RESUMO
The authors report the enzyme histochemistry of the liver obtained from autopsy material in 22 corpses (2 to 12 hours post-mortem) and performed to evaluate the sensitivity of enzyme activities to the autolysis process and the use of enzymes to estimate time in forensic pathology. The earliest sample was at 2 hours post-mortem; there was five cases up to 5 hours; eight case up to 8 hours and eight cases up to 12 hours since death. Active phosphorylase (PHYLA a) and total phosphorylase (PHYLA t) were negative two hours after death. PHYLA t reaction represents the activity of PHYLA a increased with the inactive phosphorylase b which can be activated by the addition of ATP and Mg2+ to the incubation medium for phosphorylase a; this activation proved to be ineffective in the post-mortem periods of this study. Glucose-6-phosphatase (G6P-A) also showed a tendency to be sensitive to the autolysis process, displaying a reaction progressively weaker or negative in the post-mortem periods of observation. The results indicate these enzymes as a possible tool to estimate time in forensic pathology deserving further investigation. Lactate dehydrogenase (L-D), alpha-glycerophosphate dehydrogenase (alpha-GP-D) and beta-hydroxybutyrate dehydrogenase (beta-HOB-D) instead showed stronger reactions as the autolysis process evolved.
Assuntos
Fígado/enzimologia , Mudanças Depois da Morte , Adulto , Idoso , Autopsia , Feminino , Medicina Legal , Histocitoquímica , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de TempoRESUMO
An LH-RH agonist, des-Gly10, [DTrp6]-LH-RH ethylamide (LH-RH A), was administered chronically to adult male cats in order to determine its effect on the steroidogenesis of the testis during the stimulatory action of human chorionic gonadotropin (hCG). Measurement of plasma testosterone levels were combined with the histochemical analysis of some steps of the testicular steroidogenic pathway. Chronic daily treatment with LH-RH A(20 *g/kg) for 67 days inhibited the early testicular response to hCG during the initial 0.5,1 and 24 h, whereas the inhibitory egffect was not observed 48 and 72 h after hCG administration.The maximal responses to hCG were obtained both in LH-RH A-treated animals and in their control group 48 and 72 h after hCG adnministration. Under these conditions, LH-RH A-treated cats showed no alteration in 3ß-hydroxysteroid dehydrogenase (3ß-Host-D) activity, whereas a marked inhibition was observed in the activity of alcohol dehydrogenase (ADII) which reflects the activity of 20,22-desmolase and/or 17,20-desmolase.
Assuntos
Gatos , Animais , Masculino , Gonadotropina Coriônica/farmacologia , Hormônio Liberador de Gonadotropina/análogos & derivados , Esteroides/metabolismo , Testículo/fisiologia , Testosterona/metabolismo , Testosterona/sangueRESUMO
An LH-RH agonist, des-Gly10,[DTrp6]-LH-RH ethylamide (LH-RH A), was administered chronically to adult male cats in order to determine its effect on the steroidogenesis of the testis during the stimulatory action of human chorionic gonadotropin (hCG). Measurement of plasma testosterone levels were combined with the histochemical analysis of some steps of the testicular steroidogenic pathway. Chronic daily treatment with LH-RH A (20 micrograms/kg) for 67 days inhibited the early testicular response to hCG during the initial 0.5, 1 and 24 h, whereas the inhibitory effect was not observed 48 and 72 h after hCG administration. The maximal responses to hCG were obtained both in LH-RH A-treated animals and in their control group 48 and 72 h after hCG administration. Under these conditions, LH-RH A-treated cats showed no alteration in 3 beta-hydroxysteroid dehydrogenase (3 beta-Host-D) activity, whereas a marked inhibition was observed in the activity of alcohol dehydrogenase (ADII) which reflects the activity of 20,22-desmolase and/or 17,20-desmolase.
Assuntos
Gonadotropina Coriônica/farmacologia , Hormônio Liberador de Gonadotropina/análogos & derivados , Testículo/metabolismo , Testosterona/metabolismo , Animais , Gatos , Hormônio Liberador de Gonadotropina/administração & dosagem , Masculino , Testículo/efeitos dos fármacos , Testosterona/sangueRESUMO
An LH-RH analog (des-Gly10,[D-Trp6]-LH-RH ethylamide, LH-RH A) was administered to adult male cats for 67 days (20 micrograms/kg, sc) in order to study its inhibitory effects on the structure of Leydig cells, as determined by histological and histochemical-morphometric techniques. Histological examination showed that LH-RH A promotes a decrease in the volume of the interstitial tissue. In addition, Leydig cell nuclei exhibited marked structural alterations. Morphometric analyses utilizing histochemistry of the enzyme 3-beta-hydroxysteroid dehydrogenase (3-beta-HOST-D) as a marker of Leydig cells also demonstrated a significant decrease of the relative volume occupied by the Leydig cells in the testis.
Assuntos
3-Hidroxiesteroide Desidrogenases/metabolismo , Hormônio Liberador de Gonadotropina/análogos & derivados , Células Intersticiais do Testículo/ultraestrutura , Testículo/efeitos dos fármacos , Animais , Gatos , Hormônio Liberador de Gonadotropina/farmacologia , Células Intersticiais do Testículo/efeitos dos fármacos , MasculinoRESUMO
The effects of administration of 1.25 mg testosterone propionate between the 2nd and 5th day of life on body weight, ovarian weight, food and water consumption, and the histochemical activity of 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) were studied in female Wistar rats during the evolution of experimental micropolycystic ovaries. Biometric studies showed a 9.9 day delay of vaginal opening for the treated animals and that the increased body weight of the animals during the disease was not related to food intake and thus presumably due to the induced metabolic disorder. Reduced histochemical activity of the 17 beta-HSD of testosterone-treated rats was detected at ages 30, 60 and 90 days before the onset of morphological alterations of the ovaries. The possible participation of 17 beta-HSD in the disequilibrium between androstenedione and testosterone and in the genesis of the disease is discussed.
Assuntos
17-Hidroxiesteroide Desidrogenases/metabolismo , Peso Corporal/efeitos dos fármacos , Ovário/efeitos dos fármacos , Síndrome do Ovário Policístico/fisiopatologia , Maturidade Sexual/efeitos dos fármacos , Testosterona/farmacologia , Animais , Feminino , Tamanho do Órgão/efeitos dos fármacos , Ovário/patologia , Ratos , Ratos EndogâmicosAssuntos
Ratos , Animais , Feminino , 17-Hidroxiesteroide Desidrogenases/metabolismo , Peso Corporal/efeitos dos fármacos , Tamanho do Órgão/efeitos dos fármacos , Ovário/efeitos dos fármacos , Síndrome do Ovário Policístico/fisiopatologia , Maturidade Sexual/efeitos dos fármacos , Testosterona/farmacologia , Ovário/patologia , Ratos EndogâmicosRESUMO
Após breve revisäo da legislaçäo brasileira referente à pós-graduaçäo na área médica, descreve-se a adaptaçäo dessa legislaçäo aos programas de Mestrado e Doutorado em Patologia Humana da Faculdade de Medicina de Ribeiräo Preto, bem como a experiência acumulada nos últimos 6 anos e as perspectivas desses programas