to determine correlations with transplant rates. In total, 94 teams from 12 countries reported 11 519 transplants including 7033 autotransplants and 4486 allotransplants. Annual activity increased from 2517 transplants in 2009 to 3263 in 2012, a 30% increase. Median transplants rate (transplant per million inhabitants) in 2012 was 64 (autotransplants, median 40; allotransplants, median 24). This rate is substantially lower than that in North America and European regions (482 and 378) but higher than that in the Eastern Mediterranean and Asia Pacific regions (30 and 45). However, the Latin America transplant rate is 5-8-fold lower than that in America and Europe, suggesting a need to increase transplant availability. Transplant team density in Latin America (teams per million population; 1.8) is 3-4-fold lower than that in North America (6.2) or Europe (7.6). Within Latin America, there is substantial diversity in transplant rates by country partially explained by diverse socio-economic variables including per capita gross national income, health expenditure and physician density. These data should help inform future health-care policy in Latin America.

Assuntos

Transplante de Células-Tronco Hematopoéticas/tendências , Atenção à Saúde/estatística & dados numéricos , Atenção à Saúde/tendências , Previsões , Saúde Global/estatística & dados numéricos , Saúde Global/tendências , Transplante de Células-Tronco Hematopoéticas/estatística & dados numéricos , Humanos , América Latina , Fatores Socioeconômicos , Inquéritos e Questionários

RESUMO

To present an uncommon disturbance of root development with ingrowth of bone and periodontal tissue into the pulp space in a delayed replanted immature permanent incisor at five years of follow-up. Severe arrest of root formation with uncommon healing of an immature permanent maxillary incisor after delayed replantation is reported. Continued development of a tooth root separate from the body of the tooth was not observed. A 6-year-old girl sustained an avulsion injury to her upper left central permanent incisor. The tooth was replanted and splinted 2 hours after the accident. Patient has been monitored clinically and with radiography for 5 years. The periodontal tissues presented good healing without replacement resorption. However, severe arrest of root formation with development of hard-like tissue and internal periodontal ligament inside the pulp canal have been observed. Despite being a delayed tooth replantation, the patient has been asymptomatic, and has maintained alveolar bone volume. Clinicians must be vigilant and monitor traumatized immature teeth closely.

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El objetivo de este trabajo fue presentar una alteración poco frecuente del desarrollo de la raíz con el crecimiento del tejido óseo y periodontal en el espacio pulpar en un incisivo permanente inmaduro reimplantado de manera tardía, a los cinco años de seguimiento.Se observó una suspensión severa de la formación de raíces con cicatrización poco común, de un incisivo maxilar permanente inmaduro después de una reimplantación tardía. No se observó el desarrollo continuo de la raíz dentaria separada del diente. Una niña de 6 años de edad sufrió una lesión por avulsión en su incisivo central superior izquierdo. El diente fue replantado dos horas después del accidente. La paciente ha sido monitoreada clínicamente y con exámenes radiológicos durante 5 años. Los tejidos periodontales presentaron buena cicatrización sin reabsorción de reemplazo. Sin embargo, se ha observado una detención severa de la formación de las raíces, con desarrollo de tejido duro y ligamento periodontal interno dentro del canal pulpar. A pesar de ser una reimplantación tardía del diente, la paciente presenta asintomática, y ha mantenido el volumen del hueso alveolar. Los médicos deben estar atentos y mantener en observación los dientes inmaduros traumatizados.

Assuntos

Humanos , Feminino , Criança , Incisivo/lesões , Avulsão Dentária , Raiz Dentária/crescimento & desenvolvimento , Reimplante Dentário , Raiz Dentária/diagnóstico por imagem

RESUMO

Ageing is characterized by a progressive loss of complexity, which is an essential condition for making the organism capable of keeping homeostasis. Thus, senile loss of complexity makes old individuals frail: a syndrome characterized by the presence of shrinking (sarcopenia), weakness, poor endurance and energy, slowness, and low physical activity. Moreover, renal ageing progressively leads to a glomerular filtration rate (GFR) reduction, one of the main pharmacokinetic senile changes, which is not detectable by simply evaluating serum urea or creatinine values but measuring or calculating patient's GFR. Finally, current epidemiology has documented that detrimental social-behavioral factors such as low education level, poor financial-resource, depression, and isolation, also influence the onset and progression of chronic diseases, and even overall mortality, particularly in the elderly. Thus, we propose that these 3 variables: frailty phenotype, senile GFR, and detrimental social-behavioral factors, should be considered at time of prescribing drugs or medical procedures in the elderly. Additionally, they should also be considered for following patient's response to prescribed therapies in elderly patients suffering from chronic diseases (diabetes mellitus, chronic kidney disease, etc.), or on organ replacement treatments (dialysis and transplantation).

Assuntos

Envelhecimento , Doença Crônica/tratamento farmacológico , Progressão da Doença , Idoso Fragilizado/psicologia , Fatores Socioeconômicos , Idoso de 80 Anos ou mais , Depressão/fisiopatologia , Escolaridade , Taxa de Filtração Glomerular/fisiologia , Humanos , Isolamento Social

RESUMO

We describe a device designed and built to obtain the three-component, steady state velocity field in the whole volume occupied by a fluid in motion contained in a cavity with cylindrical walls. The prototype comprises a two-camera stereoscopic particle image velocimetry system mounted on a platform that rotates around the volume under analysis and a slip ring arrangement that transmits data from the rotating sensors to the data storage elements. Sample observations are presented for natural convection in a cylindrical container but other flows can be analyzed.