RESUMO
Foram examinadas para pesquisa de Campylobacter, 102 amostras de fezes de cäes de ambulatório com diarréia (0 - 12 meses de idade) e 102 amostras de cäes errantes sem diarréia (com idade indeterminada). No grupo com diarréia, o Campylobacter foi isolado em 46 por cento dos animais, notando-se tendência nos cäes de até cinco meses de idade estarem amis acometidos pelo microorganismo (72 por cento) e de 6 por cento nos com apenas uma semana de vida. As 47 estirpes isoladas dos cäes com diarréia foram classificadas como sendo: C. jejuni biotipo1 (49 por cento); C. jejuni biotipo2 (11 por cento); C. jejuni/coli (19 por cento); C. cli (8.5 por cento), NARTC - grupo (8.5 por cento) e C. sputorum (4 por cento). No grupo sem diarréia a incidência foi de 28 por cento sendo as 27 estirpes isoladas classificadas como: C. jejuni biotipo1 (34 por cento) e biotipo2 (28 por cento); C. jejuni/coli (24 por cento) e C. coli (14 por cento). Nas provas bioquímicas, o teste de tolerância a 1 por cento de glicina falhou em 45 por cento das estirpes classificadas fenotipicamente como C. jejuni e três estirpes näo reduziram o selenito de sódio. Duas estirpes do NARTC-grupo, mostraram ainda reaçöes fenotípicas cruzadas com C. jejuni e C. coli e duas de C. jejuni também cresceram a 25ºC. As 76 estirpes isoladas foram sensíveis à gentamicina, à nitrofurantoína e à neomicina e resistentes à oxacilina e à penicilina. Para as 16 drogas restantes testadas a resistência bacteriana variou entre 8 por cento e 73 por cento das amostras. A presença de Campylobacter nos cäes pode evidenciar o risco de infecçäo ao homem, sendo por esse fato a campilobacteriose também freqüentemente citada como importante zoonose.
Assuntos
Animais , Cães , Cães , Infecções por Campylobacter/diagnóstico , Diarreia , CãesRESUMO
Säo relatados casos de ceratoconjuntivite provocados por Neisseria ovis em rebanho ovino das raças Merino, Ile de France, Ideal, Corriedale e Romny March. Os autores, após identificarem os microrganismo, selecionaram amostras que foram utilizadas para o preparo de vacina. Levando em consideraçäo as provas de patogenicidade experimental, variaçöes biológicas do parasito e fatores que predispöem o globo ocular à infecçäo, concluíram que o microrganismo apresenta capacidade para se instalar e proliferar em terreno previamente sensibilizado, desencadeando o processo oftálmico
Assuntos
Animais , Ceratoconjuntivite Infecciosa/etiologia , Ceratoconjuntivite Infecciosa/prevenção & controle , Neisseria/citologia , Neisseria/isolamento & purificação , Neisseria/patogenicidade , Neisseria/fisiologia , Ovinos , Moraxella bovisRESUMO
Determinaram-se os níveis de resistência à sulfadiazina, clorafenicol, tetraciclina, kanamicina, cefalotina, hetacilina e estreptomicina de 375 amostras de Escherichia coli obtidas de animais. A verificação de resistência infecciosa, nas amostras doadoras, foi feita através de cruzamento com linhagens de Eschrichia coli K12 (K12,712R e K12,J-5F-). A análise dos resultados demonstrou que a maioria das amostras de Escherichia coli comportou-se com resistência simultânea a duas drogas, predominando as amostras resistentes a três drogas. Em todas as espécies animais estudadas, o fator R predominante, em Escherichia coli fez-se representar pelo modelo S-C1-T. Nenhuma das amostras com marcadores para Hetacilina, cefalotina ou kanamicina trasnferiu resistêcia, o mesmo ocorrendo com as amostras monorresistentes para sulfadiazina, clorafenicol e tetraciclina. As amostras com resistência simultânea para duas drogas comportaram-se com competência semelhante à daquelas com três e quatro marcadores de resistência, fazendo exceção o modelo S-C, onde não ocorreu o fenômeno de transferência de resistência (AU).
Assuntos
Animais , Animais , Escherichia coli , Escherichia coli/isolamento & purificaçãoRESUMO
The authors present observations on 20 cases of sporotrichosis, 12 in dogs and 8 in cats, during 18 months of observation. They conclude: 1) In cats and dogs, sporotrichosis is much more frequent than literature indications. 2) Laboratory diagnosis can"t present troubles, since the agent is relatively abundant in the lesions. 3) Evolution of the disease is very slow and it seems not to affect the general conditions of the patients, since it limits it"s action to the cutaneous tissue. 4) Transmission by direct contact seems to be very difficult.
O artigo apresenta resumo em inglês.
RESUMO
Aspergillosis caused by Aspergillus fumigatus was observed in juriti (Leptotilla rufaxilla) and in cacatua (Cacatua moluccensis). These birds were found dead on their cages at the Parque Zoológico, São Paulo Brasil. Necroscopic examination revealed nodular ball shapped formations distributed along the pulmonar parenchyma, air sacks and mesenteric serosa. Pure cultures of Aspergillus fumigatus were obtained on Sabourauds dextrose ágar in both cases, and suspensions of these cultures caused death in rabbits, guinea pigs and pigeons when give by the intra-venous route. The fungs was reisolated from these animals. Addition to water and meal of Lugols solutions was recommended, after thorough desinfection of the cages to prevent spread of infection to susceptibbles. No further cases were observed. The birds refered in this work were not included yet in the list of susceptibles to Aspergillosis in brazilian scientific literature.
O artigo apresenta resumo em inglês.
RESUMO
According to the results of Rajulu & col. (1960) about bacteriphage relations between strains of staphylococci isolated from dogs and men, the A. A. reviewed their data and found that material from cases of furunculosis which they have tested, were many limes related to students, teachers or workers that were in frequent contact with dogs. The cultures from swabs of skin and nostrils of 113 dogs and 42 students revealed that all dogs were carriers of pathogenic staphylococci, on the skin, nostrils or both. Out of 42 students, 10 were carriers. The strains isolated from dogs were all of the albus type and alpha, beta or alpha-beta hemolysin producers. The strains isolated from students were all of the aureus type and only alpha hemolysin producers. All of the strains fermented dextrose but there was no uniformity as to manitol and gelatin. During the work one of the A. A. presented an apicitis of tooth, from which it was obtained, in pure culture, an albus type betahemolytic an coagulase-positive staphylococus (Phage type 29). None of the strains of canine origin could be phage typed utilizing human or bovine series of phages.
O artigo apresenta resumo em inglês.
RESUMO
The method proposed by Robinow & Murray (Scanga, 1959), to demonstrate the cell wall of bacteria is quite seletive for Gram positives but, when applied to Gram negatives, all the cell becomes evenly stained. The method proposed by Knaysi (1941) althought offering a better contrast is of very delicate performance, to be practical. In this paper, the AA. present a new staining method in order to offer a good contrast between the cell wall and cytoplasmic material. Essentially it consists in turning citoplasmic material unstainable by treatment of the cells with a acid mordent. The subsequent stainning with malachite green or Victoria blue gives a very good contrast. The method can be applied either to Gram positive or Gram negative bacteria.
O artigo apresenta resumo em inglês.
RESUMO
The method proposed by Robinow & Murray (Scanga, 1959), to demonstrate the cell wall of bacteria is quite seletive for Gram positives but, when applied to Gram negatives, all the cell becomes evenly stained. The method proposed by Knaysi (1941) althought offering a better contrast is of very delicate performance, to be practical. In this paper, the AA. present a new staining method in order to offer a good contrast between the cell wall and cytoplasmic material. Essentially it consists in turning citoplasmic material unstainable by treatment of the cells with a acid mordent. The subsequent stainning with malachite green or Victoria blue gives a very good contrast. The method can be applied either to Gram positive or Gram negative bacteria.
O artigo apresenta resumo em inglês.
RESUMO
According to the results of Rajulu & col. (1960) about bacteriphage relations between strains of staphylococci isolated from dogs and men, the A. A. reviewed their data and found that material from cases of furunculosis which they have tested, were many limes related to students, teachers or workers that were in frequent contact with dogs. The cultures from swabs of skin and nostrils of 113 dogs and 42 students revealed that all dogs were carriers of pathogenic staphylococci, on the skin, nostrils or both. Out of 42 students, 10 were carriers. The strains isolated from dogs were all of the albus type and alpha, beta or alpha-beta hemolysin producers. The strains isolated from students were all of the aureus type and only alpha hemolysin producers. All of the strains fermented dextrose but there was no uniformity as to manitol and gelatin. During the work one of the A. A. presented an apicitis of tooth, from which it was obtained, in pure culture, an albus type betahemolytic an coagulase-positive staphylococus (Phage type 29). None of the strains of canine origin could be phage typed utilizing human or bovine series of phages.
O artigo apresenta resumo em inglês.
RESUMO
Aspergillosis caused by Aspergillus fumigatus was observed in juriti (Leptotilla rufaxilla) and in cacatua (Cacatua moluccensis). These birds were found dead on their cages at the Parque Zoológico, São Paulo Brasil. Necroscopic examination revealed nodular ball shapped formations distributed along the pulmonar parenchyma, air sacks and mesenteric serosa. Pure cultures of Aspergillus fumigatus were obtained on Sabourauds dextrose ágar in both cases, and suspensions of these cultures caused death in rabbits, guinea pigs and pigeons when give by the intra-venous route. The fungs was reisolated from these animals. Addition to water and meal of Lugols solutions was recommended, after thorough desinfection of the cages to prevent spread of infection to susceptibbles. No further cases were observed. The birds refered in this work were not included yet in the list of susceptibles to Aspergillosis in brazilian scientific literature.
O artigo apresenta resumo em inglês.
RESUMO
The authors present observations on 20 cases of sporotrichosis, 12 in dogs and 8 in cats, during 18 months of observation. They conclude: 1) In cats and dogs, sporotrichosis is much more frequent than literature indications. 2) Laboratory diagnosis can"t present troubles, since the agent is relatively abundant in the lesions. 3) Evolution of the disease is very slow and it seems not to affect the general conditions of the patients, since it limits it"s action to the cutaneous tissue. 4) Transmission by direct contact seems to be very difficult.
O artigo apresenta resumo em inglês.
RESUMO
For the first time in Brazil Sporothricosis is described in dogs. In the two cases studied the disease was characterized by the appearance of cutaneous nodes of different consistences and sometimes ulcerous, covered with dark scabs. Fistulous openings complete the lesion and purulent discharge could be observed as well as involvement of the drainage limph nodes. In the contents of the nodes, in the purulent exudates of the fistulae and the scrapings of the ulcers, there were many parasites, in characteristics "cigar shape". Cultures were obtained easily at room temperature and at 37 C. The disease followed a chronic course and no marked constitucional symptoms were noted. In one of the cases the treatment with Griseofulvina was not successfull but the ministration of potassium iodine resulted in a quick but false recovery, because after some months there was a recrudescency.
Os autores apresentam dois casos de esporotricose em cães, micose ainda não assinalada nessa espécie, em S. Paulo. O processo, em ambos os casos, teve evolução crônica, não afetou aparentemente o estado geral dos animais e caracterizou-se por lesões cutâneas de caráter úlcero-gomoso em diferentes graus de desenvolvimento, configuradas por formações nodulares de consistência diversa, áreas ulceradas e trajetos fistulosos dos quais vazava secreção purulenta. Havia reação linfática bem evidente acusada pelo pronunciado aumento de volume dos gânglios regionais. Em esfregaços irreparados com o pus das fístulas ou cor. raspados das úlceras, corados pelo Gram, o parasita foi facilmente reconhecido pela sua "forma em charuto". Foram obtidas culturas típicas em Sabouraud maltosado e glicosado, em temperatura ambiente e a 37C. Em um dos casos, o tratamento com griseofulvina "(Grifulvin)" não teve êxito. A administração de iodeto de potássio reverteu em rápida melhora, aparentando cura, porém houve recidiva após alguns meses.
RESUMO
For the first time in Brazil Sporothricosis is described in dogs. In the two cases studied the disease was characterized by the appearance of cutaneous nodes of different consistences and sometimes ulcerous, covered with dark scabs. Fistulous openings complete the lesion and purulent discharge could be observed as well as involvement of the drainage limph nodes. In the contents of the nodes, in the purulent exudates of the fistulae and the scrapings of the ulcers, there were many parasites, in characteristics "cigar shape". Cultures were obtained easily at room temperature and at 37 C. The disease followed a chronic course and no marked constitucional symptoms were noted. In one of the cases the treatment with Griseofulvina was not successfull but the ministration of potassium iodine resulted in a quick but false recovery, because after some months there was a recrudescency.
Os autores apresentam dois casos de esporotricose em cães, micose ainda não assinalada nessa espécie, em S. Paulo. O processo, em ambos os casos, teve evolução crônica, não afetou aparentemente o estado geral dos animais e caracterizou-se por lesões cutâneas de caráter úlcero-gomoso em diferentes graus de desenvolvimento, configuradas por formações nodulares de consistência diversa, áreas ulceradas e trajetos fistulosos dos quais vazava secreção purulenta. Havia reação linfática bem evidente acusada pelo pronunciado aumento de volume dos gânglios regionais. Em esfregaços irreparados com o pus das fístulas ou cor. raspados das úlceras, corados pelo Gram, o parasita foi facilmente reconhecido pela sua "forma em charuto". Foram obtidas culturas típicas em Sabouraud maltosado e glicosado, em temperatura ambiente e a 37C. Em um dos casos, o tratamento com griseofulvina "(Grifulvin)" não teve êxito. A administração de iodeto de potássio reverteu em rápida melhora, aparentando cura, porém houve recidiva após alguns meses.