RESUMO
Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve animals were seropositive to ELISA and eleven animals were positive by RT-PCR. This is the first report of EHDV nucleic acid detection in WTD from Mexico. It is hypothesized that applying the transboundary disease approach to interdisciplinary research will help fill knowledge gaps, which could help develop countermeasures to mitigate the threat of EHDV infection in wildlife and livestock along the U.S.-Mexico border.(AU)
Virus da doença hemorrágica epizoótica (EHDV) são arbovírus dsRNA transmitidos por mordidas do genus Culicoides que causam doenças em ruminantes domésticos e selvagens. Doença hemorrágica epizoótica (EHD) é considerada uma das doenças infecciosas mais importantes dos veados de cauda branca (WTD) na América do Norte. Alguns estudos no Nordeste do México relatam soropositividade para EHDV em WTD e vetores Culicoides infectados com EHDV. A crescente população de WTD que compartilham hábitats com pecuária no Nordeste do México realçam a importância de EHD para a indústria pecuária na região de fronteira com os Estados Unidos. Cento e vinte duas amostras de WTD no estado de Tamaulipas, Mexico, foram testados por ELISA e RT-PCR para anticorpos e ácido nucleico de EHDV, respectivamente. Esse é o primeiro relato de detecção de ácido nucleico de EHDV em WTD do México. A hipótese é de que a aplicação de uma resposta transfronteira e pesquisa interdisciplinar ajudará a preencher lacunas de conhecimento levando a medidas reativas para mitigar a ameaça de infecção por EHDV na pecuária e animais selvagens na fronteira entre os Estados Unidos e o Mexico.(AU)
Assuntos
Animais , Cervos/genética , Testes Sorológicos/veterinária , Vírus da Doença Hemorrágica EpizoóticaRESUMO
Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve animals were seropositive to ELISA and eleven animals were positive by RT-PCR. This is the first report of EHDV nucleic acid detection in WTD from Mexico. It is hypothesized that applying the transboundary disease approach to interdisciplinary research will help fill knowledge gaps, which could help develop countermeasures to mitigate the threat of EHDV infection in wildlife and livestock along the U.S.-Mexico border.(AU)
Virus da doença hemorrágica epizoótica (EHDV) são arbovírus dsRNA transmitidos por mordidas do genus Culicoides que causam doenças em ruminantes domésticos e selvagens. Doença hemorrágica epizoótica (EHD) é considerada uma das doenças infecciosas mais importantes dos veados de cauda branca (WTD) na América do Norte. Alguns estudos no Nordeste do México relatam soropositividade para EHDV em WTD e vetores Culicoides infectados com EHDV. A crescente população de WTD que compartilham hábitats com pecuária no Nordeste do México realçam a importância de EHD para a indústria pecuária na região de fronteira com os Estados Unidos. Cento e vinte duas amostras de WTD no estado de Tamaulipas, Mexico, foram testados por ELISA e RT-PCR para anticorpos e ácido nucleico de EHDV, respectivamente. Esse é o primeiro relato de detecção de ácido nucleico de EHDV em WTD do México. A hipótese é de que a aplicação de uma resposta transfronteira e pesquisa interdisciplinar ajudará a preencher lacunas de conhecimento levando a medidas reativas para mitigar a ameaça de infecção por EHDV na pecuária e animais selvagens na fronteira entre os Estados Unidos e o Mexico.(AU)
Assuntos
Animais , Cervos/genética , Testes Sorológicos/veterinária , Vírus da Doença Hemorrágica EpizoóticaRESUMO
Searching for bacterial probiotics active upon Helicobacter pylori continue to be an important clinical challenge because of the increased prevalence of this highly priority pathogen in humans. In this work, we assess the in vivo anti-H. pylori SS1 (cagA+/vacAs2m2+) properties of a previously isolated human gastric probiotic strain Lactobacillus fermentum UCO-979C by using a Meriones unguiculatus (Mongolian gerbil) model. Animals were administered with a saline suspension of L. fermentum UCO-979C or H. pylori SS1 as negative and positive control for H. pylori colonisation controls, prior to assayed the challenge group that was administered with these two species per animal for detecting protective activity of the probiotic strain against colonisation. The results showed that L. fermentum UCO-979C strongly inhibited the colonisation of H. pylori decreasing up to 87% of the colonisation in the antrum by the pathogen, suggesting that this probiotic strain has a strong probiotic activity against H. pylori in the most valuable animal model for in vivo assays nowadays.
Assuntos
Infecções por Helicobacter/prevenção & controle , Helicobacter pylori/efeitos dos fármacos , Limosilactobacillus fermentum , Probióticos/farmacologia , Animais , Modelos Animais de Doenças , Gerbillinae , Humanos , Probióticos/administração & dosagem , Estômago/microbiologiaRESUMO
Changes in starch characteristics caused by alkaline-cooking in two corn genotypes (hard and soft) were studied in the present work. Color, pH, pasting properties (Brabender viscoamylograph), water sorption, solubility, swelling power and thermal analysis (Differential scanning calorimetry) were determined in starches previously extracted, from raw and alkaline-cooked hard and soft corns. A reduction in maximum viscosity peak, temperature at the endothermic peak and swelling power at 90 degrees C, and an increase in solubility was observed in starches obtained from both hard and soft corn after alkaline-cooking. Starch from hard corn showed an increment in initial pasting temperature after alkaline-cooking. Alkaline-cooking induced higher modifications in hard than in soft corn starch, probably as a result of its endosperm type and grain hardness, although optimum cooking times were used for each corn genotype.
Assuntos
Temperatura Alta , Amido/química , Zea mays/química , Grão Comestível/químicaRESUMO
BACKGROUND: Fecundity rates have decreased considerably in Latin America, due to a higher contraceptive use and better family planning programs. AIM: To determine whether neighborhood level socioeconomic variables have an independent effect on reported modern contraceptive use, over and above the effect of individual level measures of socioeconomic status and reproductive health behavior. SUBJECTS AND METHODS: Multilevel logistic models determined the effects of individual and neighborhood dimensions (socioeconomic status, urbanization, quality of public health facilities) on contraceptive use among 509 women aged 15 to 49 years living in 85 neighborhoods within the Region of Bio Bio, Chile. RESULTS: After adjustment for women's individual socioeconomic characteristics and other risk factors, less favorable neighborhood socioeconomic characteristics were significantly associated with lower rates of modern contraceptive use. CONCLUSIONS: Changes in the quality of facilities for family planning at the neighborhood level may enhance modern contraceptive use.
Assuntos
Humanos , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Classe Social , Comportamento Contraceptivo/estatística & dados numéricos , Qualidade da Assistência à Saúde , Chile , Fatores Socioeconômicos , Instalações de Saúde , Modelos Logísticos , UrbanizaçãoRESUMO
The ingestion of iron-containing products is a potential toxicological emergency. It is a leading cause of pediatric unintentional ingestion fatalities because some iron-containing compounds are readily available (vitamin mixtures, iron pills). Among adults, it has been described as accidental overdoses (mainly in pregnancy) and as suicidal attempts. We report a 42 years old woman, admitted due to the intentional ingestion of iron pills with suicidal purposes. A plain abdominal X ray showed at least 20 pills in the gastric fundus and antrum. She was successfully treated with intravenous desferroxamine chelation. Plain abdominal X ray examination can be very useful in the diagnosis of acute iron poisoning.
Assuntos
Ferro/intoxicação , Tentativa de Suicídio , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Intoxicação/terapiaRESUMO
BACKGROUND: The acceptance of a colostomy by a patient requires a great amount of psychological resources and social support. AIM: To identify factors that influence the postoperative adaptation to a colostomy. PATIENTS AND METHODS: Sixty patients subjected to a colostomy in 5 hospitals in Santiago were interviewed. The adaptation to the procedure was assessed using the Olsbrisch Ostomy Adjustment Scale. Three regression equations were elaborated to determine the main predictors associated to adaptation. Control variables such as sex, age and the lapse between the interview and the surgical procedure, were included in the model. RESULTS: The model used identified the simultaneous and combined effects of socioeconomic variables such as education and income on the adaptation to colostomy. A generalized detriment of the self image was detected. Patients valued the social support given by the family and friends. Multiple regression analysis determined that the main predictor of the patient's adaptation to colostomy was the level of self care developed. CONCLUSIONS: The better adaptation to a colostomy could be achieved by training the patient for an adequate self care and providing him with psychological and social support.
Assuntos
Adaptação Psicológica , Colostomia/psicologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Chile , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Ajustamento SocialRESUMO
PURPOSE: Factors contributing to the postoperative adjustment of patients who had undergone permanent colostomy surgery were studied. DESIGN: Cross-sectional study. SETTING AND SUBJECTS: Sixty patients who underwent colostomy surgery at 5 hospitals in Santiago, Chile, between 1987 and 1995 were evaluated. Subjects were between the ages of 27 and 89 and underwent colostomy surgery from 4 months to 19 years before data collection. METHODS AND INSTRUMENTS: A demographic questionnaire and a semistructured interview were used to elicit information concerning psychosocial reactions to a colostomy and perceptions about the changes in lifestyle created by the ostomy. Medical records were reviewed for information about disease-related characteristics and an ostomy self-care scale measured coping skills. Adjustment to the colostomy procedure was measured by means of the Olbrisch ostomy adjustment scale. RESULTS: Patient adaptation to a colostomy is primarily affected by the following factors: (1) the level of ostomy self-care, (2) psychological support, and (3) social support from family and significant others. CONCLUSION: Successful adjustment to a permanent colostomy is most likely to occur if the patient receives adequate instruction in self-care and has the appropriate psychological support to integrate the new physical changes into a healthy body image. This includes continued acceptance from family and the patient's social network.
Assuntos
Adaptação Psicológica , Colostomia/psicologia , Modelos Psicológicos , Adulto , Idoso , Idoso de 80 Anos ou mais , Imagem Corporal , Colostomia/enfermagem , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Testes Neuropsicológicos , Valor Preditivo dos Testes , Qualidade de Vida , Análise de Regressão , Autocuidado , Apoio Social , Inquéritos e QuestionáriosRESUMO
Apoptosis is a mechanism of cell death that occurs in normal development and on the regulation of vertebrate tissues and organ cellularity. Neurons undergo p53-dependent and p53-independent apoptosis, depending upon the stimulus that triggers DNA fragmentation. Many neurons in the developing nervous system suffer apoptosis, with the cyclin D1 being an essential mediator of neuronal cell death. Other characteristics of apoptosis are: condensation of the nucleus, fragmentation of chromatin at nucleosome linkage sites, membrane blebbing, and the formation of apoptotic bodies. Among the possible molecular mechanisms are: (a) activation of proteases, as ICE (Il-1 beta converting enzyme); (b) calpain is activated in several cells, with PARP (Poly-ADP-ribose polymerase) and a small U1 Ribonucleoprotein, being substrates for ICE and its homologs such as ICH and others proteins. The p53 gene encodes a transcription factor that contributes to several different cellular activities, including apoptosis, the cellular response to radiation, and the activation of proteins such as GADD, Bcl-2 (represses to apoptosis) and Bax. P53 exerts a role as inductor of apoptosis by transactivating expression of the Bax gene. The p53 gene tumor suppressor limits cellular proliferation by including either the arrest of cell cycle in G1, or apoptosis, depending on the cellular context. The p21 is an inhibitor of cyclin-dependent kinase, which is transactivated by p53. During apoptosis, there is an activation of both, c-myc, and the transcription factor NF-kB, which is a important regulator of apoptosis. As an example of signalization of apoptosis we have selected to illustrate the problem related to the system Fas/APO in thymocytes.
Assuntos
Morte Celular/genética , Genes p53/genética , Animais , Apoptose/genética , Ciclo Celular , Neoplasias Colorretais/genética , Cisteína Endopeptidases/genética , Citoesqueleto/genética , Genes myc/genética , Humanos , Camundongos , Mutação , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteína do Retinoblastoma/genética , Transcrição Gênica , Ativação TranscricionalRESUMO
Aspiration of gastric contents to the lung is a cause of Adult Respiration Distress syndrome. We studied the effects of endotracheal instillation of hydrochloric acid (2 ml/kg, 0.1 N solution) in anesthetized rats who were sacrificed 4 or 48 hr later. A control group received 0.9% saline solution instead. Total protein, phospholipids, disaturated phosphatidylcholine (DSPC) and number of cells were determined in the bronchoalveolar lavage fluid. Histologic studies were performed in one rat in each group. HCl caused edema and inflammatory reaction characterized by a rise in protein and cells in the bronchoalveolar fluid 48 hr after endotracheal instillation. This was associated with an increase in total phospholipid and in DSPC. Therefore, the inflammatory response to HCl is associated to an increase in alveolar surfactant 48 hr after instillation.
Assuntos
Ácido Clorídrico/efeitos adversos , Pulmão/efeitos dos fármacos , Surfactantes Pulmonares/efeitos dos fármacos , Animais , Peso Corporal , Pulmão/patologia , Tamanho do Órgão , Ratos , Ratos Endogâmicos , Cloreto de Sódio/efeitos adversosRESUMO
A case of liver actinomycosis is presented. It was diagnosed 15 days after the patient had undergone appendicectomy. Emphasis is made concerning the diagnostic difficulties in the clinical, radiological and bacteriological areas. In the present paper note is made of the low frequency of this disease in Mexico. In this case it was possible arrive at a definitive diagnosis only after the histopathological study of material obtained in a needle liver biopsy which demonstrated the sulfur granules that are characteristic of the lesion. Information is given concerning the clinical and surgical evolution as well as therapeutic management. The case is illustrated with representative roentgenograms and a microphotograph of the lesion.