RESUMO
Trichomonas vaginalis causes trichomoniasis, an inflammatory process related to an increased rate of HIV transmission. In order to study T. vaginalis infection response in a microorganism-free environment, an infection model was established providing a hostparasite interaction system useful to study the interplay between immune cells and the parasite. Infected mice peritoneal cells were immunophenotyped at different times after infection using flow cytometry. Neutrophils and macrophages showed the most relevant increase from third to 12th day post-infection. A high number of B lymphocytes were present on 15th day post-infection, and an increase in memory T cells was observed on sixth day post-infection. The levels of NO increased at day 10 post-infection; no significant influence was observed on T. vaginalis clearance. Increased viability of T. vaginalis was observed when the NETs inhibitors, metformin and Cl− amidine, were administrated, highlighting the importance of this mechanism to control parasite infection (43 and 86%, respectively). This report presents a comprehensive cell count of the immune cells participating against trichomoniasis in an in vivo interaction system. These data highlight the relevance of innate mechanisms such as specific population changes of innate immune cells and their impact on the T. vaginalis viability.
Assuntos
Tricomoníase , Trichomonas vaginalis , Animais , Cinética , Camundongos , Neutrófilos , PeritônioRESUMO
ABSTRACT The present study was conducted to evaluate the effects of phytogenic additives (PA) and organic acids (OA), alone or in combination, on the performance, intestinal histomorphometry and lipid oxidation, and immune responses of broiler chickens. In this experiment, 820 one-day-old chicks were distributed according to a completely randomized design in a 2 × 2 + 1 factorial arrangement, with four replicates of 41 broilers each. The dietary treatments consisted of a control diet with no PA or OA (CD); CD with OA and no PA (CD+OA-PA); CD with PA and no OA (CD+PA-CD); CDwith both PA and OA (CD+PA+CD); and CD + avilamycin + monesin sodium. Broiler performance was not affected by the alternative feed additives, except from 1 to 21 days, when broilers fed the CD or CD+PA+OA diets showed higher body weight gain than those fed the CD with only OA. The broilers fed the diet containing avilamycin and monensin presented better performance. The supplementation of PA and OA increased bursalcortical area on21 and 42 days post-hatch. On 21 days post-hatch, broilers fed the AGP diet presented higher ileal villus height than those fed the control diet. The pH values of the jejunum content were reduced on the OA-fed chickens. Higher villus height and crypt depth were found in the alternative additive-fed chickens on 7 days post-hatch. On 42 days post-hatch, the percentage of the bursal cortex increased in PA-fed broilers; however, there was no increase in antibody production. The PA-fed chickens presented lower thiobarbituric acid reactive substances (TBARS) values in the small intestine. The dietary supplementation of phytogenic additives, individually or in combination associated with organic acids, does not affect broiler live performance or intestinal histomorphometry; however, it enhances immune responses and intestinal quality.
Assuntos
Animais , Aves Domésticas/fisiologia , Aves Domésticas/metabolismo , Antineoplásicos FitogênicosRESUMO
ABSTRACT The present study was conducted to evaluate the effects of phytogenic additives (PA) and organic acids (OA), alone or in combination, on the performance, intestinal histomorphometry and lipid oxidation, and immune responses of broiler chickens. In this experiment, 820 one-day-old chicks were distributed according to a completely randomized design in a 2 × 2 + 1 factorial arrangement, with four replicates of 41 broilers each. The dietary treatments consisted of a control diet with no PA or OA (CD); CD with OA and no PA (CD+OA-PA); CD with PA and no OA (CD+PA-CD); CDwith both PA and OA (CD+PA+CD); and CD + avilamycin + monesin sodium. Broiler performance was not affected by the alternative feed additives, except from 1 to 21 days, when broilers fed the CD or CD+PA+OA diets showed higher body weight gain than those fed the CD with only OA. The broilers fed the diet containing avilamycin and monensin presented better performance. The supplementation of PA and OA increased bursalcortical area on21 and 42 days post-hatch. On 21 days post-hatch, broilers fed the AGP diet presented higher ileal villus height than those fed the control diet. The pH values of the jejunum content were reduced on the OA-fed chickens. Higher villus height and crypt depth were found in the alternative additive-fed chickens on 7 days post-hatch. On 42 days post-hatch, the percentage of the bursal cortex increased in PA-fed broilers; however, there was no increase in antibody production. The PA-fed chickens presented lower thiobarbituric acid reactive substances (TBARS) values in the small intestine. The dietary supplementation of phytogenic additives, individually or in combination associated with organic acids, does not affect broiler live performance or intestinal histomorphometry; however, it enhances immune responses and intestinal quality.(AU)
Assuntos
Animais , /anatomia & histologia , Aves Domésticas/metabolismo , Aves Domésticas/fisiologia , Antineoplásicos FitogênicosAssuntos
Diabetes Mellitus/fisiopatologia , Neutrófilos/fisiologia , Fagocitose , Adulto , Diabetes Mellitus/sangue , Feminino , Humanos , Látex , Masculino , Pessoa de Meia-IdadeRESUMO
Se estudio la funcion fagocitica de los leucocitos polimorfonucleares de pacientes diabeticos y se comparo con la de controles sanos mediante la prueba de la fagocitosis de particulas de latex. Encontramos una disminucion significativa en el numero de particulas fagocitadas por cada celula y en el porcentaje de celulas fagociticas en pacientes diabeticos; esta alteracion celular seguramente favorece la frecuencia de padecimientos infecciosos en estos pacientes
Assuntos
Diabetes Mellitus , Neutrófilos , FagocitoseRESUMO
The lymphocyte population was studied in 15 patients with rheumatoid arthritis and 15 healthy controls. The early E rosettes, and the B lymphocytes (immunofluorescence) were normal. The late rosettes were lower in rheumatoid arthritis (p less than 0.005). This decrease was correlated with immunosuppressive therapy or the activity of the disease.
Assuntos
Artrite Reumatoide/imunologia , Linfócitos B/imunologia , Linfócitos T/imunologia , Artrite Reumatoide/tratamento farmacológico , Imunofluorescência , Humanos , Formação de Roseta , Fatores de TempoRESUMO
Se estudiaron las poblaciones de linfocitos en sangre periferica de 15 pacientes con artritis reumatoide y 15 controles sanos. La fraccion de linfocitos T formadores de rosetas tempranas y los linfocitos B (inmunofluorescencia) fueron semajantes en ambos grupos, mientras que la fraccion de linfocitos T formadores de rosetas tardias mostro una dismunicion significativa en pacientes con artritis reumatoide (p< 0,005). Esto se atribuyo a la actividad clinica o a la terapeutica inmunosupresora que tenian cinco de los enfermos