RESUMO
We report the nucleotide sequence and genetic diversity of part of the envelope (env) gene of four strains of feline immunodeficiency virus (FIV) isolated from Argentine domestic cats. The DNA encoding the V3 to V5 regions of the env gene of the FIV isolates were amplified by PCR, cloned and sequenced. Phylogenetic analysis revealed that the Argentine isolates did not cluster into a single group; one isolate clustered with subtype B FIV isolated in the USA and Japan, whereas the others formed a new cluster of FIV which might represent a prototype sequence for subtype E.
Assuntos
Genes env , Variação Genética , Vírus da Imunodeficiência Felina/genética , Sequência de Aminoácidos , Animais , Argentina , Sequência de Bases , Gatos , Vírus da Imunodeficiência Felina/classificação , Vírus da Imunodeficiência Felina/isolamento & purificação , Dados de Sequência Molecular , Filogenia , Homologia de Sequência de AminoácidosRESUMO
The nucleotide sequences of the long terminal repeat of five Japanese, five Argentine and three Australian isolates of feline immunodeficiency virus (FIV) were determined and compared with those of isolates previously described. The results revealed that the Japanese isolates were found to cluster with nucleotide sequence similarity of 95.6%-99.4%. The Australian isolates also clustered with nucleotide sequence similarity of 97.2%-99.4%. The Argentine isolates formed two groups; the LP9 isolate is closely related to the Japanese isolates, whereas the LP1, LP3, LP20 and LP24 isolates are distant from both the Japanese and Australian isolates. From these results, FIV can be divided into three groups, namely: (I) the Californian, Australian and British isolates; (II) the Japanese isolates and one Argentine LP9 isolate; (III) the other Argentine isolates.