RESUMO
The purpose of the survey was to determine acute & chronic toxicity; in vivo antioxidant and anti-inflammatory actions of the different extracts of A. fraxinifolius Wight and Arn bark; along with estimation of the phenolic, flavonoidal contents and investigation of phenolic metabolites that may attribute to the activities. LD50 of the total ethanol extract (TEE) was 7.1 g/kg b. wt, the radical scavenging activity of DPPH showed 60.31% inhibition, FRAP ability and ABTS+ activity showed 55.024 and 67.217 µmol Trolox/100 g dry weight, respectively. TEE followed by ethyl acetate extract (EAE) at 100 mg/kg b.w exhibited the highest in vivo antioxidant activity (94.51% and 91.08% potency, respectively) compared with Vit E (100%). The TEE & EAE exhibited the highest anti-inflammatory activity (3.81±0.08 & 3.79±.0.04) respectively in comparison with indomethacin 3.83±0.01 measured as edema diameter after 4 hours of extract administration. The total phenolic and total flavonoid contents in the total ethanol extract (TEE) estimated as gallic acid and catechin equivalents were 61.06± 0.08 µg eq GA/g, 40.33± 0.20 µg CE/g extract respectively. EAE revealed five phenolic acids and eight flavonoid compounds isolated for the first time from the plant
Assuntos
/análise , Fabaceae/toxicidade , Antioxidantes/análise , Ácidos/efeitos adversos , Etanol , Dose Letal Mediana , Acetatos/administração & dosagemRESUMO
The small ruminant industry has been identified by Caribbean governments as key to agricultural development and food security. ⢠The economic impact of these prolific species is significant to farmers both locally and regionally, given its contribution to foreign exchange, employment generation and poverty alleviation ⢠Recognizing disease is critical for a small ruminant based economy to possess both sustainability and viability. Understanding ways to accurately identify, prevent, cure and control disease is necessary to improve the economic status of this industry. ⢠Post mortem evaluation is a useful diagnostic tool as it can reliably confirm, refute or augment ante-mortem diagnoses. ⢠Respiratory disease is of particular importance as it is commonly encountered in small ruminant flocks, affecting groups or individuals of any breed, age and sex. More often than not, it involves a combination of infectious and non-infectious aetiologies. An interstitial, bronchinterstital or bronchopneumonia may develop which is largely dependent upon the causative agent.