RESUMO
Background: A novel generation of immunohistochemical visualization systems based on a biotin-free polymeric (BFP) technology has recently been released. We have compared the new BFP and the classical streptavidin-biotin (SAB) systems to evaluate estrogen receptor in breast carcinomas. Methods: Serial sections from a tissue microarray containing 320 invasive breast carcinomas were stained by immunohistochemistry for estrogen receptor using the rabbit monoclonal antibody SP1. Eleven different visualization systems were used, including seven BFP systems (six second-generation: DAKO Advance TM , Leica Novolink TM, Zymed SuperPicTureTM , Zymed PicTure Max TM , Biogenex Super Sensitive Non-Biotin HRP TM , CellMarque Mouse/Rabbit Polydetector HRP/DABTM ; one first-generation: DAKO EnVision+TM) and four SAB systems (DAKO LSAB+TM ; Signet EasyPathTM ; Biogenex Super SensitiveTM and CellMarque Mouse/Rabbit Immunodetector HRP/DABTM). All visualization systems were used following the instructions provided by the manufacturers. All slides were scanned using Zeiss Mirax Scan, and the intensity of immunohistochemistry staining was automatically quantified using HistoQuant software. The cytoplasm staining was visually evaluated as absent (0), weak (1), moderate (2), or strong (3). Results: The BFP Advance and Novolink , and the SAB LSAB + showed the highest staining intensity among all the systems (P<0.01). However, LSAB+ showed the highest cytoplasm staining among those used (p<0.01). .. The BFP Advance and Novolink showed the strongest staining intensity and, followed by all the other second-generation BFPs, represent a powerful tool for immunohistochemistry standardization of estrogen receptor evaluation of breast carcinomas.
Assuntos
Biotina , Neoplasias da Mama , Análise em Microsséries , Receptores de Estrogênio , Neoplasias da Mama/diagnósticoRESUMO
In the last few years, telepathology has benefited from the progress in the technology of image digitalization and transmission through the world web. The applications of telepathology and virtual imaging are more current in research and morphology teaching. In surgical pathology daily practice, this technology still has limits and is more often used for case consultation. In the present review, we intend to discuss its applications and challenges for pathologists and scientists. Much of the limitations of virtual imaging for the surgical pathologist reside in the capacity of storage of images, which so far has hindered the more widespread use of this technology. Overcoming this major drawback may revolutionize the surgical pathologist's activity and slide storing.
Assuntos
Patologia Cirúrgica/métodos , Consulta Remota/métodos , Telepatologia/métodos , Instrução por Computador/instrumentação , Instrução por Computador/métodos , Humanos , Processamento de Imagem Assistida por Computador/métodos , Internet/instrumentação , Patologia Cirúrgica/educação , Patologia Cirúrgica/instrumentação , Controle de Qualidade , Consulta Remota/instrumentação , Software , Telepatologia/instrumentação , Interface Usuário-ComputadorRESUMO
Este no es un libro sobre religión, sino mas bien una obra que pertenece a la catgoría de testimonio. Keith Miller no discute primordialmente el testimonio; proporciona en cambio un testimonio de primera mano de lo que hasta ahoraha acontecido en su propia vida. El libro es un informe provisional de la manera en que Dios se ha llegado a él en el dolor y en la alegría