RESUMO
ABSTRACT Objective: To describe the first known case of human Bertiellosis in Paraná (Brazil). Case description: A 6-year-old male residing in the Brazilian state of Paraná was suffering from intermittent nonspecific abdominal pain and abdominal distension, associated with expulsion of live tapeworms in his feces for 7 months. He had a history of interaction with monkeys on an island. His first feces analysis was inconclusive, with an initial hypothesis of an atypical Taenia. However, after additional research, the parasitologist identified pregnant proglottids of Bertiella sp. The patient was initially treated with an unknown dosage of albendazole and nitazoxanide, as it was believed he had been infected with Taenia sp. Since the symptoms persisted, praziquantel 10 mg/kg was prescribed without further proglottids elimination. Comments: Human Bertiellosis is a rare zoonosis, with less than one hundred cases reported. However, it is a cause of chronic abdominal pain and should be kept as a differential diagnosis, especially in cases reporting recurrent tapeworm expulsion in feces and refractory treatment with albendazole.
RESUMO Objetivo: Descrever o primeiro caso conhecido de bertielose humana no Paraná, Brasil. Descrição do caso: Criança de seis anos do sexo masculino, residente no Paraná, Brasil, apresentava dor abdominal inespecífica intermitente e distensão abdominal, associadas à expulsão de helmintos vivos em suas fezes havia sete meses. Tinha um histórico de interação com macacos em uma ilha. Sua primeira análise de fezes foi inconclusiva, com hipótese inicial de uma Taenia atípica. No entanto, após pesquisas adicionais, o parasitologista identificou proglótides gravídicas de Bertiella sp. O paciente foi inicialmente tratado com uma dosagem desconhecida de albendazol e nitazoxanida, pois se acreditava que havia sido infectado por Taenia sp. Diante da persistência dos sintomas, foi prescrito praziquantel 10 mg/kg, sem mais eliminação de proglótides. Comentários: A bertielose humana é uma zoonose rara, com menos de cem casos relatados. No entanto, é uma causa de dor abdominal crônica e deve ser mantida como diagnóstico diferencial, principalmente nos casos que relatam expulsão recorrente de helmintos nas fezes e que são refratários ao tratamento com albendazol.
RESUMO
OBJECTIVE: To describe the first known case of human Bertiellosis in Paraná (Brazil). CASE DESCRIPTION: A 6-year-old male residing in the Brazilian state of Paraná was suffering from intermittent nonspecific abdominal pain and abdominal distension, associated with expulsion of live tapeworms in his feces for 7 months. He had a history of interaction with monkeys on an island. His first feces analysis was inconclusive, with an initial hypothesis of an atypical Taenia. However, after additional research, the parasitologist identified pregnant proglottids of Bertiella sp. The patient was initially treated with an unknown dosage of albendazole and nitazoxanide, as it was believed he had been infected with Taenia sp. Since the symptoms persisted, praziquantel 10 mg/kg was prescribed without further proglottids elimination. COMMENTS: Human Bertiellosis is a rare zoonosis, with less than one hundred cases reported. However, it is a cause of chronic abdominal pain and should be kept as a differential diagnosis, especially in cases reporting recurrent tapeworm expulsion in feces and refractory treatment with albendazole.
Assuntos
Cestoides , Infecções por Cestoides , Masculino , Criança , Animais , Humanos , Brasil , Albendazol/uso terapêutico , Infecções por Cestoides/diagnóstico , Dor Abdominal/etiologiaRESUMO
Introdução: A crescente incidência de câncer de pele leva a um alto número de procedimentos cirúrgicos em todo o mundo. O principal objetivo do tratamento cirúrgico do câncer de pele é sua excisão completa, preservando a função e o melhor resultado estético. A ressecção inicial incompleta pode resultar em recorrências e danos graves. O objetivo é analisar os fatores de risco para margens positivas no seguimento de lesões cutâneas removidas cirurgicamente, por um ano, no departamento de cirurgia plástica do Hospital Royal Perth. Métodos: Foi analisado um levantamento de amostras histopatológicas de 947 pacientes operados. Todos os pacientes com excisão incompleta confirmada (EI) foram submetidos a uma segunda cirurgia ou até mesmo a uma terceira vez. Resultados: No total, 947 lesões foram encontradas, 6,6% das cirurgias tiveram margens comprometidas, com distribuição histopatológica de 75% de carcinoma basocelular (CBC), 21,4% de carcinoma de células escamosas (CCE) e 3,6% de outras lesões. A relação da presença de margens cirúrgicas comprometidas entre o CCE, quando comparada ao CBC, leva a um risco relativo de 2,8 e um valor p de 0,041, sugerindo que o primeiro é um fator de risco para a presença de margens cirúrgicas comprometidas. Para o estadiamento, a necessidade de uma segunda abordagem cirúrgica esteve presente em 61,29% dos pacientes, 20,9% estavam em observação, 3,2% estavam ausentes do serviço, 8% foram diretamente à quimioterapia ou radioterapia e 6,4% remarcaram a cirurgia. Conclusão: O conhecimento dos fatores de risco para margens positivas é necessário para que o cirurgião entenda o prognóstico e o acompanhamento de cada paciente.
Introduction: The increasing incidence of skin cancer leads to a high number of surgical procedures worldwide. The surgical treatment of skin cancer's main objective is its complete excision, preserving the function and the best aesthetic result. Incomplete initial resection can result in recurrences and major damage. The objective is to analyze the risk factors for positive margins in the follow-up of cutaneous lesions surgically removed, for one year, in the plastic surgery department of the Royal Perth Hospital. Methods: A survey of histopathological samples from 947 operated patients was analyzed. All patients with confirmed incomplete excision (IE) underwent a second surgery or even a third time. Results: In total, 947 lesions were found, 6.6% of surgeries had compromised margins, with a histopathological distribution of 75% of basal cell carcinoma, 21.4% of squamous cell carcinoma, and 3.6% of other lesions. The relation of the presence of compromised surgical margins between the SCC, compared to BCC, leads to a relative risk of 2.8 and a p-value of 0.041, which suggests that the SCC is a risk factor for the presence of compromised surgical margins. For staging, the need for a second surgical approach was present in 61.29% of the patients, 20.9% were under observation, 3.2% were absent from the service, 8% went directly to chemotherapy or radiotherapy, and 6.4% rescheduled the surgery. Conclusion: Knowledge of risk factors for positive margins is necessary for the surgeon to understand the prognosis and monitoring of each patient.
RESUMO
Introdução: A maior exposição nas mídias sociais gerou uma maior autoavaliação e a consequente busca pela aparência ideal. Dessa forma, a motivação de utilizar métodos cirúrgicos para melhora da aparência física cresce exponencialmente. Métodos: Foram distribuídos 3 questionários para 64 alunos: "Body Shape Questionnaire" (BSQ), "Escala de Investimento Corporal" (EIC) e "Questionário de interesse em cirurgias plásticas futuras" (QICPF). A partir da tabulação dos dados, foi realizada a análise estatística quantitativa por meio do teste qui-quadrado e risco relativo, para estudo objetivo e cruzado dos questionários. Resultados: O BSQ médio foi de 98,04, a relação de maior pontuação nesse questionário com QIRCP apresentou Risco Relativo (RR=2,41 e p=0,002). A média do EIC foi de 91 e, quando relacionado com QIRCP, não se apresentou estatisticamente relevante (RR=0,94 e p=0,83). Quanto à pergunta objetiva de satisfação corporal e o QIRCP, nota-se que a maior insatisfação corporal é fator de risco para querer realizar mais cirurgias plásticas (RR=1,94 e p=0,003) e as mulheres apresentam-se como o gênero de maior interesse (RR=3,01 e p=0,01). Conclusões: A análise estatística para a população estudada sugere que BSQ elevado, insatisfação corporal e mulheres apresentam maior interesse em realizar cirurgia plástica.
Introduction: Social media has generated an increase in selfevaluation and the search for the ideal appearance. Thus, the motivation to use surgical methods to improve physical appearance has grown exponentially. Methods: The Body Shape Questionnaire (BSQ), Body Investment Scale (BIS), and Questionnaire of Interest in Future Plastic Surgeries (QIFPS) were administered to 64 students. Once the data were collected, a quantitative statistical analysis using the chi-square test and relative risk (RR) was conducted. Results: The BSQ mean was 98.04, the relation of greater punctuation in this questionnaire with QIFPS presented an RR of 2.41; (p = 0.002). The mean BIS score was 91; compared to the QIFPS, it was not statistically relevant (RR = 0.94; p = 0.83). Regarding the objective question of body satisfaction and QIFPS, highest body dissatisfaction was a risk factor for desiring more plastic surgeries (RR = 1.94; p = 0.003), as was female sex (RR = 3.01; p = 0.01). Conclusions: Our findings suggest that a high BSQ score, high body dissatisfaction, and female sex are risk factors for a desire to undergo plastic surgery.
Assuntos
Humanos , História do Século XXI , Satisfação Pessoal , Cirurgia Plástica , Imagem Corporal , Interpretação Estatística de Dados , Inquéritos e Questionários , Estética , Aparência Física , Cirurgia Plástica/psicologia , Cirurgia Plástica/estatística & dados numéricos , Cirurgia Plástica/ética , Imagem Corporal/psicologia , Estética/psicologiaRESUMO
ABSTRACT Purpose: to analyze the hearing, language, motor and social skills of children and propose a screening of child development. Methods: 129 preschool children of both sexes, aged between three and six years old, enrolled in educational institutions and 25 teachers of kindergarten from public and private institutions, with no history of hearing disorders, with type A tympanometric curves and the presence of acoustic reflexes participated. For the children, the neuropsychomotor test, Denver II, and the evaluation of sound localization and temporal ordination of three verbal and non-verbal sounds were applied. For the educators responsible for the children, the Scale of Auditory Behaviors (SAB), was used. Results: most participants with normal SAB presented hearing abilities or standard Denver II; while in the amended SAB group, most participants presented alterations in Denver II or in the auditory abilities tests. It was found, also, that part of the children with standard Denver II were pointed, by the educators, as misbehaving in SAB. Conclusion: the combination of the findings of the Denver II, hearing tests of sound localization and temporal ordination and the SAB Scale is useful in the characterization of child development and, thus, the use of these three instruments for screening in this age group is recommended.
RESUMO Objetivo: analisar as habilidades auditivas, de linguagem, motoras e sociais de crianças e propor uma triagem do desenvolvimento infantil. Métodos: participaram 129 pré-escolares, de ambos os sexos, com idades entre três e seis anos, matriculadas em instituições de educação infantil e 25 educadores do ensino infantil oriundos de instituições públicas e particulares. Todas as crianças com curvas timpanométricas tipo A e presença de reflexos acústicos. Nas crianças, aplicou-se o teste de desenvolvimento neuropsicomotor, Denver II, e a avaliação da localização sonora e da ordenação temporal de três sons verbais e não verbais. Com as educadoras, foi aplicado a Escala SAB. Resultados: verificou-se que a grande maioria dos participantes com SAB normal apresentaram habilidades auditivas ou Denver II normal; enquanto no grupo de SAB alterado, grande parte dos participantes apresentou alteração no Denver II ou nos testes de habilidades auditivas. Foi constatado, ainda, que parte das crianças com Denver II normal foram apontados, pelos educadores, como com comportamento inadequado no SAB. Conclusão: a combinação entre os achados do Denver II, de testes auditivos de localização sonora e ordenação temporal e a Escala SAB são úteis na caracterização do desenvolvimento infantil, assim, recomenda-se o uso dos três instrumentos como triagem.
RESUMO
According to the experience of people with schizophrenia, their psychiatrists' attitude towards the outcome of their illness is lacking in hope, which directly affects mutual faith in treatment. Here we discuss the scientific basis of hope and show its instrumental role in optimising the best treatment strategies for schizophrenia. Declaration of interest R.A.B has received honoraria for educational input and non-financial support from Ache; honoraria for educational input from Lundbeck; grants, honoraria for educational input and non-financial support from Janssen; all outside the submitted work. G.E.M.G. has received honoraria for educational input and non-financial support from Janssen outside the submitted work. G.M. reports support from Janssen-Cilag, outside the submitted work, and is an employee at Janssen-Cilag. S.S. has received grants and honoraria for educational input from EnVivo Pharmaceuticals, Takeda, AbbVie and Janssen Pharmaceuticals, outside the submitted work.
Assuntos
Atitude do Pessoal de Saúde , Médicos , Psiquiatria , Esquizofrenia/terapia , Esperança , HumanosRESUMO
Epilepsy is a neurological disorder with significant prevalence and the individuals affected by this disease have a great probability of occurrence of a lethal phenomenon known as Sudden Unexpected Death in Epilepsy (SUDEP). SUDEP occurs mainly during the night and probably during sleep. The pathophysiological mechanisms involved in this lethal phenomenon are still obscure and new evidences that could corroborate in this area are warranted. Thus, the aim of the present study was to evaluate the effect of sleep deprivation in the expression of microRNA (miRNA) in the frontal cortex and heart tissues of adult male rats after 50days of saline (SAL) or pilocarpine-induced status epilepticus (PILO). Initially 389 miRNA expressions were evaluated between SAL and PILO groups by microarray. Subsequently, 3 differentially expressed miRNAs of each tissue were investigated after total sleep deprivation (TSD 6h) and paradoxical sleep deprivation (PSD 24h). Still, it was analyzed that the effects of sleep rebound with equivalent duration of PSD and TSD. There was a significant increase of miR-146a expression, an important inflammatory modulator in the frontal cortex of PILO rats when compared to SAL animals. Animals treated with pilocarpine were affected by TSD (through overexpression of miRNAs related to inflammatory process) and these changes were maintained even after a sleep window of 6h. In contrast, miRNAs associated with heart diseases were down-regulated in PSD rebound, suggesting a possible restoration of homeostasis in cardiovascular system of SAL and PILO groups.
Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , MicroRNAs/metabolismo , Agonistas Muscarínicos/toxicidade , Pilocarpina/toxicidade , Privação do Sono/metabolismo , Estado Epiléptico/induzido quimicamente , Análise de Variância , Animais , Modelos Animais de Doenças , Lobo Frontal/efeitos dos fármacos , Lobo Frontal/metabolismo , Masculino , MicroRNAs/genética , Análise em Microsséries , Ratos , Ratos Wistar , Estado Epiléptico/fisiopatologia , Fatores de TempoRESUMO
Animals exposed to an early adverse event may be more susceptible to a second source of stress later in life, and these stressors may have additive deleterious effects. Sleep deprivation is known to be a stressor, affecting multiple body functions such as the cognition. Modafinil enhances working memory and attention in healthy non-sleep deprived subjects and in animal models of sleep deprivation. The first aim of the present study was to investigate the effects of maternal separation (MS) combined with paradoxical sleep deprivation (PSD) in adulthood on recognition memory in rats. Second, we aimed to evaluate whether the administration of modafinil would be able to ameliorate memory deficits induced by MS and PSD. Wistar rat pups were initially distributed into MS and handling (H) groups, with their litters standardized in 4 females and 4 males. In adulthood, the male rats were submitted to PSD or control condition, being redistributed afterwards in modafinil- or vehicle-treatment immediately after the training session of object recognition task. PSD did not potentiate the cognitive deficit due to MS. However, modafinil was able to recover memory impairments associated to PSD and also to MS in the neonatal period. This study demonstrates for the first time that modafinil ameliorates cognitive deficits associated to MS and to PSD in adulthood, independent from MS in the neonatal period.
Assuntos
Ansiedade de Separação/tratamento farmacológico , Ansiedade de Separação/psicologia , Compostos Benzidrílicos/farmacologia , Estimulantes do Sistema Nervoso Central/farmacologia , Transtornos Cognitivos/tratamento farmacológico , Privação do Sono/tratamento farmacológico , Privação do Sono/psicologia , Análise de Variância , Animais , Transtornos Cognitivos/psicologia , Feminino , Manobra Psicológica , Modafinila , Gravidez , Ratos , Ratos Wistar , Reconhecimento Psicológico/efeitos dos fármacos , Estresse Psicológico/tratamento farmacológico , Estresse Psicológico/psicologiaRESUMO
The aim of this study was to investigate the association between physical activity levels (occupational, sports, and leisure time activities), depression, anxiety, and epilepsy. The behavioral outcomes of individuals with epilepsy (E) were also compared with healthy control subjects (C). The sample included 31 individuals with epilepsy (12 with idiopathic generalized epilepsy and 19 with partial epilepsy) and 31 control subjects. Self-rating questionnaires were used to assess mood (State-Trait Anxiety Inventory and Beck Depression Inventory), anxiety, and depression as well as habitual physical activity. Patients with epilepsy were more severely impaired compared to control subjects in both mood questionnaires and presented higher levels of depression (35%), state anxiety (18%), and trait anxiety (12.6%) when compared to the C group. Although physical activity level did not differ significantly between groups, linear regression analyses showed that the physical activity leisure level predicted 31% of depression levels and 26% of anxiety levels in the E group. These data suggest that low levels of physical activity may be considered a risk factor for the development of depression and anxiety and can play an important role in the quality of life of individuals with epilepsy.
Assuntos
Epilepsia/complicações , Atividades de Lazer/psicologia , Transtornos do Humor/etiologia , Transtornos do Humor/psicologia , Atividade Motora/fisiologia , Adolescente , Adulto , Feminino , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Escalas de Graduação Psiquiátrica , Inquéritos e Questionários , Adulto JovemRESUMO
Sleep and epilepsy present a bidirectional interaction. Sleep complaints are common in epilepsy, and sleep deprivation may provoke seizures. However, the mechanisms underlying this relationship are unknown. Thus, this study investigated the effects of paradoxical sleep deprivation (PSD24h) and total sleep deprivation (TSD6h) in the expression of genes related to reactive oxygen species and nitric oxide production in the frontal cortex of a rodent model of temporal lobe epilepsy (PILO). The data show that PILO rats had increased NOX-2 expression and decreased SOD expression, independent of sleep. Higher NOX-2 expression was observed only in PILO rats subjected to the control condition and TSD6h. Also, eNOS and DDAH1 were increased in the PILO group submitted to TSD6h. Moreover, CAT expression in the frontal cortex of PILO rats submitted to PSD24h was reduced compared to that of PILO rats that were not sleep-deprived. The molecular changes found in the frontal cortex of PILO rats following sleep deprivation suggest a mechanism via oxidative stress.
Assuntos
Lobo Frontal/metabolismo , Regulação da Expressão Gênica/fisiologia , Privação do Sono , Estado Epiléptico/patologia , Amidoidrolases/genética , Amidoidrolases/metabolismo , Análise de Variância , Enzima de Conversão de Angiotensina 2 , Animais , Catalase/genética , Catalase/metabolismo , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Agonistas Muscarínicos/toxicidade , NADPH Oxidase 2 , NADPH Oxidases/genética , NADPH Oxidases/metabolismo , Óxido Nítrico Sintase Tipo III/genética , Óxido Nítrico Sintase Tipo III/metabolismo , Peptidil Dipeptidase A/genética , Peptidil Dipeptidase A/metabolismo , Pilocarpina/toxicidade , RNA Mensageiro/metabolismo , Ratos , Sono REM , Estado Epiléptico/induzido quimicamente , Superóxido Dismutase/metabolismo , Fatores de TempoRESUMO
The relationship between sleep and epilepsy has been well established. There is a high prevalence of sleep disturbances in epilepsy, which are associated with a decreased quality of life of individuals with epilepsy. In view of this fact, preclinical research is necessary to address many gaps in knowledge. For instance, it is well known that sleep deprivation can trigger seizures; however, this is a complex pathophysiological event. In this context, there are many valuable animal models of epilepsy that reproduce clinical symptoms and can be used. Investigations using animal models that simulate clinical epilepsy are imperative. Furthermore, preclinical studies that reveal mechanisms related to sleep-epilepsy interactions are very important. Results of such studies can, in turn, improve the understanding of epilepsy itself and can be useful in developing new antiepileptic drugs and preventive measures to control seizures. Preclinical research should be performed using a translational framework with experimental designs that can lead to advances in the quality of life of individuals with epilepsy. In view of the fact that more than 50 million of people are affected by epilepsy around the world, understanding the relationship between sleep and epilepsy is imperative.
Assuntos
Epilepsia/complicações , Transtornos do Sono-Vigília/complicações , Transtornos do Sono-Vigília/etiologia , Sono/fisiologia , Animais , Anticonvulsivantes/farmacologia , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Epilepsia/etiologia , Epilepsia/terapia , Humanos , Sono/efeitos dos fármacosRESUMO
Although the influence of sex on sleep pattern and cardiovascular parameters is well known, knowledge regarding the effects of sleep loss on heart responses in both sexes is scarce. The present study investigated the effects of paradoxical sleep deprivation (PSD) and chronic sleep restriction (SR) on cardiovascular parameters and adrenocorticotropic hormone (ACTH) levels in male and female rats. Both groups were randomly assigned to PSD for 96 h, SR for 21 days or home-cage control. Mean arterial pressure (MAP), heart rate (HR), baroreflex sensitivity (bradycardia and tachycardia responses) and ACTH levels were evaluated. The results showed that PSD induced a significant increase in HR and ACTH levels in both sexes, although male rats presented higher levels of ACTH hormone compared to females. In addition to sex-specific responses, PSD decreased the tachycardia only in male rats. SR, induced a significant increase in MAP and decrease in bradycardia in both sexes. Male rats were more affected by sleep deprivation protocols than females for MAP, bradycardia response, and ACTH levels. The results showed that the effects of sleep loss on cardiovascular parameters are associated with the protocol of sleep deprivation and that sex can modulate these effects. We suggested this experimental model as a suitable tool for further investigations of the relationship between cardiovascular parameters and sleep.
Assuntos
Pressão Sanguínea/fisiologia , Frequência Cardíaca/fisiologia , Hormônios/sangue , Caracteres Sexuais , Privação do Sono/sangue , Privação do Sono/fisiopatologia , Animais , Barorreflexo/efeitos dos fármacos , Barorreflexo/fisiologia , Pressão Sanguínea/efeitos dos fármacos , Cardiotônicos/farmacologia , Feminino , Frequência Cardíaca/efeitos dos fármacos , Masculino , Nitroprussiato/farmacologia , Fenilefrina/farmacologia , Ratos , Ratos Wistar , Estatísticas não Paramétricas , Vasodilatadores/farmacologiaRESUMO
INTRODUCTION: Epilepsy is a chronic disease that affects men and women of all ages, with different levels of severity. Many individuals with epilepsy also suffer from impairments in sexual function. However, it is difficult to differentiate between the impact of the disease and the impact of antiepileptic drugs on sexual function in human subjects. AIMS: To evaluate sexual behavior in adult male rats submitted to chronic pilocarpine-induced epilepsy. METHODS: First, non-epileptic rats were exposed to nine training sessions to acquire sexual experience, and their baseline sexual performance was evaluated. Then, the same rats were given pilocarpine to induce status epilepticus followed by chronic epilepsy. Once the animals had developed spontaneous recurrent seizures, their sexual behavior was evaluated during three sessions. MAIN OUTCOME MEASURES: Examine changes in latencies to first mount, intromission, and ejaculation, and the total number of mounts, intromissions, and ejaculations. RESULTS: All outcome measures related to sexual motivation and sexual performance were markedly impaired during chronic epilepsy compared with the baseline and the control group. CONCLUSION: These findings will aid in understanding the interaction between sexual behavior and epilepsy, as well as encouraging further experimental studies in human patients with epilepsy suffering from sexual dysfunction.
Assuntos
Epilepsia do Lobo Temporal/fisiopatologia , Comportamento Sexual Animal/fisiologia , Animais , Modelos Animais de Doenças , Epilepsia do Lobo Temporal/induzido quimicamente , Masculino , Agonistas Muscarínicos/farmacologia , Pilocarpina/farmacologia , Ratos , Ratos WistarRESUMO
INTRODUCTION: There is a high prevalence of erectile dysfunction (ED) in men with epilepsy; however, few studies have been conducted concerning the treatment of ED in this neurological group. AIM: The main purpose of this review is to highlight the influence of phosphodiesterase type 5 inhibitor (PDEI-5) for ED on seizure susceptibility. METHODS: All available online articles with information pertaining to PDEI-5 and seizure susceptibility were included in this review. MAIN OUTCOME MEASURES: The main outcome assessed demonstrated the intriguing role of PDEI-5 and its metabolites on seizure susceptibility. RESULTS: Case reports in men without epilepsy described seizure occurrence and electrophysiological changes following sildenafil, tadalafil, or vardenafil treatment. Consistent with these findings, preclinical studies suggested a proconvulsant effect of PDEI-5 on models of seizure induction. CONCLUSIONS: Evidence suggests an influence of PDEI-5 on seizure susceptibility in humans. In addition, preclinical studies have demonstrated the role of nitric oxide metabolites in the facilitation of paroxysmal phenomenon. Although there are many causes of seizures, medical professionals should be aware of the possible influence of PDEI-5 on seizure susceptibility. Further investigation by physicians and scientists is required to improve our understanding of this important topic.
Assuntos
Disfunção Erétil/tratamento farmacológico , Inibidores da Fosfodiesterase 5/efeitos adversos , Inibidores da Fosfodiesterase 5/uso terapêutico , Convulsões/induzido quimicamente , Idoso , Animais , Carbolinas/uso terapêutico , Suscetibilidade a Doenças , Disfunção Erétil/fisiopatologia , Humanos , Imidazóis/uso terapêutico , Masculino , Pessoa de Meia-Idade , Piperazinas/uso terapêutico , Prevalência , Purinas/uso terapêutico , Ratos , Citrato de Sildenafila , Sulfonas/uso terapêutico , Tadalafila , Triazinas/uso terapêutico , Dicloridrato de VardenafilaRESUMO
The interaction between sleep deprivation and epilepsy has been well described in electrophysiological studies, but the mechanisms underlying this association remain unclear. The present study evaluated the effects of sleep deprivation on locomotor activity and genetic damage in the brains of rats treated with saline or pilocarpine-induced status epilepticus (SE). After 50 days of pilocarpine or saline treatment, both groups were assigned randomly to total sleep deprivation (TSD) for 6 h, paradoxical sleep deprivation (PSD) for 24 h, or be kept in their home cages. Locomotor activity was assessed with the open field test followed by resection of brain for quantification of genetic damage by the single cell gel electrophoresis (comet) assay. Status epilepticus induced significant hyperactivity in the open field test and caused genetic damage in the brain. Sleep deprivation procedures (TSD and PSD) did not affect locomotor activity in epileptic or healthy rats, but resulted in significant DNA damage in brain cells. Although PSD had this effect in both vehicle and epileptic groups, TSD caused DNA damage only in epileptic rats. In conclusion, our results revealed that, despite a lack of behavioral effects of sleep deprivation, TSD and PSD induced genetic damage in rats submitted to pilocarpine-induced SE.
Assuntos
Hipercinese/fisiopatologia , Atividade Motora/fisiologia , Privação do Sono/fisiopatologia , Sono REM/fisiologia , Estado Epiléptico/fisiopatologia , Animais , Dano ao DNA , Hipercinese/genética , Masculino , Pilocarpina , Ratos , Ratos Wistar , Privação do Sono/genética , Estado Epiléptico/induzido quimicamente , Estado Epiléptico/genéticaRESUMO
The relationship between sleep and epilepsy has been known since ancient times, and the modulating effects of both on each other have been widely described in clinical studies. However, the mechanisms of this correlation remain unclear. Translational research is essential for filling the gaps in our knowledge, and for developing better therapeutic approaches to improve the quality of life of epileptic patients. Excellent animal models of epilepsy are available for the investigation of various aspects of epilepsy, such as epileptogenesis and hippocampal sclerosis. These models also show an association between sleep and epilepsy, suggesting that they are suitable for translational research on this relationship. While some knowledge has been obtained from preclinical studies, the topic remains relatively unexplored. In terms of the role of sleep in modulating seizure susceptibility in epilepsy, animal sleep research is a major tool. In this review, we focus on the intricate relationship between sleep and epilepsy in the preclinical setting, using a translational science approach.