RESUMO
Aim. To evaluate the osteogenic potential of chalcones using the rat critical size calvarial defect. Methods. The chalcones were synthesized from acetophenone following the Claisen-Schmidt aldol condensation method by varying the substituted benzaldehydes (3,4-Cl; 4-Cl; 4-CH3; 4-OCH3, H). The five chalcone molecules were evaluated in three concentrations (1 percent, 5 percent and 10 percent) in comparison to control and vehicle (Vaseline) groups. The results of the remaining wound areas were calculated statistically by the ANOVA method followed by the Student-Newman-Keuls test and the histological sections were analyzed qualitatively by light microscopy. Results. All molecules at 10 percent concentration showed significant bone closure compared to the control, vehicle and chalcone groups at 1 percent concentration (p<0.01). Active osteoblasts were observed on the repair surfaces in all groups treated with chalcones. Treatment with the C5 molecule at concentration of a 10 percent resulted in greater bone neoformation compared to the other molecules, with features of secondary bone observed. Conclusion. The chalcones evidenced a dose-dependent osteogenic potential and C5 was more effective in bone repair
Assuntos
Animais , Feminino , Ratos , Osteogênese , Chalconas/síntese química , Ratos WistarRESUMO
BACKGROUND: This study describes the bone formation stimulated by the application of a type of chalcone to critical-size defects in rat calvarial bone. MATERIAL AND METHODS: Sixty female Wistar rats were divided into 6 groups of 10 animals per group: control (no treatment), vehicle (vaseline) and the chalcone (1-phenyl-3-(4-chlorophenyl)-2-propen-1-one) suspended in vaseline at 10%. A critical-size defect of 5 mm was prepared using a trephine in the calvarial bone, after which the treatment was applied, in a single dose, according to the experimental group. The samples were evaluated macroscopically using ImageJ software, and histologically 30 and 45 days after surgery. RESULTS: At 30 days after surgery, there was significant bone formation (p < 0.05) in the groups treated with chalcone, compared with the other groups. Many active osteoblasts were observed adjacent to the borders of the newly formed bone tissue. 45 days after surgery in the chalcone group, the surgical defects showed complete bone closure. CONCLUSION: The results of this study suggest that chalcone has significant potential to induce the formation of new bone.
Assuntos
Doenças Ósseas/tratamento farmacológico , Chalcona/uso terapêutico , Chalconas/uso terapêutico , Osteogênese/efeitos dos fármacos , Crânio/efeitos dos fármacos , Acetofenonas/química , Animais , Benzaldeídos/química , Matriz Óssea/efeitos dos fármacos , Matriz Óssea/patologia , Calcificação Fisiológica/efeitos dos fármacos , Chalcona/síntese química , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/patologia , Feminino , Osteoblastos/efeitos dos fármacos , Osteoblastos/patologia , Osteócitos/efeitos dos fármacos , Osteócitos/patologia , Vaselina , Veículos Farmacêuticos , Ratos , Ratos Wistar , Crânio/patologia , Hidróxido de Sódio/química , Fatores de TempoRESUMO
A adequação do ambiente físico quanto aos hábitos da espécie em relação ao bem-estar animal e o refinamento das pesquisas, ultimamente vem ganhando importância investigativa. Com o objetivo de avaliar o comportamento de ratos alojados em caixas de diferentes cores, utilizaram-se 48 animais machos com 3 meses de idade divididos em seis grupos, sendo que três grupos foram alojados em caixas brancas e outros três em caixas pretas, durante 4 semanas. Empregando-se o labirinto em cruz elevado (LCE), os resultados mostraram maior tempo de permanência nos braços fechados dos animais alojados nas caixas brancas. No teste do campo aberto (CA), houve maior ambulação do grupo mantido em caixas pretas, e na esquiva inibitória, os dados indicaram um aumento significativo no tempo de permanência na plataforma do grupo alojado em caixas pretas. Assim, os animais alojados em gaiolas de cor preta apresentaram maior tendência exploratória (menor ansiedade) e melhor aprendizado.
Lately, the adequacy of a physical ambient to the species habits relating to their well being and refined researches is getting important for researchers. With the aim of evaluate the behavior of rats housed in boxes of different colors, it was used 48 male animals, with 3 months of age, divided in 6 groups, three groups in white boxes and the other three in black boxes, for 4 weeks. Observing on the elevated plus maze, the results showed that the animals from white boxes stayed more time in the closed arms. On the open field test, there was more walking of the group kept in Black boxes, and on the inhibitory avoidance, data indicated a significant increase on the time spent on the platform by the group housed in the black boxes. Thus, the animal housed in Black cages have shown a greater exploratory tendency (decreased anxiety), and better learning.
Assuntos
Animais , Animais de Laboratório , Comportamento Exploratório , Ratos WistarRESUMO
A adequação do ambiente físico quanto aos hábitos da espécie em relação ao bem-estar animal e o refinamento das pesquisas, ultimamente vem ganhando importância investigativa. Com o objetivo de avaliar o comportamento de ratos alojados em caixas de diferentes cores, utilizaram-se 48 animais machos com 3 meses de idade divididos em seis grupos, sendo que três grupos foram alojados em caixas brancas e outros três em caixas pretas, durante 4 semanas. Empregando-se o labirinto em cruz elevado (LCE), os resultados mostraram maior tempo de permanência nos braços fechados dos animais alojados nas caixas brancas. No teste do campo aberto (CA), houve maior ambulação do grupo mantido em caixas pretas, e na esquiva inibitória, os dados indicaram um aumento significativo no tempo de permanência na plataforma do grupo alojado em caixas pretas. Assim, os animais alojados em gaiolas de cor preta apresentaram maior tendência exploratória (menor ansiedade) e melhor aprendizado.(AU)
Lately, the adequacy of a physical ambient to the species habits relating to their well being and refined researches is getting important for researchers. With the aim of evaluate the behavior of rats housed in boxes of different colors, it was used 48 male animals, with 3 months of age, divided in 6 groups, three groups in white boxes and the other three in black boxes, for 4 weeks. Observing on the elevated plus maze, the results showed that the animals from white boxes stayed more time in the closed arms. On the open field test, there was more walking of the group kept in Black boxes, and on the inhibitory avoidance, data indicated a significant increase on the time spent on the platform by the group housed in the black boxes. Thus, the animal housed in Black cages have shown a greater exploratory tendency (decreased anxiety), and better learning.(AU)
Assuntos
Animais , Ratos Wistar , Comportamento Exploratório , Animais de LaboratórioRESUMO
The main goal of this study was the analysis of the developmental potentiality of tooth germ from late bell stage on, after its heterotopic placement within the skin. Teeth germs of newborn rats were grafted within a skin pouch of the ear of adult rats. Seven to fourteen days after grafting, dental germs developed normal dental structures in which ameloblasts and odontoblasts were well differentiated. Twenty to forty-one days after graft, the inflammatory host reaction destroyed the dental developed tissues by cell infiltration. The dentin of the grafts was of osteoid characteristics, and its size increased depending on grafting time until the complete substitution of all dental tissues. This atypical dentin showed several degrees of polymerisation from collagen fibres smooth dentin devoid near the graft a to fibres rich dentin far from the dental germ. Present results suggest that this type of dental graft could be a valuable model to study the self-development of dental tissues and the reactive mechanisms taking place after dental injuries.