RESUMO
Because estimates of the biotin requirement for infants currently are based on the biotin concentration in human milk, we sought to determine whether inactive biotin metabolites are present. Samples were collected for 7 weeks post partum from 15 healthy women. Biotin and the inactive metabolites bisnorbiotin and biotin sulfoxide were measured by means of a high-performance liquid chromatography avidin-binding assay. At 8 days post partum the proportion of biotin was 44%, bisnorbiotin 48%, and biotin sulfoxide 8%. Although biotin content increased post partum (p < 0.003), the metabolites remained an important portion of the total providing evidence that accurate measurement of biotin in human milk requires an assay that is specific for biotin.
Assuntos
Biotina/análise , Leite Humano/química , Adulto , Biotina/análogos & derivados , Biotina/metabolismo , Cromatografia Líquida de Alta Pressão , Feminino , Humanos , Fenômenos Fisiológicos da Nutrição do Lactente , Recém-Nascido , Necessidades Nutricionais , Período Pós-Parto , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Fatores de TempoAssuntos
Aleitamento Materno , Comparação Transcultural , Atenção à Saúde , Adolescente , Adulto , Bolívia , Feminino , Honduras , Humanos , Lactente , Recém-Nascido , Centros de Saúde Materno-Infantil , Filipinas , Fatores Socioeconômicos , DesmameRESUMO
PIP: Infant feeding issues and research commonly portray infant feeding practices as a dichotomous variable; i.e., breastfeeding vs. bottlefeeding. This research establishes the complexity and variability in infant feeding patterns in 3 sites in the developing world. Comparative data are presented on the modes and products used to feed infants in Kinshasa, Zaire; St. Kitts-Nevis, West indies; and Cebu City, Philippines. They demonstrate that in these areas most infants receive some breastmilk. Bottlefeeding is also common but is rarely the exclusive mode of feeding. And the prevalence, manner of use, and role of the bottle varies among the 3 sites. Early supplementation of breastmilk by a variety of liquids, semisolids, and solids is typical of all 3 areas. These findings have important implications for educators and policymakers, as well as for research efforts relating infant feeding practices to infant health outcomes.^ieng