RESUMO
Pathogenic mycobacteria survive inside macrophages and deactivate these cells, using a mechanism that is still poorly understood. Mycobacterial cell wall lipids constitute the first contact with the host cell. Although Mycobaterium leprae and M. bovis BCG share common antigens, they induce opposite inflammatory responses. Apolar M. leprae lipids have been shown to be anti-inflammatory by down-regulating macrophage activation and T-cell functions. We wonder if these lipids would influence cellular migration to BCG or to other inflammatory agent. We investigated the effect of M. leprae, its lipids or delipidated bacteria on acute and chronic BCG- or carrageenan-induced pleurisy. Previous injection of intact or delipidated M. leprae did not alter either the BCG- or carrageenan-induced pleural inflammatory reaction. However, M. leprae lipids enhanced carrageenan-induced acute cellular migration without impairing BCG inflow; moreover, they reduced BCG chronic response. Together these data suggest distinct mechanisms for intracellular deactivation and pleural cell recruitment exerted by mycobacterial structures.
Assuntos
Lipídeos/farmacologia , Mycobacterium leprae/fisiologia , Pleurisia/patologia , Animais , Vacina BCG/farmacologia , Carragenina/farmacologia , Movimento Celular/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos C57BLAssuntos
Animais , Ativação de Macrófagos , Camundongos , Camundongos Endogâmicos BALB C , Células Cultivadas , Células da Medula Óssea , Depressão Química , Explosão Respiratória , Fagocitose , Hanseníase/imunologia , Lipossomos , Lipídeos/farmacologia , Lipídeos/isolamento & purificação , Macrófagos , Macrófagos/fisiologia , Macrófagos/microbiologia , Mycobacterium leprae/química , Parede Celular/química , Superóxidos/metabolismoRESUMO
Monoclonal antibodies (Mabs) were produced aginst (C57B1/6 "nude" lymphoblast cells. They are IgG2a and do not react with mouse serum proteins. Five Mabs were characterixed functional by immunohistochemistry and immunofluorescence in spleen, lymph nodes and Peyer's patches from nude and normal mice, an anormal mouse thymus. Two Mabs reacted with containing B-cells as indicared by immunoperoxidase staining, another recognized T- and B-cell sites and two specifically stained T lymphocyte-containing areas. Immunofluorescence studies confirmed the sites identified above by immunohistochemistry