RESUMO
Abstract Introduction: Obesity is thought to play a role in the disruption of cardiac rhythmicity in obese children, but this is mostly an unexplored field of investigation. We aimed to evaluate the impact of overweight and obesity on circadian and ultradian cardiovascular rhythmicity of prepubertal children, in comparison with normal weight counterparts. Methods: We performed a cross sectional study of 316 children, followed in the birth cohort Generation XXI (Portugal). Anthropometrics and 24-hour ambulatory blood pressure were measured and profiles were examined with Fourier analysis for circadian and ultradian blood pressure (BP) and heart rate (HR) rhythms. Results: Overweight/obese children presented more frequently a non-dipping BP pattern than normal weight counterparts (31.5% vs. 21.6%, p = 0.047). The prevalence of 24-hour mean arterial pressure (MAP) and 8-hour HR rhythmicity was significantly lower in obese children (79.3% vs. 88.0%, p = 0.038 and 33.3% vs. 45.2%, p = 0.031, respectively). The prevalence of the remaining MAP and HR rhythmicity was similar in both groups. No differences were found in the median values of amplitudes and acrophases of MAP and HR rhythms. Discussion: The alterations found in rhythmicity suggest that circadian and ultradian rhythmicity analysis might be sensitive in detecting early cardiovascular dysregulations, but future studies are needed to reinforce our findings and to better understand their long-term implications.
Resumo Introdução: Acredita-se que a obesidade desempenhe um papel na desregulação da ritmicidade cardíaca em crianças obesas, mas esse é um campo de investigação ainda pouco explorado. O objetivo deste trabalho foi avaliar o impacto do sobrepeso e da obesidade na ritmicidade cardiovascular circadiana e ultradiana de crianças pré-púberes, em comparação com crianças com peso normal. Métodos: Realizamos um estudo transversal com 316 crianças, acompanhadas na coorte de nascimentos Geração XXI (Portugal). Foram medidos dados antropométricos e a pressão arterial ambulatorial de 24 horas, e os perfis foram examinados com uma análise de Fourier para ritmos circadianos e ultradianos de pressão arterial (PA) e frequência cardíaca (FC). Resultados: Crianças com sobrepeso/obesidade apresentaram mais frequentemente um padrão de PA não-dipper em comparação com crianças com peso normal (31,5% vs. 21,6%; p = 0,047). A prevalência da pressão arterial média (PAM) de 24 horas e da ritmicidade da FC de 8 horas foi significativamente menor em crianças obesas (79,3% vs. 88,0%; p = 0,038 e 33,3% vs. 45,2%; p = 0,031, respectivamente). A prevalência das restantes ritmicidades da PAM e da FC foi semelhante em ambos os grupos. Não foram encontradas diferenças nos valores medianos das amplitudes e acrofases dos ritmos de PAM e FC. Discussão: As alterações encontradas na ritmicidade sugerem que a análise da ritmicidade circadiana e ultradiana pode ser sensível na detecção de desregulações cardiovasculares precoces, mas são necessários novos estudos para reforçar nossos achados e entender melhor suas implicações a longo prazo.
RESUMO
INTRODUCTION: Obesity is thought to play a role in the disruption of cardiac rhythmicity in obese children, but this is mostly an unexplored field of investigation. We aimed to evaluate the impact of overweight and obesity on circadian and ultradian cardiovascular rhythmicity of prepubertal children, in comparison with normal weight counterparts. METHODS: We performed a cross sectional study of 316 children, followed in the birth cohort Generation XXI (Portugal). Anthropometrics and 24-hour ambulatory blood pressure were measured and profiles were examined with Fourier analysis for circadian and ultradian blood pressure (BP) and heart rate (HR) rhythms. RESULTS: Overweight/obese children presented more frequently a non-dipping BP pattern than normal weight counterparts (31.5% vs. 21.6%, p = 0.047). The prevalence of 24-hour mean arterial pressure (MAP) and 8-hour HR rhythmicity was significantly lower in obese children (79.3% vs. 88.0%, p = 0.038 and 33.3% vs. 45.2%, p = 0.031, respectively). The prevalence of the remaining MAP and HR rhythmicity was similar in both groups. No differences were found in the median values of amplitudes and acrophases of MAP and HR rhythms. DISCUSSION: The alterations found in rhythmicity suggest that circadian and ultradian rhythmicity analysis might be sensitive in detecting early cardiovascular dysregulations, but future studies are needed to reinforce our findings and to better understand their long-term implications.
Assuntos
Hipertensão , Obesidade Infantil , Humanos , Criança , Sobrepeso/complicações , Obesidade Infantil/complicações , Monitorização Ambulatorial da Pressão Arterial , Estudos Transversais , Ritmo Circadiano/fisiologia , Pressão Sanguínea/fisiologiaRESUMO
Este artigo tem por objetivo identificar e discutir alguns estratagemas psicológicos utilizados por movimentos conservadores e autoritários recentemente difundidos no Brasil - em especial, pelo Movimento Escola sem Partido -, em relação a temas como sexualidade e gênero, que atualmente foram incluídos como essenciais à formação escolar. Com esse propósito, empenhamo-nos em compreender a perspectiva cultural em que se apoiam e o modo como a articulam, ideologicamente, para inviabilizar o debate sobre eles. A partir da análise dos Projetos de Lei 246/2019 e 1859/2015, identificamos estratégias conservadoras que, autoritariamente, deslegitimam sua inclusão na formação escolar. Dentre elas, pareceu-nos urgente investigar a instrumentalização da religião, pois favorece a subordinação da crença religiosa, sobretudo associada ao conservadorismo moral imbricado na tradição cristã brasileira, ao discurso político autoritário. Assim como os movimentos fascistas que, nos Estados Unidos da década de 1930, reivindicavam um ordenamento autoritário e opressor da sociedade por meio do apelo a conteúdos religiosos instrumentalizados para esse fim, atualmente, o discurso religioso também é utilizado como forma de suscitar adesão ao conservadorismo social e político e de justificar preconceitos arraigados. Constatamos que a instrumentalização da religião é uma forma de justificar a permanência de valores conservadores na escola e na sociedade, bem como de reiterar o modelo de família heterossexual monogâmica e a ordem patriarcal. Por meio de estratagemas como esses, os movimentos conservadores e autoritários, articulados em função da negação da diversidade sexual e de gênero, impedem que a escola se constitua como espaço democrático e diverso.(AU)
This paper identifies and discusses the psychological ploys employed by recent conservative and authoritarian movements in Brazil, particularly the School without Party movement, against topics like sexuality and gender, which are currently included as essential to school education. To do so, we sought to understand their cultural basis and how they are ideologically articulated to prevent school debate around these topics. By analyzing Bills 246/2019 and 1859/2015, we identified some conservative strategies that authoritatively delegitimize their inclusion in school education. Chief among them is the instrumentalization of religion, since it favors subordinating religious belief, mainly associated with traditional Christian moral conservatism, to authoritarian political discourse. Similar to the fascist movements in the 1930s United States that claimed an authoritarian and oppressive ordering of society by appealing to religious content, religious speech is currently instrumentalized to encourage social and political conservatism adherence and to justify deep-seated prejudices. Religion instrumentalization is used to justify upholding conservative values at school and in society, as well as to reiterate the monogamous heterosexual family model and patriarchy. Through such ploys, authoritarian and conservative movements, articulated around denying sexual and gender diversity, prevent the school from becoming a democratic and diverse environment.(AU)
Este artículo tiene por objetivo identificar y discutir algunas de las estratagemas psicológicas que utilizan los movimientos conservadores y autoritarios, difundidas recientemente en Brasil, en particular por el Movimiento Escuela sin Partido, con relación a temas como sexualidad y género, que actualmente se incluyeron en la formación escolar. Con este propósito, se pretende comprender la base cultural en la que se han apoyado y cómo la articulan ideológicamente para hacer inviable el debate sobre ellas. A partir de análisis de los Proyectos de Ley 246/2019 y 1859/2015, se identificaron estrategias conservadoras que, autoritariamente, deslegitiman la inclusión de estos temas en la formación escolar. Entre ellas, parece urgente analizar la instrumentalización de la religión, porque favorece la subordinación de la creencia religiosa al discurso político autoritario, sobre todo asociada al conservadurismo moral presente en las vertientes del cristianismo brasileño. Al igual que los movimientos fascistas en los Estados Unidos en los años 1930 que reivindicaban una planificación autoritaria de la sociedad mediante un llamado a contenidos religiosos instrumentalizados, actualmente se utiliza el discurso religioso como forma de promover la adhesión al conservadurismo social y político y de justificar los prejuicios. Se constata que la instrumentalización de la religión es un modo de justificar la permanencia de valores conservadores en la escuela, así como de confirmar el modelo de familia heterosexual monógama y el orden patriarcal. Estos estratagemas que son utilizados por los movimientos conservadores y autoritarios, articulados en función de la negación de la diversidad sexual y de género, impiden que la escuela sea democrática y diversa.(AU)
Assuntos
Humanos , Masculino , Feminino , Política , Religião , Instituições Acadêmicas , Sexualidade , Identidade de Gênero , Sistemas Políticos , Psicologia , Psicologia Social , Política Pública , Racionalização , Papel (figurativo) , Ciência , Educação Sexual , Autoritarismo , Classe Social , Meio Social , Previdência Social , Fatores Socioeconômicos , Estereotipagem , Ensino , Pensamento , Transexualidade , Inclusão Escolar , Bissexualidade , Desenvolvimento Tecnológico , Saúde Mental , Comissão de Ética , Comunismo , Diversidade Cultural , Feminismo , Vida , Discurso , Modernização do Setor Público , Cultura , Capitalismo , Poder Público , Atenção à Saúde , Democracia , Protestantismo , Grupos Raciais , Direitos Sexuais e Reprodutivos , Economia , Educação , Educação Profissionalizante , Escolaridade , Metodologia como Assunto , Estudos Populacionais em Saúde Pública , Saúde Reprodutiva , Sexismo , Tutoria , Fascismo , Ativismo Político , Práticas Interdisciplinares , Etnocentrismo , Extremismo , Opressão Social , Transversalidade de Gênero , Vulnerabilidade Sexual , Normas de Gênero , Binarismo de Gênero , Estudos de Gênero , Políticas Inclusivas de Gênero , Respeito , Políticas Públicas Antidiscriminatórias , Liberdade de Religião , COVID-19 , Governo , Hierarquia Social , Direitos Humanos , Individualidade , Manobras Políticas , Princípios MoraisRESUMO
Invasion of periodontal tissues by Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can be associated with aggressive forms of periodontitis. Oleoresins from different copaifera species and their compounds display various pharmacological properties. The present study evaluates the antibacterial and antivirulence activity of oleoresins obtained from different copaifera species and of ten isolated compounds against two causative agents of periodontitis. The following assays were performed: determination of the minimum inhibitory concentration (MIC), determination of the minimum bactericidal concentration (MBC), and determination of the antibiofilm activity by inhibition of biofilm formation and biofilm eradication tests. The antivirulence activity was assessed by hemagglutination, P. gingivalis Arg-X and Lis-X cysteine protease inhibition assay, and A. actinomycetemcomitans leukotoxin inhibition assay. The MIC and MBC of the oleoresins and isolated compounds 1, 2, and 3 ranged from 1.59 to 50 µg/mL against P. gingivalis (ATCC 33277) and clinical isolates and from 6.25 to 400 µg/mL against A. actinomycetemcomitans (ATCC 43717) and clinical isolates. About the antibiofilm activity, the oleoresins and isolated compounds 1, 2, and 3 inhibited biofilm formation by at least 50% and eradicated pre-formed P. gingivalis and A. actinomycetemcomitans biofilms in the monospecies and multispecies modes. A promising activity concerning cysteine protease and leucotoxin inhibition was also evident. In addition, molecular docking analysis was performed. The investigated oleoresins and their compounds may play an important role in the search for novel sources of agents that can act against periodontal pathogens.
Assuntos
Aggregatibacter actinomycetemcomitans/fisiologia , Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Fabaceae/química , Extratos Vegetais/farmacologia , Porphyromonas gingivalis/fisiologia , Antibacterianos/química , Biofilmes/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Periodontite/tratamento farmacológico , Periodontite/microbiologia , Extratos Vegetais/químicaRESUMO
Down syndrome (DS) is the most common form of mental disability of genetic etiology. Nondisjunction of chromosome 21 is the leading cause of the syndrome. In general, free trisomy 21 cases originate from missegregation in maternal meiosis. Several reports have suggested an association between genetic variants in genes encoding folate metabolizing enzymes and the predisposition to chromosome missegregation. We have conducted a case-control study of 109 DS case mothers (MDS) and 248 control mothers (CM) to assess the association between DHFR del19bp polymorphism and an increased risk of bearing a DS child. Genomic DNA was extracted from buccal cells, and molecular analysis of DHFR del19pb polymorphism was performed by polymerase chain reaction (PCR). Both MDS and CM allelic and genotypic distributions were in Hardy-Weinberg equilibrium. The frequency of DHFR del19pb-mutated allele was 0.54 in MDS and 0.46 in CM. Overall analysis showed that the mutant allele was borderline associated with DS risk (OR 1.38; 95% CI 1.00-1.89; P = 0.05) and a weak positive association for del/del and/or wt/del genotypes of DHFR del19pb polymorphism compared to homozygous wt/wt genotype was identified (OR = 1.75; 95% CI 1.01-3.03; P = 0.05). When we have analyzed data stratified by age, there is an increased risk of bearing a DS child associated with the polymorphic allele (OR = 1.49; 95% CI 1.03-2.16; P = 0.03), suggesting that DHFR del 19-bp polymorphism could be an independent risk factor for DS in women aged < 40 years old.
Assuntos
Síndrome de Down/genética , Polimorfismo Genético , Tetra-Hidrofolato Desidrogenase/genética , Adolescente , Adulto , Fatores Etários , Síndrome de Down/epidemiologia , Feminino , Deleção de Genes , Humanos , Pessoa de Meia-IdadeRESUMO
Ethanol can inhibit ethylene and retard decay of several fruit. However, the effect of ethanol on ethylene and oxidative stress has not yet been observed in bananas. In this work, the effect of ethanol (vapor phase) on physiology and conservation of the banana cv. 'Prata' was investigated. Initially, a group of bananas was analyzed during 8 h of exposure to ethanol, and then another group of bananas was exposed to ethanol for 4 h and then analyzed during 12 days of storage. Ethanol and ethylene concentrations, respiration rate, superoxide dismutase and catalase activities, physico-chemical characteristics and fruit decay were evaluated. Ethanol reduced ethylene production and respiration rate of the banana only when it reached 4 h of exposure, and this was associated with a maximum absorption of ethanol in the fruit peel. Ethanol increased superoxide dismutase and catalase activities from fruit peel only in the first 2 h of exposure to ethanol, i.e. ethanol did not generate significant oxidative stress. During storage, ethanol inhibited ethylene production and was also able to delay fruit decay, but it was not able to influence the respiration rate, conversion of sugars, and fresh weight loss of the fruits. This study showed that ethanol vapor has an inhibitory effect on ethylene metabolism, but it has no potential to control post-harvest ripening of the banana cv. 'Prata'. On the other hand, ethanol delays decay, and this extends fruit shelf life, which is commercially advantageous.
RESUMO
Down syndrome (DS) is the most common form of human genetic mental retardation. Several polymorphisms in genes coding folic acid cycle enzymes have been associated to the risk of bearing a DS child; however, the results are controversial. S-adenosyl-l-methionine (SAM) is an important intermediate of folic acid pathway and acts as methyl donor and substrate for DNA (cytosine-5)-methyltransferase 3B (DNMT3B - EC 2.1.1.37) de novo methylation processes during embryogenesis. Recent studies suggest that a functional polymorphism of DNMT 3B in maternal genotype may be associated with a decreased risk of having a DS child. We herein investigate the association of this polymorphism with the occurrence of DS in a Brazilian population. We have genotyped 111 mothers of DS infants (MDS) and 212 control mothers (CM) through PCR-RFLP. The observed genotypic frequencies were CC = 0.22; CT = 0.49 and TT = 0.29 in CM, and CC = 0.30; CT = 0.52 and TT = 0.18 in MDS. Allelic frequencies were C = 0.47 and T = 0.53 in CM and C = 0.56 and T = 0.44 in MDS. No deviation of HWE was observed, and both DNMT 3B rs2424913 genotype (χ2 = 4.53; DF = 1; P = 0.03) and allelic (χ2 = 4.90; DF = 1; P = 0.03) frequencies show significant differences between MDS and CM. The presence of the mutant DNMT 3B T allele decreases 30% the risk of bearing a DS child (OR = 0.69; 95% CI: 0.50-0.96; P = 0.03), and the risk is diminished up to 45% in association with the homozygous genotype (OR = 0.54; 95% CI: 0.31-0.96; P = 0.04). Our results suggest that women harboring the single nucleotide polymorphism DNMT 3B rs2424913 have a decreased risk of a DS pregnancy, and further studies are necessary to confirm this protective effect.
RESUMO
Alelopatia se refere à capacidade que determinada planta possui de interferir no metabolismo de outra, por meio de compostos químicos liberados no meio. A tiririca é um exemplo típico deste tipo de planta, possuindo elevada concentração de ácido indolbutírico, podendo agir na germinação e enraizamento de outras plantas. Diante do exposto, objetivou-se com o presente trabalho avaliar o efeito de extratos de bulbos e folhas de tiririca e ácido giberélico na germinação de sementes de maracujá-amarelo. Para a obtenção dos extratos aquosos de tiririca utilizaram-se bulbos e folhas na concentração de 1 g 10 mL-1 (p/v) (1 g de matéria vegetal para 10 mL de água destilada), constituindo as soluções estoque. Foram utilizados extratos diluídos em cinco concentrações (20, 40, 60, 80 e 100%) e cinco concentrações de ácido giberélico (200, 400, 600, 800 e 1000 mg L-1 GA3 ). Como tratamento controle utilizou-se água destilada. O delineamento experimental foi inteiramente casualizado, apresentando 16 tratamentos, contendo cinco repetições, totalizando 80 unidades amostrais, contendo cada unidade dez sementes. Diariamente realizaram-se contagens do número de sementes germinadas para posterior cálculo de percentagem de germinação e Índice de Velocidade de Germinação. Sementes de maracujá-amarelo submetidas às concentrações de ácido giberélico tiveram sua germinação inibida, não sendo recomendado na germinação da espécie. Concentrações de extrato aquoso de folhas e bulbos de tiririca não influenciaram a germinação de sementes de maracujá-amarelo.(AU)
Allelopathy refers to the ability of certain plant has to interfere with the metabolism of other compounds by chemicals released into the environment. Cyperus rotundus is a typical example of such a plant, having a high IBA concentration and being able to influence and act on germination and rooting of the other plants. The aim of this study was to evaluate the effect of bulbs and leaves extracts of Cyperus rotundus and gibberellic acid on the yellow passion fruit germination cycle. To obtain the aqueous extract bulbs and leaves were used along with a concentration of 1 g 10 mL-1 (p/v) (1 g of material plant to 10 mL of distilled water), forming stock solutions. Extracts were diluted in five concentrations (20, 40, 60, 80 and 100%) and five concentrations of gibberellic acid (200, 400, 600, 800 and 1000 mg L-1 GA3 ) were used. As a control treatment we used distilled water. The experimental design was completely randomized, with 16 treatments, five replicates, totaling 80 sampling units, each unit containing ten seeds. Daily counts were performed on the number of germinated seeds for subsequent calculation of germination percentage and germination speed index. Seeds of yellow passion fruit treated with concentrations of gibberellic acid had their germination inhibited and it is not recommended in the germination of the species. Concentrations of aqueous extract of leaves and sedge did not affect the germination of yellow passion fruit.(AU)
Assuntos
Germinação/fisiologia , Passiflora , Alelopatia/fisiologia , Extratos Vegetais/análise , Cyperus , Desenvolvimento Vegetal/fisiologiaRESUMO
Alelopatia se refere à capacidade que determinada planta possui de interferir no metabolismo de outra, por meio de compostos químicos liberados no meio. A tiririca é um exemplo típico deste tipo de planta, possuindo elevada concentração de ácido indolbutírico, podendo agir na germinação e enraizamento de outras plantas. Diante do exposto, objetivou-se com o presente trabalho avaliar o efeito de extratos de bulbos e folhas de tiririca e ácido giberélico na germinação de sementes de maracujá-amarelo. Para a obtenção dos extratos aquosos de tiririca utilizaram-se bulbos e folhas na concentração de 1 g 10 mL-1 (p/v) (1 g de matéria vegetal para 10 mL de água destilada), constituindo as soluções estoque. Foram utilizados extratos diluídos em cinco concentrações (20, 40, 60, 80 e 100%) e cinco concentrações de ácido giberélico (200, 400, 600, 800 e 1000 mg L-1 GA3 ). Como tratamento controle utilizou-se água destilada. O delineamento experimental foi inteiramente casualizado, apresentando 16 tratamentos, contendo cinco repetições, totalizando 80 unidades amostrais, contendo cada unidade dez sementes. Diariamente realizaram-se contagens do número de sementes germinadas para posterior cálculo de percentagem de germinação e Índice de Velocidade de Germinação. Sementes de maracujá-amarelo submetidas às concentrações de ácido giberélico tiveram sua germinação inibida, não sendo recomendado na germinação da espécie. Concentrações de extrato aquoso de folhas e bulbos de tiririca não influenciaram a germinação de sementes de maracujá-amarelo.
Allelopathy refers to the ability of certain plant has to interfere with the metabolism of other compounds by chemicals released into the environment. Cyperus rotundus is a typical example of such a plant, having a high IBA concentration and being able to influence and act on germination and rooting of the other plants. The aim of this study was to evaluate the effect of bulbs and leaves extracts of Cyperus rotundus and gibberellic acid on the yellow passion fruit germination cycle. To obtain the aqueous extract bulbs and leaves were used along with a concentration of 1 g 10 mL-1 (p/v) (1 g of material plant to 10 mL of distilled water), forming stock solutions. Extracts were diluted in five concentrations (20, 40, 60, 80 and 100%) and five concentrations of gibberellic acid (200, 400, 600, 800 and 1000 mg L-1 GA3 ) were used. As a control treatment we used distilled water. The experimental design was completely randomized, with 16 treatments, five replicates, totaling 80 sampling units, each unit containing ten seeds. Daily counts were performed on the number of germinated seeds for subsequent calculation of germination percentage and germination speed index. Seeds of yellow passion fruit treated with concentrations of gibberellic acid had their germination inhibited and it is not recommended in the germination of the species. Concentrations of aqueous extract of leaves and sedge did not affect the germination of yellow passion fruit.
Assuntos
Alelopatia/fisiologia , Cyperus , Extratos Vegetais/análise , Germinação/fisiologia , Passiflora , Desenvolvimento Vegetal/fisiologiaRESUMO
Objetivo Identificar a incidência de Citomegalovírus (CMV) em pacientes HIV (Vírus da Imunodeficiência Adquirida) positivos, através de levantamento de dados arquivados de pacientes que apresentaram produção de Imunoglobulina IgG e IgM para CMV e provável positividade para ambos. Métodos Os resultados foram obtidos através de análise de prontuários de pacientes HIV positivos, atendidos em um laboratório de análises clínicas de Jundiaí, São Paulo, cujo atendimento consiste em pacientes da rede particular e do Sistema Único de Saúde (SUS). Os dados foram levantados num retrospecto de 46 meses, entre janeiro de 2011 e outubro de 2014 e foram analisados três parâmetros principais: a média de solicitações de sorologia para HIV, a incidência para CMVM (IgM para Citomegalovírus) e CMVG (IgG para Citomegalovírus) em pacientes HIV positivos e o percentual destes pacientes infectados tanto na rede particular como na rede pública. Resultados No período analisado, 88 pacientes foram diagnosticados com HIV confirmados através de Western Blotting. Destes confirmou-se que 2,27% apresentaram IgM positiva para CMV. Tais pacientes foram atendidos pela rede particular de saúde. Conclusão Isto posto, apesar da TARV (Terapia antirretroviral), ainda há pacientes que se infectam com o CMV. Foi possível avaliar que independente da rede de atendimento o paciente imunocomprometido pelo HIV necessita de atenção especial e acompanhamento minucioso para sobrevida e bem-estar.
Objective To identify the incidence of cytomegalovirus in patients seropositive for HIV, up examinations of patients with IgG and IgM for CMV positivity and possible for both. Methods Through medical records of HIV-positive patients, the data were performed after prior approval of a clinical laboratory in Jundiai, São Paulo, which is to serve the public private network and public network. The data were collected in a retrospective of 46 months between January 2011 and October 2014 and there were three data analysis: the average requests for HIV testing, the incidence for E CMVG CMVM and the percentage of these infected patients in private institutions and the public network. Results Through the medical records, it was found 88 patients with HIV confirmed by Western blotting, it was confirmed that 2.27% were infected with CMV, is have positive IgM and are treated at private. Conclusion That said, despite the HAART (antiretroviral therapy), there are still patients who became infected with CMV, it is possible to assess whether the service network immunocompromised HIV patients need special attention and careful monitoring for survival and well-being.
RESUMO
Down syndrome (DS) is the most common form of mental retardation of genetic etiology. Several polymorphisms in genes involved with the folic acid cycle have been associated to the risk of bearing a DS child; however, the results are controversial. Betaine-homocysteine methyltransferase (BHMT) is a key enzyme of folate pathway, and catalyzes the remethylation of homocysteine into methionine. Recent studies suggest that the polymorphism BHMT 742G>A may be associated with a decreased risk of having a DS child. We herein investigate the association of this polymorphism with the occurrence of DS in a Brazilian population. We have genotyped 94 mothers of DS infants (DSM) and 134 control mothers (CM) for this polymorphism through PCR-RFLP, and found significant differences for both BHMT 742G>A genotype (P=0.04) and allele (P=0.03) frequencies between DSM and CM. The observed genotypic frequencies were GG=0.45; GA=0.45 and AA=0.10 in CM, and GG=0.54; GA=0.38 and AA=0.02 in DSM. Allelic frequencies were G=0.68 and A=0.32 in CM and G=0.78 and A=0.22 in DSM. The presence of the mutant BHMT 742 A allele decreases 40% the risk of bearing a DS child (OR=0.61; 95% CI: 0.40-0.93; P=0.03), and the risk is diminished up to >80% in association with the homozygous genotype (OR=0.17; 95% CI: 0.04-0.80; P=0.01). Our results indicate that women harboring the single nucleotide polymorphism BHMT 742G>A have a decreased risk of a DS pregnancy, and further studies are necessary to confirm this protective effect.
Assuntos
Betaína-Homocisteína S-Metiltransferase/genética , Segregação de Cromossomos/genética , Síndrome de Down/genética , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único/genética , Brasil , Feminino , Frequência do Gene , Genótipo , Humanos , Mães , Razão de Chances , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de RestriçãoRESUMO
Identification of Escherichia coli requires knowledge regarding the prevalent serotypes and virulence factors profiles allows the classification in pathogenic/non-pathogenic. However, some of these bacteria do not express flagellar antigen invitro. In this case the PCR-restriction fragment length polymorphism (RFLP-PCR) and sequencing of the fliC may be suitable for the identification of antigens by replacing the traditional serology. We studied 17 samples of E. coli isolated from animals and presenting antigen H nontypeable (HNT). The H antigens were characterized by PCR-RFLP and sequencing of fliC gene. Three new flagellin genes were identified, for which specific antisera were obtained. The PCR-RFLP was shown to be faster than the serotyping H antigen in E. coli, provided information on some characteristics of these antigens and indicated the presence of new genes fliC.(AU)
A identificação da Escherichia coli requer conhecimento sobre os sorotipos e fatores de virulência prevalentes permitindo a classificação em patogênico/não patogênico. No entanto, algumas destas bactérias não expressam o antígeno flagelar in vitro. Neste caso, o PCR-restriction fragment length polymorphism (RFLP-PCR) e o sequenciamento do gene fliC podem ser adequados para a identificação desses antígenos, substituindo a sorologia tradicional. Nesta pesquisa foram estudadas 17 amostras de E. coli isoladas de animais e que apresentavam antígeno H não tipável (HNT). Os antígenos H foram caracterizados por PCR-RFLP e sequenciamento do gene fliC. Três novos genes da flagelina foram identificados, para os quais anti-soros específicos foram obtidos. A técnica PCR-RFLP mostrou-se mais rápida que a sorotipagem do antígeno H em E. coli, fornecendo informações sobre algumas características desses antígenos e indicou a presença de novos genes fliC.(AU)
Assuntos
Animais , Escherichia coli/isolamento & purificação , Flagelina/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Polimorfismo de Fragmento de Restrição , Sorotipagem/veterinária , AntígenosRESUMO
Identification of Escherichia coli requires knowledge regarding the prevalent serotypes and virulence factors profiles allows the classification in pathogenic/non-pathogenic. However, some of these bacteria do not express flagellar antigen invitro. In this case the PCR-restriction fragment length polymorphism (RFLP-PCR) and sequencing of the fliC may be suitable for the identification of antigens by replacing the traditional serology. We studied 17 samples of E. coli isolated from animals and presenting antigen H nontypeable (HNT). The H antigens were characterized by PCR-RFLP and sequencing of fliC gene. Three new flagellin genes were identified, for which specific antisera were obtained. The PCR-RFLP was shown to be faster than the serotyping H antigen in E. coli, provided information on some characteristics of these antigens and indicated the presence of new genes fliC.
A identificação da Escherichia coli requer conhecimento sobre os sorotipos e fatores de virulência prevalentes permitindo a classificação em patogênico/não patogênico. No entanto, algumas destas bactérias não expressam o antígeno flagelar in vitro. Neste caso, o PCR-restriction fragment length polymorphism (RFLP-PCR) e o sequenciamento do gene fliC podem ser adequados para a identificação desses antígenos, substituindo a sorologia tradicional. Nesta pesquisa foram estudadas 17 amostras de E. coli isoladas de animais e que apresentavam antígeno H não tipável (HNT). Os antígenos H foram caracterizados por PCR-RFLP e sequenciamento do gene fliC. Três novos genes da flagelina foram identificados, para os quais anti-soros específicos foram obtidos. A técnica PCR-RFLP mostrou-se mais rápida que a sorotipagem do antígeno H em E. coli, fornecendo informações sobre algumas características desses antígenos e indicou a presença de novos genes fliC.
Assuntos
Animais , Escherichia coli/isolamento & purificação , Flagelina/isolamento & purificação , Polimorfismo de Fragmento de Restrição , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Antígenos , Sorotipagem/veterináriaRESUMO
INTRODUCTION: The diarrhea associated with gastroenteritis is a major cause of morbidity and mortality worldwide, affecting mainly infants. The characterization of both viral and bacterial agents associated with gastroenteritis can establish policies for surveillance, prevention and treatment of infections. Group A rotaviruses are the major infectious agent associated with dehydration in children, followed by pathotypes of Escherichia coli. There are three main types of clinical infections caused by E. coli strains that have acquired virulence genes: (i) enteric and diarrheal diseases, (ii) urinary tract infections, and (iii) sepsis and meningitis. METHODOLOGY: In this study, the objective was to identify the presence of rotavirus and diarrhogenic E. coli in the feces of children 4 to 14 months of age who displayed no gastroenteritis symptoms and stayed all day in a day-care center. We analyzed 188 samples using PAGE and PCR to identify rotaviruses and E. coli virulence genes, respectively. RESULTS: Thirty-six samples (19.1%) were positive for at least one pathotype of E. coli. Nineteen were identified to be of the EPEC group and fifteen of the EAEC group. Rotaviruses were not identified. CONCLUSIONS: As EPEC and EAEC are potential pathogens for children less than one year of age or immunocompromised individuals, our results show the importance of appropriate monitoring by public health agencies. In the situation that we have studied, children can be considered asymptomatic carriers of these pathogens and can transmit them to other susceptible children.
Assuntos
Portador Sadio/epidemiologia , Portador Sadio/microbiologia , Escherichia coli/isolamento & purificação , Fezes/microbiologia , Rotavirus/isolamento & purificação , Brasil , Creches , Feminino , Humanos , Lactente , Masculino , PrevalênciaRESUMO
Introdução: Segundo a literatura científica, o comportamento alimentar de idosos está relacionado a variáveis sociodemográficas e culturais.Objetivo: Verificar a associação entre comportamento alimentar de idosos, residentes no município de São Paulo, e variáveis sociodemográficas e culturais. Métodos: Estudo transversal, com idosos ( 60 anos), de ambos os sexos, do Estudo SABE: Saúde, Bem-estar e Envelhecimento, realizado em 2000, no município de São Paulo, selecionados por amostra probabilística. As variáveis de estudo foram referidas: comportamento alimentar (número de refeições < 3/dia, ausência de costume para preparar refeições quentes e comprar alimentos, e frequência de ingestão de alimentos.
Assuntos
Humanos , Masculino , Feminino , Idoso , Comportamento Alimentar/psicologia , /psicologia , Psicologia , Brasil , Estudos Transversais , Demografia , Saúde do Idoso , Fatores Socioeconômicos , Área UrbanaRESUMO
CONTEXT: Sydenham's chorea affects almost 30% of patients with acute rheumatic fever. It is more frequent in females and is rare in the first decade of life, and genetic vulnerability underlies it. Because of easy access to antibiotics, it is now rare in so-called developed countries. CASE REPORT: A 6-year-old boy with a family history of Huntington's disease, who was the only child of an unscreened and asymptomatic mother, was brought for a consultation because of migratory arthralgia, depressed mood, and rapid, abrupt and unintentional movements of his right arm and leg, that had evolved over a three-week period. On physical examination, he presented a grade III/VI systolic heart murmur and right-side choreic movements, giving rise to a deficit of active mobilization. Laboratory tests revealed elevated erythrocyte sedimentation rate (63 mm/h), C-reactive protein (25 mg/l) and antistreptolysin O titer (1,824 U/ml). Cardiovascular evaluation showed mild aortic insufficiency, moderate mitral insufficiency and a prolonged PR interval. A clinical diagnosis of Sydenham's chorea/acute rheumatic fever was made, and therapy consisting of penicillin, haloperidol, captopril and furosemide was instituted, with excellent results. CONCLUSION: In developed countries, Sydenham's chorea seems forgotten and, because of this, little is known about its clinical course and controversy surrounds the therapeutic options available. This occurrence of rheumatic chorea in a family with Huntington's disease highlights the importance of the differential diagnosis for the different forms of chorea.
Assuntos
Coreia/diagnóstico , Doença de Huntington/diagnóstico , Criança , Diagnóstico Diferencial , Saúde da Família , Humanos , MasculinoRESUMO
Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and different restriction enzyme profiles were observed. Anti-sera were produced in rabbits, for the non-typeable strains, and agglutination tests were carried out. In conclusion,the results showed that although non-typeable and typable H antigens strains had similar flagellar antigens, the two types of strains were distinct in terms of nucleotide sequence, and did not phenotypically react with the standard antiserum, as expected. Thirteen strains had been characterized as likely putative new H antigen using PCR-RFLP techniques, DNA sequencing and/or serology.
Assuntos
Antígenos de Bactérias/imunologia , Escherichia coli/imunologia , Flagelina/imunologia , Análise de Sequência de DNA/métodos , Animais , Antígenos de Bactérias/genética , Escherichia coli/genética , Flagelina/genética , Humanos , Reação em Cadeia da Polimerase , CoelhosRESUMO
Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and different restriction enzyme profiles were observed. Anti-sera were produced in rabbits, for the non-typeable strains, and agglutination tests were carried out. In conclusion,the results showed that although non-typeable and typable H antigens strains had similar flagellar antigens, the two types of strains were distinct in terms of nucleotide sequence, and did not phenotypically react with the standard antiserum, as expected. Thirteen strains had been characterized as likely putative new H antigen using PCR-RFLP techniques, DNA sequencing and/or serology.
Assuntos
Animais , Humanos , Coelhos , Antígenos de Bactérias/imunologia , Escherichia coli/imunologia , Flagelina/imunologia , Análise de Sequência de DNA/métodos , Antígenos de Bactérias/genética , Escherichia coli/genética , Flagelina/genética , Reação em Cadeia da PolimeraseRESUMO
CONTEXT: Sydenham's chorea affects almost 30 percent of patients with acute rheumatic fever. It is more frequent in females and is rare in the first decade of life, and genetic vulnerability underlies it. Because of easy access to antibiotics, it is now rare in so-called developed countries. CASE REPORT: A 6-year-old boy with a family history of Huntington's disease, who was the only child of an unscreened and asymptomatic mother, was brought for a consultation because of migratory arthralgia, depressed mood, and rapid, abrupt and unintentional movements of his right arm and leg, that had evolved over a three-week period. On physical examination, he presented a grade III/VI systolic heart murmur and right-side choreic movements, giving rise to a deficit of active mobilization. Laboratory tests revealed elevated erythrocyte sedimentation rate (63 mm/h), C-reactive protein (25 mg/l) and antistreptolysin O titer (1,824 U/ml). Cardiovascular evaluation showed mild aortic insufficiency, moderate mitral insufficiency and a prolonged PR interval. A clinical diagnosis of Sydenham's chorea/acute rheumatic fever was made, and therapy consisting of penicillin, haloperidol, captopril and furosemide was instituted, with excellent results. CONCLUSION: In developed countries, Sydenham's chorea seems forgotten and, because of this, little is known about its clinical course and controversy surrounds the therapeutic options available. This occurrence of rheumatic chorea in a family with Huntington's disease highlights the importance of the differential diagnosis for the different forms of chorea.
CONTEXTO: A coreia de Sydenham surge em cerca de 30 por cento dos casos de febre reumática aguda. É mais frequente no sexo feminino, é rara na primeira década de vida e tem por base uma vulnerabilidade genética. Devido ao fácil acesso aos antibióticos, é uma doença rara atualmente nos países ditos desenvolvidos. RELATO DO CASO: Criança de seis anos, sexo masculino, com história familiar de coreia de Huntington, único filho de mãe assintomática e não rastreada, foi trazido à consulta por artralgias migratórias, humor deprimido e movimentos rápidos, abruptos e não intencionais dos membros superior e inferior direitos, com três semanas de evolução. Ao exame físico, apresentava um sopro cardíaco sistólico grau III/VI, e foram presenciados movimentos coreicos à direita, condicionando um défice de mobilização activa. Os exames laboratoriais mostraram aumento da velocidade de sedimentação (63 mm/h), proteína C-reativa (25 mg/L) e título de antiestreptolisina O (1.824 U/mL). O exame cardiovascular revelou insuficiência aórtica ligeira e insuficiência mitral moderada e aumento do intervalo PR. Foi feito o diagnóstico de coreia de Sydenham/febre reumática aguda, tendo sido instituída terapêutica com penicilina, haloperidol, captopril e furosemida, com excelente resultado. CONCLUSÃO: Nos países desenvolvidos, a coreia de Sydenham parece esquecida e, por isso, pouco se sabe quanto ao seu curso clínico e as opções terapêuticas disponíveis são controversas. A ocorrência de um caso de coreia reumática numa família com doença de Huntington realça a importância do diagnóstico diferencial das diferentes formas de coreia.
Assuntos
Criança , Humanos , Masculino , Coreia/diagnóstico , Doença de Huntington/diagnóstico , Diagnóstico Diferencial , Saúde da FamíliaRESUMO
Objetivo: Analisar a rede formal e informal de apoio ao familiar cuidador de pessoa com Atrofia Muscular Espinhal tipos I e II. Método: Trabalho de delineamento qualitativo desenvolvido em 2008, nas residências dos informantes e nas dependências do Hospital Infantil Albert Sabin, em Fortaleza-Ceará, realizado com 13 mães, cuidadoras principais de seus filhos doentes. Para coleta de dados, utilizaram-se a entrevista e o instrumento gerador de nomes e qualificador da relação, adaptados para este estudo. Os dados quantitativos foram processados através dos programas UCINET 6.123 e NetDraw 2.38, enquanto os dados qualitativos foram organizados com base na técnica do Discurso do Sujeito Coletivo. As análises se deram em função dos mapas de rede e dos discursos coletivos suscitados pelas ideias centrais. Resultados: A rede social formal dos Familiares Cuidadores foi composta de 72 atores, entre profissionais das áreas da saúde, educação e outros, vinculados a 12 instituições. A rede informal total era composta por 83 atores. Constatou-se que o gerenciamento do cuidado, mesmo quando o familiar cuidador tem suporte de outras pessoas, é motivo de estresse e sobrecarga. Conclusões: Está evidente a importância da aplicação da Análise de Redes Sociais como ferramenta para conhecimento dos aspectos estruturais e da dinâmica das relações sociais do familiar cuidador de pessoa com Atrofia Muscular Espinhal. A análise realizada sugere necessidade de intervenção junto ao grupo estudado como forma de contribuir para o reconhecimento e utilização dos suportes existentes, potencializando o significado de ajuda das interações em rede.
Objective: To assess the formal and informal support given to the family caregiver of a patient with Spinal Muscular Atrophy I and II. Method: This was a study with a qualitative approach developed in 2008 in the homes of informants and in the premises of the Hospital Infantil Albert Sabin in Fortaleza, Ceara, conducted with 13 mothers, primary caregivers for their ill children. For data collection we used both the interview and the instrument generator of the names and qualifier of the relations, adapted for this study. Quantitative data were processed using the programs UCINET NetDraw 6.123 and 2.38, while the qualitative data were organized based on the technique of the Collective Subject Discourse. The analyses were conducted by means of network maps and collective discourses raised by central ideas. Results: The formal social network of family caregivers was composed of 72 actors, among professionals in the areas of health, education and others, linked to 12 institutions. The informal network was comprised of 83 actors. It was found that the management of care, even when the caregiver has support from other people is a cause of stress and overload. Conclusion: It is evident the importance of applying Social Network Analysis as a tool for understanding structural features and the dynamics of social relations of family caregiver. Of a patient with spinal muscular atrophy. The analysis suggests the need for intervention in the study group as a way to contribute to the recognition and use of existing services, increasing the significance of the help provided by network interactions.