RESUMO
11 beta-hydroxysteroid dehydrogenase (HSDs) enzymes regulate the activity of glucocorticoids in target organs. HSD1, one of the two existing isoforms, locates mainly in CNS, liver and adipose tissue. HSD1 is involved in the pathogenesis of diseases such as obesity, insulin resistance, arterial hypertension and the Metabolic Syndrome. The stress produced by HCl overload triggers metabolic acidosis and increases liver HSD1 activity associated with increased phosphoenolpyruvate carboxykinase, a regulatory enzyme of gluconeogenesis that is activated by glucocorticoids, with increased glycaemia and glycogen breakdown. The aim of this study was to analyze whether the metabolic modifications triggered by HCl stress are due to increased liver HSD1 activity. Glycyrrhetinic acid, a potent HDS inhibitor, was administered subcutaneously (20 mg/ml) to stressed and unstressed four months old maleSprague Dawley rats to investigate changes in liver HSD1, phosphoenolpyruvate carboxykinase (PECPK) and glycogen phosphorylase activities and plasma glucose levels. It was observed that all these parameters increased in stressed animals, but that treatment with glycyrrhetinic acid significantly reduced their levels. In conclusion, our results demonstrate the involvement of HSD1 in stress induced carbohydrate disturbances and could contribute to the impact of HSD1 inhibitors on carbohydrate metabolism and its relevance in the study of Metabolic Syndrome Disorder and non insulin-dependent diabetes mellitus.
Assuntos
11-beta-Hidroxiesteroide Desidrogenases/biossíntese , 11-beta-Hidroxiesteroide Desidrogenases/fisiologia , Glucose/metabolismo , Fígado/enzimologia , Tecido Adiposo/metabolismo , Animais , Metabolismo dos Carboidratos , Carboidratos/química , Sistema Nervoso Central/embriologia , Ácido Glicirretínico/metabolismo , Fígado/metabolismo , Masculino , Modelos Biológicos , Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo , Isoformas de Proteínas , Ratos , Ratos Sprague-DawleyRESUMO
We evaluated the porphyrinogenic ability of ethanol (20% in drinking water) per se, its effect on the development of sporadic porphyria cutanea tarda induced by hexachlorobenzene in female Wistar rats (170-190 g, N = 8/group), and the relationship with hepatic damage. Twenty-five percent of the animals receiving ethanol increased up to 14-, 25-, and 4.5-fold the urinary excretion of delta-aminolevulinate, porphobilinogen, and porphyrins, respectively. Ethanol exacerbated the precursor excretions elicited by hexachlorobenzene. Hepatic porphyrin levels increased by hexachlorobenzene treatment, while this parameter only increased (up to 90-fold) in some of the animals that received ethanol alone. Ethanol reduced the activities of uroporphyrinogen decarboxylase, delta-aminolevulinate dehydrase and ferrochelatase. In the ethanol group, many of the animals showed a 30% decrease in uroporphyrinogen activity; in the ethanol + hexachlorobenzene group, this decrease occurred before the one caused by hexachlorobenzene alone. Ethanol exacerbated the effects of hexachlorobenzene, among others, on the rate-limiting enzyme delta-aminolevulinate synthetase. The plasma activities of enzymes that are markers of hepatic damage were similar in all drug-treated groups. These results indicate that 1) ethanol exacerbates the biochemical manifestation of sporadic hexachlorobenzene-induced porphyria cutanea tarda; 2) ethanol per se affects several enzymatic and excretion parameters of the heme metabolic pathway; 3) since not all the animals were affected to the same extent, ethanol seems to be a porphyrinogenic agent only when there is a predisposition, and 4) hepatic damage showed no correlation with the development of porphyria cutanea tarda.
Assuntos
Etanol/farmacologia , Ferroquelatase/efeitos dos fármacos , Fígado/efeitos dos fármacos , Porfiria Cutânea Tardia/induzido quimicamente , Solventes/farmacologia , Uroporfirinogênio Descarboxilase/efeitos dos fármacos , Animais , Sistema Enzimático do Citocromo P-450/análise , Modelos Animais de Doenças , Feminino , Ferroquelatase/metabolismo , Hexaclorobenzeno , Fígado/enzimologia , Fígado/patologia , Porfobilinogênio/urina , Sintase do Porfobilinogênio/urina , Porfiria Cutânea Tardia/enzimologia , Porfiria Cutânea Tardia/urina , Porfirinas/urina , Ratos , Ratos Wistar , Uroporfirinogênio Descarboxilase/metabolismoRESUMO
We evaluated the porphyrinogenic ability of ethanol (20 percent in drinking water) per se, its effect on the development of sporadic porphyria cutanea tarda induced by hexachlorobenzene in female Wistar rats (170-190 g, N = 8/group), and the relationship with hepatic damage. Twenty-five percent of the animals receiving ethanol increased up to 14-, 25-, and 4.5-fold the urinary excretion of delta-aminolevulinate, porphobilinogen, and porphyrins, respectively. Ethanol exacerbated the precursor excretions elicited by hexachlorobenzene. Hepatic porphyrin levels increased by hexachlorobenzene treatment, while this parameter only increased (up to 90-fold) in some of the animals that received ethanol alone. Ethanol reduced the activities of uroporphyrinogen decarboxylase, delta-aminolevulinate dehydrase and ferrochelatase. In the ethanol group, many of the animals showed a 30 percent decrease in uroporphyrinogen activity; in the ethanol + hexachlorobenzene group, this decrease occurred before the one caused by hexachlorobenzene alone. Ethanol exacerbated the effects of hexachlorobenzene, among others, on the rate-limiting enzyme delta-aminolevulinate synthetase. The plasma activities of enzymes that are markers of hepatic damage were similar in all drug-treated groups. These results indicate that 1) ethanol exacerbates the biochemical manifestation of sporadic hexachlorobenzene-induced porphyria cutanea tarda; 2) ethanol per se affects several enzymatic and excretion parameters of the heme metabolic pathway; 3) since not all the animals were affected to the same extent, ethanol seems to be a porphyrinogenic agent only when there is a predisposition, and 4) hepatic damage showed no correlation with the development of porphyria cutanea tarda
Assuntos
Animais , Feminino , Ratos , Etanol , Ferroquelatase , Fígado , Porfiria Cutânea Tardia , Uroporfirinogênio Descarboxilase , /análise , Modelos Animais de Doenças , Ferroquelatase , Hexaclorobenzeno , Fígado , Porfobilinogênio , Sintase do Porfobilinogênio , Porfiria Cutânea Tardia , Porfirinas , Ratos Wistar , Uroporfirinogênio DescarboxilaseRESUMO
Rat hepatic coproporphyrinogen oxidase, the sixth enzyme in the heme biosynthetic pathway, was purified 1340-fold with a yield of 39.7%. To obtain the soluble enzyme, different methods were applied to disrupt mitochondria, with sonication giving the highest yield (85%). The minimum catalytic form of enzyme was a dimer with a molecular mass of 77 +/- 4 kDa. The existence of aggregated forms was possible since in fractions of gel filtration elution activity was observed with higher molecular mass. We determined a Stokes radius of 36.3 A, a sedimentation coefficient (S20,w) of 5.06 S, and frictional ratio of 1.29, suggesting a nearly globular shape of the protein. Regardless of the type of salt, high ionic strength inhibits the enzyme, probably modifying its native structure. Experiments with amino acid modifiers showed that histidine, arginine, and tryptophan are involved in the catalytic process. Non-ionic detergents and phospholipids activated the enzyme, probably because they reproduce its natural hydrophobic environment. The present study describes a simple method for the purification of rat liver coproporphyrinogen oxidase, introducing for the first time data on the structure and function of the protein in a tissue often used as a laboratory model in biological studies, and contributing to the study of human hereditary coproporphyria.
Assuntos
Coproporfirinogênio Oxidase/química , Coproporfirinogênio Oxidase/fisiologia , Fígado/enzimologia , Animais , Cromatografia Líquida de Alta Pressão , Coproporfirinogênio Oxidase/metabolismo , Detergentes/farmacologia , Dietil Pirocarbonato/metabolismo , Humanos , Octoxinol/farmacologia , Fenilglioxal/farmacologia , Fosfolipídeos/metabolismo , Polissorbatos/farmacologia , Ratos , Ratos Sprague-Dawley , Água/metabolismoRESUMO
Coproporphyrinogen oxidase, the sixth enzyme in the biosynthetic heme pathway, catalyzes the oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX. A reversed-phase high pressure liquid chromatography method was developed to measure coproporphyrinogen oxidase enzymatic activity in rat liver. With this method, the separation, identification and quantification of coproporphyrin III (oxidized substrate) and protoporphyrin IX (oxidized product) present in the assays could be carried out with no need of derivatization and in less than 15 min. Rat and human liver coproporphyrinogen oxidase basal activities determined using this method were 0.41+/-0.05 nmol of protoporphyrin IX/h per mg of hepatic protein and 0.87+/-0.06 protoporphyrin IX/h per mg of hepatic protein, respectively. Kinetic studies showed that optimum pH for rat CPGox is 7.3, and that its activity is linear in the range of protein concentrations and incubation times assayed. The present paper describes a sensitive, specific and rapid fluorometric high performance liquid chromatography method to measure coproporphyrinogen oxidase, which could be applied to the diagnosis of human coproporphyria, and which is also suitable for the study of lead and other metal poisoning that produce alterations in this enzymatic activity.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Coproporfirinogênio Oxidase/análise , Coproporfirinogênio Oxidase/metabolismo , Adulto , Animais , Calibragem , Coproporfirinas/análise , Feminino , Humanos , Concentração de Íons de Hidrogênio , Cinética , Fígado/enzimologia , Masculino , Protoporfirinas/análise , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
Porphobilinogen deaminase was purified from human hepatocytes. A variety of group specific reagents have been used to achieve site-specific modifications to evaluate the potential role of such groups in the whole catalytic cycle. Treatment with dicarbonyl reagents caused a rapid loss in activity that was time and concentration, dependent. Protection experiments revealed that arginine residues are involved in the binding of the substrate. Treatment with Woodward's reagent K showed the fastest inactivation of deaminase (85% in 30 sec at 30mM) which was pH dependent and could be prevented by the presence of substrate, suggesting that deprotonated carboxylated groups from Asp/Glu are essential for catalytic activity. Kinetic analysis gave values of 0.3 sec-1 for the k3 rate constant and 8x10-2 M for the KI of the non covalent complex between deaminase-Woodward's Reagent K.
RESUMO
A basal level of human liver porphobilinogen deaminase of 3.66 units/g wet weight was found in adult tissue. Activity in neonatal liver was at least three fold higher. Physico-chemical studies revealed that the enzyme has the approximate form of a symmetrical molecule and exhibits hyperbolic kinetics with a Km value of 3.6 microM at pH 7.6. Two ionizable groups with pK values of 7.35 and 8.90 are prominent for catalysis. A set of pI (5.8-4.9) were observed under different conditions. Results demonstrate the existence of a single protein differentially charged with multiple molecular forms in adult liver.
Assuntos
Hidroximetilbilano Sintase/metabolismo , Fígado/enzimologia , Adulto , Humanos , Concentração de Íons de Hidrogênio , Hidroximetilbilano Sintase/química , Recém-Nascido , Focalização Isoelétrica , CinéticaRESUMO
Porphobilinogen deaminase (porphobilinogen ammonia-lyase, EC 4.3.1.8) was isolated from rat liver. The final preparation was homogeneous according to polyacrylamide gel electrophoresis and immunodiffusion criteria. Electrophoresis of the native enzyme revealed a single band of activity which was distributed into three bands after incubation with porphobilinogen. When electrophoresed under denaturing condition it displayed a single polypeptide band with a molecular weight of 42,000 confirmed by exclusion chromatography and by sucrose density gradient centrifugation. The enzyme showed a pH optimum of 7.5 both in 0.1 M sodium phosphate and 0.05 M Tris-HCl buffer, when assayed at 37 degrees C. An isoelectric point of 4.9 for the native purified protein was found. Hepatic porphobilinogen deaminase was remarkably heat-stable showing maximum activity at 55-60 degrees C with one break in the Arrhenius plot. The kinetic behaviour of the purified enzyme followed the typical Michaelis-Menten kinetics with values of Km = 17 microM and Vmax = 29.4 units power mg in 0.1 M phosphate buffer at 37 degrees C. The amino acid composition was determined, showing that the enzyme had a low content of sulphur-containing amino acids and a considerable number of acidic residues per mol of polypeptide chain. Reagents known to interact with sulphydryl groups have small effect on rat liver enzyme activity.