RESUMO
INTRODUCTION: The medial collateral ligament (MCL) is one of the main stabilizers of the knee, but its injury occurs in conjunction with other ligaments. OBJECTIVE: To determine the prevalence of MCL lesions by magnetic resonance imaging, their degrees and associated lesions in our institution. MATERIAL AND METHODS: Retrospective study from January to April 2018 where KNEE MRIs were evaluated where the MCL lesion was presented to evaluate the degree and type of associated injuries. RESULTS: We included 368 studies, prevalence of isolated MCL lesion of 3.07%, grade I and grade II, prevalence of concomitant MCL lesions was 17.66% grade I (75%), grade II (15%) and grade III (3%). Associated injuries were medial meniscus injury (46.15%), anterior cruciate ligament injury (30.7%), isolated bone contusion (18.46%), chodral injuries (37.58%), medial vastus injury (14.51%), patellar medial retinacular injury (14.51%), vastus lateral injury (9.23%), posterior cruciate ligament injury (6.15%), lateral meniscus injury (4.61%), iliotibial band tenosynovitis (4.61%), medial facet avulsion fracture (3.07%), Pes Anserine tenosynovitis (3.07%). CONCLUSION: Prevalence of 17.66% of the MCL injuries in our hospital by magnetic resonance, the first 2 degrees predominate, with a wide spectrum of associated knee injuries.
INTRODUCCIÓN: El ligamento colateral medial (LCM) es uno de los principales estabilizadores de la rodilla, pero su lesión se presenta en conjunto con otras lesiones ligamentarias. OBJETIVO: Determinar la prevalencia de lesiones del LCM por resonancia magnética, sus grados y lesiones asociadas en nuestra institución. MATERIAL Y MÉTODOS: Estudio retrospectivo de Enero a Abril de 2018, se evaluaron resonancias magnéticas de rodilla donde se presentó lesión del LCM para evaluar grado y tipo de lesiones asociadas. RESULTADOS: Se incluyeron 368 estudios, prevalencia de lesión aislada del LCM de 3.07%, una grado I y una grado II, la prevalencia de lesiones de LCM concomitantes fue de 17.66%, grado I (75%), grado II (15%) y grado III (3%). Las lesiones asociadas fueron lesión del menisco medial (46.15%), lesión del ligamento cruzado anterior (30.7%), contusión ósea aislada (18.46%), lesiones condrales (37.58%), lesión de vasto medial (14.51%), lesión del retináculo medial patelar (14.51%), lesión del vasto lateral (9.23%), lesión del ligamento cruzado posterior (6.15%), lesión del menisco lateral (4.61%), tenosinovitis banda iliotibial (4.61%), fractura de avulsión de la faceta medial (3.07%), tenosinovitis de la Pes Anserinus (3.07%). CONCLUSIÓN: Prevalencia de 17.66% de lesiones del LCM en nuestro hospital por resonancia magnética, predominan los dos primeros grados con un espectro amplio de lesiones asociadas de la rodilla.
Assuntos
Ligamentos Colaterais , Imageamento por Ressonância Magnética , Ligamentos Colaterais/diagnóstico por imagem , Humanos , Espectroscopia de Ressonância Magnética , Prevalência , Estudos RetrospectivosRESUMO
One of the main challenges in the food industry is to design strategies for the successful incorporation of natural sources of bioactive compounds. Recently, yogurts and other fermented dairy beverages have been proposed as ideal carriers of such bioactive compounds such as fatty acids and antioxidants that could improve consumers' health. However, the incorporation of new ingredients causes functional and structural modifications that may affect the consumers' preferences. In this work, a dairy beverage model supplemented with oleic acid has been designed by partial substitution of milk by Candida utilis single-cell protein extract. The changes in the structural properties of this new beverage were evaluated by following the fermentation process, pH, aggregate size, microstructure, and changes in rheological properties. Furthermore, molecular dynamics simulations were carried out to analyze the interaction between its main components. Our data revealed that samples with a percentage of milk substitution of 30% showed a higher viscosity as compared with the other percentages and less viscosity than the control (no substitution). These samples were then selected for fortification by incorporating oleic acid microcapsules. A concentration of 1.5 g/100 g was shown to be the optimal quantity of microcapsules for oleic acid supplementation. Molecular dynamic simulations revealed glutathione as an important component of the micro-gel structure. The present study forms the basis for novel studies where Candida utilis single-cell protein and microencapsulated essential oils could be used to design innovative bioproducts.
Assuntos
Bebidas/análise , Candida/química , Proteínas Alimentares/química , Ácido Oleico/química , Animais , Antioxidantes/análise , Suplementos Nutricionais , Ácidos Graxos/análise , Fermentação , Glutationa/metabolismo , Concentração de Íons de Hidrogênio , Leite/química , Óleos Voláteis/química , Reologia , ViscosidadeRESUMO
La diabetes es un trastorno metabólico crónico de gran alcance epidemiológico. Numerosos estudios han demostrado que la diabetes mellitus se encuentra asociada al incremento de la formación de radicales libres derivados del oxígeno y a la disminución del potencial antioxidante del organismo, generando el daño oxidativo de algunos componentes de la célula tales como las proteínas, carbohidratos, lípidos y ácidos nucleicos. Este trabajo pretente establecer, en ratas Wistar, la relación dosis-respuesta de estreptozotocina frente a los niveles de glucosa y a diferentes parámetros del estatus antioxidante. Como marcadores del daño asociado a las especies de oxígeno reactivas se evaluó la actividad enzimática de catalasa y de superóxido dismutasa, la capacidad antioxidante total del plasma y la peroxidación lipídica. Los resultados obtenidos evidencian una clara relación de la dosis de estreptozotocina con los niveles de glucosa y los parámetros de estrés oxidativo evaluados luego de 20 días de la inducción de la diabetes experimental.
Assuntos
Diabetes Mellitus Experimental , Estresse OxidativoRESUMO
The ability of cupric ions to immobilize human spermatozoa was assessed in vitro using an optical microscope. A Ringer's solution containing human spermatozoa and three cupric ion concentrations of 8 x 10(-8) mol/L, 8 x 10(-6) mol/L and 8 x 10(-5) mol/L were tested. The higher cupric ion concentrations (8 x 10(-5) mol/L and 8 x 10(-6) mol/L) significantly reduced spermatozoa motility after about 20 min. However, with the lower cupric ion concentration tested (8 x 10(-8) mol/L), also for 20-min experimentation, the reduction in motility was more limited.
Assuntos
Cobre/administração & dosagem , Soluções Isotônicas/administração & dosagem , Motilidade dos Espermatozoides/efeitos dos fármacos , Cobre/análise , Relação Dose-Resposta a Droga , Humanos , Concentração de Íons de Hidrogênio , Soluções Isotônicas/química , Cinética , Masculino , Solução de Ringer , Contagem de EspermatozoidesRESUMO
Histidine-rich proteins have been associated with Plasmodium falciparum infected red blood cells (RBC) cytoadherence, and RBC rosetting; these phenomena may cause clogging of the post-capillary venules, this being one of the main causes of severe cerebral malaria. They may also participate in parasite mature stages' evasion of the immune system and their subsequent destruction in the spleen. Non-overlapping synthetic peptides, corresponding to entire amino acid sequences reported for the KAHRP-I, HRP-II and HRP-III proteins, were used in RBC binding assays. Peptides with high and low binding activity were recognized. The KAHRP-I protein shows 3 peptides with high binding affinity to RBCs, two of them variable (peptide 6783, sequence 321QNYVHPWSGYSAPYGVPHGA(340) and peptide 6789, sequence 441KKREKSIMEKNHAAKKLTKK(460)) and the other conserved (peptide 6786, sequence 381KSKKHKDHDGEKKKSKKHKD(400)) having affinity constant of around 190 nM and 1000 binding sites per cell. Interestingly, this peptide shares aminoacid sequences with one reported as being recognized by malaria exposed human antibodies. The HRP-I protein also presents one conserved peptide (peptide 6800, sequence 24NNSAFNNNLCSKNAKGLNLN(43)) with high affinity, located in the amino terminal region of the native protein, having 210 nM affinity constant and 6000 receptor sites. The HRP-III protein only contains peptides with low binding activity. Treatment of red blood cells with neuraminidase reduces the binding of the conserved high binding 6786 and 6800 peptides. Anti-glycophorins A, B and C antibodies inhibit the binding of the conserved high binding 6786 and 6800 peptides. Furthermore, the specific determination of glycoproteins by chemioluminescenoe, in SDS/PAGE western blot, suggests that these glycophorins could be the receptor for these high binding peptides. High binding peptides' critical amino acids, involved in RBC binding were determined by means of competition binding assays.
Assuntos
Eritrócitos/parasitologia , Fragmentos de Peptídeos/metabolismo , Plasmodium falciparum/metabolismo , Proteínas de Protozoários/metabolismo , Sequência de Aminoácidos , Animais , Anticorpos/farmacologia , Ligação Competitiva/efeitos dos fármacos , Quimotripsina , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Glicoforinas/imunologia , Humanos , Neuraminidase , Fragmentos de Peptídeos/antagonistas & inibidores , Fragmentos de Peptídeos/síntese química , Peptídeos/química , Ligação Proteica , Proteínas/química , Proteínas de Protozoários/química , TripsinaAssuntos
Polirradiculoneuropatia/diagnóstico , Polirradiculoneuropatia/fisiopatologia , Polirradiculoneuropatia/classificação , Nervos Periféricos/patologia , Reflexo Anormal , Eletromiografia , Diagnóstico Diferencial , Doenças do Sistema Nervoso Periférico/fisiopatologia , Doenças do Sistema Nervoso Periférico/complicaçõesAssuntos
Polirradiculoneuropatia/diagnóstico , Polirradiculoneuropatia/classificação , Polirradiculoneuropatia/fisiopatologia , Reflexo Anormal , Doenças do Sistema Nervoso Periférico/complicações , Doenças do Sistema Nervoso Periférico/fisiopatologia , Diagnóstico Diferencial , Eletromiografia , Nervos Periféricos/patologiaRESUMO
Estudio prospectivo realizado en el Hospital General de Medellin Colombia, con 1.300 recien nacidos consecutivamente, por medio de un protocolo que contenia los datos de la Historia Clinica materna y perinatal. Se encontro que 10.46% de estos recien nacidos, fueron de bajo peso, y que entre los factores de riesgo materno que fueron mas significativos para el BPN estan la patologia materna durante el embarazo, con el mayor porcentaje para la ruptura prematura de membranas 22.58%. La prematurez fue el factor de riesgo mas significativo entre los fetales, con 50.7% de R.N. pretermino con BPN. La mortalidad perinatal de los R.N. con BPN estudiados, donde fue de solo 1.61%. Podemos concluir que los factores mencionados juegan un papel fundamental en el resultado final madre-recien nacido, y que la incidencia de BPN y la morbimortalidad perinatal no ha variado en los ultimos anos en Latinoamerica
Assuntos
Humanos , Masculino , Feminino , Recém-Nascido de Baixo Peso , Fatores de Risco , Colômbia , Mortalidade/tendênciasRESUMO
Se prepararon los reactivos para la determinacion de amoniaco plasmatico. La resina empleada es una resina cationica fuertemente acida (amberlita 252 de la casa Rohm and Haas) y como reaccion de color se empleo la reaccion de Berthelot. Se correlacionaron los de una casa comercial obteniendose una correlacion de 0,93 con una recuperacion de 97, 104, 76 por ciento para valores bajos, intermedios y altos respectivamente y cumpliendose la ley de Beer y Lambert en nuestras condiciones de trabajo hasta 400 microgramas por cien mililitros