RESUMO

We examined envelope protein profiles, chromosomal restriction endonuclease digest patterns, and immune responses to envelope proteins for collections of Salmonella typhi strains isolated in Peru and Indonesia. Only minor differences in envelope protein patterns were apparent among strains. Strains from 7 of 20 Indonesian patients had a distinct chromosomal digest pattern compared with patterns of Peruvian and other Indonesian strains. Strains with this pattern carried the gene for the j flagellar antigen (H1-j); differences in response to envelope proteins of j and d strains were noted on immunoblot analysis. Our data suggest that there are genotypic and phenotypic differences among S. typhi strains. The clinical importance of these differences remains to be fully evaluated; however, in this study it was not possible to show a clear correlation between strain characteristics and disease severity.

Assuntos

Salmonella typhi/classificação , Proteínas de Bactérias/isolamento & purificação , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Flagelos , Genes Bacterianos , Humanos , Immunoblotting , Indonésia , Peru , Reação em Cadeia da Polimerase , Salmonella typhi/genética , Salmonella typhi/isolamento & purificação , Febre Tifoide/imunologia , Febre Tifoide/microbiologia

Assuntos

DNA Bacteriano/análise , Salmonella typhi/isolamento & purificação , Antígenos Virais/genética , Colódio , DNA Recombinante , Reações Falso-Negativas , Filtração/instrumentação , Bactérias Gram-Negativas/genética , Humanos , Indonésia , Hibridização de Ácido Nucleico , Peru , Salmonella typhi/genética , Febre Tifoide/microbiologia