RESUMO
Heptaldehyde-modified chitosan (heptyl-chitosan, CH-Hp) was investigated as adsorbent for chromatograhic separation of the flavonoids from A. moluccana. The amount of 2"-O-rhamnosylswertisin isolated (30.0 mg) was approx. twice as high as swertisin (17.5 mg). The improved surface hydrophobicity effected by the heptyl groups promoted the separation of flavonoids. From the results obtained, CH-Hp seems to be more suitable for separation of glycosylated flavonoids than other flavonoids. Thus, modified chitosan described here can be used for hydrophobic interaction chromatography as sucessfully illustrated with flavonoids.
Assuntos
Aleurites/química , Flavonoides/química , Oligossacarídeos/química , Folhas de Planta/química , Acetilação , Aldeídos , Sequência de Carboidratos , Quitosana , Cromatografia/métodos , Flavonoides/isolamento & purificação , Indicadores e Reagentes , Dados de Sequência Molecular , Oligossacarídeos/isolamento & purificação , Espectrofotometria Infravermelho/métodosRESUMO
This paper describes a comparative study using different chromatographic supports (fully N-acetylated chitin, chitin and silica gel) to separate the flavonoids swertisin and 2"-O-rhamnosylswertisin from Aleurites moluccana. The results show that the flavonoids have apparently been separated by the hydrogen bond between the stationary phase (chitin and chitin-100) and flavonoids under the conditions studied.