RESUMO
PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod immunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression.
Assuntos
Queimaduras/radioterapia , Terapia com Luz de Baixa Intensidade/métodos , Músculo Esquelético/efeitos da radiação , Proteína MyoD/análise , Animais , Queimaduras/metabolismo , Queimaduras/patologia , Contagem de Células , Modelos Animais de Doenças , Imuno-Histoquímica , Masculino , Fibras Musculares Esqueléticas/efeitos da radiação , Músculo Esquelético/patologia , Proteína MyoD/efeitos da radiação , Ratos Wistar , Reprodutibilidade dos Testes , Pele/lesões , Pele/efeitos da radiação , Fatores de Tempo , Resultado do TratamentoRESUMO
PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression. .
Assuntos
Animais , Masculino , Queimaduras/radioterapia , Terapia com Luz de Baixa Intensidade/métodos , Músculo Esquelético/efeitos da radiação , Proteína MyoD/análise , Queimaduras/metabolismo , Queimaduras/patologia , Contagem de Células , Modelos Animais de Doenças , Imuno-Histoquímica , Fibras Musculares Esqueléticas/efeitos da radiação , Músculo Esquelético/patologia , Proteína MyoD/efeitos da radiação , Ratos Wistar , Reprodutibilidade dos Testes , Pele/lesões , Pele/efeitos da radiação , Fatores de Tempo , Resultado do TratamentoRESUMO
PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression.(AU)