RESUMO
This study aimed to evaluate the chemical composition and cytotoxic activity of brown Brazilian propolis and its in vitro activity against itraconazole-resistant Sporothrix brasiliensis from animal sporotrichosis. Propolis was acquired commercially and prepared as a hydroalcoholic extract. Chemical analysis was evaluated by liquid chromatography coupled to mass spectrometry of ultra-efficiency. The cell viability was evaluated by MTT test in MDBK cells of 50 to 0.09 µg/mL. For antifungal tests, twenty isolates of Sporothrix brasiliensis from dogs (n = 11) and cats (n = 9) with sporotrichosis were tested to itraconazole (16-0.0313 µg/mL) and to propolis (3.125-0.09 mg/mL) by broth microdilution technique (CLSI M38-A2), adapted to natural products. The results were expressed in minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). Itraconazole showed activity between MIC values of 0.25 to greater than 16 µg/mL, and 88.9% (08/09) and 72.7% (08/11) of S. brasiliensis from cats and dogs, respectively, were considered itraconazole-resistant. All Sporothrix brasiliensis were sensitive to brown propolis between MIC values of 0.19-1.56 mg/mL, including the itraconazole-resistant isolates, whereas the MFC values of propolis were from 0.78 to greater than 3.125 mg/mL. Propolis maintained a medium to high cell viability between concentration of 0.78 to 0.09 µg/mL, and p-coumaric acid was the major compound. Brown Brazilian propolis is a promising antifungal candidate against sporotrichosis and more studies need to be undertaken to evaluate its safe use to understand its efficacy.
Assuntos
Antifúngicos/farmacologia , Antifúngicos/toxicidade , Produtos Biológicos/farmacologia , Produtos Biológicos/toxicidade , Própole/farmacologia , Própole/toxicidade , Sporothrix/efeitos dos fármacos , Animais , Antifúngicos/química , Antifúngicos/isolamento & purificação , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Brasil , Doenças do Gato/microbiologia , Gatos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida , Doenças do Cão/microbiologia , Cães , Farmacorresistência Fúngica , Itraconazol/farmacologia , Espectrometria de Massas , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Sporothrix/isolamento & purificação , Esporotricose/veterináriaRESUMO
Lantana camara L. (Verbenaceae) possesses several medicinal properties and it is used in folk medicine with antipyretic, antimicrobial and antimutagenic properties. This plant is one of the 10 most noxious weeds in the world. Lantana poisoning have caused severe economic losses and was the major cause of livestock mortality and morbidity. In this article we report the effects of hydroalcoholic extract from Lantana camara var. aculeata leaves on fertility, general reproductive performance and teratology in the rat. The data showed that the extract interfered in the frequency of fetal skeleton anomalies from dams treated with the extract and induced embryotoxicity as indicated by post-implantation loss, without any signs of maternal toxicity. The other parameters evaluated did not suggest modifications.