RESUMO
Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.(AU)
Assuntos
Animais , Glucose/análise , Análise do Sêmen , Espermatozoides/citologia , Crioprotetores/análise , Peixes/classificação , CriopreservaçãoRESUMO
Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.
Assuntos
Animais , Análise do Sêmen , Crioprotetores/análise , Espermatozoides/citologia , Glucose/análise , Criopreservação , Peixes/classificaçãoRESUMO
The pharmacokinetics, biodistribution and dosimetry of 99mTc-labeled anti-human epidermal growth factor receptor (anti-hEGF-r) humanized monoclonal antibody (MAb) R3 was investigated following intravenous injection in normal Wistar rats. Serum disappearance curves were best fit by a two-compartment model having a mean distribution half-life (t 1/2alpha) of 0.250 h and a mean elimination (t 1/2beta) of 13.89 h. Among the various organs, a little accumulation of the radiolabeled antibody was found only in kidneys. Biodistribution and dosimetry studies in humans were performed by extrapolation of the animal data to humans. Absorbed dose to normal organs and the remainder of the whole body were estimated using the medical internal radiation dose formula, and dose contributions from radioactivity in transit through the gastrointestinal tract were estimated using a compartment model. Extrapolated values of radiation absorbed dose to normal organs in rads per millicurie administered were whole body, 0.0085; lower large intestine wall, 0.0898; small intestine, 0.0530; upper large intestine wall, 0.0731; and kidneys, 0.0455. The effective dose equivalent predicted was 0.0162 rem/mCi and the effective dose was found to be 0.015 rem/mCi. On the basis of the pharmacokinetics, biodistribution and internal radiation dosimetry information obtained in this study, a diagnostic phase I clinical trial with 99mTc-labeled humanized MAb R3 conjugate in patients should be supported.
Assuntos
Anticorpos Monoclonais/farmacocinética , Receptores ErbB/imunologia , Imunoconjugados/farmacocinética , Compostos de Tecnécio/farmacocinética , Animais , Anticorpos Monoclonais/sangue , Anticorpos Monoclonais/imunologia , Feminino , Humanos , Imunoconjugados/sangue , Imunoconjugados/imunologia , Marcação por Isótopo , Planejamento da Radioterapia Assistida por Computador , Ratos , Ratos Wistar , Compostos de Tecnécio/sangue , Distribuição TecidualAssuntos
Agonistas Adrenérgicos beta/uso terapêutico , Antiasmáticos/uso terapêutico , Asma/tratamento farmacológico , Agonistas Adrenérgicos beta/efeitos adversos , Agonistas Adrenérgicos beta/classificação , Agonistas Adrenérgicos beta/farmacologia , Adulto , Idoso , Antiasmáticos/efeitos adversos , Antiasmáticos/classificação , Antiasmáticos/farmacologia , Arritmias Cardíacas/induzido quimicamente , Asma/mortalidade , Broncodilatadores/farmacologia , Broncodilatadores/uso terapêutico , Criança , HumanosRESUMO
Se pretende evaluar si el Clostridium difficile es un agente productor de diarrea, para lo cual se investigó las heces de 95 niños con diarrea y 26 sin diarrea, atendidos en el Hospital Nacional de Niños "Dr. Carlos Sáenz Herrera", San José, Costa Rica. El estudio tuvo una duración de 4 meses. Simultáneamente, se hizo un estudio sobre otros agentes etiológicos en estas excretas. C. difficile se aisló en un 8.4 por ciento en niños con diarrea y en un 7.7 por ciento en muestras normales. La mayor incidencia fue para Campylobacter fetus sp. jejuni con un 25.8 por ciento en muestras diarreicas y un 3.8 por ciento en niños sin diarrea, seguidamente se ubicó Escherichia coli enteropatógena con 8.4 y 3.8 por ciento en niños con y sin diarrea respectivamente. Se discuten los aspectos clínicos y de laboratorio de los niños que cultivaron C. difficile. Se concluye que puede ser un agente productor de diarrea, pero que hacen falta más estudios sobre este tema
Assuntos
Recém-Nascido , Lactente , Humanos , Masculino , Feminino , Clostridium/isolamento & purificação , Diarreia Infantil/microbiologia , Fezes/microbiologiaRESUMO
Se estudiaron 10 pacientes de sexo masculino, cuyas edades fluctuaban entre los 6 anos y los 13 anos, los que fueron remitidos al Servicio de Neuropsiquiatria Infantil-Adolescente del Area Occidente por conducta afeminada o por otras causas, descubriendose en estos ultimos en el curso de la anamnesis este tipo de sintomatologia. Se hace un analisis de la edad de comienzo de los sintomas, de las caracteristicas de estos (rechazo por juegos y companeros de sexo masculino, preferencia por actividades sedentarias, con ninas o solos, etc.) y de la apariencia externa de estos ninos. Se pone hincapie en la descripcion de ciertas caracteristicas de personalidad en estos pacientes, asi como de su contexto familiar. Se resena brevemente su evolucion y tratamiento. Finalmente, en las conclusiones se recalca la importancia del diagnostico y tratamiento precoz en estos casos, a fin de evitar las alteraciones de la sexualidad adulta, lo que parece altamente probable si se dejan evolucionar a su suerte